FastQCFastQC Report
Fri 27 May 2016
SRR2139207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8908767
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145250.055.30751
GTATCAA268950.037.4669721
TCAACGC336200.029.646454
ATCAACG339750.029.3348663
TATCAAC345250.029.0026152
CAACGCA346800.028.7414285
AACGCAG357600.027.932686
ACGCAGA402050.024.8023077
CGCAGAG406550.024.5173478
GTGGTAT61000.024.0456871
TGGTATC59200.023.4162312
GTACATG359200.022.6007771
TACATGG362400.021.9216042
GCAGAGT465450.021.3083849
ACATGGG367550.021.0031533
GAGTACT267650.020.13389412-13
CAGAGTA456250.019.48836910-11
CATGGGG233950.019.2269384
AGAGTAC429850.018.64831710-11
GTACTTT289300.018.59083214-15
Produced by FastQC (version 0.11.5)