FastQCFastQC Report
Fri 27 May 2016
SRR2139202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6827097
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81400.051.3945541
GTATCAA143600.036.0175251
TCAACGC173800.029.0998694
ATCAACG176400.028.670963
CAACGCA180100.027.9877075
TATCAAC184000.027.6020222
AACGCAG185800.027.106266
CGAGACC47950.025.17477686-87
GAGACCG47400.024.94934788-89
GACCGAG47700.024.8775290-91
AGACCGA49350.024.66487988-89
ACCGAGG50850.024.1532190-91
ACGCAGA212850.023.7611357
CACGAGA51400.023.67834584-85
ACGAGAC50200.023.42696486-87
TCTCCGA50100.022.72860774-75
CCCACGA52200.022.68673382-83
GCCCACG51550.021.96474882-83
CGCAGAG231950.021.7679318
GTGGTAT36650.021.4171831
Produced by FastQC (version 0.11.5)