FastQCFastQC Report
Fri 27 May 2016
SRR2139196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23408977
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA312500.053.4826131
GTATCAA560600.034.9167021
TCAACGC706200.027.2263264
ATCAACG715500.026.8609033
CAACGCA722550.026.6395195
TATCAAC737700.026.2077622
AACGCAG736900.026.1658446
ACGCAGA851250.022.636567
CGCAGAG914100.021.107398
GTACATG738050.020.7154671
TACATGG740450.020.3006322
GTGGTAT134250.020.135551
ACATGGG741100.019.8318063
TGGTATC126650.019.409452
GCAGAGT994350.019.099349
GAGTACT592100.019.06912812-13
AGAGTAC911800.018.22459810-11
CAGAGTA976250.018.15380310-11
CATGGGG531050.017.7476124
GTACTTT678300.016.6009614-15
Produced by FastQC (version 0.11.5)