FastQCFastQC Report
Fri 27 May 2016
SRR2139195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24150697
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA314250.051.726731
GTATCAA554450.034.789781
TCAACGC703500.026.9032764
ATCAACG714650.026.4892863
CAACGCA717800.026.3740235
AACGCAG730150.025.9449866
TATCAAC736200.025.8792292
ACGCAGA846500.022.3594557
GTACATG728900.021.1148621
CGCAGAG913550.020.7138378
TACATGG739400.020.584552
GTGGTAT138600.020.5114021
ACATGGG737150.020.0421433
TGGTATC136900.019.0052412
GCAGAGT989650.018.7979779
GAGTACT591400.018.45425212-13
AGAGTAC906050.018.23962810-11
CAGAGTA971200.017.85191710-11
TATATAC313050.017.3760743
CATGGGG547850.017.3567664
Produced by FastQC (version 0.11.5)