FastQCFastQC Report
Fri 27 May 2016
SRR2139205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8313959
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105900.051.2686231
GTATCAA196850.039.3732341
TCAACGC239250.031.6323914
ATCAACG243250.031.1122283
TATCAAC245400.031.0699232
CAACGCA247100.030.5932125
AACGCAG252950.029.9028666
ACGCAGA289350.026.1262447
CGCAGAG312500.024.2048688
GTGGTAT54300.023.295841
GTACATG289350.022.805181
TACATGG290850.022.3737072
ACATGGG294550.021.6204033
GCAGAGT350550.021.5552439
TGGTATC54500.021.1365742
GAGTACT187450.019.7951212-13
AGAGTAC309550.019.09420210-11
CATGGGG227550.019.0650924
CAGAGTA335950.019.0560910-11
TATATAC83000.018.068673
Produced by FastQC (version 0.11.5)