FastQCFastQC Report
Fri 27 May 2016
SRR2139204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7854729
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106300.053.9846231
GTATCAA193950.038.9242741
TCAACGC238200.031.1608724
ATCAACG242950.030.5336323
CAACGCA246000.030.1921625
TATCAAC247250.030.1087532
AACGCAG251550.029.5434176
ACGCAGA289650.025.5819877
GTGGTAT50850.024.5145231
TGGTATC48750.024.0452732
CGCAGAG316250.023.4717648
GTACATG289400.022.8065831
TACATGG292600.022.3179872
GCAGAGT349200.021.159629
ACATGGG299950.021.0419983
GAGTACT178650.020.4952312-13
TATATAC78250.019.396113
CAGAGTA336950.019.38415110-11
CATGGGG228950.018.4928674
GTACTTT197550.018.45743414-15
Produced by FastQC (version 0.11.5)