FastQCFastQC Report
Fri 27 May 2016
SRR2139206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8449233
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173400.050.311481
GTATCAA312950.036.423221
ATCAACG389300.028.8706473
TCAACGC389500.028.8366874
TATCAAC395400.028.467662
CAACGCA395250.028.4508515
AACGCAG402900.028.0355246
ACGCAGA455450.024.7547457
CGCAGAG461250.024.425438
GTACATG415250.023.0988581
TACATGG419850.022.4064372
GTGGTAT78250.022.0192031
ACCGAAC16150.021.8080948
GCAGAGT518350.021.6072889
ACATGGG427000.021.4912663
CCGAACC17200.020.7223979
GAGTACT274500.020.29104812-13
AGGACCG24450.020.0639025
GGACCGA19800.019.4818866
CAGAGTA507300.019.45274410-11
Produced by FastQC (version 0.11.5)