##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9243641
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.995746805831164	31.0	31.0	34.0	30.0	34.0
2	32.17744360690771	33.0	31.0	34.0	30.0	34.0
3	32.19139254759028	34.0	31.0	34.0	30.0	34.0
4	35.95178728814761	37.0	35.0	37.0	35.0	37.0
5	35.81310762717851	37.0	35.0	37.0	35.0	37.0
6	35.83593791667158	37.0	35.0	37.0	35.0	37.0
7	35.775740100681105	37.0	35.0	37.0	33.0	37.0
8	35.767059322187	37.0	35.0	37.0	33.0	37.0
9	37.53833365012769	39.0	37.0	39.0	35.0	39.0
10-11	37.413768665399274	39.0	37.0	39.0	34.0	39.0
12-13	37.38757384671257	39.0	37.0	39.0	34.0	39.0
14-15	38.615324091448386	40.0	38.0	41.0	34.0	41.0
16-17	38.57148568405026	40.0	38.0	41.0	34.0	41.0
18-19	38.591424688604846	40.0	38.0	41.0	34.0	41.0
20-21	38.57299587550348	40.0	38.0	41.0	34.0	41.0
22-23	38.48732379012848	40.0	38.0	41.0	34.0	41.0
24-25	38.42052320087531	40.0	38.0	41.0	34.0	41.0
26-27	38.243262960154766	40.0	38.0	41.0	33.5	41.0
28-29	38.13284926840615	40.0	38.0	41.0	33.0	41.0
30-31	37.97853294171666	40.0	37.0	41.0	33.0	41.0
32-33	37.79875437387824	40.0	37.0	41.0	33.0	41.0
34-35	37.89330175325119	40.0	37.0	41.0	33.0	41.0
36-37	37.861721339772124	40.0	37.0	41.0	33.0	41.0
38-39	37.8212661368564	40.0	37.0	41.0	33.0	41.0
40-41	37.73757070719888	40.0	37.0	41.0	33.0	41.0
42-43	37.63268080113605	40.0	37.0	41.0	32.0	41.0
44-45	37.6869415579435	40.0	37.0	41.0	32.0	41.0
46-47	37.67863090046221	40.0	37.0	41.0	32.0	41.0
48-49	37.61274518018378	40.0	37.0	41.0	32.0	41.0
50-51	37.507250033230875	40.0	36.0	41.0	32.0	41.0
52-53	37.306788404704236	39.0	36.0	41.0	31.0	41.0
54-55	37.17427866989873	39.0	35.5	41.0	31.0	41.0
56-57	37.00025652115266	39.0	35.0	41.0	31.0	41.0
58-59	36.84714268229077	39.0	35.0	40.0	31.0	41.0
60-61	36.61887929633836	38.0	35.0	40.0	31.0	41.0
62-63	36.39790703131324	38.0	35.0	40.0	30.0	41.0
64-65	36.0903053040862	37.5	34.5	40.0	30.0	41.0
66-67	35.7967049408053	37.0	34.0	40.0	29.5	41.0
68-69	35.493588316403674	36.5	34.0	39.0	29.0	41.0
70-71	35.20244255678146	36.0	34.0	39.0	29.0	41.0
72-73	34.92438811306627	35.5	34.0	39.0	29.0	40.0
74-75	34.56927206422128	35.0	33.5	38.0	29.0	40.0
76-77	33.303262655449714	34.5	31.5	36.0	27.0	39.0
78-79	34.002505870346205	35.0	33.0	37.0	29.0	39.0
80-81	33.932002073453134	35.0	33.0	36.0	29.0	39.0
82-83	33.66305736257095	35.0	33.0	36.0	29.0	37.5
84-85	33.503204127309296	35.0	33.0	36.0	29.0	37.0
86-87	33.28510938723859	35.0	33.0	35.0	29.0	37.0
88-89	33.194390893449935	35.0	33.0	35.0	29.0	36.0
90-91	33.0740622858513	35.0	33.0	35.0	29.0	36.0
92-93	32.95241597386082	35.0	33.0	35.0	29.0	36.0
94-95	32.92362536495989	34.5	32.5	35.0	29.0	35.5
96-97	33.000755125228345	34.0	33.0	35.0	29.0	35.0
98-99	33.15482996017102	34.0	33.0	35.0	29.0	35.0
100	31.910909529787485	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	7.0
20	71.0
21	318.0
22	1540.0
23	5593.0
24	15358.0
25	33058.0
26	59934.0
27	95048.0
28	135149.0
29	179063.0
30	224213.0
31	274263.0
32	337274.0
33	421743.0
34	549707.0
35	754621.0
36	1150721.0
37	1864981.0
38	2135594.0
39	971067.0
40	34314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30956621963142	20.29639619279892	14.43721148408944	23.956826103480218
2	14.905241343751882	21.528475629895187	40.72455864523514	22.841724381117785
3	18.094082191205825	26.356551493075074	30.62210010103162	24.927266214687478
4	12.73159569914063	18.51822241906625	38.63254749941068	30.11763438238244
5	13.383232862461881	37.69727751218378	35.54749692247892	13.371992702875415
6	29.748937675100105	38.00372602094781	18.899652204147692	13.347684099804397
7	26.480701706178333	32.55198898356178	23.308434414534275	17.658874895725614
8	24.617572231548156	36.269582516240085	21.069662917458608	18.043182334753155
9	25.694680267223706	16.683393480988716	21.162396938608932	36.45952931317865
10-11	24.271042114249138	26.61987846563924	28.807766333634117	20.301313086477503
12-13	25.20543177766379	24.298002502369062	28.704918803365402	21.791646916601742
14-15	22.265858226211943	25.500292579514934	26.490421901932365	25.743427292340755
16-17	21.886900024043797	28.690207953169494	26.994170798603022	22.428721224183683
18-19	21.973575663252173	27.86383687583684	28.616772469998196	21.54581499091279
20-21	22.893465592214717	26.97104687661942	28.52185359189362	21.613633939272244
22-23	22.662591229236376	26.913830499117914	28.298850215285388	22.12472805636032
24-25	22.400442196545868	27.06285067425233	28.45097554337092	22.08573158583088
26-27	22.27632555403436	27.16620129597181	28.38194287722458	22.175530272769244
28-29	22.30636038586502	27.228862725998503	28.347918296761964	22.11685859137451
30-31	22.636873264269898	26.917848737742283	28.30694798529364	22.138330012694183
32-33	22.41528723101154	27.09638401375419	28.18617595619113	22.30215279904314
34-35	22.566954807123697	27.171050669647567	28.18490048198836	22.077094041240375
36-37	22.622827423695867	27.090448205102096	28.04225826680013	22.24446610440191
38-39	22.314380560145857	27.365636375098035	28.11349035872051	22.2064927060356
40-41	22.549209973859746	27.105827571860203	28.09228242999508	22.252680024284967
42-43	22.384047875882732	27.28156620132513	27.83156805135114	22.502817871440996
44-45	22.4355507765755	27.325328792000924	27.698858037489416	22.54026239393416
46-47	22.519699455027702	27.29570361694446	27.573807215694746	22.61078971233309
48-49	22.61194845678224	27.502574492425442	27.374482554998302	22.510994495794016
50-51	22.603442471072594	27.483152075048185	27.359738279176554	22.553667174702667
52-53	22.755057005909133	27.435341750868453	27.243794151704115	22.5658070915183
54-55	22.80046918685659	27.34511307077697	27.25666308624044	22.597754656126003
56-57	22.760563634008378	27.53619718775581	27.07915980403439	22.62407937420142
58-59	22.607761925760524	27.399261388420303	27.244629133805176	22.748347552013996
60-61	22.78242463670278	27.389303737751213	27.309635284505156	22.51863634104085
62-63	22.55636658280933	27.42386630824088	27.393835099920782	22.62593200902901
64-65	22.690598212349254	27.305968611097104	27.296109703025945	22.707323473527698
66-67	22.709864892650995	27.424478575839956	27.273686337187364	22.591970194321686
68-69	22.651150760087667	27.552809469260026	27.18896455322212	22.607075217430182
70-71	22.638579814365496	27.3914793356284	27.03326289232651	22.936677957679596
72-73	22.638187071010734	27.427849677229165	26.938514916688057	22.995448335072048
74-75	22.57108861884472	27.46200029581438	27.157185110813693	22.809725974527208
76-77	22.649219655948546	27.27369435856521	27.091258829917393	22.985827155568852
78-79	22.545127028414996	27.277128043252812	26.9343401382265	23.243404790105686
80-81	22.774047160478823	27.409922775654877	27.021229373469545	22.79480069039676
82-83	22.921608496202932	27.136143455550986	27.136731038266763	22.805517009979315
84-85	22.719379066493936	27.164200003336468	27.061665079108526	23.054755851061064
86-87	22.543889444689956	27.469339167270135	27.078847446011917	22.90792394202799
88-89	22.95309747914454	27.285924746419443	27.005938224337058	22.75503955009896
90-91	22.95666684376287	27.330086188724	27.027494471456375	22.68575249605675
92-93	22.74733562392833	27.116215926599764	27.134027141965856	23.002421307506054
94-95	22.67017037298946	27.45505105786578	27.236960034532377	22.637818534612386
96-97	22.888611014877963	27.348664437822993	27.34439786836389	22.418326678935152
98-99	23.730867303974076	29.47774981978887	27.580438165686456	19.210944710550603
100	22.62858137617091	30.563284102543854	21.312585751576393	25.495548769708844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2011.0
1	1715.0
2	2127.5
3	2977.0
4	3882.0
5	6204.5
6	8327.5
7	10165.5
8	11725.5
9	12008.5
10	11214.5
11	10166.0
12	10842.5
13	14126.5
14	21561.0
15	32851.5
16	42774.5
17	48665.0
18	50346.0
19	48216.5
20	44247.5
21	40139.0
22	37842.0
23	39429.5
24	45302.0
25	56044.5
26	71112.0
27	87268.0
28	105090.5
29	125229.5
30	144841.0
31	163583.5
32	183389.5
33	203518.5
34	222988.0
35	241262.5
36	260452.5
37	279371.0
38	294267.5
39	308808.5
40	324726.0
41	337401.5
42	345880.0
43	353952.5
44	362607.0
45	374252.0
46	385710.0
47	397072.5
48	401804.5
49	397379.0
50	392399.5
51	385211.5
52	366471.5
53	340222.5
54	319537.5
55	300798.5
56	279511.0
57	253883.0
58	226572.0
59	198414.0
60	168537.0
61	143068.0
62	118637.5
63	96622.0
64	79592.5
65	63021.0
66	50026.5
67	37830.5
68	25946.5
69	19009.0
70	14015.0
71	9252.0
72	6545.0
73	4730.0
74	3422.0
75	2391.5
76	1514.5
77	880.0
78	593.0
79	435.5
80	303.0
81	193.0
82	130.5
83	78.5
84	50.0
85	40.0
86	30.0
87	20.5
88	14.5
89	12.5
90	10.5
91	9.0
92	8.0
93	4.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.409123958838298E-6
14-15	0.0
16-17	0.0010115061803027616
18-19	0.0016714193032810341
20-21	0.0
22-23	0.0030998706926539336
24-25	2.7596624575681604E-4
26-27	0.0017701113611160342
28-29	0.005535202043540267
30-31	0.0017569497530265492
32-33	0.0056088486761975784
34-35	0.010984174394019726
36-37	7.554468952856993E-4
38-39	3.853922676657209E-5
40-41	0.0026376732441519015
42-43	0.005876207887176809
44-45	0.001706123830242368
46-47	0.0019672061056619375
48-49	0.002961044872397042
50-51	0.01015660148424118
52-53	0.005860502203636299
54-55	0.009271932924792461
56-57	0.01733604168992161
58-59	0.011318367909246856
60-61	0.01576410220042346
62-63	0.01334609610620218
64-65	0.010108795606761487
66-67	0.012266317384566539
68-69	0.007980171210264772
70-71	0.008476693920215942
72-73	0.006021826441648534
74-75	0.010624461765927377
76-77	0.01155569581419849
78-79	0.007065661681750075
80-81	0.0037756523389999466
82-83	0.0021274094008938227
84-85	2.7348108604808892E-5
86-87	0.003823992964961348
88-89	6.60540087088697E-4
90-91	4.42738552173525E-4
92-93	0.0011849770212600144
94-95	0.0011577949883508013
96-97	4.3505080706504194E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	200.0
20-21	791.0
22-23	1587.0
24-25	3372.0
26-27	4064.0
28-29	8541.0
30-31	19623.0
32-33	25266.0
34-35	35150.0
36-37	49483.0
38-39	57373.0
40-41	61325.0
42-43	75995.0
44-45	85386.0
46-47	83324.0
48-49	74964.0
50-51	67114.0
52-53	63526.0
54-55	69091.0
56-57	73664.0
58-59	45580.0
60-61	74250.0
62-63	73551.0
64-65	78441.0
66-67	83380.0
68-69	85517.0
70-71	84538.0
72-73	81968.0
74-75	84948.0
76-77	83098.0
78-79	90065.0
80-81	92653.0
82-83	89660.0
84-85	93774.0
86-87	100429.0
88-89	110314.0
90-91	121030.0
92-93	136537.0
94-95	161299.0
96-97	396074.0
98-99	644641.0
100-101	5572055.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.14451361739445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.49544645824626	77.0519077327634
2	6.562859833325152	11.175830168952091
3	1.5693164931601495	4.008560685656283
4	0.5679407693273786	1.934281622714739
5	0.29064422317457494	1.2373380508952316
6	0.15337881258259248	0.7835618637935002
7	0.0878319137095347	0.5234883901018306
8	0.0581463613087723	0.3960674921805361
9	0.04394268467652514	0.33673306624427396
>10	0.1664405980522489	2.2343068453509405
>50	0.0033153799169064833	0.18581454447610524
>100	6.919727093350725E-4	0.10208240938599628
>500	4.44998105689062E-5	0.03002712748504764
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.163649583535319E-5	0.0	1.0818247917676596E-5	0.0
3	0.0	2.163649583535319E-5	0.0	1.0818247917676596E-5	0.0
4	0.0	8.654598334141276E-5	0.0	1.0818247917676596E-5	0.0
5	0.0	8.654598334141276E-5	0.0	1.0818247917676596E-5	0.0
6	2.163649583535319E-5	8.654598334141276E-5	0.0	1.0818247917676596E-5	0.0
7	2.163649583535319E-5	9.736423125908935E-5	0.0	1.0818247917676596E-5	0.0
8	2.163649583535319E-5	1.5145547084747233E-4	0.0	4.327299167070638E-5	0.0
9	2.163649583535319E-5	2.5963795002423827E-4	0.0	4.327299167070638E-5	1.0818247917676596E-5
10-11	2.163649583535319E-5	2.5963795002423827E-4	0.0	4.327299167070638E-5	1.0818247917676596E-5
12-13	2.163649583535319E-5	2.704561979419149E-4	0.0	5.4091239588382975E-5	1.6227371876514892E-5
14-15	2.163649583535319E-5	3.4077480940681275E-4	0.0	5.4091239588382975E-5	4.327299167070638E-5
16-17	3.2454743753029783E-5	3.4618393336565106E-4	0.0	6.490948750605957E-5	6.490948750605957E-5
18-19	3.2454743753029783E-5	3.6241130524216594E-4	0.0	1.0277335521792765E-4	8.113685938257445E-5
20-21	3.2454743753029783E-5	3.894569250363574E-4	0.0	1.6227371876514893E-4	9.736423125908935E-5
22-23	4.327299167070638E-5	4.6518466046009357E-4	0.0	4.3272991670706385E-4	9.736423125908935E-5
24-25	5.4091239588382975E-5	4.922302802542851E-4	0.0	0.0013793266095037658	1.0818247917676595E-4
26-27	5.4091239588382975E-5	5.138667760896383E-4	0.0	0.004175843696223165	1.0818247917676595E-4
28-29	5.4091239588382975E-5	5.192759000484765E-4	0.0	0.012695213931393484	1.0818247917676595E-4
30-31	5.4091239588382975E-5	5.517306438015063E-4	0.0	0.014036676673185382	1.0818247917676595E-4
32-33	5.4091239588382975E-5	5.625488917191829E-4	0.0	0.017401151775582803	1.1900072709444255E-4
34-35	5.4091239588382975E-5	6.761404948547871E-4	0.0	0.021257857158234507	1.1900072709444255E-4
36-37	5.4091239588382975E-5	7.951412219492297E-4	0.0	0.028332991296395	1.1900072709444255E-4
38-39	5.4091239588382975E-5	8.167777177845829E-4	0.0	0.046702376260609864	1.2981897501211913E-4
40-41	5.4091239588382975E-5	9.303693209201871E-4	0.0	0.06675940789998228	1.2981897501211913E-4
42-43	5.4091239588382975E-5	9.46596692796702E-4	0.0	0.07990898824391816	1.2981897501211913E-4
44-45	5.950036354722127E-5	0.0010169153042616	0.0	0.0962120878558568	1.4063722292979573E-4
46-47	6.490948750605957E-5	0.0010710065438499828	0.0	0.11388369582938151	1.4063722292979573E-4
48-49	6.490948750605957E-5	0.0010710065438499828	0.0	0.13005697646630804	1.4063722292979573E-4
50-51	8.113685938257447E-5	0.0012224620146974552	0.0	0.1457867089386098	1.4604634688863402E-4
52-53	8.654598334141276E-5	0.001233280262615132	0.0	0.186858187158069	1.5686459480631064E-4
54-55	8.654598334141276E-5	0.001233280262615132	0.0	0.23614071554704474	1.6227371876514893E-4
56-57	8.654598334141276E-5	0.001254916758450485	0.0	0.2841304633098581	1.7309196668282553E-4
58-59	1.0818247917676595E-4	0.001254916758450485	0.0	0.48063852761049464	1.8391021460050213E-4
60-61	1.0818247917676595E-4	0.0012927806261623532	0.0	0.6561970548185504	1.8391021460050213E-4
62-63	1.0818247917676595E-4	0.0013414627417918977	0.0	0.6645433330870378	1.8391021460050213E-4
64-65	1.0818247917676595E-4	0.0015037364605570468	0.0	0.6713696475230919	1.8391021460050213E-4
66-67	1.0818247917676595E-4	0.0015145547084747233	0.0	0.6780607338601747	1.8391021460050213E-4
68-69	1.0818247917676595E-4	0.0015848733199396213	0.0	0.6843461359003449	1.8391021460050213E-4
70-71	1.0818247917676595E-4	0.0016281463116103276	0.0	0.6896470773800064	2.109558343946936E-4
72-73	1.0818247917676595E-4	0.0016335554355691659	0.0	0.6934226459032755	2.271832062712085E-4
74-75	1.0818247917676595E-4	0.001655191931404519	0.0	0.6964030732045954	2.325923302300468E-4
76-77	1.0818247917676595E-4	0.0017363287907870934	0.0	0.6997567300590752	2.380014541888851E-4
78-79	1.0818247917676595E-4	0.001763374410581285	0.0	0.7016553325686274	2.380014541888851E-4
80-81	1.0818247917676595E-4	0.001763374410581285	0.0	0.7023531095593176	2.380014541888851E-4
82-83	1.0818247917676595E-4	0.0017687835345401234	0.0	0.7030508865500078	2.380014541888851E-4
84-85	1.0818247917676595E-4	0.0017850109064166382	0.0	0.703207751144814	2.380014541888851E-4
86-87	1.0818247917676595E-4	0.0017958291543343147	0.0	0.7032726606323201	2.380014541888851E-4
88	1.0818247917676595E-4	0.0018282838980873445	0.0	0.7032834788802378	2.380014541888851E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10585	0.0	51.162228	1
GTATCAA	19170	0.0	37.402008	1
TCAACGC	23785	0.0	29.558357	4
ATCAACG	23800	0.0	29.5042	3
CAACGCA	24295	0.0	28.937868	5
TATCAAC	24730	0.0	28.531422	2
AACGCAG	25210	0.0	27.887566	6
GAGACCG	6585	0.0	26.85736	88-89
CGAGACC	6615	0.0	26.576096	86-87
GACCGAG	6810	0.0	26.433798	90-91
AGACCGA	6795	0.0	26.326496	88-89
ACGAGAC	6960	0.0	25.13613	86-87
ACCGAGG	7230	0.0	25.104677	90-91
CACGAGA	7140	0.0	24.581442	84-85
ACGCAGA	28825	0.0	24.375463	7
GCCCACG	7195	0.0	23.932793	82-83
TCTCCGA	6965	0.0	23.365023	74-75
ATCTCCG	6930	0.0	23.219173	74-75
CCCACGA	7560	0.0	22.922848	82-83
CGCAGAG	31220	0.0	22.46491	8
>>END_MODULE