##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11497457
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.588922750483	33.0	33.0	33.0	33.0	33.0
2	32.58719193296396	33.0	33.0	33.0	33.0	33.0
3	32.5700381397382	33.0	33.0	33.0	33.0	33.0
4	36.47939644392669	37.0	37.0	37.0	37.0	37.0
5	36.5116914983896	37.0	37.0	37.0	37.0	37.0
6	36.51391990420142	37.0	37.0	37.0	37.0	37.0
7	36.49018082868238	37.0	37.0	37.0	37.0	37.0
8	36.47560795400235	37.0	37.0	37.0	37.0	37.0
9	36.50504759443762	37.0	37.0	37.0	37.0	37.0
10-11	36.462616559470504	37.0	37.0	37.0	37.0	37.0
12-13	36.39131522735853	37.0	37.0	37.0	37.0	37.0
14-15	38.403768111505	40.0	37.0	40.0	37.0	40.0
16-17	38.36527781752086	40.0	37.0	40.0	37.0	40.0
18-19	38.39875369831781	40.0	37.0	40.0	37.0	40.0
20-21	38.40272694330844	40.0	37.0	40.0	37.0	40.0
22-23	38.3990275878394	40.0	37.0	40.0	37.0	40.0
24-25	38.39814679657498	40.0	37.0	40.0	37.0	40.0
26-27	38.24817984424301	40.0	37.0	40.0	37.0	40.0
28-29	38.16764476745499	40.0	37.0	40.0	37.0	40.0
30-31	38.110994586220436	40.0	37.0	40.0	35.0	40.0
32-33	38.023258011537024	40.0	37.0	40.0	33.0	40.0
34-35	37.98245605703448	40.0	37.0	40.0	33.0	40.0
36-37	37.9401642548246	40.0	37.0	40.0	33.0	40.0
38-39	37.88871483306402	40.0	37.0	40.0	33.0	40.0
40-41	37.8520031466443	40.0	37.0	40.0	33.0	40.0
42-43	37.75146278837765	40.0	37.0	40.0	33.0	40.0
44-45	37.69434694824706	40.0	37.0	40.0	33.0	40.0
46-47	37.64851625905186	40.0	37.0	40.0	33.0	40.0
48-49	37.61394830849418	40.0	37.0	40.0	33.0	40.0
50-51	37.55160122611014	40.0	37.0	40.0	33.0	40.0
52-53	37.45128039040662	40.0	37.0	40.0	33.0	40.0
54-55	37.24100779008366	40.0	37.0	40.0	33.0	40.0
56-57	37.05705146888331	37.0	37.0	40.0	33.0	40.0
58-59	36.98947068399055	37.0	37.0	40.0	33.0	40.0
60-61	36.85085432374794	37.0	37.0	40.0	33.0	40.0
62-63	36.660422368341344	37.0	37.0	40.0	33.0	40.0
64-65	36.50611237009299	37.0	37.0	40.0	33.0	40.0
66-67	36.28375280489767	37.0	37.0	40.0	33.0	40.0
68-69	36.06145890869292	37.0	37.0	40.0	33.0	40.0
70-71	35.8008537593625	37.0	35.0	37.0	33.0	40.0
72-73	35.599476315218006	37.0	33.0	37.0	33.0	40.0
74-75	35.43031738883874	37.0	33.0	37.0	33.0	40.0
76-77	33.63264503643644	35.0	33.0	37.0	27.0	37.0
78-79	34.901355018988916	37.0	33.0	37.0	33.0	37.0
80-81	34.98911233044633	37.0	33.0	37.0	33.0	37.0
82-83	34.8876799035234	37.0	33.0	37.0	33.0	37.0
84-85	34.741982988366374	37.0	33.0	37.0	33.0	37.0
86-87	34.62731689195855	37.0	33.0	37.0	33.0	37.0
88-89	34.559701924798006	37.0	33.0	37.0	33.0	37.0
90-91	34.48147163986005	37.0	33.0	37.0	33.0	37.0
92-93	34.410883151588095	37.0	33.0	37.0	33.0	37.0
94-95	34.4028564698297	37.0	33.0	37.0	33.0	37.0
96-97	34.41053434966553	37.0	33.0	37.0	33.0	37.0
98-99	34.41575986103731	37.0	33.0	37.0	33.0	37.0
100	34.426904247596745	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	4.0
18	6.0
19	18.0
20	119.0
21	523.0
22	1804.0
23	5064.0
24	11842.0
25	23106.0
26	40341.0
27	64612.0
28	96101.0
29	134722.0
30	183184.0
31	245849.0
32	326148.0
33	436792.0
34	597014.0
35	858107.0
36	1474947.0
37	3385639.0
38	3528242.0
39	83272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71273204152883	19.594167649420218	14.462311100619901	23.230789208431048
2	15.02657500697763	20.70413483607723	41.703291432183654	22.565998724761485
3	18.647645301043525	26.166186140117766	30.09815126945028	25.088017289388425
4	12.676681460952627	17.83981449115226	38.17661592472144	31.30688812317367
5	13.552631681944973	37.624780853714	34.80018233597221	14.02240512836882
6	30.14496686440608	37.33547989533336	18.424607880613582	14.094945359646982
7	26.953490671893793	32.83974012688197	22.318256985001117	17.88851221622312
8	25.05346182203595	36.37374769046755	20.38478595745129	18.18800453004521
9	26.31721084062328	16.62197997348457	20.558607003270378	36.50220218262177
10-11	24.907282401973756	26.71160947834783	27.811204530324478	20.569903589353935
12-13	25.962791333610326	24.35406211249489	28.14592288415088	21.537223669743906
14-15	22.910307012603432	25.322540198522542	26.420114104751924	25.347038684122104
16-17	21.873554299876922	28.60262491088247	26.974560548476067	22.549260240764546
18-19	21.870723239060602	27.282806972011286	29.38145800414822	21.465011784779886
20-21	23.06318652525037	26.241095704062907	29.392667850093574	21.30304992059315
22-23	22.750842857784686	26.28578951582644	29.21649944483619	21.746868181552678
24-25	22.286925150660522	26.51942327678425	29.363965283152165	21.82968628940306
26-27	22.120435925987596	26.681426508928542	29.324236730438457	21.873900834645404
28-29	22.067101769716906	26.703332820392106	29.36263264081042	21.866932769080567
30-31	22.39417481779416	26.58385761847384	29.190854174525764	21.83111338920623
32-33	22.186531903999327	26.75594830187521	29.10301546779202	21.954504326333442
34-35	22.31166877558733	26.770561276295794	29.064504791062824	21.853265157054054
36-37	22.381314245275618	26.734133804140324	28.92756276652284	21.95698918406121
38-39	22.173136938902395	26.94073998765003	28.963666877437355	21.922456196010216
40-41	22.4085478013691	26.7931375192785	28.788997351405964	22.009317327946434
42-43	22.34719680729545	26.916727542063345	28.492526844421935	22.243548806219273
44-45	22.422790779460883	27.06332206182674	28.227982448289957	22.285904710422418
46-47	22.590232772463768	27.103179136734685	27.721463681941383	22.585124408860164
48-49	22.538767035565854	27.345884485484117	27.568948509261855	22.54639996968818
50-51	22.621130948987414	27.49587636523344	27.321878389670246	22.561114296108897
52-53	22.804133381774474	27.38723437156378	27.219180350057716	22.58945189660403
54-55	22.785042635742833	27.285215352224952	27.231265709495787	22.698476302536427
56-57	22.73863410105609	27.51295690751559	27.114639199778868	22.633769791649453
58-59	22.679665582032737	27.485428951071782	27.203423181120872	22.631482285774602
60-61	22.78422895139201	27.509458446324015	27.13123524409284	22.57507735819114
62-63	22.715907459627775	27.58742774298734	27.119319674470667	22.57734512291422
64-65	22.755206795951636	27.481359207778873	27.044279646956525	22.719154349312966
66-67	22.80697270804789	27.512846495236076	26.923768739190752	22.756412057525285
68-69	22.76794916877857	27.485706134268533	27.033203015826494	22.7131416811264
70-71	22.77020903156387	27.297396604761033	27.081589803628546	22.85080456004655
72-73	22.819059043785305	27.362983634780296	26.931279127255202	22.88667819417919
74-75	22.649750810919667	27.491332526207252	26.948246622474077	22.91067004039901
76-77	22.837320664135444	27.370053036323384	26.909127774452955	22.883498525088218
78-79	22.791216059135806	27.34751567015258	26.88353255843427	22.97773571227734
80-81	22.776217382164425	27.401842812801824	26.894030707710197	22.927909097323557
82-83	22.864287232444777	27.289863840240674	27.012252882749337	22.833596044565205
84-85	22.925546501950144	27.244667522898013	26.94353395815023	22.88625201700161
86-87	22.769971989084766	27.280841560148065	27.061834517566545	22.88735193320062
88-89	22.86201668012751	27.214036535577428	26.98057198895536	22.943374795339697
90-91	22.92716069126073	27.18194612077588	26.985632111381857	22.90526107658153
92-93	22.96955725318074	27.096253247654932	27.071229394001584	22.862960105162745
94-95	22.804515376479998	27.117862628969814	27.10081425137779	22.9768077431724
96-97	22.905587783630978	27.24908846537279	27.138126075490526	22.707197675505704
98-99	24.047787923904885	29.141783269016873	27.318794549713655	19.49163425736459
100	22.930901071970986	30.147137063685214	21.15487081198241	25.76709105236139
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2930.0
1	2606.5
2	3777.0
3	5627.5
4	7856.5
5	13396.0
6	18034.5
7	21049.5
8	22996.5
9	22704.0
10	20962.5
11	19123.5
12	20968.5
13	28459.5
14	46632.5
15	72261.0
16	93972.5
17	107320.5
18	109543.5
19	100507.5
20	85181.0
21	68152.0
22	54160.0
23	47843.0
24	49550.5
25	57961.5
26	71680.0
27	87985.0
28	105819.5
29	127254.0
30	150508.0
31	174144.5
32	200850.5
33	227967.0
34	255157.5
35	280698.0
36	307815.0
37	335392.5
38	355846.0
39	372093.5
40	388699.0
41	401609.0
42	410537.5
43	423041.5
44	435029.0
45	446368.5
46	456290.0
47	464616.5
48	471486.0
49	471656.5
50	468019.0
51	461486.5
52	445436.5
53	422337.5
54	400886.5
55	378278.0
56	352291.0
57	323017.0
58	290804.0
59	254229.0
60	214259.0
61	177498.5
62	142504.0
63	110723.5
64	84769.5
65	63431.5
66	48077.5
67	36718.5
68	27098.0
69	19160.0
70	13363.5
71	9704.0
72	6933.5
73	4832.0
74	3408.5
75	2396.5
76	1545.0
77	930.5
78	643.0
79	474.5
80	326.5
81	224.5
82	162.5
83	111.5
84	76.0
85	52.5
86	39.5
87	33.0
88	26.5
89	17.0
90	9.5
91	4.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.739515094511769E-5
7	0.0
8	0.0
9	0.0
10-11	0.0015568660095880331
12-13	2.4353211323164766E-4
14-15	0.007201592491278724
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	206.0
20-21	753.0
22-23	1550.0
24-25	4739.0
26-27	5527.0
28-29	8956.0
30-31	18891.0
32-33	23421.0
34-35	33061.0
36-37	45133.0
38-39	58635.0
40-41	66531.0
42-43	86303.0
44-45	106736.0
46-47	101216.0
48-49	86121.0
50-51	72178.0
52-53	67893.0
54-55	70755.0
56-57	75808.0
58-59	77017.0
60-61	77344.0
62-63	75331.0
64-65	79252.0
66-67	88107.0
68-69	90686.0
70-71	91745.0
72-73	87350.0
74-75	89766.0
76-77	88905.0
78-79	97604.0
80-81	100548.0
82-83	98016.0
84-85	99096.0
86-87	104809.0
88-89	114061.0
90-91	120636.0
92-93	127695.0
94-95	149181.0
96-97	395563.0
98-99	707421.0
100-101	7602911.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.09111044202332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.04198875701249	74.87639710543337
2	7.678896041420205	12.914537901837644
3	1.8325948669799557	4.623148120640895
4	0.664302250969008	2.234476558124782
5	0.3068477562791804	1.2901584281079803
6	0.1547278864253281	0.7806743871511863
7	0.09112803679535675	0.5364140464566176
8	0.056710450830403485	0.38150758271971136
9	0.04048584913610848	0.3064050009939233
>10	0.12833707731980862	1.6692893758736256
>50	0.0029174017106903052	0.1666289888524618
>100	9.404661389658405E-4	0.1196832323100662
>500	8.79720749048101E-5	0.05249348519535313
>1k	3.5186907571696254E-5	0.04818578630237035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.697575472558846E-6	0.0	0.0	0.0
3	0.0	8.697575472558846E-6	0.0	8.697575472558846E-6	0.0
4	0.0	6.0883028307911916E-5	0.0	8.697575472558846E-6	0.0
5	0.0	6.0883028307911916E-5	0.0	8.697575472558846E-6	0.0
6	0.0	6.0883028307911916E-5	0.0	8.697575472558846E-6	0.0
7	0.0	6.0883028307911916E-5	0.0	8.697575472558846E-6	0.0
8	0.0	7.82781792530296E-5	0.0	8.697575472558846E-6	0.0
9	0.0	2.2613696228652996E-4	0.0	4.3487877362794224E-5	0.0
10-11	0.0	2.2613696228652996E-4	0.0	8.697575472558845E-5	0.0
12-13	0.0	2.2613696228652996E-4	0.0	1.4350999529722094E-4	0.0
14-15	0.0	3.1311271701211845E-4	0.0	1.4785878303350035E-4	0.0
16-17	1.3046363208838268E-5	3.218102924846773E-4	0.0	1.565563585060592E-4	0.0
18-19	1.739515094511769E-5	3.261590802209567E-4	0.0	2.0004423586885344E-4	0.0
20-21	1.739515094511769E-5	3.3485665569351556E-4	0.0	2.522296887042065E-4	0.0
22-23	2.1743938681397112E-5	3.39205443429795E-4	0.0	2.7832241512188306E-4	0.0
24-25	2.6092726417676533E-5	3.783445330563098E-4	0.0	3.522518066386332E-4	0.0
26-27	2.6092726417676533E-5	3.91390896265148E-4	0.0	6.697133113870311E-4	0.0
28-29	2.6092726417676533E-5	3.91390896265148E-4	0.0	0.0013394266227740623	4.348787736279423E-6
30-31	2.6092726417676533E-5	4.479251368367805E-4	0.0	0.003061546566340713	8.697575472558846E-6
32-33	2.6092726417676533E-5	4.5227392457305993E-4	0.0	0.0066797379629251925	8.697575472558846E-6
34-35	2.6092726417676533E-5	5.262033160898102E-4	0.0	0.011359033567161851	8.697575472558846E-6
36-37	2.6092726417676533E-5	6.044814953428397E-4	0.0	0.01769086851118469	8.697575472558846E-6
38-39	2.6092726417676533E-5	6.392717972330751E-4	0.0	0.02718427213948267	8.697575472558846E-6
40-41	2.6092726417676533E-5	7.566890661126196E-4	0.0	0.03635151668755969	8.697575472558846E-6
42-43	2.6092726417676533E-5	7.958281557391343E-4	0.0	0.04477511853273293	8.697575472558846E-6
44-45	2.6092726417676533E-5	8.915014859372816E-4	0.0	0.05302911765619128	8.697575472558846E-6
46-47	2.6092726417676533E-5	9.480357265089141E-4	0.0	0.060478591048437925	8.697575472558846E-6
48-49	2.6092726417676533E-5	9.56733301981473E-4	0.0	0.06688000659624124	8.697575472558846E-6
50-51	3.479030189023538E-5	0.0010698017831247378	0.0	0.07240731580905238	8.697575472558846E-6
52-53	3.913908962651481E-5	0.0010871969340698557	0.0	0.07765195381900536	1.3046363208838268E-5
54-55	4.3487877362794224E-5	0.0010958945095424144	0.0	0.08249215456948436	1.739515094511769E-5
56-57	4.783666509907364E-5	0.001121987235960091	0.0	0.08679745442840099	1.739515094511769E-5
58-59	5.218545283535307E-5	0.0011437311746414882	0.0	0.09136368155149438	1.739515094511769E-5
60-61	5.218545283535307E-5	0.0011915678397405617	0.0	0.09615169684913803	1.739515094511769E-5
62-63	5.218545283535307E-5	0.0012263581416307972	0.0	0.10060485549108816	2.6092726417676533E-5
64-65	5.218545283535307E-5	0.0013916120756094152	0.0	0.10481883080754292	2.6092726417676533E-5
66-67	5.653424057163249E-5	0.0014090072265545328	0.0	0.10931547732685584	2.6092726417676533E-5
68-69	6.0883028307911916E-5	0.001461192679389886	0.0	0.11412958535091716	2.6092726417676533E-5
70-71	6.0883028307911916E-5	0.0015003317690164006	0.0	0.11816526037018446	2.6092726417676533E-5
72-73	6.0883028307911916E-5	0.0015090293444889598	0.0	0.12118766784689866	2.6092726417676533E-5
74-75	6.0883028307911916E-5	0.0015307732831703568	0.0	0.12378389412545748	2.6092726417676533E-5
76-77	7.392939151675018E-5	0.0015873075237419893	0.0	0.1266106061540391	2.6092726417676533E-5
78-79	7.82781792530296E-5	0.0016177490378959452	0.0	0.12843709700327646	2.6092726417676533E-5
80-81	7.82781792530296E-5	0.0016220978256322246	0.0	0.12901983455993793	2.6092726417676533E-5
82-83	7.82781792530296E-5	0.001626446613368504	0.0	0.12949820121092864	4.3487877362794224E-5
84-85	7.82781792530296E-5	0.001626446613368504	0.0	0.1297460821118966	4.3487877362794224E-5
86-87	8.697575472558845E-5	0.0016742832784675777	4.348787736279423E-6	0.12984175544209473	4.3487877362794224E-5
88	8.697575472558845E-5	0.001739515094511769	8.697575472558846E-6	0.129846104229831	4.3487877362794224E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22305	0.0	57.11458	1
GTATCAA	41415	0.0	39.214546	1
GTGGTAT	8435	0.0	31.844046	1
TATCAAC	53135	0.0	30.266197	2
TCAACGC	53050	0.0	30.185284	4
ATCAACG	53025	0.0	30.183332	3
TGGTATC	8510	0.0	29.49615	2
CAACGCA	54365	0.0	29.44026	5
AACGCAG	55815	0.0	28.637003	6
ACGCAGA	63170	0.0	25.275682	7
CGCAGAG	64820	0.0	24.639229	8
GTACATG	52135	0.0	22.328493	1
GCAGAGT	72080	0.0	22.100979	9
TACATGG	52510	0.0	21.72778	2
GAGTACT	41605	0.0	21.423779	12-13
ACATGGG	53795	0.0	20.746147	3
CAGAGTA	70450	0.0	20.28226	10-11
GTACTTT	45635	0.0	19.494709	14-15
CATGGGG	36530	0.0	19.486584	4
AGAGTAC	66950	0.0	19.256071	10-11
>>END_MODULE