##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6827097
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00317045444059	31.0	31.0	34.0	30.0	34.0
2	32.183962964053386	33.0	31.0	34.0	30.0	34.0
3	32.19703059733881	34.0	31.0	34.0	30.0	34.0
4	35.95809170427782	37.0	35.0	37.0	35.0	37.0
5	35.819680312144385	37.0	35.0	37.0	35.0	37.0
6	35.84033784784367	37.0	35.0	37.0	35.0	37.0
7	35.782967782646125	37.0	35.0	37.0	33.0	37.0
8	35.772684202377675	37.0	35.0	37.0	33.0	37.0
9	37.54695839241774	39.0	37.0	39.0	35.0	39.0
10-11	37.42054221582028	39.0	37.0	39.0	34.0	39.0
12-13	37.394445032786265	39.0	37.0	39.0	34.0	39.0
14-15	38.626818763524234	40.0	38.0	41.0	34.0	41.0
16-17	38.58655758076969	40.0	38.0	41.0	34.0	41.0
18-19	38.604685271060305	40.0	38.0	41.0	34.0	41.0
20-21	38.58449637914734	40.0	38.0	41.0	34.0	41.0
22-23	38.49789858379733	40.0	38.0	41.0	34.0	41.0
24-25	38.43604455952327	40.0	38.0	41.0	34.0	41.0
26-27	38.26150605380727	40.0	38.0	41.0	33.5	41.0
28-29	38.15142151862369	40.0	38.0	41.0	33.0	41.0
30-31	37.996862207064325	40.0	37.0	41.0	33.0	41.0
32-33	37.81756641606299	40.0	37.0	41.0	33.0	41.0
34-35	37.908011532961304	40.0	37.0	41.0	33.0	41.0
36-37	37.87819949940017	40.0	37.0	41.0	33.0	41.0
38-39	37.83419006352854	40.0	37.0	41.0	33.0	41.0
40-41	37.7516334331785	40.0	37.0	41.0	33.0	41.0
42-43	37.643256373835364	40.0	37.0	41.0	32.0	41.0
44-45	37.692044025978724	40.0	37.0	41.0	32.0	41.0
46-47	37.68237275752934	40.0	37.0	41.0	32.0	41.0
48-49	37.61625675991212	40.0	37.0	41.0	32.0	41.0
50-51	37.51192143713102	40.0	36.0	41.0	32.0	41.0
52-53	37.30809728236191	39.0	36.0	41.0	31.0	41.0
54-55	37.16952141795453	39.0	35.5	41.0	31.0	41.0
56-57	36.98918766251924	39.0	35.0	41.0	31.0	41.0
58-59	36.836910316372915	39.0	35.0	40.0	31.0	41.0
60-61	36.60793611171733	38.0	35.0	40.0	31.0	41.0
62-63	36.383319555606704	38.0	35.0	40.0	30.0	41.0
64-65	36.07147970923325	37.5	34.5	40.0	30.0	41.0
66-67	35.78321315514985	37.0	34.0	40.0	29.5	41.0
68-69	35.47748375766561	36.5	34.0	39.0	29.0	41.0
70-71	35.19018911126852	36.0	34.0	39.0	29.0	41.0
72-73	34.91517187803148	35.5	34.0	39.0	29.0	40.0
74-75	34.56041905558176	35.0	33.5	38.0	29.0	40.0
76-77	33.299481508059024	34.5	31.5	36.0	27.0	39.0
78-79	33.99663457617338	35.0	33.0	37.0	29.0	39.0
80-81	33.93141840024704	35.0	33.0	36.0	29.0	39.0
82-83	33.6658703395277	35.0	33.0	36.0	29.0	37.5
84-85	33.50571382989943	35.0	33.0	35.5	29.0	37.0
86-87	33.29162824400517	35.0	33.0	35.0	29.0	37.0
88-89	33.206089710005415	35.0	33.0	35.0	29.0	36.0
90-91	33.08353535391427	35.0	33.0	35.0	29.0	36.0
92-93	32.963856371691264	35.0	33.0	35.0	29.0	36.0
94-95	32.93928512351441	35.0	33.0	35.0	29.0	35.5
96-97	33.01633949160511	34.0	33.0	35.0	29.0	35.0
98-99	33.1665351353665	34.0	33.0	35.0	29.0	35.0
100	31.958592979413535	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	5.0
20	44.0
21	224.0
22	1130.0
23	4213.0
24	11321.0
25	24159.0
26	44087.0
27	70633.0
28	99771.0
29	132018.0
30	166049.0
31	203436.0
32	249022.0
33	311178.0
34	406162.0
35	557941.0
36	854232.0
37	1374668.0
38	1572278.0
39	719058.0
40	25464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61836282683547	20.114098276324476	14.346947172421896	23.920591724418152
2	15.032348302653384	21.329768714286615	40.65016214065803	22.987720842401977
3	18.210082557784077	26.16333120797903	30.477155956624024	25.149430277612872
4	12.812576121300165	18.32617875504039	38.4293646333134	30.431880490346042
5	13.489774643600347	37.624498377568095	35.42870417689979	13.457022801931773
6	30.107804239488615	37.8041794338062	18.71417675770536	13.373839568999827
7	26.74095592899881	32.44220786668184	23.10339226174756	17.713443942571786
8	24.82327407974429	36.241509385321464	20.873513295621844	18.061703239312404
9	25.78536675251575	16.637203191927696	21.026887416423115	36.55054263913344
10-11	24.460140232371096	26.573183301775266	28.653005809057642	20.313670656796
12-13	25.344209309492644	24.185202610304586	28.54817040803293	21.922417672169836
14-15	22.369603068478447	25.408134672760617	26.3472673670815	25.874994891679435
16-17	22.042379052614177	28.629227195990175	26.851887318179497	22.47650643321615
18-19	22.097961170388576	27.762559157741844	28.4546513645381	21.68482830733148
20-21	23.056035463612425	26.869109133179485	28.36194727903301	21.712908124175083
22-23	22.79705411483977	26.776498375019486	28.164873699224486	22.26157381091626
24-25	22.52370034737114	26.954979193923705	28.322213656915356	22.199106801789796
26-27	22.431069483356254	27.04227804805329	28.24960558049185	22.27704688809861
28-29	22.461447734697614	27.08372305477273	28.258427497732804	22.196401712796852
30-31	22.774711983808103	26.825724968304087	28.1873975434658	22.21216550442201
32-33	22.553444989657752	26.992774922221635	28.05125668913066	22.402523398989953
34-35	22.684333272209596	27.073686820405783	28.049339771372615	22.192640136012002
36-37	22.766922542710585	26.95850226649366	27.923286379508816	22.351288811286935
38-39	22.443293823150334	27.258360836493402	28.01183815476767	22.28650718558859
40-41	22.682934747021537	27.031194040581784	27.952215075493974	22.3336561369027
42-43	22.500843740217537	27.174753148900386	27.738268198953755	22.586134911928315
44-45	22.556453144183443	27.21596644247169	27.610570109796207	22.617010303548664
46-47	22.698790203218934	27.179767495975486	27.429660600929978	22.691781699875598
48-49	22.74032201401066	27.37507629239511	27.269736561487214	22.61486513210702
50-51	22.682323363548296	27.416929712084738	27.25755383169275	22.643193092674217
52-53	22.886967615302943	27.307516425250338	27.145020928071162	22.66049503137556
54-55	22.93183437340465	27.230010057832537	27.138352979124917	22.699802589637894
56-57	22.886819518630574	27.393476826883102	27.03192646667697	22.687777187809353
58-59	22.684885056212067	27.305440706997413	27.16185763389917	22.84781660289135
60-61	22.90806604595217	27.26548310497008	27.22194876918957	22.604502079888185
62-63	22.73307509391814	27.309170063519954	27.284348373766036	22.67340646879587
64-65	22.782744916466648	27.223637158637416	27.20915571008305	22.784462214812887
66-67	22.80297904311151	27.376614041483695	27.159280320742063	22.661126594662733
68-69	22.751291569084582	27.46871486823903	27.101368145957345	22.678625416719044
70-71	22.75073766391113	27.298525012507103	26.92267376366848	23.028063559913285
72-73	22.74825963857431	27.3175014864817	26.86195373731934	23.072285137624647
74-75	22.652037894222588	27.390057917791378	27.07703220876882	22.88087197921721
76-77	22.790350993928595	27.169734204465612	27.028869277839675	23.011045523766114
78-79	22.64598469872973	27.211769317849278	26.852112624816836	23.290133358604155
80-81	22.8971459246346	27.35056813999458	26.89137924534061	22.860906690030216
82-83	23.00330033602959	27.073330181503945	27.056786711150828	22.866582771315638
84-85	22.79159524259362	27.091961108529194	26.980679759509457	23.13576388936773
86-87	22.63946888381596	27.421579756116433	26.9922513432982	22.946700016769405
88-89	23.05566473084172	27.22634423834731	26.875665771245743	22.84232525956523
90-91	23.02562953624991	27.232806512289187	26.964894667121403	22.7766692843395
92-93	22.815395817431856	27.03048593609998	27.080371835890908	23.073746410577257
94-95	22.798571299518443	27.36878139450386	27.15837665347001	22.67427065250768
96-97	23.021536481471415	27.259965911567658	27.24448699100124	22.47401061595969
98-99	23.82280384877885	29.374122536683455	27.524190871458277	19.278882743079418
100	22.753961265066845	30.466387168596746	21.226103107293035	25.553548459043373
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1612.0
1	1314.5
2	1566.0
3	2213.0
4	2816.5
5	4416.5
6	5971.0
7	7369.0
8	8596.5
9	8940.0
10	8232.5
11	7220.5
12	7576.0
13	9939.5
14	15421.0
15	23379.5
16	30317.0
17	34655.5
18	36221.0
19	34963.5
20	32013.5
21	28990.0
22	27343.0
23	28339.0
24	32955.5
25	41059.0
26	52261.0
27	64389.0
28	76596.0
29	90739.5
30	104572.0
31	117477.5
32	131617.0
33	145966.5
34	159920.5
35	173922.0
36	189560.0
37	202776.0
38	212219.5
39	222912.5
40	234129.0
41	242783.5
42	248301.0
43	253590.5
44	259680.0
45	268704.0
46	277687.5
47	286260.5
48	290941.5
49	288663.5
50	286805.5
51	284112.5
52	271639.0
53	252973.5
54	238380.0
55	226245.0
56	212882.0
57	195679.5
58	176007.0
59	155213.5
60	133110.0
61	113556.0
62	94556.0
63	77102.0
64	63354.0
65	50505.5
66	40215.0
67	30188.0
68	20792.0
69	15551.5
70	11577.0
71	7733.0
72	5443.5
73	3937.5
74	2915.5
75	2068.0
76	1315.0
77	770.0
78	533.5
79	388.0
80	263.0
81	173.0
82	123.5
83	82.5
84	53.5
85	35.5
86	24.0
87	19.5
88	14.5
89	9.0
90	6.5
91	5.5
92	4.0
93	2.5
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.4647514163047632E-5
14-15	0.0
16-17	0.0010033547201687629
18-19	0.0017064353999950493
20-21	0.0
22-23	0.0033034709811316136
24-25	2.71073888514636E-4
26-27	0.0016563659272922785
28-29	0.0052061996011464485
30-31	0.0015710060987337692
32-33	0.005333803787088972
34-35	0.011340426621389564
36-37	5.334118041365197E-4
38-39	3.725031578955211E-5
40-41	0.002803866607020777
42-43	0.005902624954683012
44-45	0.001834801059982083
46-47	0.0018904158223193557
48-49	0.002722023050959761
50-51	0.01112073900517091
52-53	0.006531224254745164
54-55	0.010150459330109364
56-57	0.01855175808507457
58-59	0.012596669775563429
60-61	0.01631257323826237
62-63	0.015252414365548658
64-65	0.010872288583725498
66-67	0.01324247556121553
68-69	0.009058243241275148
70-71	0.00882226054560024
72-73	0.0064905774438936865
74-75	0.010396914591822085
76-77	0.012191532932104045
78-79	0.00727548001753923
80-81	0.004039384900420186
82-83	0.002171226560405567
84-85	9.193765597223335E-6
86-87	0.003947777238475819
88-89	8.02483052502029E-4
90-91	7.289021199542709E-4
92-93	0.0016494967717200903
94-95	0.0011155061833404132
96-97	4.2996816699963604E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	157.0
20-21	547.0
22-23	1116.0
24-25	2409.0
26-27	2854.0
28-29	5851.0
30-31	13798.0
32-33	18087.0
34-35	25051.0
36-37	35822.0
38-39	41322.0
40-41	43866.0
42-43	53752.0
44-45	60639.0
46-47	60191.0
48-49	55555.0
50-51	48315.0
52-53	45363.0
54-55	49001.0
56-57	52766.0
58-59	32978.0
60-61	54013.0
62-63	53233.0
64-65	55958.0
66-67	59793.0
68-69	61802.0
70-71	61958.0
72-73	59459.0
74-75	60492.0
76-77	59546.0
78-79	64655.0
80-81	66620.0
82-83	65081.0
84-85	67172.0
86-87	72484.0
88-89	80378.0
90-91	88190.0
92-93	99411.0
94-95	117791.0
96-97	293176.0
98-99	475653.0
100-101	4160792.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.00653980199718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.48328820430677	79.59644356365597
2	6.0606465587958605	10.546317720874185
3	1.3606754579687983	3.55162990174089
4	0.45848876572952013	1.5956608417685623
5	0.2170904421926583	0.9444144099634347
6	0.1284708631926522	0.6706683163061057
7	0.07334222832193937	0.4466877455361989
8	0.053485474580830354	0.37228688583565794
9	0.033083415914718105	0.25906261892129534
>10	0.12833312469081037	1.7656889337424126
>50	0.0025614421562854327	0.14850503718616367
>100	4.800967013661216E-4	0.06803151379874547
>500	5.392544782394701E-5	0.034602510670434926
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4647514163047632E-5
2	0.0	1.4647514163047632E-5	0.0	1.4647514163047632E-5	1.4647514163047632E-5
3	0.0	2.9295028326095264E-5	0.0	1.4647514163047632E-5	1.4647514163047632E-5
4	0.0	4.3942542489142895E-5	0.0	1.4647514163047632E-5	1.4647514163047632E-5
5	0.0	7.323757081523817E-5	0.0	1.4647514163047632E-5	1.4647514163047632E-5
6	4.3942542489142895E-5	7.323757081523817E-5	0.0	1.4647514163047632E-5	1.4647514163047632E-5
7	4.3942542489142895E-5	7.323757081523817E-5	0.0	1.4647514163047632E-5	1.4647514163047632E-5
8	4.3942542489142895E-5	8.788508497828579E-5	0.0	1.4647514163047632E-5	1.4647514163047632E-5
9	4.3942542489142895E-5	2.0506519828266686E-4	0.0	1.4647514163047632E-5	1.4647514163047632E-5
10-11	4.3942542489142895E-5	2.0506519828266686E-4	0.0	1.4647514163047632E-5	1.4647514163047632E-5
12-13	4.3942542489142895E-5	2.0506519828266686E-4	0.0	1.4647514163047632E-5	1.4647514163047632E-5
14-15	8.056132789676198E-5	2.709790120163812E-4	0.0	1.4647514163047632E-5	1.4647514163047632E-5
16-17	1.3182762746742868E-4	2.78302769097905E-4	0.0	4.3942542489142895E-5	1.4647514163047632E-5
18-19	1.3182762746742868E-4	3.222453115870479E-4	0.0	5.859005665219053E-5	3.6618785407619076E-5
20-21	1.391513845489525E-4	3.661878540761908E-4	0.0	1.0985635622285724E-4	4.3942542489142895E-5
22-23	1.4647514163047633E-4	4.3942542489142897E-4	0.0	3.2956906866857174E-4	4.3942542489142895E-5
24-25	1.4647514163047633E-4	4.613966961360004E-4	0.0	0.001193772404288382	4.3942542489142895E-5
26-27	1.6112265579352396E-4	4.8336796738057187E-4	0.0	0.0036545547836803844	4.3942542489142895E-5
28-29	1.6112265579352396E-4	4.8336796738057187E-4	0.0	0.012289264382796962	4.3942542489142895E-5
30-31	1.6112265579352396E-4	5.859005665219053E-4	0.0	0.013534303086656011	4.3942542489142895E-5
32-33	1.6112265579352396E-4	6.151955948480005E-4	0.0	0.016515072218836205	4.3942542489142895E-5
34-35	1.6112265579352396E-4	6.81109408581715E-4	0.0	0.020294130872902494	4.3942542489142895E-5
36-37	1.6112265579352396E-4	7.689944935600007E-4	0.0	0.0275226791123665	7.323757081523817E-5
38-39	1.6112265579352396E-4	7.909657648045721E-4	0.0	0.04620558342733376	1.0985635622285724E-4
40-41	1.6112265579352396E-4	8.934983639459056E-4	0.0	0.06541579825217073	1.1718011330438105E-4
42-43	1.7577016995657158E-4	9.374409064350484E-4	0.0	0.07771238639204921	1.1718011330438105E-4
44-45	1.7577016995657158E-4	0.0010472972626579058	0.0	0.09316551383406446	1.2450387038590488E-4
46-47	1.7577016995657158E-4	0.00111321107639162	0.0	0.1099955076074062	1.7577016995657158E-4
48-49	1.7577016995657158E-4	0.0011205348334731439	0.0	0.126393399712938	1.7577016995657158E-4
50-51	1.7577016995657158E-4	0.0012450387038590487	0.0	0.141758642069975	1.7577016995657158E-4
52-53	1.7577016995657158E-4	0.0012523624609405725	0.0	0.18119707395398074	1.7577016995657158E-4
54-55	1.8309392703809538E-4	0.001318276274674287	0.0	0.22806911927573315	1.7577016995657158E-4
56-57	1.904176841196192E-4	0.0013475713030003823	0.0	0.27471412812795837	1.7577016995657158E-4
58-59	1.904176841196192E-4	0.00136221881716343	0.0	0.46579827414199626	1.7577016995657158E-4
60-61	1.9774144120114303E-4	0.0014134851167340965	0.0	0.6368885633234741	1.7577016995657158E-4
62-63	2.1971271244571448E-4	0.0014427801450601918	0.0	0.644871458542335	1.7577016995657158E-4
64-65	2.1971271244571448E-4	0.00166981661458743	0.0	0.6511918609036901	1.8309392703809538E-4
66-67	2.2703646952723828E-4	0.0017357304283211444	0.0	0.6572632555242733	1.904176841196192E-4
68-69	2.343602266087621E-4	0.0017723492137287635	0.0	0.6635616866143839	1.904176841196192E-4
70-71	2.4900774077180976E-4	0.001794320484973335	0.0	0.6689959143688745	2.0506519828266686E-4
72-73	2.9295028326095266E-4	0.0018016442420548588	0.0	0.6731851034195061	2.0506519828266686E-4
74-75	2.9295028326095266E-4	0.0018822055699516207	0.0	0.6765100891345179	2.0506519828266686E-4
76-77	2.9295028326095266E-4	0.001962766897848383	0.0	0.6795494483233503	2.0506519828266686E-4
78-79	2.9295028326095266E-4	0.0019700906549299066	0.0	0.6816879853911553	2.0506519828266686E-4
80-81	2.9295028326095266E-4	0.001999385683256002	0.0	0.6824350086134707	2.0506519828266686E-4
82-83	2.9295028326095266E-4	0.0020067094403375257	0.0	0.6828817577954436	2.0506519828266686E-4
84-85	2.9295028326095266E-4	0.0020286807115820972	0.0	0.683123441779134	2.0506519828266686E-4
86-87	2.9295028326095266E-4	0.0020726232540712397	0.0	0.6832113268641122	2.0506519828266686E-4
88	2.9295028326095266E-4	0.0021238895536419065	0.0	0.6832186506211938	2.0506519828266686E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8140	0.0	51.394554	1
GTATCAA	14360	0.0	36.017525	1
TCAACGC	17380	0.0	29.099869	4
ATCAACG	17640	0.0	28.67096	3
CAACGCA	18010	0.0	27.987707	5
TATCAAC	18400	0.0	27.602022	2
AACGCAG	18580	0.0	27.10626	6
CGAGACC	4795	0.0	25.174776	86-87
GAGACCG	4740	0.0	24.949347	88-89
GACCGAG	4770	0.0	24.87752	90-91
AGACCGA	4935	0.0	24.664879	88-89
ACCGAGG	5085	0.0	24.15321	90-91
ACGCAGA	21285	0.0	23.761135	7
CACGAGA	5140	0.0	23.678345	84-85
ACGAGAC	5020	0.0	23.426964	86-87
TCTCCGA	5010	0.0	22.728607	74-75
CCCACGA	5220	0.0	22.686733	82-83
GCCCACG	5155	0.0	21.964748	82-83
CGCAGAG	23195	0.0	21.767931	8
GTGGTAT	3665	0.0	21.417183	1
>>END_MODULE