##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6767422
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.997198490060175	31.0	31.0	34.0	30.0	34.0
2	32.17533899910483	33.0	31.0	34.0	30.0	34.0
3	32.19026033842725	34.0	31.0	34.0	30.0	34.0
4	35.953687829723044	37.0	35.0	37.0	35.0	37.0
5	35.81238527758428	37.0	35.0	37.0	35.0	37.0
6	35.83565706409324	37.0	35.0	37.0	35.0	37.0
7	35.77487749397038	37.0	35.0	37.0	33.0	37.0
8	35.762772293496695	37.0	35.0	37.0	33.0	37.0
9	37.53366732560789	39.0	37.0	39.0	35.0	39.0
10-11	37.408046668288165	39.0	37.0	39.0	34.0	39.0
12-13	37.383469879667615	39.0	37.0	39.0	34.0	39.0
14-15	38.611186135577185	40.0	38.0	41.0	34.0	41.0
16-17	38.56658229677416	40.0	38.0	41.0	34.0	41.0
18-19	38.586627670034474	40.0	38.0	41.0	34.0	41.0
20-21	38.56669941781262	40.0	38.0	41.0	34.0	41.0
22-23	38.482573382407544	40.0	38.0	41.0	34.0	41.0
24-25	38.42112442607565	40.0	38.0	41.0	34.0	41.0
26-27	38.2416106129579	40.0	38.0	41.0	33.5	41.0
28-29	38.1281905297152	40.0	38.0	41.0	33.0	41.0
30-31	37.97571759376342	40.0	37.0	41.0	33.0	41.0
32-33	37.79853575842006	40.0	37.0	41.0	33.0	41.0
34-35	37.886147340184515	40.0	37.0	41.0	33.0	41.0
36-37	37.85690314093189	40.0	37.0	41.0	33.0	41.0
38-39	37.81152373682377	40.0	37.0	41.0	33.0	41.0
40-41	37.727077250052105	40.0	37.0	41.0	33.0	41.0
42-43	37.623372569944046	40.0	37.0	41.0	32.0	41.0
44-45	37.67627193002332	40.0	37.0	41.0	32.0	41.0
46-47	37.668152220529194	40.0	37.0	41.0	32.0	41.0
48-49	37.60119595982428	40.0	36.5	41.0	32.0	41.0
50-51	37.49459491076318	40.0	36.0	41.0	32.0	41.0
52-53	37.29318248584177	39.0	36.0	41.0	31.0	41.0
54-55	37.15674505190971	39.0	35.5	41.0	31.0	41.0
56-57	36.97522656819415	39.0	35.0	41.0	31.0	41.0
58-59	36.823168325325824	39.0	35.0	40.0	31.0	41.0
60-61	36.5944062291818	38.0	35.0	40.0	31.0	41.0
62-63	36.37039422194507	38.0	35.0	40.0	30.0	41.0
64-65	36.05992453363386	37.5	34.5	40.0	30.0	41.0
66-67	35.76671899969274	37.0	34.0	40.0	29.5	41.0
68-69	35.463346986191034	36.5	34.0	39.0	29.0	41.0
70-71	35.175120470945345	36.0	34.0	39.0	29.0	41.0
72-73	34.90026258796462	35.5	34.0	39.0	29.0	40.0
74-75	34.548643857964905	35.0	33.0	37.5	29.0	40.0
76-77	33.28364810287786	34.5	31.5	36.0	27.0	39.0
78-79	33.97738105347406	35.0	33.0	37.0	29.0	39.0
80-81	33.91517042861453	35.0	33.0	36.0	29.0	39.0
82-83	33.65567046898036	35.0	33.0	36.0	29.0	37.5
84-85	33.50056091809893	35.0	33.0	35.5	29.0	37.0
86-87	33.28798627612716	35.0	33.0	35.0	29.0	37.0
88-89	33.201081596825915	35.0	33.0	35.0	29.0	36.0
90-91	33.086749981582045	35.0	33.0	35.0	29.0	36.0
92-93	32.9681930660693	35.0	33.0	35.0	29.0	36.0
94-95	32.94127231037935	35.0	33.0	35.0	29.0	35.5
96-97	33.01905968780496	34.0	33.0	35.0	29.0	35.0
98-99	33.16871503210511	34.0	33.0	35.0	29.0	35.0
100	31.98115540284574	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	4.0
20	40.0
21	230.0
22	1271.0
23	4170.0
24	11213.0
25	24189.0
26	43927.0
27	69665.0
28	99317.0
29	130698.0
30	164554.0
31	202496.0
32	247082.0
33	309061.0
34	404518.0
35	554968.0
36	846336.0
37	1366642.0
38	1545659.0
39	715553.0
40	25825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60975922589134	20.14306186314375	14.316204900477612	23.9309740104873
2	15.023165394444145	21.41019135499456	40.55018292046809	23.016460330093203
3	18.17510419772847	26.216364222594663	30.551929523532007	25.056602056144868
4	12.83064658890786	18.35334045963145	38.37978479840625	30.43622815305444
5	13.46437387826561	37.62679495973504	35.42563179893318	13.483199363066174
6	30.052241459155347	37.811680725688454	18.6947555509321	13.441322264224102
7	26.72969115861254	32.499894346768976	23.067971821470568	17.702442673147914
8	24.917479654734105	36.164436028963465	20.848633349597527	18.0694509667049
9	25.811941386247227	16.603383090340753	20.980057102985448	36.60461842042657
10-11	24.419956373342757	26.527553623817163	28.62708280937704	20.425407193463034
12-13	25.365645199256853	24.17664720430427	28.570026897986693	21.887680698452186
14-15	22.427432484630042	25.363203299572568	26.325475195724458	25.88388902007293
16-17	22.036187835221344	28.597634201949152	26.82268720269966	22.543490760129842
18-19	22.140812070023976	27.736188549350587	28.450696920142793	21.67230246048264
20-21	23.043255797973362	26.846930252163858	28.378303792857455	21.731510157005317
22-23	22.822285490317558	26.756884297222634	28.182160387380456	22.23866982507935
24-25	22.57119881268792	26.905567890540233	28.328538949808273	22.194694346963573
26-27	22.437510107101446	27.01964750275198	28.28440035856502	22.258442031581556
28-29	22.4518748615074	27.05248901954822	28.258379935284417	22.237256183659962
30-31	22.820633585363765	26.78134955051429	28.17903099301689	22.21898587110506
32-33	22.593185717702465	26.94113351678547	28.07471861601083	22.390962149501238
34-35	22.714137279102104	27.011434276430407	28.091792130257925	22.182636314209564
36-37	22.78811202547581	26.911789280584465	27.92201458312545	22.37808411081428
38-39	22.49171513581218	27.15336867145256	28.037739642546544	22.317176550188723
40-41	22.668546241091022	26.95551362451587	28.001631624117433	22.37430851027567
42-43	22.573426206358384	27.1035229762622	27.73061197272831	22.592438844651106
44-45	22.561331926354416	27.166613825400237	27.615554148850386	22.65650009939496
46-47	22.727858650131836	27.123521910638328	27.41230978834247	22.73630965088737
48-49	22.741516050841888	27.331130357393018	27.27682900174268	22.65052459002241
50-51	22.72539388040033	27.370951990946125	27.236537248867972	22.667116879785574
52-53	22.88687370607682	27.250818987701603	27.178887051142443	22.683420255079138
54-55	22.897715764998058	27.20354823191831	27.140262756533918	22.758473246549713
56-57	22.904200199936533	27.38283045221388	26.960988209064485	22.7519811387851
58-59	22.71389261808557	27.279172655253745	27.14118057962879	22.86575414703189
60-61	22.956026908341855	27.26916932986221	27.152555433363272	22.622248328432658
62-63	22.737322021355112	27.273581070173726	27.251625415945053	22.737471492526108
64-65	22.80613951754612	27.20976640254012	27.15204249445053	22.83205158546323
66-67	22.84758920459754	27.322748553163862	27.109854924050786	22.719807318187815
68-69	22.775926584096442	27.406531113953836	27.100979371985574	22.716562929964145
70-71	22.77030762863748	27.269141030946876	26.91170781220086	23.048843528214785
72-73	22.784251349166468	27.30415783608993	26.78572833140335	23.12586248334025
74-75	22.706736394215625	27.346437250947275	27.018394724750422	22.928431630086674
76-77	22.8281843714746	27.14097382589972	26.947065144423394	23.083776658202286
78-79	22.71209554125001	27.124251320869142	26.799482443057315	23.364170694823535
80-81	22.92800296233261	27.32546216994519	26.849118428238373	22.897416439483827
82-83	23.087336037680757	26.99854259828302	26.985645074200548	22.92847628983568
84-85	22.856268515610413	27.051593095367473	26.930861971887982	23.161276417134133
86-87	22.685861429842106	27.35929566511821	26.924771934127495	23.030070970912185
88-89	23.100360517560187	27.166720549500933	26.83722332737124	22.89569560556764
90-91	23.10947965868775	27.145690519010724	26.921877283895878	22.82295253840565
92-93	22.885038025581984	26.960505145938196	27.06090009248685	23.093556735992962
94-95	22.811116055185632	27.30971217861779	27.1086486299102	22.77052313628638
96-97	23.07128195909904	27.171233048509276	27.227459324783037	22.53002566760865
98-99	23.91211651294609	29.358448608401588	27.376764621279452	19.35267025737287
100	22.866945195234983	30.391731097253384	21.15050553229577	25.590818175215862
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1507.0
1	1298.0
2	1608.0
3	2240.0
4	2844.5
5	4529.5
6	6154.5
7	7667.5
8	9098.5
9	9488.5
10	8853.0
11	7941.5
12	8229.5
13	10352.0
14	15790.5
15	24091.5
16	31179.5
17	35210.5
18	36374.0
19	35073.0
20	32309.0
21	29429.5
22	27520.0
23	28228.5
24	32666.5
25	40492.5
26	50869.0
27	62489.0
28	75193.0
29	89494.0
30	103843.5
31	117613.0
32	132306.0
33	146242.0
34	159598.5
35	172804.5
36	186326.0
37	199818.5
38	211226.0
39	221061.5
40	230808.0
41	239395.0
42	245294.5
43	250980.0
44	257563.5
45	266803.5
46	275863.5
47	285512.0
48	290962.5
49	288554.0
50	286031.0
51	283101.0
52	271381.0
53	254335.0
54	241772.0
55	229359.5
56	214637.0
57	196887.5
58	177190.0
59	156202.0
60	133410.5
61	114002.5
62	94985.5
63	77167.5
64	63271.5
65	49900.0
66	39283.0
67	29647.5
68	20801.5
69	15432.0
70	11440.0
71	7820.0
72	5469.5
73	3958.0
74	2901.5
75	2028.5
76	1276.0
77	718.5
78	496.5
79	367.5
80	256.5
81	174.0
82	122.5
83	84.0
84	60.5
85	45.5
86	33.0
87	22.5
88	12.0
89	6.0
90	5.5
91	5.5
92	4.5
93	4.0
94	3.5
95	3.0
96	3.0
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.4776675667632372E-5
14-15	0.0
16-17	9.604839183961041E-4
18-19	0.0016475993369410094
20-21	0.0
22-23	0.002822746727645878
24-25	2.512979910795126E-4
26-27	0.0016710867898149183
28-29	0.005008603404577819
30-31	0.0017259692669319622
32-33	0.005308885039101608
34-35	0.010799730468519866
36-37	5.535327507376198E-4
38-39	3.762254698171988E-5
40-41	0.0025299381058974525
42-43	0.005721774809100613
44-45	0.0016167780293108
46-47	0.0016792874969737838
48-49	0.0030606851461418296
50-51	0.010180219169194371
52-53	0.00613490080447776
54-55	0.00998509872746268
56-57	0.017781408835698
58-59	0.012454818614147026
60-61	0.01575615170763362
62-63	0.014787155051831013
64-65	0.010767600900590408
66-67	0.013253836270434798
68-69	0.00887809832160321
70-71	0.008897207167534758
72-73	0.005942533948909938
74-75	0.010907356972534568
76-77	0.01149068874759796
78-79	0.006573324851818025
80-81	0.003563883712119343
82-83	0.0019244141916664723
84-85	2.8100959629037362E-5
86-87	0.003881983156179491
88-89	5.969116944243441E-4
90-91	5.478874146151707E-4
92-93	0.001454112009650523
94-95	0.001047089122736534
96-97	4.493901514371654E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	191.0
20-21	561.0
22-23	1100.0
24-25	2760.0
26-27	3033.0
28-29	6524.0
30-31	14829.0
32-33	19538.0
34-35	25807.0
36-37	37654.0
38-39	43214.0
40-41	45750.0
42-43	56375.0
44-45	63124.0
46-47	61678.0
48-49	56028.0
50-51	50160.0
52-53	47091.0
54-55	51399.0
56-57	55808.0
58-59	33869.0
60-61	55364.0
62-63	54334.0
64-65	58239.0
66-67	61275.0
68-69	63243.0
70-71	61995.0
72-73	60163.0
74-75	61324.0
76-77	61165.0
78-79	65371.0
80-81	67784.0
82-83	65856.0
84-85	68971.0
86-87	72901.0
88-89	80752.0
90-91	88144.0
92-93	98774.0
94-95	116464.0
96-97	287850.0
98-99	465256.0
100-101	4075704.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.52408865364906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.96695518038919	78.70832894580515
2	6.378584521615753	11.038024252661502
3	1.4756529274214925	3.830385741427019
4	0.5256924596035116	1.8194024391715624
5	0.23908910795959623	1.0343483586608986
6	0.1319012858406898	0.684758312976609
7	0.07661245182142425	0.464017580135923
8	0.05071182019063339	0.3510235220769819
9	0.0320349680018792	0.24946167702702662
>10	0.11965161735811573	1.5872716481859495
>50	0.0026886763726583066	0.15579419527990546
>100	3.9773093077599496E-4	0.061620544872694805
>500	2.7252494268375028E-5	0.015562781718725629
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4776675667632372E-5
2	1.4776675667632372E-5	5.910670267052949E-5	0.0	0.0	1.4776675667632372E-5
3	1.4776675667632372E-5	5.910670267052949E-5	0.0	0.0	1.4776675667632372E-5
4	1.4776675667632372E-5	1.1821340534105898E-4	0.0	5.910670267052949E-5	1.4776675667632372E-5
5	1.4776675667632372E-5	1.1821340534105898E-4	0.0	5.910670267052949E-5	1.4776675667632372E-5
6	5.910670267052949E-5	1.1821340534105898E-4	0.0	5.910670267052949E-5	1.4776675667632372E-5
7	7.388337833816186E-5	1.1821340534105898E-4	0.0	5.910670267052949E-5	1.4776675667632372E-5
8	7.388337833816186E-5	1.1821340534105898E-4	0.0	7.388337833816186E-5	2.9553351335264744E-5
9	7.388337833816186E-5	2.068734593468532E-4	0.0	8.866005400579423E-5	2.9553351335264744E-5
10-11	8.127171617197805E-5	2.068734593468532E-4	0.0	1.108250675072428E-4	2.9553351335264744E-5
12-13	8.866005400579423E-5	2.068734593468532E-4	0.0	1.2560174317487516E-4	3.694168916908093E-5
14-15	8.866005400579423E-5	2.4381514851593413E-4	0.0	1.7732010801158846E-4	6.649504050434568E-5
16-17	1.3299008100869136E-4	2.659801620173827E-4	0.0	2.068734593468532E-4	7.388337833816186E-5
18-19	1.3299008100869136E-4	2.9553351335264745E-4	0.0	2.142617971806694E-4	8.866005400579423E-5
20-21	1.3299008100869136E-4	3.250868646879122E-4	0.0	2.585918241835665E-4	1.034367296734266E-4
22-23	1.3299008100869136E-4	3.9158190519225785E-4	0.0	5.393486618685816E-4	1.1821340534105898E-4
24-25	1.3299008100869136E-4	4.654652835304197E-4	0.0	0.0015072209180985018	1.4776675667632373E-4
26-27	1.4776675667632373E-4	5.17183648367133E-4	0.0	0.004440391038123527	1.4776675667632373E-4
28-29	1.4776675667632373E-4	5.245719862009492E-4	0.0	0.01489488907297343	1.625434323439561E-4
30-31	1.5515509451013992E-4	5.467369997023977E-4	0.0	0.01649815838291154	1.7732010801158846E-4
32-33	1.625434323439561E-4	5.54125337536214E-4	0.0	0.01975641536762448	1.7732010801158846E-4
34-35	1.625434323439561E-4	6.945037563787214E-4	0.0	0.02396037959506589	1.7732010801158846E-4
36-37	1.625434323439561E-4	8.274938373874128E-4	0.0	0.03115662064520286	1.7732010801158846E-4
38-39	1.625434323439561E-4	8.570471887226776E-4	0.0	0.0499377754187636	2.068734593468532E-4
40-41	1.625434323439561E-4	9.53095580562288E-4	0.0	0.07113491666398224	2.068734593468532E-4
42-43	1.625434323439561E-4	9.974256075651851E-4	0.0	0.0840792845488282	2.068734593468532E-4
44-45	1.625434323439561E-4	0.0011378040264076925	0.0	0.10096163649909817	2.068734593468532E-4
46-47	1.9209678367922083E-4	0.0012412407560811192	0.0	0.11910739421895074	2.216501350144856E-4
48-49	1.99485121513037E-4	0.0012412407560811192	0.0	0.13717188022263133	2.3642681068211796E-4
50-51	2.216501350144856E-4	0.001389007512757443	0.0	0.1533301750651873	2.3642681068211796E-4
52-53	2.216501350144856E-4	0.0014037841884250754	0.0	0.19602737940681103	2.733684998511989E-4
54-55	2.216501350144856E-4	0.0014185608640927077	0.0	0.24650450348744324	2.8075683768501506E-4
56-57	2.2903847284830179E-4	0.0014481142154279725	0.0	0.2973141027705971	2.8814517551883125E-4
58-59	2.3642681068211796E-4	0.0014481142154279725	0.0	0.4956761969328941	2.9553351335264745E-4
60-61	2.4381514851593413E-4	0.0014628908910956048	0.0	0.6728780915391415	3.17698526854096E-4
62-63	2.8075683768501506E-4	0.0015072209180985018	0.0	0.6817367086018871	3.472518781893607E-4
64-65	2.8075683768501506E-4	0.0016845410261100903	0.0	0.6893171432193825	3.546402160231769E-4
66-67	2.8075683768501506E-4	0.0017140943774453551	0.0	0.6959370939184818	3.546402160231769E-4
68-69	2.8814517551883125E-4	0.001751036066614436	0.0	0.702158074374555	3.620285538569931E-4
70-71	2.9553351335264745E-4	0.001787977755783517	0.0	0.7071895324393839	3.694168916908093E-4
72-73	3.103101890202798E-4	0.0017953660936173331	0.0	0.7114599917073297	4.137469186937064E-4
74-75	3.103101890202798E-4	0.0018470844584540466	0.0	0.7148881804622202	4.137469186937064E-4
76-77	3.103101890202798E-4	0.0019209678367922082	0.0	0.7180208357037584	4.211352565275226E-4
78-79	3.103101890202798E-4	0.0019652978637951053	0.0	0.7200230752567225	4.285235943613388E-4
80-81	3.103101890202798E-4	0.0019726862016289214	0.0	0.7209983358507863	4.433002700289712E-4
82-83	3.103101890202798E-4	0.001980074539462738	0.0	0.7215081311613196	5.097953105333169E-4
84-85	3.103101890202798E-4	0.0019948512151303703	0.0	0.7217371696341679	5.245719862009492E-4
86-87	3.103101890202798E-4	0.002039181242133267	0.0	0.7218627713773429	5.319603240347654E-4
88	3.103101890202798E-4	0.002142617971806694	0.0	0.7218701597151767	5.319603240347654E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8275	0.0	51.91895	1
GTATCAA	15150	0.0	36.72367	1
TCAACGC	18605	0.0	29.268211	4
ATCAACG	18820	0.0	28.933851	3
CAACGCA	19025	0.0	28.622082	5
TATCAAC	19470	0.0	28.141481	2
AACGCAG	19535	0.0	27.918097	6
CGAGACC	5165	0.0	24.589115	86-87
ACGCAGA	22530	0.0	24.244335	7
GAGACCG	5270	0.0	24.001122	88-89
GACCGAG	5400	0.0	23.935888	90-91
ACCGAGG	5610	0.0	23.733223	90-91
AGACCGA	5455	0.0	23.613583	88-89
GTGGTAT	3870	0.0	22.923517	1
CGCAGAG	24435	0.0	22.371489	8
TGGTATC	3750	0.0	22.192587	2
ACGAGAC	5655	0.0	22.05565	86-87
CACGAGA	5825	0.0	21.939789	84-85
CCCACGA	5775	0.0	21.592094	82-83
ATCTCCG	5555	0.0	21.483746	74-75
>>END_MODULE