FastQCFastQC Report
Fri 27 May 2016
SRR2139199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8684308
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104950.046.6244851
GTATCAA186850.035.613951
TCAACGC220900.029.375074
ATCAACG221150.029.245913
CAACGCA223900.028.905665
AACGCAG230750.028.029186
TATCAAC235300.027.7937972
CGAGACC57100.027.70097286-87
AGACCGA59500.026.78895888-89
GACCGAG60350.026.7655490-91
ACCGAGG61900.026.47766990-91
GAGACCG59550.026.28331688-89
ACGAGAC60100.026.08366286-87
CCCACGA62700.024.75707882-83
CACGAGA64300.024.73659184-85
ACGCAGA261700.024.6656557
GCCCACG63350.024.45999582-83
CGCAGAG268450.024.0770728
TCCGAGC64450.023.48493476-77
ATCTCCG62950.023.46771874-75
Produced by FastQC (version 0.11.5)