FastQCFastQC Report
Fri 27 May 2016
SRR2139201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6767422
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82750.051.918951
GTATCAA151500.036.723671
TCAACGC186050.029.2682114
ATCAACG188200.028.9338513
CAACGCA190250.028.6220825
TATCAAC194700.028.1414812
AACGCAG195350.027.9180976
CGAGACC51650.024.58911586-87
ACGCAGA225300.024.2443357
GAGACCG52700.024.00112288-89
GACCGAG54000.023.93588890-91
ACCGAGG56100.023.73322390-91
AGACCGA54550.023.61358388-89
GTGGTAT38700.022.9235171
CGCAGAG244350.022.3714898
TGGTATC37500.022.1925872
ACGAGAC56550.022.0556586-87
CACGAGA58250.021.93978984-85
CCCACGA57750.021.59209482-83
ATCTCCG55550.021.48374674-75
Produced by FastQC (version 0.11.5)