##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139207_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8908767
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62299844636188	33.0	33.0	33.0	33.0	33.0
2	32.62339681798839	33.0	33.0	33.0	33.0	33.0
3	32.62296028170902	33.0	33.0	33.0	33.0	33.0
4	36.54189182408744	37.0	37.0	37.0	37.0	37.0
5	36.570198210369625	37.0	37.0	37.0	37.0	37.0
6	36.58046427749205	37.0	37.0	37.0	37.0	37.0
7	36.55354506409248	37.0	37.0	37.0	37.0	37.0
8	36.547258111027034	37.0	37.0	37.0	37.0	37.0
9	36.57857288219571	37.0	37.0	37.0	37.0	37.0
10-11	36.52959438719185	37.0	37.0	37.0	37.0	37.0
12-13	36.51536278813892	37.0	37.0	37.0	37.0	37.0
14-15	38.588806228740744	40.0	37.0	40.0	37.0	40.0
16-17	38.528965063291025	40.0	37.0	40.0	37.0	40.0
18-19	38.56429324057976	40.0	37.0	40.0	37.0	40.0
20-21	38.56848447169461	40.0	37.0	40.0	37.0	40.0
22-23	38.56446453054756	40.0	37.0	40.0	37.0	40.0
24-25	38.560459847549154	40.0	37.0	40.0	37.0	40.0
26-27	38.43629365244688	40.0	37.0	40.0	37.0	40.0
28-29	38.38318350945161	40.0	37.0	40.0	37.0	40.0
30-31	38.22337608502339	40.0	37.0	40.0	37.0	40.0
32-33	38.23188574375038	40.0	37.0	40.0	37.0	40.0
34-35	38.20561991458993	40.0	37.0	40.0	37.0	40.0
36-37	38.1596794272472	40.0	37.0	40.0	37.0	40.0
38-39	38.10738919795516	40.0	37.0	40.0	35.0	40.0
40-41	38.07857166160728	40.0	37.0	40.0	33.0	40.0
42-43	38.031887954747674	40.0	37.0	40.0	33.0	40.0
44-45	37.955582888958006	40.0	37.0	40.0	33.0	40.0
46-47	37.806005421657844	40.0	37.0	40.0	33.0	40.0
48-49	37.82132760828168	40.0	37.0	40.0	33.0	40.0
50-51	37.78910351792967	40.0	37.0	40.0	33.0	40.0
52-53	37.731973601080234	40.0	37.0	40.0	33.0	40.0
54-55	37.5703247672635	40.0	37.0	40.0	33.0	40.0
56-57	37.45993444768407	40.0	37.0	40.0	33.0	40.0
58-59	37.336952686685905	38.5	37.0	40.0	33.0	40.0
60-61	37.17665607071342	37.0	37.0	40.0	33.0	40.0
62-63	37.00971092571912	37.0	37.0	40.0	33.0	40.0
64-65	36.8386814326899	37.0	37.0	40.0	33.0	40.0
66-67	36.63788475805328	37.0	37.0	40.0	33.0	40.0
68-69	36.429144512109424	37.0	37.0	40.0	33.0	40.0
70-71	36.198356056762165	37.0	37.0	38.5	33.0	40.0
72-73	35.97270290496826	37.0	37.0	37.0	33.0	40.0
74-75	35.776514227452125	37.0	37.0	37.0	33.0	40.0
76-77	34.093288167869446	35.0	33.0	37.0	30.0	37.0
78-79	35.27582973480064	37.0	33.0	37.0	33.0	37.0
80-81	35.32964663437379	37.0	33.0	37.0	33.0	37.0
82-83	35.18589922826905	37.0	33.0	37.0	33.0	37.0
84-85	35.058486726935655	37.0	33.0	37.0	33.0	37.0
86-87	34.97363773128285	37.0	33.0	37.0	33.0	37.0
88-89	34.842053942205155	37.0	33.0	37.0	33.0	37.0
90-91	34.698719700693545	37.0	33.0	37.0	33.0	37.0
92-93	34.70011113134386	37.0	33.0	37.0	33.0	37.0
94-95	34.7565321080598	37.0	33.0	37.0	33.0	37.0
96-97	34.76480638551696	37.0	33.0	37.0	33.0	37.0
98-99	34.770738867551415	37.0	33.0	37.0	33.0	37.0
100	34.76082695936854	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	4.0
19	14.0
20	79.0
21	335.0
22	1206.0
23	3453.0
24	7794.0
25	15329.0
26	26586.0
27	42011.0
28	61766.0
29	87614.0
30	117960.0
31	157628.0
32	209121.0
33	282025.0
34	389842.0
35	569695.0
36	1014563.0
37	2558592.0
38	3263068.0
39	100079.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00095254483589	20.14854580886446	14.389690514972497	23.46081113132715
2	14.672142620858756	21.120060722207686	41.906147057162904	22.301649599770652
3	18.111743185111926	26.77231316073257	30.557000761160324	24.55894289299518
4	12.390278026128644	18.29704379966386	38.84549904605205	30.467179128155447
5	13.184001781615795	38.00802063854628	35.30962253250085	13.49835504733708
6	29.384335625087694	37.94108039848464	18.976915953416583	13.697668023011083
7	26.182579474802743	33.058951929037995	23.18299490827406	17.575473687885204
8	24.458401482494715	36.53096999842963	21.174928023148436	17.835700495927213
9	26.039793394482853	16.598585774795946	21.100275964176856	36.261344866544356
10-11	24.322670207839206	26.78596215234335	28.657813248874447	20.233554390943002
12-13	25.34113875492282	24.58811069772297	28.752492629449762	21.318257917904447
14-15	22.420860235622065	25.685534078627203	26.765812798237658	25.127792887513074
16-17	21.57940038166898	28.81841561239619	27.393319412214957	22.20886459371987
18-19	21.56557691990373	27.686867329676485	29.505626311699473	21.24192943872031
20-21	22.650754031141883	26.775822869752297	29.346570327410237	21.226852771695583
22-23	22.342973137207597	26.821345686819324	29.198890682942398	21.636790493030677
24-25	21.98098392071339	27.067159390412414	29.31018132857431	21.64167536029989
26-27	21.873033019822273	27.19716776198758	29.263536783561435	21.666262434628713
28-29	21.713325816887995	27.215749311356223	29.308432893078233	21.76249197867754
30-31	22.146026231040974	27.110537787815307	29.12381954830538	21.619616432838335
32-33	21.8825766180145	27.25772962683129	29.094917928187748	21.76477582696646
34-35	22.011395509156	27.314677107546427	29.104836207860142	21.569091175437435
36-37	22.038419327778698	27.236220194430782	28.951030603928373	21.77432987386215
38-39	21.824078298382787	27.32659084828975	29.056850997191702	21.792479856135763
40-41	22.037840326000406	27.197960504097264	28.979066949811266	21.78513222009106
42-43	21.98162769902911	27.307429843960882	28.662821572259496	22.048120884750507
44-45	22.04400488011535	27.485808757210055	28.420290359390044	22.049896003284548
46-47	22.25087623824996	27.498553369116014	27.984549368579835	22.26602102405419
48-49	22.155499354707093	27.68626818719971	27.856455589363847	22.301776868729352
50-51	22.145977741703025	27.822636821452207	27.729983069173574	22.301402367671198
52-53	22.315458520698854	27.699669579524794	27.680863143945583	22.30400875583077
54-55	22.307961508867237	27.636842398015915	27.68737884305085	22.36781725006599
56-57	22.29427646123646	27.771810034633543	27.534517182458185	22.399396321671812
58-59	22.20738522127211	27.80064586019146	27.666567110197356	22.32540180833908
60-61	22.33375422968928	27.84424294560626	27.556842124823955	22.2651606998805
62-63	22.235567075836375	27.935751725464748	27.55092508491775	22.277756113781134
64-65	22.221651803471943	27.864160478808646	27.467274294270812	22.446913423448596
66-67	22.30655252359263	27.870101147477	27.334878602701878	22.48846772622849
68-69	22.29544631923582	27.809646320808678	27.435311270042558	22.459596089912946
70-71	22.31063849127109	27.623112795019246	27.473677186509672	22.5925715272
72-73	22.381835527500172	27.60759714328429	27.358619586300843	22.651947742914697
74-75	22.27684083782387	27.750209285709833	27.35049352147698	22.622456354989318
76-77	22.430653814658253	27.635324374135138	27.30797339739921	22.626048413807396
78-79	22.424949360813557	27.608018226615588	27.257790239882585	22.709242172688267
80-81	22.408502531234113	27.700078846145587	27.259541761658056	22.631876860962237
82-83	22.455470390267028	27.618096948861172	27.346626461064872	22.57980619980693
84-85	22.564824343134422	27.603405000760862	27.273133847137288	22.55863680896743
86-87	22.411403130127866	27.58917417782562	27.40386191051927	22.595560781527237
88-89	22.510313210314393	27.54893223475852	27.314826482201926	22.625928072725156
90-91	22.596134977063652	27.562096611067222	27.28357779451594	22.558190617353187
92-93	22.61892793554103	27.448420979041245	27.39654057971902	22.5361105056987
94-95	22.452636861996712	27.468030597451943	27.51455718945414	22.5647753510972
96-97	22.585886408289984	27.561580717694223	27.52049327202742	22.332039601988374
98-99	23.653301593737535	29.47197875620291	27.70653081422091	19.168188835838645
100	22.524356992605536	30.439997625678362	21.586464821228684	25.449180560487424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1928.0
1	1768.5
2	2674.0
3	3980.0
4	5458.0
5	9272.0
6	12551.0
7	14629.0
8	15742.0
9	15342.0
10	14234.5
11	13021.0
12	14172.0
13	18733.0
14	29726.0
15	45425.0
16	58626.5
17	67006.0
18	69238.0
19	64870.5
20	56423.0
21	47164.5
22	40470.5
23	38522.5
24	41804.0
25	50464.5
26	63441.5
27	78575.5
28	95261.5
29	114619.0
30	135463.5
31	157074.0
32	181573.5
33	205967.5
34	228828.5
35	251455.5
36	274493.5
37	294807.5
38	309526.0
39	321684.5
40	332349.0
41	340533.5
42	347318.5
43	354845.0
44	360880.0
45	366549.5
46	369644.0
47	369279.0
48	371313.5
49	367145.5
50	356279.5
51	345649.0
52	332016.0
53	315492.5
54	297617.0
55	275811.5
56	252240.0
57	228208.5
58	203247.5
59	176549.5
60	148342.0
61	121957.0
62	96895.5
63	74911.0
64	56920.5
65	43161.5
66	32793.5
67	24967.5
68	19192.0
69	13654.0
70	9359.0
71	6819.5
72	4819.5
73	3399.5
74	2376.5
75	1622.5
76	1087.0
77	688.5
78	434.0
79	298.0
80	212.5
81	152.5
82	121.0
83	85.5
84	53.5
85	35.5
86	25.0
87	18.5
88	13.0
89	8.0
90	7.0
91	7.0
92	5.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.9082326431929357E-4
7	0.0
8	0.0
9	2.2449795802269834E-5
10-11	0.0068079005770383255
12-13	2.2449795802269834E-5
14-15	0.006768613434384355
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	149.0
20-21	554.0
22-23	1212.0
24-25	4136.0
26-27	3846.0
28-29	7174.0
30-31	18163.0
32-33	22898.0
34-35	31774.0
36-37	45903.0
38-39	54486.0
40-41	59923.0
42-43	73410.0
44-45	88703.0
46-47	84950.0
48-49	75435.0
50-51	63817.0
52-53	58774.0
54-55	63638.0
56-57	70007.0
58-59	70173.0
60-61	70922.0
62-63	70698.0
64-65	74127.0
66-67	80874.0
68-69	80115.0
70-71	78828.0
72-73	75664.0
74-75	77480.0
76-77	77166.0
78-79	82260.0
80-81	84366.0
82-83	82288.0
84-85	81997.0
86-87	85714.0
88-89	92644.0
90-91	96074.0
92-93	99186.0
94-95	108418.0
96-97	289552.0
98-99	527011.0
100-101	5694258.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.79408927223665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.26087218627782	75.68794364680947
2	7.611111594976124	12.907545520907219
3	1.797164137124653	4.5716668884063
4	0.6321680996681377	2.1441647311328107
5	0.27582474422271985	1.1694153992556573
6	0.14660565691673638	0.7458775896247587
7	0.08194787783425235	0.4864086968123556
8	0.05005652009025559	0.3395597626552512
9	0.03244938927828157	0.24763647701629496
>10	0.10842521456679421	1.4208684603185737
>50	0.0027028326924563636	0.14815381448631743
>100	6.073330031751397E-4	0.09126993625809592
>500	6.441334854994675E-5	0.03948907631687993
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1224897901134915E-5	0.0
2	0.0	0.0	0.0	2.244979580226983E-5	0.0
3	0.0	0.0	0.0	2.244979580226983E-5	1.1224897901134915E-5
4	0.0	0.0	0.0	6.73493874068095E-5	1.1224897901134915E-5
5	0.0	0.0	0.0	6.73493874068095E-5	2.244979580226983E-5
6	0.0	0.0	0.0	7.857428530794441E-5	2.244979580226983E-5
7	0.0	1.1224897901134915E-5	0.0	1.0102408111021424E-4	4.489959160453966E-5
8	0.0	1.1224897901134915E-5	0.0	1.0102408111021424E-4	6.73493874068095E-5
9	0.0	4.489959160453966E-5	0.0	1.4592367271475392E-4	6.73493874068095E-5
10-11	0.0	4.489959160453966E-5	0.0	1.4592367271475392E-4	6.73493874068095E-5
12-13	0.0	4.489959160453966E-5	0.0	1.5714857061588882E-4	7.296183635737696E-5
14-15	0.0	1.1786142796191661E-4	0.0	1.9082326431929357E-4	1.2908632586305154E-4
16-17	0.0	1.5714857061588882E-4	0.0	2.469477538249681E-4	1.34698774813619E-4
18-19	0.0	1.6837346851702372E-4	0.0	2.806224475283729E-4	1.4592367271475392E-4
20-21	0.0	1.7959836641815864E-4	0.0	3.591967328363173E-4	1.6837346851702374E-4
22-23	0.0	2.0204816222042847E-4	0.0	4.6022081394653155E-4	1.6837346851702374E-4
24-25	0.0	2.2449795802269832E-4	0.0	0.0010046283621515748	1.6837346851702374E-4
26-27	0.0	2.3572285592383322E-4	0.0	0.003053172229108697	1.6837346851702374E-4
28-29	0.0	2.3572285592383322E-4	0.0	0.00832326179369154	1.852108153687261E-4
30-31	0.0	2.69397549627238E-4	0.0	0.010568241373918524	1.9082326431929357E-4
32-33	0.0	2.69397549627238E-4	0.0	0.015714857061588882	2.0766061117099595E-4
34-35	0.0	3.1429714123177763E-4	0.0	0.022371221516961887	2.2449795802269832E-4
36-37	0.0	3.591967328363173E-4	0.0	0.03302926207408949	2.2449795802269832E-4
38-39	0.0	3.591967328363173E-4	0.0	0.05977819377249399	2.4133530487440067E-4
40-41	0.0	3.9287142653972204E-4	0.0	0.08897415321334591	2.469477538249681E-4
42-43	0.0	4.097087733914244E-4	0.0	0.10740543556700943	2.525602027755356E-4
44-45	0.0	4.995079566005037E-4	0.0	0.12901897647564473	2.581726517261031E-4
46-47	0.0	5.612448950567458E-4	0.0	0.1509075273828578	2.581726517261031E-4
48-49	0.0	5.612448950567458E-4	0.0	0.17134245401187392	2.806224475283729E-4
50-51	0.0	6.398191803646902E-4	0.0	0.19090745105355209	2.8623489647894036E-4
52-53	0.0	6.398191803646902E-4	0.0	0.2401847528395344	3.0868469228121016E-4
54-55	0.0	6.566565272163925E-4	0.0	0.29666843907804524	3.1429714123177763E-4
56-57	0.0	6.959436698703648E-4	0.0	0.3526077177683511	3.255220391329126E-4
58-59	0.0	7.071685677714997E-4	0.0	0.3934495087816305	3.367469370340475E-4
60-61	0.0	7.183934656726346E-4	0.0	0.42339753638185845	3.479718349351824E-4
62-63	0.0	7.35230812524337E-4	0.0	0.4453253744317255	3.591967328363173E-4
64-65	0.0	7.857428530794441E-4	0.0	0.4517516284801253	3.591967328363173E-4
66-67	0.0	7.913553020300116E-4	0.0	0.4574931637565558	3.6480918178688476E-4
68-69	0.0	8.081926488817139E-4	0.0	0.463588283316872	3.928714265397221E-4
70-71	0.0	8.194175467828488E-4	0.0	0.46882469818775147	4.2093367129255937E-4
72-73	0.0	8.306424446839838E-4	0.0	0.4728768863300612	4.265461202431268E-4
74-75	0.0	8.474797915356861E-4	0.0	0.475980570599725	4.321585691936942E-4
76-77	0.0	8.643171383873884E-4	0.0	0.47901129303303136	4.377710181442617E-4
78-79	0.0	8.867669341896583E-4	0.0	0.48102054975733455	4.4338346709482917E-4
80-81	0.0	8.867669341896583E-4	0.0	0.48161546934609467	4.4899591604539665E-4
82-83	0.0	8.923793831402258E-4	0.0	0.4820644652621401	4.6022081394653155E-4
84-85	0.0	9.036042810413607E-4	0.0	0.4822721258733111	4.6583326289709897E-4
86-87	0.0	9.092167299919281E-4	0.0	0.48239559975022356	4.8267060974880135E-4
88	0.0	9.877910152998725E-4	0.0	0.48241243709707526	4.938955076499362E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14525	0.0	55.3075	1
GTATCAA	26895	0.0	37.466972	1
TCAACGC	33620	0.0	29.64645	4
ATCAACG	33975	0.0	29.334866	3
TATCAAC	34525	0.0	29.002615	2
CAACGCA	34680	0.0	28.741428	5
AACGCAG	35760	0.0	27.93268	6
ACGCAGA	40205	0.0	24.802307	7
CGCAGAG	40655	0.0	24.517347	8
GTGGTAT	6100	0.0	24.045687	1
TGGTATC	5920	0.0	23.416231	2
GTACATG	35920	0.0	22.600777	1
TACATGG	36240	0.0	21.921604	2
GCAGAGT	46545	0.0	21.308384	9
ACATGGG	36755	0.0	21.003153	3
GAGTACT	26765	0.0	20.133894	12-13
CAGAGTA	45625	0.0	19.488369	10-11
CATGGGG	23395	0.0	19.226938	4
AGAGTAC	42985	0.0	18.648317	10-11
GTACTTT	28930	0.0	18.590832	14-15
>>END_MODULE