##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9044251
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00203532608726	31.0	31.0	34.0	30.0	34.0
2	32.1824894068066	33.0	31.0	34.0	30.0	34.0
3	32.197813395492894	34.0	31.0	34.0	30.0	34.0
4	35.960618297745164	37.0	35.0	37.0	35.0	37.0
5	35.821031172177776	37.0	35.0	37.0	35.0	37.0
6	35.84113819928262	37.0	35.0	37.0	35.0	37.0
7	35.77528410036387	37.0	35.0	37.0	33.0	37.0
8	35.76653268468555	37.0	35.0	37.0	33.0	37.0
9	37.5376211916277	39.0	37.0	39.0	35.0	39.0
10-11	37.41177500491749	39.0	37.0	39.0	34.0	39.0
12-13	37.3887344015552	39.0	37.0	39.0	34.0	39.0
14-15	38.623034068824495	40.0	38.0	41.0	34.0	41.0
16-17	38.57884185213347	40.0	38.0	41.0	34.0	41.0
18-19	38.59311235391411	40.0	38.0	41.0	34.0	41.0
20-21	38.57135120687188	40.0	38.0	41.0	34.0	41.0
22-23	38.488801379395994	40.0	38.0	41.0	34.0	41.0
24-25	38.429819531237605	40.0	38.0	41.0	34.0	41.0
26-27	38.25643927421921	40.0	38.0	41.0	33.5	41.0
28-29	38.146511529031656	40.0	38.0	41.0	33.0	41.0
30-31	37.99437825058743	40.0	37.0	41.0	33.0	41.0
32-33	37.818656139478186	40.0	37.0	41.0	33.0	41.0
34-35	37.90703460603974	40.0	37.0	41.0	33.0	41.0
36-37	37.87709152837107	40.0	37.0	41.0	33.0	41.0
38-39	37.83060633758777	40.0	37.0	41.0	33.0	41.0
40-41	37.74422393563988	40.0	37.0	41.0	33.0	41.0
42-43	37.639328857227106	40.0	37.0	41.0	32.0	41.0
44-45	37.69035502859103	40.0	37.0	41.0	32.0	41.0
46-47	37.68035011250241	40.0	37.0	41.0	32.0	41.0
48-49	37.610098847760796	40.0	36.5	41.0	32.0	41.0
50-51	37.49897527755659	39.5	36.0	41.0	32.0	41.0
52-53	37.29362631537881	39.0	36.0	41.0	31.0	41.0
54-55	37.152237222243386	39.0	35.5	41.0	31.0	41.0
56-57	36.97045412602863	39.0	35.0	41.0	31.0	41.0
58-59	36.81283099731485	39.0	35.0	40.0	31.0	41.0
60-61	36.58043267583973	38.0	35.0	40.0	31.0	41.0
62-63	36.35140999390214	38.0	35.0	40.0	30.0	41.0
64-65	36.038527329743516	37.0	34.0	40.0	30.0	41.0
66-67	35.74196801677473	37.0	34.0	40.0	29.5	41.0
68-69	35.43781663516362	36.0	34.0	39.0	29.0	41.0
70-71	35.15014578763666	36.0	34.0	39.0	29.0	40.5
72-73	34.87118873785706	35.5	34.0	38.5	29.0	40.0
74-75	34.51634057337675	35.0	33.0	37.5	29.0	40.0
76-77	33.252289584657184	34.5	31.5	36.0	27.0	39.0
78-79	33.94329660551435	35.0	33.0	36.5	29.0	39.0
80-81	33.88366583571327	35.0	33.0	36.0	29.0	39.0
82-83	33.625633226863755	35.0	33.0	36.0	29.0	37.0
84-85	33.47610643342642	35.0	33.0	35.5	29.0	37.0
86-87	33.269261213199435	35.0	33.0	35.0	29.0	36.5
88-89	33.19123027746081	35.0	33.0	35.0	29.0	36.0
90-91	33.07891306357239	35.0	33.0	35.0	29.0	36.0
92-93	32.964123454092885	35.0	33.0	35.0	29.0	36.0
94-95	32.939283661374766	35.0	33.0	35.0	29.0	35.0
96-97	33.01644807631459	34.0	33.0	35.0	29.0	35.0
98-99	33.168547923530355	34.0	33.0	35.0	29.0	35.0
100	31.98589996590754	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	45.0
21	319.0
22	1474.0
23	5355.0
24	14777.0
25	31889.0
26	58280.0
27	91585.0
28	131615.0
29	173710.0
30	219041.0
31	268496.0
32	330429.0
33	413119.0
34	540311.0
35	746566.0
36	1144810.0
37	1838337.0
38	2061293.0
39	938140.0
40	34655.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.52518544653393	20.15824195945026	14.351227094427166	23.965345499588633
2	15.107707647653742	21.43242154601857	40.38057988439286	23.079290921934827
3	18.10691675850217	26.228849685839105	30.54853298520795	25.115700570450777
4	12.926366152376797	18.373141125782556	38.23902056676667	30.46147215507398
5	13.588941748741826	37.59568371112213	35.318314363455855	13.497060176680192
6	30.19030542164299	37.626476753022445	18.716077207498998	13.467140617835573
7	26.802595372463678	32.409637901469125	23.11574501857589	17.67202170749131
8	24.99167703328888	35.9886739100894	20.896423595497293	18.12322546112442
9	25.95547160290001	16.591644791813053	20.927028672689424	36.52585493259751
10-11	24.570647143693822	26.450946573685318	28.56475345498483	20.41365282763603
12-13	25.479759265818576	24.108219261310737	28.429993002736158	21.982028470134534
14-15	22.57609406108334	25.247542623902337	26.18020144400025	25.996161871014078
16-17	22.24298637613387	28.45506862960436	26.67983574603981	22.62210924822196
18-19	22.349683478696996	27.60500520836846	28.22796389115157	21.817347421782976
20-21	23.320229253487625	26.738702893466414	28.078250303202427	21.862817549843538
22-23	23.05581965901026	26.63610408345565	27.86880169773288	22.43927455980121
24-25	22.803725850955786	26.81899700846183	28.035228829552672	22.342048311029714
26-27	22.68555614142153	26.88522295397067	27.985516139009214	22.44370476559859
28-29	22.732313837841463	26.943089173430902	27.943903129584456	22.38069385914318
30-31	23.067310701504795	26.66116101207227	27.827885676758175	22.443642609664757
32-33	22.832654006831877	26.82211034840241	27.745688825405452	22.599546819360263
34-35	22.960002349534626	26.912308579942053	27.748745136181892	22.37894393434143
36-37	23.063194731931304	26.789253861501116	27.60071385155617	22.546837555011415
38-39	22.70520027688404	27.07214603196008	27.698140566882866	22.524513124273014
40-41	22.944829081397522	26.832938297809783	27.696619516010625	22.525613104782067
42-43	22.78931417219238	26.9781550212984	27.430356578426373	22.802174228082848
44-45	22.798043938953285	27.02882096351423	27.315784831903233	22.85735026562925
46-47	22.98171480258028	26.976869408061976	27.152640215158673	22.888775574199073
48-49	23.021658740877072	27.19066161190064	26.94641753254349	22.841262114678802
50-51	22.942902196872218	27.226647112774444	26.994518602852864	22.835932087500474
52-53	23.167957696220252	27.08773615132764	26.889714424922563	22.854591727529545
54-55	23.183501417884827	26.98165912291936	26.883262157705996	22.951577301489813
56-57	23.144183337537548	27.205156160709077	26.738170402053846	22.912490099699532
58-59	22.947135033566777	27.088344379678354	26.90911369685967	23.055406889895195
60-61	23.152050992715843	27.128237688489875	26.925269280907628	22.794442037886657
62-63	22.977151880209792	27.107871980865244	27.008920915497686	22.906055223427277
64-65	23.024430020544532	27.07148807225886	26.891639692017943	23.012442215178666
66-67	23.076830653417307	27.177050636440665	26.858512537362873	22.887606172779158
68-69	23.012832659071	27.29972236006407	26.805920130828515	22.88152485003641
70-71	22.9974721613434	27.119358329011302	26.645072019627314	23.238097490017985
72-73	23.00265121202508	27.17772744245038	26.498200522564357	23.32142082296019
74-75	22.91190933006649	27.253120912886033	26.74936313522424	23.08560662182324
76-77	23.07141205669397	27.00670845100995	26.675648534961976	23.246230957334102
78-79	22.869831383706668	27.082719377939778	26.519783691640452	23.5276655467131
80-81	23.1626418066388	27.249905834262023	26.533256444397296	23.054195914701882
82-83	23.29441276214202	26.93633613607976	26.701976549529487	23.067274552248733
84-85	23.036690126828987	27.003629731689475	26.61654230017325	23.343137841308284
86-87	22.859262432370826	27.337522722607847	26.64044329355625	23.162771551465077
88-89	23.267274676654036	27.191636564808018	26.514696034417785	23.02639272412016
90-91	23.269386580052792	27.217155703533418	26.568216055920914	22.945241660492876
92-93	23.034735719050463	27.0455921031566	26.649127403974916	23.270544773818024
94-95	22.991282195845763	27.402614954667587	26.732530073358234	22.873572776128416
96-97	23.217825618085858	27.278595011597073	26.84795116771334	22.655628202603726
98-99	24.04980492377702	29.417404974041588	27.056269218519347	19.476520883662047
100	22.929871697361477	30.510171161732657	20.798140770738634	25.761816370167228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3244.0
1	2597.5
2	2634.5
3	3448.5
4	4236.0
5	6360.0
6	8359.5
7	10029.0
8	11351.5
9	11521.5
10	10689.5
11	9563.0
12	9995.5
13	12924.0
14	19868.5
15	30178.0
16	39269.5
17	45022.0
18	47059.0
19	45071.5
20	40976.0
21	36874.0
22	34318.5
23	35452.0
24	40582.0
25	49821.0
26	63348.5
27	78080.5
28	94043.0
29	112047.0
30	129440.0
31	145981.0
32	164667.5
33	184544.0
34	203452.5
35	221967.5
36	242579.0
37	261197.0
38	275310.5
39	289827.5
40	305217.0
41	317649.0
42	325664.0
43	333926.5
44	344110.0
45	357912.5
46	371261.0
47	386193.0
48	395046.5
49	394318.5
50	393697.0
51	390015.5
52	372728.5
53	348226.0
54	330359.5
55	313037.5
56	293820.0
57	270566.0
58	243248.5
59	213716.5
60	182919.5
61	156681.0
62	130985.0
63	106648.0
64	87185.0
65	69358.5
66	55218.5
67	41443.0
68	28776.0
69	21776.0
70	16122.5
71	10594.5
72	7498.5
73	5430.0
74	3947.0
75	2773.5
76	1827.0
77	1089.5
78	722.0
79	508.0
80	338.0
81	216.5
82	157.0
83	104.0
84	66.5
85	40.5
86	24.5
87	23.0
88	19.0
89	12.5
90	7.5
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	1.6585121310764153E-5
14-15	5.528373770254718E-6
16-17	9.453519147135568E-4
18-19	0.0016806256261574339
20-21	0.0
22-23	0.0032290385222636954
24-25	2.709919638715726E-4
26-27	0.0019087923679288578
28-29	0.0052205181754582395
30-31	0.0018069779502129907
32-33	0.005433205653000488
34-35	0.011211417636395785
36-37	6.770934203698687E-4
38-39	3.939725797335777E-5
40-41	0.0026511875082389333
42-43	0.0056537687902948695
44-45	0.001790325896304209
46-47	0.0018424760832655045
48-49	0.0029916599559627654
50-51	0.010300114940053449
52-53	0.006808340562972262
54-55	0.010236994841251484
56-57	0.018477751908251964
58-59	0.012550003251896808
60-61	0.016637210956884856
62-63	0.015069252517324836
64-65	0.011297934224365832
66-67	0.013739541540647103
68-69	0.00888215520703569
70-71	0.008933982135524052
72-73	0.006672144963382524
74-75	0.010925820081432988
76-77	0.012052492841706347
78-79	0.006812206421725354
80-81	0.0039043638364715913
82-83	0.00225274235102411
84-85	4.1974329759149195E-5
86-87	0.0038271174803165674
88-89	5.606220374629208E-4
90-91	6.205997651358442E-4
92-93	0.0017755383707214168
94-95	0.0013795362772182696
96-97	4.6771612811493096E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	230.0
20-21	727.0
22-23	1638.0
24-25	3469.0
26-27	4764.0
28-29	8261.0
30-31	19270.0
32-33	25187.0
34-35	34231.0
36-37	48836.0
38-39	56653.0
40-41	60401.0
42-43	74277.0
44-45	83248.0
46-47	80635.0
48-49	73966.0
50-51	66037.0
52-53	62709.0
54-55	68398.0
56-57	72796.0
58-59	44636.0
60-61	73087.0
62-63	73310.0
64-65	78817.0
66-67	82800.0
68-69	84771.0
70-71	82589.0
72-73	79849.0
74-75	82578.0
76-77	81045.0
78-79	87489.0
80-81	89874.0
82-83	87801.0
84-85	91099.0
86-87	96474.0
88-89	106236.0
90-91	114474.0
92-93	128605.0
94-95	158399.0
96-97	390188.0
98-99	622779.0
100-101	5461618.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.05830281173039
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.50460318023896	76.98167943160263
2	6.537075273704722	11.120650562677028
3	1.5676903601574328	4.0003524410790465
4	0.5957251617421667	2.0268548480012907
5	0.2822153407910345	1.2002378957559747
6	0.1567839774620747	0.8001467418597996
7	0.08771464210012762	0.5222601012142647
8	0.05957438282550028	0.4053836715354682
9	0.04115747290183903	0.3150706313745191
>10	0.1622174740229274	2.2211133480914915
>50	0.004121732348671128	0.23154580286753268
>100	0.0010744922757052505	0.14663661301538713
>500	4.6509428843871E-5	0.028067910925583403
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1056747540509436E-5
2	0.0	3.3170242621528305E-5	0.0	0.0	1.1056747540509436E-5
3	0.0	3.3170242621528305E-5	0.0	0.0	1.1056747540509436E-5
4	0.0	9.951072786458492E-5	0.0	0.0	2.211349508101887E-5
5	0.0	1.1056747540509435E-4	0.0	0.0	2.211349508101887E-5
6	0.0	1.1056747540509435E-4	0.0	0.0	2.211349508101887E-5
7	0.0	1.1056747540509435E-4	0.0	1.1056747540509436E-5	2.211349508101887E-5
8	0.0	1.4373771802662265E-4	0.0	1.1056747540509436E-5	3.3170242621528305E-5
9	0.0	2.4324844589120758E-4	0.0	1.1056747540509436E-5	3.3170242621528305E-5
10-11	0.0	2.54305193431717E-4	0.0	1.1056747540509436E-5	3.3170242621528305E-5
12-13	0.0	2.54305193431717E-4	0.0	1.1056747540509436E-5	3.869861639178302E-5
14-15	0.0	3.5934429506655663E-4	0.0	2.211349508101887E-5	5.5283737702547173E-5
16-17	0.0	3.759294163773208E-4	0.0	5.5283737702547173E-5	5.5283737702547173E-5
18-19	0.0	4.0357128522859435E-4	0.0	6.634048524305661E-5	7.186885901331133E-5
20-21	0.0	4.422699016203774E-4	0.0	8.845398032407549E-5	8.845398032407547E-5
22-23	0.0	4.975536393229246E-4	0.0	8.845398032407549E-5	1.1056747540509436E-4
24-25	0.0	5.47309003255217E-4	0.0	9.39823540943302E-5	1.3268097048611322E-4
26-27	0.0	5.860076196470001E-4	0.0	1.3820934425636794E-4	1.3268097048611322E-4
28-29	0.0	5.915359934172547E-4	0.0	5.141387606336888E-4	1.3268097048611322E-4
30-31	0.0	7.463304589843869E-4	0.0	0.0017469661114004908	1.3820934425636794E-4
32-33	0.0	7.960858229166793E-4	0.0	0.004660419088324727	1.5479446556713208E-4
34-35	0.0	9.01124924551519E-4	0.0	0.008408656504557426	1.5479446556713208E-4
36-37	0.0	0.0010338058950376322	0.0	0.014821570078052898	1.5479446556713208E-4
38-39	0.0	0.001094618006510434	0.0	0.03268374572974589	1.5479446556713208E-4
40-41	0.0	0.0012051854819155284	0.0	0.052154678148583	1.5479446556713208E-4
42-43	0.0	0.0012936394622396039	0.0	0.06449400840379153	1.6032283933738683E-4
44-45	0.0	0.0014539623015769909	0.0	0.0806976719244081	1.7137958687789626E-4
46-47	0.0	0.0015534730294415756	0.0	0.09734913372041532	1.879647081886604E-4
48-49	0.0	0.00157005815075234	0.0	0.11276776816565573	2.1007820326967926E-4
50-51	0.0	0.0017690796064815098	0.0	0.12768332059780296	2.1007820326967926E-4
52-53	0.0	0.0018354200917245662	0.0	0.1679298816452573	2.1560657703993398E-4
54-55	0.0	0.0018464768392650757	0.0	0.2166680248038229	2.4324844589120758E-4
56-57	0.0	0.0018685903343460945	0.0	0.26611932817875134	2.6536194097222644E-4
58-59	0.0	0.0018685903343460945	0.0	0.4661524763078778	2.8194706228299056E-4
60-61	0.0	0.001879647081886604	0.0	0.644453587146133	2.9853218359375474E-4
62-63	0.0	0.001940459193359406	0.0	0.6520440443326927	3.206456786747736E-4
64-65	0.0	0.0021671225179398495	0.0	0.6586006956242147	3.206456786747736E-4
66-67	0.0	0.0021781792654803588	0.0	0.6645492258010088	3.206456786747736E-4
68-69	0.0	0.0022389913769531606	0.0	0.6709621393745043	3.2617405244502834E-4
70-71	0.0	0.002332973731047491	0.0	0.6762362079513273	3.4828754752604725E-4
72-73	0.0	0.0023882574687500383	0.0	0.6803050910462348	3.9804291145833967E-4
74-75	0.0	0.002482239822844368	0.0	0.683826665137887	3.9804291145833967E-4
76-77	0.0	0.0025762221769386985	0.0	0.6871049907836481	3.9804291145833967E-4
78-79	0.0	0.0025983356720197174	0.0	0.6892555281802772	4.0357128522859435E-4
80-81	0.0	0.0026093924195602267	0.0	0.6898415357999241	4.1462803276910384E-4
82-83	0.0	0.002625977540870991	0.0	0.6903612029343281	4.3121315407986796E-4
84-85	0.0	0.0026757329048032836	0.0	0.6906486783703814	4.422699016203774E-4
86-87	0.0	0.002719959894965321	0.0	0.6906984337343136	4.477982753906321E-4
88	0.0	0.002874754360532453	0.0	0.6907039621080839	4.533266491608868E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10710	0.0	51.647808	1
GTATCAA	19870	0.0	37.735012	1
TCAACGC	23990	0.0	30.708004	4
ATCAACG	24325	0.0	30.285095	3
CAACGCA	24640	0.0	29.91509	5
TATCAAC	25220	0.0	29.394798	2
AACGCAG	25495	0.0	28.994795	6
ACGCAGA	29050	0.0	25.46111	7
GACCGAG	7110	0.0	25.414288	90-91
GAGACCG	6905	0.0	25.409681	88-89
AGACCGA	7150	0.0	25.389359	88-89
CGAGACC	7060	0.0	25.29368	86-87
ACCGAGG	7620	0.0	24.221195	90-91
ACGAGAC	7485	0.0	23.516136	86-87
CGCAGAG	31825	0.0	23.227724	8
CACGAGA	7630	0.0	23.22585	84-85
CCCACGA	7655	0.0	22.654888	82-83
GCCCACG	7635	0.0	22.21027	82-83
TCTCCGA	7645	0.0	22.087606	74-75
ATCTCCG	7805	0.0	21.06636	74-75
>>END_MODULE