##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23408977
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426439352732075	31.0	31.0	34.0	30.0	34.0
2	31.531394601310428	31.0	31.0	34.0	30.0	34.0
3	31.60725054324245	31.0	31.0	34.0	30.0	34.0
4	35.41951136950581	37.0	35.0	37.0	33.0	37.0
5	35.2198130230125	37.0	35.0	37.0	32.0	37.0
6	35.23577531816106	37.0	35.0	37.0	32.0	37.0
7	35.14249896524739	37.0	35.0	37.0	32.0	37.0
8	35.07231114798395	37.0	35.0	37.0	32.0	37.0
9	36.61060660617506	39.0	35.0	39.0	32.0	39.0
10-11	36.53566270751601	38.5	35.0	39.0	32.0	39.0
12-13	36.50146599742483	38.5	35.0	39.0	32.0	39.0
14-15	37.58347105471546	39.0	36.0	41.0	32.0	41.0
16-17	37.548393571406386	39.0	36.0	41.0	32.0	41.0
18-19	37.5764107718163	39.0	36.0	41.0	32.0	41.0
20-21	37.58620027491615	39.0	36.0	41.0	32.0	41.0
22-23	37.48771247712361	39.0	36.0	41.0	32.0	41.0
24-25	37.31582052646209	39.0	36.0	41.0	31.0	41.0
26-27	37.1783190804366	39.0	36.0	41.0	31.0	41.0
28-29	37.016347990225704	39.0	36.0	40.5	31.0	41.0
30-31	36.875911574911854	39.0	36.0	40.0	30.0	41.0
32-33	36.70814627112409	38.5	35.0	40.0	30.0	41.0
34-35	36.556511892685606	38.0	35.0	40.0	30.0	41.0
36-37	36.40579070878927	38.0	35.0	40.0	30.0	41.0
38-39	36.282467844490114	38.0	35.0	40.0	30.0	41.0
40-41	36.157005300272644	38.0	35.0	40.0	30.0	41.0
42-43	36.00214894920137	38.0	34.0	40.0	29.0	41.0
44-45	35.84491030469182	38.0	34.0	40.0	29.0	41.0
46-47	35.99427567054167	38.0	34.0	40.0	29.0	41.0
48-49	36.132768118489366	38.0	34.5	40.0	29.0	41.0
50-51	36.344610868490264	38.0	35.0	40.0	30.0	41.0
52-53	36.319428914135	38.0	35.0	40.0	29.0	41.0
54-55	36.21877215299529	38.0	34.0	40.0	29.0	41.0
56-57	36.067568181390314	38.0	34.0	40.0	29.0	41.0
58-59	35.86855344214813	38.0	34.0	40.0	28.0	41.0
60-61	35.69438978348123	38.0	34.0	40.0	28.0	41.0
62-63	35.49148268310676	37.0	34.0	40.0	28.0	41.0
64-65	35.27215260128028	37.0	33.0	40.0	28.0	41.0
66-67	35.02127072694019	36.5	33.0	39.0	28.0	41.0
68-69	34.75532239293098	36.0	33.0	39.0	28.0	41.0
70-71	34.44509466297352	35.5	32.5	39.0	27.0	40.0
72-73	34.19385749293028	35.0	32.0	38.5	27.0	40.0
74-75	33.83812704297998	35.0	32.0	37.5	26.0	40.0
76-77	32.14959155333882	33.5	30.0	36.0	25.0	38.5
78-79	33.19230489133309	34.5	31.5	36.0	26.0	39.0
80-81	33.292788949085306	35.0	32.0	36.0	26.5	39.0
82-83	33.1145912804228	35.0	32.0	36.0	26.5	37.5
84-85	32.91681978893921	34.5	32.0	35.5	26.0	37.0
86-87	32.72785351685099	34.0	32.0	35.0	26.0	37.0
88-89	32.56550569258493	34.0	31.0	35.0	26.0	36.0
90-91	32.46156542927649	34.0	31.0	35.0	26.0	36.0
92-93	32.40478673414116	34.0	31.0	35.0	26.5	36.0
94-95	32.42651407751366	34.0	31.0	35.0	27.0	35.5
96-97	32.51182085874882	34.0	32.0	35.0	27.0	35.0
98-99	32.64893234155556	34.0	32.0	35.0	28.0	35.0
100	31.598003766244354	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	4.0
19	45.0
20	385.0
21	2275.0
22	10582.0
23	35146.0
24	86639.0
25	172529.0
26	289343.0
27	428452.0
28	575707.0
29	725594.0
30	874363.0
31	1036870.0
32	1224589.0
33	1461429.0
34	1789226.0
35	2249377.0
36	2953416.0
37	3879926.0
38	3828350.0
39	1727457.0
40	57271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.27348140843575	21.46961398612165	14.913253150703682	23.34365145473892
2	14.78328130677498	22.474673535252858	40.79921893665517	21.942826221316988
3	17.767721320264258	27.361116154291416	31.53293078074839	23.33823174469594
4	12.474533251290659	19.083640466494963	39.19235441341339	29.249471868800985
5	12.413203703861129	38.630158848889465	36.38029120196069	12.57634624528872
6	28.978250523292836	39.518395015724096	18.758090112182177	12.74526434880089
7	25.34792955710965	33.365370900231994	24.15636104046751	17.13033850219085
8	24.096601914726985	37.39931480132601	21.247032708862072	17.257050575084936
9	25.356729025729468	16.287164248940325	21.503610376207636	36.85249634912257
10-11	23.757031886314532	27.184881825691836	29.545151239755636	19.512935048237996
12-13	24.560279585855156	24.966652984470585	29.48978751187082	20.983279917803443
14-15	21.71003509277959	26.24273800617213	27.13968567530367	24.90754122574461
16-17	21.162286734850454	29.38012689880869	27.687214360630886	21.770372005709966
18-19	21.253782863526766	28.41074578020778	29.43169708350524	20.903774272760213
20-21	22.185115644222382	27.498311897657302	29.4032663154229	20.913306142697415
22-23	21.94164330332947	27.43354161072598	29.194423359565562	21.430391726378986
24-25	21.69302789542293	27.588399272512977	29.32059951315331	21.397973318910786
26-27	21.58211878319008	27.65863151765375	29.244400249820252	21.514849449335923
28-29	21.683695573069397	27.681435231363956	29.20353349629484	21.431335699271813
30-31	21.977610211495467	27.46574316134085	29.099504051191605	21.457142575972078
32-33	21.79627265516523	27.651211788145186	28.94295134093372	21.609564215755864
34-35	21.919440217737026	27.696008767345386	28.943333167324358	21.44121784759323
36-37	21.992730065340226	27.581638194411518	28.81359571565157	21.612036024596684
38-39	21.676913038003814	27.857415819306265	28.882612611489478	21.58305853120044
40-41	21.899993884413657	27.649107014401547	28.8319362078349	21.618962893349895
42-43	21.765594755870143	27.82716023423037	28.492813063709598	21.914431946189882
44-45	21.829357900103854	27.85386642580192	28.32595640240542	21.990819271688807
46-47	21.968488488552918	27.84202104310444	28.13968866148225	22.04980180686039
48-49	22.016834962004168	28.08593196098373	27.936048023693022	21.96118505331908
50-51	21.991887094629366	28.07681589278384	27.90053133979729	22.030765672789503
52-53	22.168401124874464	27.94587833661419	27.823816528221585	22.06190401028977
54-55	22.203027179669128	27.860441420859406	27.84724299990983	22.089288399561642
56-57	22.169335781489046	28.04414038994041	27.683247897129988	22.103275931440564
58-59	22.069215560988493	28.018268036061894	27.800044084144933	22.11247231880468
60-61	22.12046177143265	28.015956727675917	27.75640431821605	22.107177182675382
62-63	22.076915728286618	28.02886789356235	27.890662087649638	22.0035542905014
64-65	22.1362081171868	27.978349823332316	27.740296736528176	22.145145322952704
66-67	22.179146035564614	28.145299789736722	27.477091010288348	22.198463164410317
68-69	22.155087746120618	28.055310260412835	27.57910520900288	22.21049678446366
70-71	22.136633314254357	27.83932100628449	27.654313684819382	22.36973199464177
72-73	22.195785093353884	27.835451337767648	27.533572128223415	22.435191440655053
74-75	22.10498264113229	27.90126451857471	27.55081905371831	22.44293378657469
76-77	22.209525190332872	27.915996467931176	27.512884801965804	22.361593539770148
78-79	22.130227214053377	27.93690493761027	27.524355629179027	22.408512219157327
80-81	22.184926851506713	27.876247649423895	27.52198485260962	22.41684064645977
82-83	22.31411146841837	27.76226871636983	27.73035813968876	22.193261675523036
84-85	22.300975751388215	27.782507890676882	27.655030135358082	22.261486222576817
86-87	22.144678099555925	27.93555828728899	27.659763263405623	22.26000034974946
88-89	22.34480366592094	27.730790646304243	27.579886464550874	22.344519223223944
90-91	22.356675103787595	27.732205769693465	27.708360995166604	22.202758131352336
92-93	22.364622031348848	27.72395766785123	27.803735223066212	22.107685077733713
94-95	22.060181796988605	27.808748727105748	27.913444916604348	22.217624559301303
96-97	22.061981122033426	27.90676710617231	28.02711435414271	22.00413741765155
98-99	23.17087638018154	29.87884391131896	28.19289263531946	18.75738707318004
100	22.298159145107167	30.649287917822765	22.083056939347024	24.969495997723044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8321.0
1	6744.0
2	7588.5
3	10502.5
4	13147.0
5	20635.0
6	28319.5
7	35673.0
8	42044.5
9	42977.0
10	38561.0
11	33188.0
12	34654.5
13	44272.0
14	69024.5
15	104907.0
16	134097.0
17	150704.5
18	157061.5
19	155244.5
20	147521.0
21	137373.5
22	131770.5
23	138420.5
24	159347.5
25	196735.0
26	248010.5
27	304880.5
28	369755.5
29	443916.5
30	513980.5
31	575889.0
32	633377.5
33	683536.0
34	729031.5
35	767429.0
36	803703.0
37	828872.5
38	833640.0
39	835512.0
40	838929.0
41	837801.0
42	831137.5
43	829792.0
44	836251.5
45	856084.0
46	877500.0
47	900607.0
48	912916.0
49	901629.5
50	890788.0
51	877188.5
52	837429.5
53	779412.0
54	734028.0
55	695599.0
56	653066.0
57	599663.5
58	539913.0
59	475745.0
60	406797.5
61	347851.5
62	288817.0
63	233519.0
64	189982.0
65	149940.5
66	118479.0
67	88995.5
68	61462.0
69	44799.5
70	32928.5
71	22607.0
72	15768.5
73	11184.5
74	8335.5
75	6047.0
76	4055.5
77	2555.5
78	1903.5
79	1522.5
80	1043.5
81	656.5
82	488.5
83	311.5
84	189.0
85	140.5
86	104.0
87	77.5
88	60.5
89	43.5
90	26.0
91	17.5
92	13.5
93	5.5
94	1.0
95	2.0
96	1.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.10443002272162512
3	1.1534036707370851E-4
4	8.970917439066218E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.009718493892321736
10-11	0.0010722382272407719
12-13	8.99227676630209E-4
14-15	1.3029189613881888E-4
16-17	3.801960247985207E-4
18-19	5.254394500024498E-4
20-21	1.3030161491549343E-4
22-23	2.136499728482932E-6
24-25	0.15975026106734683
26-27	0.001586909154369903
28-29	0.0016096113661850447
30-31	0.0023240460750281397
32-33	0.001982218127453573
34-35	0.0013939380783359538
36-37	2.928023393995978E-4
38-39	3.2094745435177455E-4
40-41	7.589429047252775E-4
42-43	9.005298664500899E-4
44-45	8.512697741567847E-5
46-47	0.0
48-49	4.58080471958478E-6
50-51	0.0
52-53	0.0
54-55	1.834417223051682E-4
56-57	0.0
58-59	7.914131386668056E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.004101197036885141
70-71	0.006532117896498592
72-73	0.0
74-75	2.0062674210337764E-4
76-77	0.0014765777058915075
78-79	0.010026404128797345
80-81	5.498581393493385E-5
82-83	6.421196783773289E-5
84-85	1.4741223769905828E-4
86-87	4.033352947912848E-5
88-89	3.8121171663008254E-5
90-91	0.0
92-93	1.2235819854838516E-5
94-95	1.8825731800906216E-5
96-97	0.0
98-99	3.5949013514132907E-6
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1077.0
20-21	3518.0
22-23	7510.0
24-25	14120.0
26-27	16420.0
28-29	30544.0
30-31	61118.0
32-33	78046.0
34-35	109837.0
36-37	145898.0
38-39	167469.0
40-41	181491.0
42-43	212017.0
44-45	256342.0
46-47	237500.0
48-49	216866.0
50-51	186012.0
52-53	176107.0
54-55	190761.0
56-57	213214.0
58-59	218920.0
60-61	217921.0
62-63	218913.0
64-65	231649.0
66-67	249274.0
68-69	259326.0
70-71	256170.0
72-73	248211.0
74-75	253971.0
76-77	233706.0
78-79	260070.0
80-81	277227.0
82-83	271986.0
84-85	277240.0
86-87	298321.0
88-89	328421.0
90-91	361026.0
92-93	395713.0
94-95	471929.0
96-97	997725.0
98-99	1536938.0
100-101	1.3038453E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.61032469651076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.4839109476238	74.8177884944448
2	7.203765945136836	12.04618419421115
3	1.7409607461255985	4.366868798023228
4	0.6805136759949812	2.27591877641426
5	0.3267025483687035	1.3657853074142405
6	0.17142237461291734	0.8599608240979754
7	0.10017759463580185	0.5863116850370378
8	0.06966023439190207	0.46594518527535816
9	0.04619529217885702	0.3476163040671977
>10	0.1696304737073859	2.2555576555200783
>50	0.005424952581408734	0.3017824071234165
>100	0.0015324280052345285	0.2066309730979128
>500	5.147739081068188E-5	0.03264219567713431
>1k	5.130924583091178E-5	0.07100719959622541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.27186544717439E-6
2	0.0	2.5631192683046336E-5	0.0	0.0	4.27186544717439E-6
3	0.0	2.5631192683046336E-5	0.0	0.0	4.27186544717439E-6
4	0.0	8.970917439066218E-5	0.0	4.27186544717439E-6	8.54373089434878E-6
5	0.0	8.970917439066218E-5	0.0	8.54373089434878E-6	8.54373089434878E-6
6	3.417492357739512E-5	9.398103983783657E-5	0.0	8.54373089434878E-6	1.2815596341523168E-5
7	3.417492357739512E-5	9.398103983783657E-5	0.0	8.54373089434878E-6	1.2815596341523168E-5
8	3.417492357739512E-5	1.0679663617935973E-4	0.0	8.54373089434878E-6	1.708746178869756E-5
9	3.417492357739512E-5	2.0077767601719632E-4	0.0	1.708746178869756E-5	1.708746178869756E-5
10-11	3.6310856300982314E-5	2.0077767601719632E-4	0.0	2.776712540663353E-5	1.708746178869756E-5
12-13	3.844678902456951E-5	2.0077767601719632E-4	0.0	2.9903058130220728E-5	1.9223394512284754E-5
14-15	3.844678902456951E-5	2.669915904483993E-4	0.0	3.417492357739512E-5	4.05827217481567E-5
16-17	5.9806116260441456E-5	2.712634558955738E-4	0.0	5.126238536609267E-5	5.126238536609267E-5
18-19	6.407798170761584E-5	2.968946485786201E-4	0.0	7.475764532555181E-5	5.126238536609267E-5
20-21	6.407798170761584E-5	3.225258412616664E-4	0.0	1.4951529065110363E-4	5.126238536609267E-5
22-23	6.407798170761584E-5	3.5242889939188715E-4	0.0	5.382550463439731E-4	5.980611626044145E-5
24-25	6.407798170761584E-5	3.844678902456951E-4	0.0	0.0016233088699262681	6.621391443120304E-5
26-27	6.621391443120304E-5	4.0582721748156703E-4	0.0	0.004489730584980284	6.834984715479024E-5
28-29	6.834984715479024E-5	4.079631502051542E-4	0.0	0.015011335181370804	6.834984715479024E-5
30-31	6.834984715479024E-5	4.335943428882005E-4	0.0	0.017636396498659467	6.834984715479024E-5
32-33	7.048577987837744E-5	4.4000214105896214E-4	0.0	0.023439725708645876	7.262171260196463E-5
34-35	7.262171260196463E-5	4.93400459148642E-4	0.0	0.030949665164778453	7.689357804913902E-5
36-37	7.262171260196463E-5	5.660221717506066E-4	0.0	0.04488449025346131	7.689357804913902E-5
38-39	7.262171260196463E-5	5.767018353685426E-4	0.0	0.08190447621867457	7.689357804913902E-5
40-41	7.689357804913902E-5	6.386438843525711E-4	0.0	0.11506910361781295	8.330137621990059E-5
42-43	7.689357804913902E-5	6.600032115884431E-4	0.0	0.13541386280998097	8.970917439066218E-5
44-45	8.11654434963134E-5	7.689357804913902E-4	0.0	0.1641186626822693	1.0252477073218535E-4
46-47	8.543730894348779E-5	8.629168203292267E-4	0.0	0.19662328686981922	1.1106850162653414E-4
48-49	8.543730894348779E-5	8.629168203292267E-4	0.0	0.22742770860939376	1.2815596341523167E-4
50-51	1.0466070345577255E-4	9.718493892321736E-4	0.0	0.2521810329430457	1.2815596341523167E-4
52-53	1.1106850162653414E-4	9.953446491916327E-4	0.0	0.3172671748961947	1.4524342520392923E-4
54-55	1.1106850162653414E-4	0.0010060243128095687	0.0	0.3980267911750266	1.64466819716214E-4
56-57	1.1106850162653414E-4	0.0010252477073218534	0.0	0.48012777320427114	1.6873868516338838E-4
58-59	1.1106850162653414E-4	0.0010273836400454407	0.0	0.5260524626941194	1.7301055061056276E-4
60-61	1.1320443435012132E-4	0.0010444711018341383	0.0	0.5582452407040257	1.7514648333414996E-4
62-63	1.1961223252088291E-4	0.0010572866981756615	0.0	0.5826568158019036	1.8369021422849876E-4
64-65	1.1961223252088291E-4	0.0011448599398427364	0.0	0.5893465570921788	1.8796207967567314E-4
66-67	1.238840979680573E-4	0.0011491318052899108	0.0	0.5954660043452561	1.9650581057002194E-4
68-69	1.238840979680573E-4	0.0011897145270380674	0.0	0.6017029278981307	2.0077767601719632E-4
70-71	1.2815596341523167E-4	0.0012089379215503523	0.0	0.6074122760682793	2.1359327235871947E-4
72-73	1.3883562703316765E-4	0.0012089379215503523	0.0	0.6119126863168775	2.434963304889402E-4
74-75	1.4097155975675485E-4	0.001238840979680573	0.0	0.6152895959528688	2.434963304889402E-4
76-77	1.4737935792751646E-4	0.0012751518359815552	0.0	0.6184635919801194	2.434963304889402E-4
78-79	1.5378715609827803E-4	0.0012858314995994912	0.0	0.6206700104835849	2.434963304889402E-4
80-81	1.559230888218652E-4	0.0012879674323230785	0.0	0.6214731211876539	2.5631192683046335E-4
82-83	1.580590215454524E-4	0.0012986470959410145	0.0	0.6220540948884695	2.7339938861916096E-4
84-85	1.580590215454524E-4	0.0013135986250061248	0.0	0.6223061349498528	2.776712540663353E-4
86-87	1.580590215454524E-4	0.0013328220195184095	0.0	0.6224257471823736	2.819431195135097E-4
88	1.623308869926268E-4	0.0014182593284618973	0.0	0.6224321549805444	2.904868504078585E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	31250	0.0	53.482613	1
GTATCAA	56060	0.0	34.916702	1
TCAACGC	70620	0.0	27.226326	4
ATCAACG	71550	0.0	26.860903	3
CAACGCA	72255	0.0	26.639519	5
TATCAAC	73770	0.0	26.207762	2
AACGCAG	73690	0.0	26.165844	6
ACGCAGA	85125	0.0	22.63656	7
CGCAGAG	91410	0.0	21.10739	8
GTACATG	73805	0.0	20.715467	1
TACATGG	74045	0.0	20.300632	2
GTGGTAT	13425	0.0	20.13555	1
ACATGGG	74110	0.0	19.831806	3
TGGTATC	12665	0.0	19.40945	2
GCAGAGT	99435	0.0	19.09934	9
GAGTACT	59210	0.0	19.069128	12-13
AGAGTAC	91180	0.0	18.224598	10-11
CAGAGTA	97625	0.0	18.153803	10-11
CATGGGG	53105	0.0	17.747612	4
GTACTTT	67830	0.0	16.60096	14-15
>>END_MODULE