FastQCFastQC Report
Fri 27 May 2016
SRR2139194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20640699
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA269400.051.6098371
GTATCAA487050.035.2313581
TCAACGC613550.027.4517384
ATCAACG620550.027.1489263
CAACGCA629100.026.7934655
TATCAAC636450.026.5742242
AACGCAG639150.026.3983276
ACGCAGA744100.022.6529437
CGCAGAG794100.021.2424228
GTACATG660250.020.7800561
GACCGAG191800.020.2876290-91
TACATGG660800.020.257432
ACCGAGG199000.020.19752990-91
CGAGACC196550.019.95092286-87
GTGGTAT130050.019.8992541
AGACCGA199250.019.79655588-89
ACATGGG667000.019.637683
GAGACCG197150.019.48695688-89
TGGTATC121250.019.3124122
GCAGAGT870650.019.1496529
Produced by FastQC (version 0.11.5)