##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139195_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24150697 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.391200096626612 31.0 31.0 34.0 30.0 34.0 2 31.505088942153513 31.0 31.0 34.0 30.0 34.0 3 31.58177008307462 31.0 31.0 34.0 30.0 34.0 4 35.39617684740114 37.0 35.0 37.0 33.0 37.0 5 35.19340700601726 37.0 35.0 37.0 32.0 37.0 6 35.211197589866664 37.0 35.0 37.0 32.0 37.0 7 35.11633920958886 37.0 35.0 37.0 32.0 37.0 8 35.04693669917684 37.0 35.0 37.0 32.0 37.0 9 36.578596634291756 39.0 35.0 39.0 32.0 39.0 10-11 36.50498670907925 38.5 35.0 39.0 32.0 39.0 12-13 36.467280054070486 38.0 35.0 39.0 32.0 39.0 14-15 37.5431528953388 39.0 36.0 41.0 32.0 41.0 16-17 37.50661873237033 39.0 36.0 41.0 32.0 41.0 18-19 37.531645463482896 39.0 36.0 41.0 32.0 41.0 20-21 37.53792371384966 39.0 36.0 41.0 32.0 41.0 22-23 37.437278410330094 39.0 36.0 41.0 32.0 41.0 24-25 37.26896364994306 39.0 36.0 41.0 31.0 41.0 26-27 37.12288612541589 39.0 36.0 41.0 31.0 41.0 28-29 36.95844792776565 39.0 36.0 40.5 30.5 41.0 30-31 36.820206010419625 39.0 35.5 40.0 30.0 41.0 32-33 36.65416395013943 38.5 35.0 40.0 30.0 41.0 34-35 36.50060990139977 38.0 35.0 40.0 30.0 41.0 36-37 36.34989205422467 38.0 35.0 40.0 30.0 41.0 38-39 36.22284960686699 38.0 35.0 40.0 30.0 41.0 40-41 36.091244508023564 38.0 34.5 40.0 29.5 41.0 42-43 35.934369986344905 38.0 34.0 40.0 29.0 41.0 44-45 35.774506902824015 38.0 34.0 40.0 28.5 41.0 46-47 35.92237266212564 38.0 34.0 40.0 29.0 41.0 48-49 36.05884436070943 38.0 34.0 40.0 29.0 41.0 50-51 36.271016483026045 38.0 35.0 40.0 29.0 41.0 52-53 36.24237536417188 38.0 34.5 40.0 29.0 41.0 54-55 36.13672545146002 38.0 34.0 40.0 29.0 41.0 56-57 35.97910293568043 38.0 34.0 40.0 28.0 41.0 58-59 35.77528017970759 38.0 34.0 40.0 28.0 41.0 60-61 35.59653627522897 37.5 34.0 40.0 28.0 41.0 62-63 35.39063614309085 37.0 33.5 40.0 28.0 41.0 64-65 35.1670126385888 37.0 33.0 40.0 28.0 41.0 66-67 34.909832697797455 36.0 33.0 39.0 28.0 41.0 68-69 34.64221649782504 36.0 33.0 39.0 27.5 41.0 70-71 34.32973875761065 35.0 32.0 39.0 27.0 40.0 72-73 34.07539345063206 35.0 32.0 38.0 26.0 40.0 74-75 33.7179951493392 35.0 32.0 37.5 26.0 39.5 76-77 32.03890632571302 33.5 29.5 35.5 25.0 38.5 78-79 33.08567524691175 34.5 31.0 36.0 26.0 39.0 80-81 33.19286255932063 35.0 32.0 36.0 26.0 39.0 82-83 33.019185188570745 35.0 32.0 36.0 26.0 37.5 84-85 32.82688975165371 34.0 32.0 35.0 26.0 37.0 86-87 32.644120495046025 34.0 31.5 35.0 26.0 37.0 88-89 32.486401644663346 34.0 31.0 35.0 26.0 36.0 90-91 32.38908461024235 34.0 31.0 35.0 26.0 36.0 92-93 32.33368432050256 34.0 31.0 35.0 26.0 36.0 94-95 32.3591648816311 34.0 31.0 35.0 27.0 35.0 96-97 32.45194163831849 34.0 31.0 35.0 27.0 35.0 98-99 32.59588429484921 34.0 31.5 35.0 27.5 35.0 100 31.540329478101082 33.0 30.0 35.0 25.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 4.0 18 9.0 19 72.0 20 438.0 21 2581.0 22 11643.0 23 38023.0 24 93971.0 25 185449.0 26 309764.0 27 457176.0 28 614004.0 29 767779.0 30 922663.0 31 1090126.0 32 1284234.0 33 1531766.0 34 1869574.0 35 2339068.0 36 3062843.0 37 3988974.0 38 3832093.0 39 1691789.0 40 56653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.2562750052307 21.430251060662968 14.881442138088188 23.432031796018144 2 14.959316670103487 22.294000553345512 40.67149203296447 22.07519074358653 3 18.041122327176264 27.110330162186763 31.220894189462854 23.627653321174115 4 12.72098658613006 18.918238058283592 38.76715821678517 29.593617138801175 5 12.63628126343517 38.452285662811306 36.1984004022741 12.713032671479418 6 29.42284440072268 39.13912712332899 18.55869832659488 12.879330149353455 7 25.636928822385542 33.10265124025199 23.953478444121096 17.306941493241375 8 24.194614341772414 37.42831935658006 21.021008213551767 17.35605808809576 9 25.351830909638508 16.26287488879726 21.432102857403628 36.95319134416061 10-11 23.995457556034548 27.02439465325584 29.343036070715993 19.637111719993623 12-13 24.717955498084436 24.78713629333005 29.309367800158576 21.185540408426935 14-15 21.835316374007018 26.062506478859 26.913615565319127 25.18856158181486 16-17 21.380838085301246 29.16444941749604 27.493650936372887 21.961061560829823 18-19 21.465323744462733 28.251073189066883 29.19928486152551 21.084318204944875 20-21 22.359864711477677 27.337051290696916 29.153261170979544 21.14982282684586 22-23 22.149957326182186 27.274565847513948 28.94098293728611 21.634493889017754 24-25 21.92482620086242 27.47079154905182 29.0140868916636 21.590295358422168 26-27 21.79979450807255 27.4685805115025 29.010118244720314 21.721506735704637 28-29 21.906280319506955 27.476055765391926 28.983730221771847 21.633933693329265 30-31 22.180382462330446 27.265433469201888 28.8737818531487 21.680402215318963 32-33 22.006617940383773 27.479417114273826 28.70897523076799 21.804989714574415 34-35 22.114519581548958 27.513838683158625 28.730429316352968 21.64121241893945 36-37 22.21147277430566 27.401396803684918 28.57361050568975 21.813519916319667 38-39 21.926297419209774 27.672053757341974 28.630521569757484 21.77112725369077 40-41 22.081482335217 27.47749146714284 28.60196156543171 21.83906463220845 42-43 21.953797899698245 27.66935302052142 28.244756229310518 22.132092850469817 44-45 22.010115715617577 27.673770341193627 28.138701990373892 22.17741195281491 46-47 22.149679432294274 27.686080463836117 27.968992735969827 22.195247367899785 48-49 22.20140704867174 27.912289609739 27.74440601819426 22.141897323395003 50-51 22.18711793560649 27.893227722304186 27.69528091342419 22.22437342866513 52-53 22.33949988005566 27.78525509957547 27.61836217982145 22.25688284054742 54-55 22.358085578031485 27.695744854172933 27.665519989187036 22.280649578608543 56-57 22.334526640169642 27.860028766025145 27.500530376028394 22.304914217776815 58-59 22.245855424226914 27.828232205487318 27.607826000936807 22.318086369348954 60-61 22.29949151354933 27.873163146870176 27.556761056089655 22.270584283490834 62-63 22.28892260568771 27.873676060537793 27.670838384280284 22.16656294949421 64-65 22.298962103376123 27.796014752591976 27.554081572234544 22.350941571797353 66-67 22.358530138879257 27.92679786387753 27.300745958871676 22.41392603837154 68-69 22.297431928907226 27.887667988302567 27.359124010922486 22.455776071867714 70-71 22.316042649283016 27.69452196648448 27.452178305461477 22.537257078771024 72-73 22.31903038365422 27.714164031525808 27.301005234560105 22.665800350259875 74-75 22.286290503403794 27.740468030999903 27.33283275232367 22.640408713272635 76-77 22.42070723504121 27.746750035490063 27.306838669146888 22.52570406032184 78-79 22.26946325874779 27.73085360576629 27.307797580034997 22.691885555450924 80-81 22.333260839460976 27.73642120570884 27.314835734171535 22.61548222065865 82-83 22.47802154776717 27.61241651557241 27.528421262292824 22.381140674367593 84-85 22.468166619398577 27.665321112375395 27.42786503848983 22.438647229736198 86-87 22.27490027476194 27.84530163761902 27.452178994538023 22.427619093081017 88-89 22.447929173220544 27.65036941836706 27.392543689089322 22.509157719323074 90-91 22.481935354205362 27.653149087013507 27.46732197335802 22.397593585423113 92-93 22.5394102957864 27.56103207402909 27.57630894100297 22.32324868918154 94-95 22.208422662720047 27.663607397459167 27.728664775062867 22.399305164757923 96-97 22.17802381417472 27.751383624875697 27.850152265178973 22.22044029577061 98-99 23.326746162300648 29.728552407977677 28.006215088303964 18.93848634141771 100 22.51550096409908 30.443663898960665 21.818099681254008 25.22273545568625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7373.0 1 6185.0 2 7617.0 3 10666.0 4 13323.0 5 21029.0 6 28810.5 7 36033.5 8 42058.5 9 42736.5 10 38740.5 11 33720.0 12 34852.5 13 43858.5 14 68576.0 15 104731.5 16 133805.5 17 150376.0 18 156562.0 19 154232.5 20 146124.0 21 135636.0 22 129061.0 23 134597.0 24 154409.5 25 189501.5 26 236178.5 27 287483.5 28 348899.0 29 421992.5 30 494376.5 31 560971.0 32 623328.5 33 678144.0 34 723381.0 35 758787.0 36 794251.5 37 824792.0 38 839021.5 39 846823.0 40 854099.5 41 856639.5 42 852049.0 43 854349.0 44 865127.5 45 891054.0 46 919007.5 47 943708.5 48 959501.5 49 950479.5 50 937360.5 51 925809.5 52 890698.5 53 834563.5 54 788913.0 55 747972.0 56 703518.5 57 647999.5 58 583768.0 59 515089.5 60 440719.5 61 376872.0 62 314103.0 63 254113.0 64 206472.5 65 163697.0 66 129928.5 67 97724.5 68 67724.0 69 49349.0 70 35814.5 71 24166.5 72 17007.0 73 12317.5 74 9032.0 75 6435.0 76 4292.0 77 2701.5 78 2066.5 79 1645.5 80 1116.5 81 736.0 82 564.0 83 355.5 84 209.5 85 138.5 86 105.0 87 85.0 88 60.5 89 39.0 90 23.0 91 16.5 92 13.5 93 8.0 94 3.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.10236557561879064 3 8.695401213472223E-5 4 9.523534662374341E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.009258531958725663 10-11 0.0010827844844395174 12-13 8.633291204804565E-4 14-15 1.3457168544659396E-4 16-17 3.664490511391866E-4 18-19 5.362164081641204E-4 20-21 1.345818888555678E-4 22-23 0.0 24-25 0.15122909454114908 26-27 0.001612829799854041 28-29 0.001595471897225219 30-31 0.0022755423708483197 32-33 0.0019317425943615623 34-35 0.0014180127609436284 36-37 3.005931227824951E-4 38-39 4.1258924464049747E-4 40-41 8.504914476370534E-4 42-43 9.390303091693904E-4 44-45 1.041531909434808E-4 46-47 0.0 48-49 4.434736018901554E-6 50-51 0.0 52-53 0.0 54-55 1.8886238469524767E-4 56-57 0.0 58-59 8.581795247550234E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0037578093815869742 70-71 0.006133836586292281 72-73 5.0359422754109495E-6 74-75 2.6528886513759944E-4 76-77 0.0014888545280563779 78-79 0.009719725229233122 80-81 7.703630189623528E-5 82-83 8.362024067307927E-5 84-85 1.69808781891219E-4 86-87 3.061723567778599E-5 88-89 5.383349142435315E-5 90-91 0.0 92-93 1.7739730130216124E-5 94-95 2.1235791397423376E-5 96-97 0.0 98-99 3.4815299959572473E-6 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 1103.0 20-21 3790.0 22-23 7527.0 24-25 14930.0 26-27 17185.0 28-29 31163.0 30-31 62565.0 32-33 80476.0 34-35 111650.0 36-37 148447.0 38-39 169983.0 40-41 183313.0 42-43 214662.0 44-45 258591.0 46-47 239744.0 48-49 219603.0 50-51 188201.0 52-53 176898.0 54-55 192810.0 56-57 216028.0 58-59 222201.0 60-61 225404.0 62-63 223233.0 64-65 236630.0 66-67 254085.0 68-69 266264.0 70-71 263803.0 72-73 254833.0 74-75 262720.0 76-77 241806.0 78-79 268351.0 80-81 285916.0 82-83 280467.0 84-85 286548.0 86-87 309988.0 88-89 341096.0 90-91 378008.0 92-93 413625.0 94-95 497290.0 96-97 1049037.0 98-99 1592044.0 100-101 1.3458679E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.45346327821143 #Duplication Level Percentage of deduplicated Percentage of total 1 89.03509144422237 73.41251642868386 2 7.323110763132419 12.076316887804275 3 1.8616835483795082 4.605067682758804 4 0.7360414045535726 2.4275665168640463 5 0.34540108138894837 1.4239757690279087 6 0.20841499634358598 1.0310722948586661 7 0.12922714680713 0.745865806266681 8 0.08203434498052084 0.5411212681122801 9 0.05909263341581946 0.43851530514276715 >10 0.212493382918401 2.734162549788175 >50 0.005773455636972068 0.3121983724179018 >100 0.001582139772609016 0.21071882311711915 >500 4.294675843478334E-5 0.025816967348728178 >1k 1.0711689706506784E-5 0.015085327808780937 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.140667244510583E-6 2 0.0 2.898467071157408E-5 0.0 0.0 4.140667244510583E-6 3 0.0 2.898467071157408E-5 0.0 0.0 4.140667244510583E-6 4 0.0 6.211000866765874E-5 0.0 4.140667244510583E-6 4.140667244510583E-6 5 0.0 7.453201040119049E-5 0.0 8.281334489021166E-6 4.140667244510583E-6 6 0.0 7.453201040119049E-5 0.0 8.281334489021166E-6 1.2422001733531749E-5 7 0.0 7.867267764570108E-5 0.0 8.281334489021166E-6 1.2422001733531749E-5 8 0.0 8.695401213472224E-5 0.0 8.281334489021166E-6 1.2422001733531749E-5 9 0.0 1.9047069324748683E-4 0.0 1.6562668978042333E-5 2.4844003467063497E-5 10-11 0.0 1.9047069324748683E-4 0.0 2.2773669844808205E-5 2.4844003467063497E-5 12-13 0.0 1.9254102686974212E-4 0.0 2.691433708931879E-5 2.4844003467063497E-5 14-15 0.0 2.7742470538220904E-4 0.0 3.5195671578339954E-5 4.140667244510583E-5 16-17 0.0 2.960577079825067E-4 0.0 4.140667244510583E-5 5.796934142314816E-5 18-19 0.0 3.2504237869408076E-4 0.0 6.211000866765874E-5 6.211000866765874E-5 20-21 0.0 3.6644905113918656E-4 0.0 9.937601386825399E-5 6.211000866765874E-5 22-23 4.140667244510583E-6 4.4512172878488767E-4 0.0 4.078557235842924E-4 7.24616767789352E-5 24-25 4.140667244510583E-6 4.968800693412699E-4 0.0 0.0014368115338451721 8.281334489021165E-5 26-27 4.140667244510583E-6 5.134427383193123E-4 0.0 0.004113752907421264 8.281334489021165E-5 28-29 4.140667244510583E-6 5.134427383193123E-4 0.0 0.014575148700677252 8.281334489021165E-5 30-31 4.140667244510583E-6 5.610604116311839E-4 0.0 0.017229316404408536 8.281334489021165E-5 32-33 4.140667244510583E-6 5.631307452534393E-4 0.0 0.02343203593668539 8.695401213472224E-5 34-35 6.211000866765874E-6 6.480144237659062E-4 0.0 0.03142559405221307 8.695401213472224E-5 36-37 8.281334489021166E-6 7.370387695228838E-4 0.0 0.045930351409733644 8.695401213472224E-5 38-39 8.281334489021166E-6 7.763751083457342E-4 0.0 0.08297897157999208 8.695401213472224E-5 40-41 8.281334489021166E-6 8.364147833911377E-4 0.0 0.11910008228748015 8.695401213472224E-5 42-43 1.0351668111276457E-5 8.633291204804565E-4 0.0 0.14147210740957084 9.316501300148812E-5 44-45 1.2422001733531749E-5 9.420017981261576E-4 0.0 0.17513159144019735 9.937601386825399E-5 46-47 1.2422001733531749E-5 9.979008059270505E-4 0.0 0.21140590683573232 1.0351668111276457E-4 48-49 1.2422001733531749E-5 0.0010061821404160716 0.0 0.24194125743037562 1.0765734835727516E-4 50-51 1.2422001733531749E-5 0.0011117691551510915 0.0 0.2710087414868399 1.0765734835727516E-4 52-53 1.2422001733531749E-5 0.0011200504896401128 0.0 0.3481887085908949 1.1386834922404103E-4 54-55 1.2422001733531749E-5 0.0011345428249958998 0.0 0.4420679866920611 1.2629035095757278E-4 56-57 1.2422001733531749E-5 0.0011552461612184526 0.0 0.5334980601180993 1.3043101820208337E-4 58-59 1.2422001733531749E-5 0.0011593868284629633 0.0 0.580459437671716 1.3250135182433866E-4 60-61 1.2422001733531749E-5 0.001186301165552282 0.0 0.608007296849445 1.3250135182433866E-4 62-63 1.2422001733531749E-5 0.001215285836263856 0.0 0.6270854211785275 1.3457168544659396E-4 64-65 1.2422001733531749E-5 0.0013664201906884923 0.0 0.6343688548616215 1.3664201906884923E-4 66-67 1.2422001733531749E-5 0.0013809125260442795 0.0 0.6410042741209498 1.3871235269110452E-4 68-69 1.2422001733531749E-5 0.00142024886486713 0.0 0.6477763353993469 1.4078268631335982E-4 70-71 1.2422001733531749E-5 0.0014471632019564488 0.0 0.6531695544853219 1.449233535578704E-4 72-73 1.2422001733531749E-5 0.001449233535578704 0.0 0.6575027627567023 1.5320468804689156E-4 74-75 1.2422001733531749E-5 0.001498921542512831 0.0 0.6609726419076021 1.5734535529140215E-4 76-77 1.2422001733531749E-5 0.0015631018848027449 0.0 0.6641216193470524 1.6148602253591274E-4 78-79 1.2422001733531749E-5 0.0015900162218920638 0.0 0.6662644146460865 1.6148602253591274E-4 80-81 1.2422001733531749E-5 0.0015941568891365745 0.0 0.6670449304216768 1.8425969238072094E-4 82-83 1.2422001733531749E-5 0.0016024382236255956 0.0 0.6675956391651967 2.1117402947003972E-4 84-85 1.2422001733531749E-5 0.0016065788908701062 0.0 0.6678482198671118 2.1117402947003972E-4 86-87 1.2422001733531749E-5 0.0016252118934704037 0.0 0.667962088216336 2.153146967145503E-4 88 1.2422001733531749E-5 0.0017059549047383602 0.0 0.6679641585499582 2.1945536395906088E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 31425 0.0 51.72673 1 GTATCAA 55445 0.0 34.78978 1 TCAACGC 70350 0.0 26.903276 4 ATCAACG 71465 0.0 26.489286 3 CAACGCA 71780 0.0 26.374023 5 AACGCAG 73015 0.0 25.944986 6 TATCAAC 73620 0.0 25.879229 2 ACGCAGA 84650 0.0 22.359455 7 GTACATG 72890 0.0 21.114862 1 CGCAGAG 91355 0.0 20.713837 8 TACATGG 73940 0.0 20.58455 2 GTGGTAT 13860 0.0 20.511402 1 ACATGGG 73715 0.0 20.042143 3 TGGTATC 13690 0.0 19.005241 2 GCAGAGT 98965 0.0 18.797977 9 GAGTACT 59140 0.0 18.454252 12-13 AGAGTAC 90605 0.0 18.239628 10-11 CAGAGTA 97120 0.0 17.851917 10-11 TATATAC 31305 0.0 17.376074 3 CATGGGG 54785 0.0 17.356766 4 >>END_MODULE