##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139197_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10373189 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.999158889325162 31.0 31.0 34.0 30.0 34.0 2 32.18352080541481 33.0 31.0 34.0 30.0 34.0 3 32.196000092160666 34.0 31.0 34.0 30.0 34.0 4 35.95840276312328 37.0 35.0 37.0 35.0 37.0 5 35.819267825930865 37.0 35.0 37.0 35.0 37.0 6 35.84186087807713 37.0 35.0 37.0 35.0 37.0 7 35.778743258220786 37.0 35.0 37.0 33.0 37.0 8 35.77131294918082 37.0 35.0 37.0 33.0 37.0 9 37.542218791154774 39.0 37.0 39.0 35.0 39.0 10-11 37.41564614314846 39.0 37.0 39.0 34.0 39.0 12-13 37.389873451645386 39.0 37.0 39.0 34.0 39.0 14-15 38.61596761613039 40.0 38.0 41.0 34.0 41.0 16-17 38.572891711507424 40.0 38.0 41.0 34.0 41.0 18-19 38.593815412020355 40.0 38.0 41.0 34.0 41.0 20-21 38.57320396757824 40.0 38.0 41.0 34.0 41.0 22-23 38.48714810687568 40.0 38.0 41.0 34.0 41.0 24-25 38.4231044859595 40.0 38.0 41.0 34.0 41.0 26-27 38.244234239014474 40.0 38.0 41.0 33.5 41.0 28-29 38.13194995767735 40.0 38.0 41.0 33.0 41.0 30-31 37.97672773960443 40.0 37.0 41.0 33.0 41.0 32-33 37.79536071767226 40.0 37.0 41.0 33.0 41.0 34-35 37.88336710780698 40.0 37.0 41.0 33.0 41.0 36-37 37.85187821188005 40.0 37.0 41.0 33.0 41.0 38-39 37.809694854368615 40.0 37.0 41.0 33.0 41.0 40-41 37.721314594818125 40.0 37.0 41.0 33.0 41.0 42-43 37.6144886588818 40.0 37.0 41.0 32.0 41.0 44-45 37.664881000823385 40.0 37.0 41.0 32.0 41.0 46-47 37.65152558508215 40.0 37.0 41.0 32.0 41.0 48-49 37.58159013979939 40.0 36.5 41.0 32.0 41.0 50-51 37.47302685574575 39.5 36.0 41.0 32.0 41.0 52-53 37.26695934892814 39.0 36.0 41.0 31.0 41.0 54-55 37.125543854324974 39.0 35.0 41.0 31.0 41.0 56-57 36.944492562981736 39.0 35.0 41.0 31.0 41.0 58-59 36.78571577578779 39.0 35.0 40.0 31.0 41.0 60-61 36.55348318739655 38.0 35.0 40.0 30.5 41.0 62-63 36.32241413953909 38.0 35.0 40.0 30.0 41.0 64-65 36.00736442519248 37.0 34.0 40.0 30.0 41.0 66-67 35.710751138820285 37.0 34.0 39.5 29.5 41.0 68-69 35.40559666400101 36.0 34.0 39.0 29.0 41.0 70-71 35.11688331995944 36.0 34.0 39.0 29.0 40.5 72-73 34.84464017134874 35.5 34.0 38.5 29.0 40.0 74-75 34.49168341334122 35.0 33.0 37.5 29.0 40.0 76-77 33.2313016967767 34.5 31.5 36.0 27.0 39.0 78-79 33.931134335295255 35.0 33.0 36.5 29.0 39.0 80-81 33.87565534900351 35.0 33.0 36.0 29.0 39.0 82-83 33.61846750719372 35.0 33.0 36.0 29.0 37.0 84-85 33.46711133049245 35.0 33.0 35.5 29.0 37.0 86-87 33.2563171709986 35.0 33.0 35.0 29.0 36.5 88-89 33.17851902329642 35.0 33.0 35.0 29.0 36.0 90-91 33.06381161179565 35.0 33.0 35.0 29.0 36.0 92-93 32.947572492612125 35.0 33.0 35.0 29.0 36.0 94-95 32.92373765118462 34.5 33.0 35.0 29.0 35.0 96-97 33.00296751323029 34.0 33.0 35.0 29.0 35.0 98-99 33.153221028935725 34.0 33.0 35.0 29.0 35.0 100 31.933325839939574 34.0 31.0 35.0 26.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 3.0 19 6.0 20 57.0 21 340.0 22 1846.0 23 6455.0 24 17320.0 25 38069.0 26 68665.0 27 108496.0 28 153760.0 29 203464.0 30 254867.0 31 312464.0 32 382093.0 33 478926.0 34 630102.0 35 868904.0 36 1337935.0 37 2159710.0 38 2357227.0 39 959255.0 40 33224.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.53775097877335 20.175629709979226 14.377739996614347 23.908879314633072 2 15.065849084596838 21.554702223202526 40.32912154593925 23.050327146261388 3 18.05912338047634 26.259600591486382 30.681423041650934 24.999852986386347 4 12.930277557873241 18.340764671381642 38.244019100010526 30.48493867073459 5 13.555079349272436 37.63929298887738 35.29115299065697 13.514474671193208 6 30.161775708511623 37.67857695449297 18.658900363234487 13.500746973760913 7 26.701778980408047 32.56444088698277 23.105459661440662 17.62832047116851 8 25.032745474896874 35.810607519057065 20.978418497918046 18.178228508128022 9 26.054649153698055 16.403046353440587 20.995828765869394 36.546475726991964 10-11 24.5279489267958 26.40387155772444 28.621453826783643 20.44672568869612 12-13 25.386979653071922 24.14901880448994 28.53822414759205 21.925777394846087 14-15 22.52323755018828 25.262096352433183 26.352604777566473 25.862061319812064 16-17 22.146797869660105 28.40835271160614 26.83366228556949 22.61118713316427 18-19 22.251574062318127 27.556533543185814 28.470680760562928 21.72121163393313 20-21 23.182888574272965 26.706536048623764 28.374583170186106 21.735992206917164 22-23 22.9232687828085 26.594966966238466 28.181774442481455 22.29998980847158 24-25 22.677018295402934 26.764782339063242 28.310240641276735 22.24795872425709 26-27 22.553210744209697 26.86200520420155 28.24944817335092 22.335335878237835 28-29 22.621726830630095 26.92519727680494 28.19417756138396 22.258898331181 30-31 22.938751994391477 26.63178988636818 28.096843645263803 22.332614473976545 32-33 22.71935015748142 26.79783101946053 27.991728414835432 22.491090408222618 34-35 22.86822880251151 26.861816365922852 27.995050031932696 22.27490479963294 36-37 22.943858278986788 26.780715584159083 27.842349924331554 22.433076212522572 38-39 22.621484248784228 27.069947824528008 27.919482426504626 22.389085500183135 40-41 22.85688641169122 26.791927657719032 27.883006361879147 22.4681795687106 42-43 22.677484891031813 26.976349482371276 27.61403442338008 22.73213120321683 44-45 22.737680799347157 27.009171813920734 27.474768177448045 22.778379209284065 46-47 22.88117363405498 26.985662147184108 27.305423359062043 22.82774085969887 48-49 22.929864383921046 27.201970655322604 27.103695870579607 22.76446909017674 50-51 22.89509131769911 27.214582835773093 27.09826721988688 22.79205862664092 52-53 23.088906661589917 27.122503800164647 26.991769362169183 22.796820176076253 54-55 23.12306277428555 26.993929634794323 27.023196339870864 22.859811251049262 56-57 23.069236101987666 27.217601561528504 26.86978929315386 22.84337304332997 58-59 22.88153195159141 27.171360069407463 27.00293786836999 22.944170110631134 60-61 23.076463163523727 27.12946567418052 27.03160341960246 22.762467742693296 62-63 22.9713725117077 27.127313824021925 27.110701558356887 22.79061210591349 64-65 22.984413973754823 27.069679336090317 27.022521474152533 22.923385216002323 66-67 23.058340484640542 27.144162593298134 26.93682953986255 22.86066738219877 68-69 22.9514087559016 27.242278473151348 26.951457359296548 22.854855411650504 70-71 22.953408658312384 27.080003520055868 26.817727734147233 23.14886008748451 72-73 22.990512173118024 27.095388520923912 26.7039640588766 23.21013524708146 74-75 22.85921856274777 27.178028997623183 26.891298934322894 23.071453505306152 76-77 22.977836860416367 27.021056931103647 26.80007915286667 23.201027055613313 78-79 22.84009843643755 27.037190780401314 26.69496574288508 23.427745040276054 80-81 23.075264241302442 27.104054653327896 26.764858523480566 23.055822581889096 82-83 23.192441080563896 26.88326945501826 26.902916282395278 23.02137318202257 84-85 23.032682873438297 26.881632999806886 26.82119086630533 23.26449326044949 86-87 22.85176436199663 27.185689918074267 26.80674948263821 23.155796237290897 88-89 23.20866218347341 27.00673082406056 26.72331754289471 23.06128944957132 90-91 23.215199139252235 26.98875524719681 26.791092930343385 23.004952683207566 92-93 23.046511645314926 26.850139677907435 26.9088939745376 23.194454702240037 94-95 22.959937229131622 27.163856487135753 26.931973229951012 22.944233053781613 96-97 23.114513483065412 27.063418315056374 27.070877807558315 22.7511903943199 98-99 24.059094440428787 29.179379674209088 27.2187795729064 19.542746312455726 100 22.982023686187723 30.258262579880267 20.933686075631375 25.82602765830063 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2694.0 1 2250.5 2 2713.5 3 3794.0 4 4855.5 5 7557.5 6 10147.0 7 12452.5 8 14472.0 9 15020.0 10 14071.5 11 12491.5 12 12808.5 13 16375.0 14 25185.0 15 38382.0 16 49787.5 17 56310.0 18 57872.5 19 55065.5 20 49624.5 21 43815.5 22 40042.5 23 40603.5 24 46533.5 25 57435.5 26 72737.0 27 89842.0 28 108116.5 29 128920.5 30 148527.0 31 167135.0 32 187684.5 33 208187.0 34 228325.5 35 248436.0 36 271640.5 37 291988.0 38 305978.5 39 321013.5 40 338127.5 41 351819.0 42 361090.5 43 370655.0 44 381054.0 45 395978.0 46 410668.0 47 426133.5 48 436056.0 49 434812.5 50 432511.5 51 428917.5 52 411175.5 53 385284.0 54 365517.5 55 346176.0 56 325646.5 57 299543.0 58 269613.0 59 238005.5 60 203578.5 61 173654.0 62 143223.5 63 115991.5 64 95558.5 65 75639.0 66 60654.0 67 45846.5 68 31282.0 69 23268.0 70 16891.0 71 10725.0 72 7451.5 73 5237.0 74 3737.0 75 2644.0 76 1678.0 77 948.5 78 615.0 79 442.5 80 319.0 81 208.0 82 148.0 83 94.5 84 57.0 85 43.0 86 32.5 87 26.5 88 15.5 89 6.5 90 4.0 91 3.5 92 3.5 93 3.0 94 3.0 95 2.5 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.640236960880592E-6 2 0.0 3 0.0 4 9.640236960880592E-6 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.446035544132089E-5 14-15 0.0 16-17 9.881242884902607E-4 18-19 0.0018123645486455514 20-21 0.0 22-23 0.0032733307856953227 24-25 2.9896172041090263E-4 26-27 0.0018234295941754834 28-29 0.005082130461908708 30-31 0.0016038449767889333 32-33 0.005327325439246692 34-35 0.01093482524112623 36-37 6.517020000199401E-4 38-39 4.8823242675732425E-5 40-41 0.0025258538305554254 42-43 0.005502294397599343 44-45 0.0015788569016452582 46-47 0.0017008551899895267 48-49 0.002725861838407887 50-51 0.010137409312277948 52-53 0.006228219404101719 54-55 0.009795663184357152 56-57 0.017613791940001122 58-59 0.011729316871702882 60-61 0.015655509398975274 62-63 0.014225025905920143 64-65 0.01028802599522591 66-67 0.013056622128780568 68-69 0.008105309188115856 70-71 0.008537696492413335 72-73 0.006010375493255335 74-75 0.01037665709016637 76-77 0.01136401790349366 78-79 0.006745886101642064 80-81 0.003689647508980103 82-83 0.0020203775981228606 84-85 2.9375056253232726E-5 86-87 0.003665553036744075 88-89 6.094482827617975E-4 90-91 5.823118663757314E-4 92-93 0.001391020903925305 94-95 0.0011267157350308334 96-97 3.86059963618756E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 228.0 20-21 881.0 22-23 1887.0 24-25 3979.0 26-27 4652.0 28-29 7795.0 30-31 15589.0 32-33 20825.0 34-35 27845.0 36-37 37766.0 38-39 44853.0 40-41 51055.0 42-43 66641.0 44-45 75540.0 46-47 73446.0 48-49 65553.0 50-51 58491.0 52-53 56475.0 54-55 62826.0 56-57 67493.0 58-59 43156.0 60-61 70939.0 62-63 72629.0 64-65 78047.0 66-67 83465.0 68-69 87762.0 70-71 87877.0 72-73 87101.0 74-75 91120.0 76-77 90065.0 78-79 98362.0 80-81 101488.0 82-83 100743.0 84-85 105958.0 86-87 114089.0 88-89 125696.0 90-91 137530.0 92-93 155874.0 94-95 185090.0 96-97 452732.0 98-99 747243.0 100-101 6512403.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.78718020016707 #Duplication Level Percentage of deduplicated Percentage of total 1 89.4974643431331 74.98740172376121 2 7.1771735773507315 12.02710271706727 3 1.7902105414072862 4.499900796873928 4 0.65935536745258 2.209821079547868 5 0.30656050762675724 1.2842920247388903 6 0.1761736283004835 0.8856654924557918 7 0.11048536827468337 0.6480080222778902 8 0.07142496247843719 0.4787596961576791 9 0.043738545072364375 0.3298256421904191 >10 0.16223740716602575 2.2481206175771997 >50 0.0040132305900544545 0.22887290396997462 >100 0.0010983964031733675 0.13811212536662235 >500 6.412474428599288E-5 0.034117158015217426 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.892071088264178E-5 0.0 9.640236960880594E-6 0.0 3 0.0 2.892071088264178E-5 0.0 2.892071088264178E-5 0.0 4 0.0 1.7352426529585068E-4 0.0 3.8560947843522376E-5 0.0 5 9.640236960880594E-6 1.7352426529585068E-4 0.0 3.8560947843522376E-5 0.0 6 7.712189568704475E-5 1.7352426529585068E-4 0.0 3.8560947843522376E-5 9.640236960880594E-6 7 7.712189568704475E-5 1.8316450225673128E-4 0.0 3.8560947843522376E-5 1.9280473921761188E-5 8 7.712189568704475E-5 2.1208521313937305E-4 0.0 3.8560947843522376E-5 2.892071088264178E-5 9 7.712189568704475E-5 3.4704853059170136E-4 0.0 3.8560947843522376E-5 2.892071088264178E-5 10-11 1.0604260656968653E-4 3.4704853059170136E-4 0.0 5.302130328484326E-5 2.892071088264178E-5 12-13 1.1568284353056711E-4 3.5186864907214166E-4 0.0 5.784142176528356E-5 3.3740829363082075E-5 14-15 1.349633174523283E-4 4.241704262787461E-4 0.0 6.266154024572385E-5 4.820118480440296E-5 16-17 2.0244497617849245E-4 4.338106632396267E-4 0.0 6.748165872616415E-5 4.820118480440296E-5 18-19 2.1690533161981335E-4 4.7237161108314906E-4 0.0 8.194201416748505E-5 5.784142176528356E-5 20-21 2.2172545010025365E-4 5.20572795887552E-4 0.0 1.0604260656968653E-4 7.712189568704475E-5 22-23 2.2172545010025365E-4 6.121550470159177E-4 0.0 1.8798462073717155E-4 7.712189568704475E-5 24-25 2.2172545010025365E-4 6.796367057420818E-4 0.0 5.832343361332759E-4 7.712189568704475E-5 26-27 2.2172545010025365E-4 7.133775351051639E-4 0.0 0.0017786237192824695 7.712189568704475E-5 28-29 2.2172545010025365E-4 7.278378905464848E-4 0.0 0.006227593076728863 8.676213264792534E-5 30-31 2.2172545010025365E-4 8.483408525574922E-4 0.0 0.007018092507521072 9.640236960880593E-5 32-33 2.2172545010025365E-4 8.531609710379325E-4 0.0 0.008729234568077376 1.0122248808924623E-4 34-35 2.3136568706113423E-4 9.543834591271787E-4 0.0 0.0110525316756496 1.0604260656968653E-4 36-37 2.3136568706113423E-4 0.0010604260656968652 0.0 0.014749562550147307 1.1568284353056711E-4 38-39 2.4100592402201483E-4 0.0011279077244230294 0.0 0.025614109605059734 1.1568284353056711E-4 40-41 2.4100592402201483E-4 0.0012628710418753576 0.0 0.03783793007145633 1.1568284353056711E-4 42-43 2.4100592402201483E-4 0.0013062521081993203 0.0 0.0455501196401608 1.205029620110074E-4 44-45 2.458260425024551E-4 0.0014412154256516487 0.0 0.05610617911232505 1.253230804914477E-4 46-47 2.506461609828954E-4 0.0015424379137408948 0.0 0.0674045368304771 1.30143198971888E-4 48-49 2.506461609828954E-4 0.0015472580322213351 0.0 0.07829318447779174 1.446035544132089E-4 50-51 2.651065164242163E-4 0.0016918615866345441 0.0 0.08944693864153058 1.542437913740895E-4 52-53 2.699266349046566E-4 0.0016966817051149844 0.0 0.11890750279398168 1.590639098545298E-4 54-55 2.699266349046566E-4 0.0017256024159976262 0.0 0.15541990028331693 1.7352426529585068E-4 56-57 2.699266349046566E-4 0.001754523126880268 0.0 0.19267941613712042 1.8316450225673125E-4 58-59 2.699266349046566E-4 0.001768983482321589 0.0 0.34536148912354725 1.9280473921761185E-4 60-61 2.747467533850969E-4 0.0018268249040868725 0.0 0.4818768847265773 1.9280473921761185E-4 62-63 3.2776805666994015E-4 0.001870205970410835 0.0 0.48827800206860206 2.0244497617849245E-4 64-65 3.3258817515038045E-4 0.002034089998745805 0.0 0.4941971075625827 2.0244497617849245E-4 66-67 3.5186864907214166E-4 0.002034089998745805 0.0 0.49974988405204995 2.0244497617849245E-4 68-69 3.5668876755258196E-4 0.002067830828108887 0.0 0.5053701422002433 2.0726509465893275E-4 70-71 3.6150888603302226E-4 0.0020726509465893274 0.0 0.5097805506098463 2.1208521313937305E-4 72-73 3.6632900451346256E-4 0.0020871113020306483 0.0 0.513226935323361 2.1208521313937305E-4 74-75 3.6632900451346256E-4 0.0021353124868350513 0.0 0.5161238265301056 2.1208521313937305E-4 76-77 3.7114912299390286E-4 0.0022027941455612155 0.0 0.5188568337085153 2.1208521313937305E-4 78-79 3.7596924147434316E-4 0.0022461752118851782 0.0 0.5208812834703003 2.1208521313937305E-4 80-81 3.7596924147434316E-4 0.002255815448846059 0.0 0.5214741580433944 2.4100592402201483E-4 82-83 3.7596924147434316E-4 0.0022702758042873797 0.0 0.5218549474033491 2.699266349046566E-4 84-85 3.7596924147434316E-4 0.002289556278209141 0.0 0.5220091911947233 2.795668718655372E-4 86-87 3.807893599547834E-4 0.0023425775814939842 0.0 0.5221248740382538 2.892071088264178E-4 88 4.048899523569849E-4 0.0024775408989463127 0.0 0.5221441545121756 2.892071088264178E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12290 0.0 53.305138 1 GTATCAA 22870 0.0 38.764378 1 TCAACGC 28120 0.0 30.899899 4 ATCAACG 28460 0.0 30.545864 3 CAACGCA 29095 0.0 29.90877 5 TATCAAC 29470 0.0 29.834703 2 AACGCAG 29990 0.0 29.04488 6 ACGCAGA 34195 0.0 25.460615 7 CGAGACC 5630 0.0 24.556692 86-87 CGCAGAG 37370 0.0 23.366514 8 GAGACCG 5955 0.0 23.026691 88-89 GTACATG 33275 0.0 22.338123 1 ACGAGAC 6155 0.0 22.281313 86-87 GACCGAG 6360 0.0 22.169155 90-91 ACCGAGG 6565 0.0 21.877087 90-91 AGACCGA 6370 0.0 21.793934 88-89 CACGAGA 6325 0.0 21.739912 84-85 TACATGG 33435 0.0 21.665154 2 ACATGGG 33485 0.0 21.183191 3 CCCACGA 6415 0.0 20.94272 82-83 >>END_MODULE