##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139204_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7854729
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58981130984914	33.0	33.0	33.0	33.0	33.0
2	32.59222692469721	33.0	33.0	33.0	33.0	33.0
3	32.58695315904597	33.0	33.0	33.0	33.0	33.0
4	36.48855893054999	37.0	37.0	37.0	37.0	37.0
5	36.516290504739246	37.0	37.0	37.0	37.0	37.0
6	36.516065290094666	37.0	37.0	37.0	37.0	37.0
7	36.49140256780342	37.0	37.0	37.0	37.0	37.0
8	36.4734987037745	37.0	37.0	37.0	37.0	37.0
9	36.50938090925862	37.0	37.0	37.0	37.0	37.0
10-11	36.46497797696139	37.0	37.0	37.0	37.0	37.0
12-13	36.395603591161446	37.0	37.0	37.0	37.0	37.0
14-15	38.44348589238406	40.0	37.0	40.0	37.0	40.0
16-17	38.40315070067981	40.0	37.0	40.0	37.0	40.0
18-19	38.409806436351914	40.0	37.0	40.0	37.0	40.0
20-21	38.401465581738485	40.0	37.0	40.0	37.0	40.0
22-23	38.39385318861139	40.0	37.0	40.0	37.0	40.0
24-25	38.39378244741275	40.0	37.0	40.0	37.0	40.0
26-27	38.24782153663243	40.0	37.0	40.0	37.0	40.0
28-29	38.16834772044467	40.0	37.0	40.0	37.0	40.0
30-31	38.13113723941045	40.0	37.0	40.0	37.0	40.0
32-33	38.04832798186439	40.0	37.0	40.0	33.0	40.0
34-35	38.00862934639342	40.0	37.0	40.0	33.0	40.0
36-37	37.96626684136149	40.0	37.0	40.0	33.0	40.0
38-39	37.90503933661665	40.0	37.0	40.0	33.0	40.0
40-41	37.861522661043324	40.0	37.0	40.0	33.0	40.0
42-43	37.76019348535431	40.0	37.0	40.0	33.0	40.0
44-45	37.700953215148864	40.0	37.0	40.0	33.0	40.0
46-47	37.65120440031458	40.0	37.0	40.0	33.0	40.0
48-49	37.606230369524155	40.0	37.0	40.0	33.0	40.0
50-51	37.51902673470771	40.0	37.0	40.0	33.0	40.0
52-53	37.39999713940425	40.0	37.0	40.0	33.0	40.0
54-55	37.17225259963098	38.5	37.0	40.0	33.0	40.0
56-57	36.972374028277	37.0	37.0	40.0	33.0	40.0
58-59	36.880642538867846	37.0	37.0	40.0	33.0	40.0
60-61	36.728005486960754	37.0	37.0	40.0	33.0	40.0
62-63	36.53111930292337	37.0	37.0	40.0	33.0	40.0
64-65	36.36520203378615	37.0	37.0	40.0	33.0	40.0
66-67	36.13088796454026	37.0	37.0	40.0	33.0	40.0
68-69	35.90555090997066	37.0	35.0	38.5	33.0	40.0
70-71	35.64344362454227	37.0	33.0	37.0	33.0	40.0
72-73	35.44338222642252	37.0	33.0	37.0	33.0	40.0
74-75	35.28117116596012	37.0	33.0	37.0	33.0	40.0
76-77	33.46574551941829	35.0	33.0	37.0	27.0	37.0
78-79	34.77032563400577	37.0	33.0	37.0	33.0	37.0
80-81	34.87013251303987	37.0	33.0	37.0	33.0	37.0
82-83	34.77652744899717	37.0	33.0	37.0	33.0	37.0
84-85	34.635185557240035	37.0	33.0	37.0	33.0	37.0
86-87	34.52503333678918	37.0	33.0	37.0	33.0	37.0
88-89	34.46321777478123	37.0	33.0	37.0	33.0	37.0
90-91	34.38557088677822	37.0	33.0	37.0	30.0	37.0
92-93	34.31228663620675	37.0	33.0	37.0	27.0	37.0
94-95	34.30582545943552	37.0	33.0	37.0	27.0	37.0
96-97	34.32484419987371	37.0	33.0	37.0	30.0	37.0
98-99	34.332255574964165	37.0	33.0	37.0	30.0	37.0
100	34.33758457802979	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	10.0
20	67.0
21	334.0
22	1286.0
23	3646.0
24	8516.0
25	17208.0
26	29806.0
27	46910.0
28	69403.0
29	95988.0
30	129293.0
31	172066.0
32	227047.0
33	302550.0
34	414613.0
35	602654.0
36	1058655.0
37	2365546.0
38	2265865.0
39	43264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59180679562592	19.321417709000528	13.971226760337627	24.115548735035926
2	15.31356460547525	20.414822713807183	40.95056621304185	23.321046467675714
3	18.976631784495684	25.23952131257488	29.40519017269724	26.378656730232198
4	13.041608437413945	17.478387860357756	37.26435374154856	32.21564996067974
5	14.350794279471641	37.0193802994349	34.45370298580638	14.176122435287072
6	31.502096571644493	36.34930452079308	18.105159083802775	14.043439823759652
7	27.963752791471226	31.776895167229828	21.954252018115454	18.305100023183485
8	25.43434662099736	36.13025987274672	19.97566052246997	18.459732983785944
9	26.207664198217408	16.518851255084677	20.32943466286361	36.94404988383431
10-11	25.40663918085692	26.189791877968045	27.48561240908749	20.91795653208754
12-13	26.1466039501285	23.631188510990196	27.650733989869643	22.571473549011667
14-15	23.118632475106665	24.74447141740992	25.532919781815	26.603976325668416
16-17	22.829991970442265	27.971588835209975	26.00949695400058	23.188922240347186
18-19	22.855206080311618	26.966048096630708	27.74372610436337	22.435019718694306
20-21	23.8616033688662	26.034653712413956	27.719220493902103	22.384522424817735
22-23	23.581860728087076	26.03091492205251	27.511897100418587	22.875327249441824
24-25	23.310251369438305	26.207393572360328	27.696637434068737	22.78571762413263
26-27	23.119036491944637	26.265248290285065	27.720041858532607	22.89567335923769
28-29	23.137087577797118	26.28553535681023	27.670628591003698	22.90674847438895
30-31	23.47450402259309	26.00517641447534	27.59561987850283	22.924699684428735
32-33	23.236699434124972	26.216773684273363	27.4898249051794	23.056701976422268
34-35	23.38728742487592	26.295200339934755	27.472368311251323	22.845143923938
36-37	23.523754479428753	26.15592238258888	27.29562672394798	23.024696414034384
38-39	23.170443787598575	26.456653444878825	27.402875410946592	22.970027356576004
40-41	23.414151235831792	26.221618651772573	27.336636319696915	23.02759379269872
42-43	23.279433997339343	26.33265516239156	27.109280588279372	23.278630251989725
44-45	23.23998843121078	26.406048205765398	27.008142803236	23.345820559787818
46-47	23.447710617231074	26.380862550953033	26.744816516602576	23.426610315213317
48-49	23.462694856148254	26.65112066980927	26.51979988748837	23.3663845865541
50-51	23.424576967067456	26.68986480072595	26.546580255608742	23.338977976597853
52-53	23.699417762447826	26.56404466320489	26.447715889633947	23.288821684713334
54-55	23.68146960604859	26.50946106154676	26.386488880784892	23.42258045161976
56-57	23.663135405715522	26.794033099850452	26.162808998814153	23.38002249561988
58-59	23.46758717715886	26.766630739874525	26.42885607041886	23.336926012547757
60-61	23.59978764978749	26.717813428524874	26.31432619767409	23.368072724013548
62-63	23.564723991994796	26.803917342156268	26.331223880170928	23.300134785678008
64-65	23.645088502785303	26.689071998099863	26.29189348180922	23.373946017305617
66-67	23.704017243112396	26.75196092020917	26.090620880788478	23.45340095588995
68-69	23.642073138968968	26.675331211236397	26.225473172912068	23.457122476882567
70-71	23.627120169607927	26.543554295020506	26.20570406531959	23.62362147005198
72-73	23.630593972947167	26.60871211294892	26.06558624643919	23.695107667664725
74-75	23.477254216569115	26.669908805017943	26.15830223600191	23.694534742411033
76-77	23.608708198514254	26.673548406448933	26.070768858483483	23.64697453655333
78-79	23.473113978536812	26.673431359619727	26.175270941938084	23.678183719905384
80-81	23.572305210628468	26.659366940358776	26.144385244997704	23.623942604015056
82-83	23.669036833274674	26.561648393475302	26.305500674502863	23.46381409874716
84-85	23.66729114217423	26.580106601048893	26.22205044567984	23.53055181109704
86-87	23.439431494449035	26.732628119173068	26.24031007751938	23.58763030885852
88-89	23.654572661416516	26.582901623506576	26.09283044923482	23.669695265842087
90-91	23.624193972827975	26.676257997844832	26.10037859506847	23.599169434258723
92-93	23.66051817622445	26.673089820539918	26.215175758420166	23.451216244815463
94-95	23.407555178763108	26.81613601584209	26.28188777218348	23.49442103321132
96-97	23.47686213004042	26.863860657610438	26.31843115335798	23.340846058991158
98-99	24.78081613294452	28.816189471515326	26.35839040771754	20.044603987822622
100	23.562786178986094	29.837727964721022	20.22851522349669	26.370970632796194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1950.0
1	1623.5
2	1851.5
3	2551.0
4	3375.0
5	5469.0
6	7338.0
7	8641.0
8	9417.0
9	9230.5
10	8561.0
11	7852.5
12	8626.5
13	11583.0
14	18480.0
15	28393.0
16	36996.0
17	42324.0
18	43499.5
19	40828.0
20	35588.0
21	29281.5
22	24330.5
23	22568.0
24	24187.0
25	29270.5
26	37171.0
27	46135.0
28	56327.5
29	69402.0
30	83081.5
31	96632.0
32	112061.5
33	127977.5
34	143758.0
35	159106.5
36	176783.0
37	193779.0
38	207154.0
39	220473.5
40	234677.0
41	246858.0
42	255302.5
43	264192.0
44	274451.5
45	289800.0
46	306246.0
47	322228.5
48	333976.0
49	337664.0
50	341614.0
51	343907.5
52	332238.5
53	313765.0
54	301635.0
55	289375.0
56	274570.5
57	253986.5
58	229758.5
59	203142.0
60	173056.0
61	148220.0
62	123152.5
63	99750.5
64	81812.5
65	64504.0
66	51423.0
67	38098.0
68	25291.0
69	18568.5
70	13351.5
71	8339.5
72	5684.0
73	4054.5
74	3072.0
75	2216.0
76	1384.0
77	772.0
78	531.5
79	413.0
80	297.0
81	186.5
82	141.0
83	106.0
84	66.0
85	42.5
86	32.5
87	24.5
88	17.5
89	13.5
90	7.0
91	2.0
92	1.5
93	2.0
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.2731183978466985E-5
7	0.0
8	0.0
9	0.0
10-11	0.0016614195091899416
12-13	2.8645163951550713E-4
14-15	0.00723767809175848
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	475.0
22-23	995.0
24-25	2635.0
26-27	2879.0
28-29	4232.0
30-31	9262.0
32-33	11400.0
34-35	15963.0
36-37	23098.0
38-39	28847.0
40-41	31609.0
42-43	40537.0
44-45	49739.0
46-47	48490.0
48-49	42890.0
50-51	37020.0
52-53	36354.0
54-55	39610.0
56-57	42593.0
58-59	44418.0
60-61	44764.0
62-63	43840.0
64-65	47293.0
66-67	51405.0
68-69	52645.0
70-71	52646.0
72-73	50157.0
74-75	52048.0
76-77	51535.0
78-79	56590.0
80-81	59875.0
82-83	58887.0
84-85	59660.0
86-87	63580.0
88-89	70360.0
90-91	75506.0
92-93	81061.0
94-95	98613.0
96-97	289161.0
98-99	514194.0
100-101	5467732.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.22224223776534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.61995027866084	75.47993161705858
2	7.249501515560937	12.211385454932403
3	1.7391233391974157	4.394186014657082
4	0.6261656898703235	2.1094831365294335
5	0.2699884743913205	1.136951734579525
6	0.1547155544146578	0.7818294541116886
7	0.08818094693794694	0.519875795163431
8	0.05214125016047152	0.35131624012761226
9	0.039450275333246215	0.2990331580916881
>10	0.1531947186101766	2.1000841314760565
>50	0.005643090132198901	0.3170580623068936
>100	0.0018898296557550976	0.2559650757210959
>500	2.7518538595648895E-5	0.015829706751406804
>1k	2.7518536132478385E-5	0.027070418492962454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.092473591386794E-5	0.0	0.0	1.2731183978466985E-5
3	0.0	6.365591989233493E-5	0.0	0.0	1.2731183978466985E-5
4	0.0	1.0184947182773588E-4	0.0	0.0	1.2731183978466985E-5
5	0.0	1.0184947182773588E-4	0.0	0.0	1.2731183978466985E-5
6	1.2731183978466985E-5	1.0184947182773588E-4	0.0	0.0	1.2731183978466985E-5
7	1.2731183978466985E-5	1.0184947182773588E-4	0.0	0.0	1.2731183978466985E-5
8	1.2731183978466985E-5	1.1458065580620286E-4	0.0	0.0	2.546236795693397E-5
9	1.2731183978466985E-5	2.546236795693397E-4	0.0	0.0	2.546236795693397E-5
10-11	1.2731183978466985E-5	2.546236795693397E-4	0.0	0.0	2.546236795693397E-5
12-13	1.2731183978466985E-5	2.546236795693397E-4	0.0	6.365591989233492E-6	2.546236795693397E-5
14-15	2.546236795693397E-5	4.837849911817454E-4	0.0	1.2731183978466985E-5	3.182795994616746E-5
16-17	5.092473591386794E-5	4.965161751602124E-4	0.0	1.2731183978466985E-5	3.819355193540095E-5
18-19	5.092473591386794E-5	5.347097270956133E-4	0.0	1.2731183978466985E-5	6.365591989233493E-5
20-21	5.092473591386794E-5	5.920000549987148E-4	0.0	1.2731183978466985E-5	7.63871038708019E-5
22-23	5.092473591386794E-5	6.365591989233492E-4	0.0	1.2731183978466985E-5	8.27526958600354E-5
24-25	5.092473591386794E-5	6.620215668802832E-4	0.0	1.9096775967700476E-5	8.91182878492689E-5
26-27	5.092473591386794E-5	6.747527508587502E-4	0.0	3.182795994616746E-5	9.54838798385024E-5
28-29	5.092473591386794E-5	6.747527508587502E-4	0.0	9.548387983850238E-5	1.0184947182773588E-4
30-31	5.092473591386794E-5	8.14795774621887E-4	0.0	3.246451914509081E-4	1.0184947182773588E-4
32-33	5.092473591386794E-5	8.211613666111205E-4	0.0	9.357420224173232E-4	1.0184947182773588E-4
34-35	5.092473591386794E-5	0.0010248603102665922	0.0	0.0017123442451038094	1.0184947182773588E-4
36-37	5.092473591386794E-5	0.001228559253922064	0.0	0.0029790970509612743	1.0184947182773588E-4
38-39	5.092473591386794E-5	0.001279483989835932	0.0	0.0049778929355805916	1.1458065580620286E-4
40-41	5.092473591386794E-5	0.0013622366856959674	0.0	0.007460473811381652	1.1458065580620286E-4
42-43	5.092473591386794E-5	0.0014195270135990688	0.0	0.009904861135247314	1.1458065580620286E-4
44-45	5.092473591386794E-5	0.001591397997308373	0.0	0.012565678586746915	1.2094624779543635E-4
46-47	5.092473591386794E-5	0.001718709837093043	0.0	0.015684818661471325	1.2731183978466985E-4
48-49	5.092473591386794E-5	0.0017250754290822766	0.0	0.018765765184260336	1.2731183978466985E-4
50-51	5.092473591386794E-5	0.001973333516662383	0.0	0.021757593419200076	1.4004302376313684E-4
52-53	5.092473591386794E-5	0.0019924302926300833	0.0	0.02481307757403215	1.591397997308373E-4
54-55	5.092473591386794E-5	0.0019987958846193164	0.0	0.027607572457305656	1.655053917200708E-4
56-57	5.092473591386794E-5	0.0020115270685977836	0.0	0.030943142659664004	1.718709837093043E-4
58-59	5.092473591386794E-5	0.0020433550285439512	0.0	0.034756132261214864	1.782365756985378E-4
60-61	5.092473591386794E-5	0.002094279764457819	0.0	0.038594584230722664	1.9096775967700476E-4
62-63	6.365591989233493E-5	0.0021515700923609203	0.0	0.042433036200230465	1.9096775967700476E-4
64-65	6.365591989233493E-5	0.0023807314039733262	0.0	0.04613781073796436	1.9096775967700476E-4
66-67	7.63871038708019E-5	0.0023998281799410266	0.0	0.04991260678757981	1.9096775967700476E-4
68-69	7.63871038708019E-5	0.002469849691822595	0.0	0.05377015553305531	1.9733335166623826E-4
70-71	7.63871038708019E-5	0.0025398712037041633	0.0	0.05760860750256311	2.0369894365547176E-4
72-73	7.63871038708019E-5	0.002597161531607265	0.0	0.06082323145712602	2.418924955908727E-4
74-75	7.63871038708019E-5	0.002648086267521133	0.0	0.06315940371717471	2.609892715585732E-4
76-77	7.63871038708019E-5	0.0027626669233273356	0.0	0.06568017814491117	2.737204555370402E-4
78-79	7.63871038708019E-5	0.0028135916592412036	0.0	0.06711243634248872	2.800860475262737E-4
80-81	7.63871038708019E-5	0.0028135916592412036	0.0	0.06763441488560586	2.8645163951550713E-4
82-83	7.63871038708019E-5	0.0028135916592412036	0.0	0.0680036192209814	2.9281723150474063E-4
84-85	7.63871038708019E-5	0.002819957251230437	0.0	0.068156393428723	2.9281723150474063E-4
86-87	7.63871038708019E-5	0.0028963443551012387	0.0	0.0682327805325938	2.9281723150474063E-4
88	8.91182878492689E-5	0.0030427529708536094	0.0	0.0682518773085615	2.9281723150474063E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10630	0.0	53.984623	1
GTATCAA	19395	0.0	38.924274	1
TCAACGC	23820	0.0	31.160872	4
ATCAACG	24295	0.0	30.533632	3
CAACGCA	24600	0.0	30.192162	5
TATCAAC	24725	0.0	30.108753	2
AACGCAG	25155	0.0	29.543417	6
ACGCAGA	28965	0.0	25.581987	7
GTGGTAT	5085	0.0	24.514523	1
TGGTATC	4875	0.0	24.045273	2
CGCAGAG	31625	0.0	23.471764	8
GTACATG	28940	0.0	22.806583	1
TACATGG	29260	0.0	22.317987	2
GCAGAGT	34920	0.0	21.15962	9
ACATGGG	29995	0.0	21.041998	3
GAGTACT	17865	0.0	20.49523	12-13
TATATAC	7825	0.0	19.39611	3
CAGAGTA	33695	0.0	19.384151	10-11
CATGGGG	22895	0.0	18.492867	4
GTACTTT	19755	0.0	18.457434	14-15
>>END_MODULE