FastQCFastQC Report
Fri 27 May 2016
SRR2139200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9044251
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107100.051.6478081
GTATCAA198700.037.7350121
TCAACGC239900.030.7080044
ATCAACG243250.030.2850953
CAACGCA246400.029.915095
TATCAAC252200.029.3947982
AACGCAG254950.028.9947956
ACGCAGA290500.025.461117
GACCGAG71100.025.41428890-91
GAGACCG69050.025.40968188-89
AGACCGA71500.025.38935988-89
CGAGACC70600.025.2936886-87
ACCGAGG76200.024.22119590-91
ACGAGAC74850.023.51613686-87
CGCAGAG318250.023.2277248
CACGAGA76300.023.2258584-85
CCCACGA76550.022.65488882-83
GCCCACG76350.022.2102782-83
TCTCCGA76450.022.08760674-75
ATCTCCG78050.021.0663674-75
Produced by FastQC (version 0.11.5)