FastQCFastQC Report
Fri 27 May 2016
SRR2139197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10373189
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122900.053.3051381
GTATCAA228700.038.7643781
TCAACGC281200.030.8998994
ATCAACG284600.030.5458643
CAACGCA290950.029.908775
TATCAAC294700.029.8347032
AACGCAG299900.029.044886
ACGCAGA341950.025.4606157
CGAGACC56300.024.55669286-87
CGCAGAG373700.023.3665148
GAGACCG59550.023.02669188-89
GTACATG332750.022.3381231
ACGAGAC61550.022.28131386-87
GACCGAG63600.022.16915590-91
ACCGAGG65650.021.87708790-91
AGACCGA63700.021.79393488-89
CACGAGA63250.021.73991284-85
TACATGG334350.021.6651542
ACATGGG334850.021.1831913
CCCACGA64150.020.9427282-83
Produced by FastQC (version 0.11.5)