FastQCFastQC Report
Fri 27 May 2016
SRR2139203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11497457
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA223050.057.114581
GTATCAA414150.039.2145461
GTGGTAT84350.031.8440461
TATCAAC531350.030.2661972
TCAACGC530500.030.1852844
ATCAACG530250.030.1833323
TGGTATC85100.029.496152
CAACGCA543650.029.440265
AACGCAG558150.028.6370036
ACGCAGA631700.025.2756827
CGCAGAG648200.024.6392298
GTACATG521350.022.3284931
GCAGAGT720800.022.1009799
TACATGG525100.021.727782
GAGTACT416050.021.42377912-13
ACATGGG537950.020.7461473
CAGAGTA704500.020.2822610-11
GTACTTT456350.019.49470914-15
CATGGGG365300.019.4865844
AGAGTAC669500.019.25607110-11
Produced by FastQC (version 0.11.5)