FastQCFastQC Report
Fri 27 May 2016
SRR2139198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9243641
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105850.051.1622281
GTATCAA191700.037.4020081
TCAACGC237850.029.5583574
ATCAACG238000.029.50423
CAACGCA242950.028.9378685
TATCAAC247300.028.5314222
AACGCAG252100.027.8875666
GAGACCG65850.026.8573688-89
CGAGACC66150.026.57609686-87
GACCGAG68100.026.43379890-91
AGACCGA67950.026.32649688-89
ACGAGAC69600.025.1361386-87
ACCGAGG72300.025.10467790-91
CACGAGA71400.024.58144284-85
ACGCAGA288250.024.3754637
GCCCACG71950.023.93279382-83
TCTCCGA69650.023.36502374-75
ATCTCCG69300.023.21917374-75
CCCACGA75600.022.92284882-83
CGCAGAG312200.022.464918
Produced by FastQC (version 0.11.5)