##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139194_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20640699
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.359097770865223	31.0	31.0	34.0	30.0	34.0
2	31.47255114761375	31.0	31.0	34.0	30.0	34.0
3	31.548043213071416	31.0	31.0	34.0	30.0	34.0
4	35.368024115849956	37.0	35.0	37.0	33.0	37.0
5	35.16228151963264	37.0	35.0	37.0	32.0	37.0
6	35.180592430517976	37.0	35.0	37.0	32.0	37.0
7	35.08609039839203	37.0	35.0	37.0	32.0	37.0
8	35.015555432497706	37.0	35.0	37.0	32.0	37.0
9	36.546263815968636	39.0	35.0	39.0	32.0	39.0
10-11	36.463418801853564	38.0	35.0	39.0	32.0	39.0
12-13	36.42768304503641	38.0	35.0	39.0	32.0	39.0
14-15	37.49146026982904	39.0	36.0	41.0	32.0	41.0
16-17	37.45040383564529	39.0	36.0	41.0	32.0	41.0
18-19	37.474764856558394	39.0	36.0	41.0	32.0	41.0
20-21	37.481980207155964	39.0	36.0	41.0	32.0	41.0
22-23	37.38149712387269	39.0	36.0	41.0	31.5	41.0
24-25	37.2109675981703	39.0	36.0	41.0	31.0	41.0
26-27	37.059113918430725	39.0	36.0	41.0	31.0	41.0
28-29	36.8893013160251	39.0	36.0	40.0	30.0	41.0
30-31	36.74699050218034	39.0	35.0	40.0	30.0	41.0
32-33	36.57911776108499	38.0	35.0	40.0	30.0	41.0
34-35	36.42162688495053	38.0	35.0	40.0	30.0	41.0
36-37	36.26776271444652	38.0	35.0	40.0	30.0	41.0
38-39	36.134919774349775	38.0	35.0	40.0	29.5	41.0
40-41	36.006334727377904	38.0	34.0	40.0	29.0	41.0
42-43	35.845073666095715	38.0	34.0	40.0	29.0	41.0
44-45	35.685288254500094	38.0	34.0	40.0	28.0	41.0
46-47	35.84179480091812	38.0	34.0	40.0	28.5	41.0
48-49	35.979222022047296	38.0	34.0	40.0	28.5	41.0
50-51	36.193057732685375	38.0	34.0	40.0	29.0	41.0
52-53	36.16566823279058	38.0	34.0	40.0	29.0	41.0
54-55	36.06172208737277	38.0	34.0	40.0	28.5	41.0
56-57	35.90427175780931	38.0	34.0	40.0	28.0	41.0
58-59	35.700007260300104	38.0	34.0	40.0	28.0	41.0
60-61	35.524399475355864	37.5	34.0	40.0	28.0	41.0
62-63	35.312629056043356	37.0	33.0	40.0	28.0	41.0
64-65	35.09932452446691	37.0	33.0	39.5	28.0	41.0
66-67	34.84603507724699	36.0	33.0	39.0	28.0	41.0
68-69	34.57288621189654	36.0	32.5	39.0	27.0	40.5
70-71	34.262350359661454	35.0	32.0	39.0	26.5	40.0
72-73	34.003408002820635	35.0	32.0	38.0	26.0	40.0
74-75	33.64554719188121	35.0	32.0	37.0	26.0	39.5
76-77	31.973745207650722	33.5	29.5	35.5	25.0	38.5
78-79	33.01914041589752	34.5	31.0	36.0	26.0	39.0
80-81	33.118236328194875	35.0	32.0	36.0	26.0	38.5
82-83	32.94578275401951	34.5	32.0	36.0	26.0	37.0
84-85	32.76471112151313	34.0	31.0	35.0	26.0	37.0
86-87	32.582130256603165	34.0	31.0	35.0	26.0	36.5
88-89	32.42262286095006	34.0	31.0	35.0	26.0	36.0
90-91	32.328818909417926	34.0	31.0	35.0	26.0	36.0
92-93	32.27863143325652	34.0	31.0	35.0	26.0	36.0
94-95	32.30147633348573	34.0	31.0	35.0	26.5	35.0
96-97	32.40018758747164	34.0	31.0	35.0	27.0	35.0
98-99	32.554070392938044	34.0	31.0	35.0	27.5	35.0
100	31.495497318391813	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	10.0
19	57.0
20	383.0
21	2272.0
22	10289.0
23	33852.0
24	82979.0
25	164076.0
26	273658.0
27	403341.0
28	541460.0
29	677101.0
30	810911.0
31	955602.0
32	1122319.0
33	1331412.0
34	1623596.0
35	2021463.0
36	2632373.0
37	3397632.0
38	3207072.0
39	1311216.0
40	37623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.312849870055274	21.24006071693599	14.78411172024746	23.662977692761277
2	15.07771562889828	22.247328787445692	40.25818172965202	22.416773854004006
3	18.123216779835584	26.85656804216567	31.15915905673452	23.86105612126423
4	12.812255912861795	18.955730241859257	38.47381593302004	29.758197912258904
5	12.742228351859595	38.398806164461774	36.020461322555015	12.838504161123613
6	29.63260595002136	38.872990686991756	18.511810089377303	12.98259327360958
7	25.919059233410653	33.03829972037284	23.56895471417901	17.473686332037495
8	24.541814208908335	37.004996778452124	20.923584031722957	17.529604980916584
9	25.55079157127029	16.105553560131415	21.110290491924296	37.233364376674
10-11	24.12143883662401	26.8750883276412	29.141525335577207	19.86194750015758
12-13	24.90930761000689	24.608082061524293	29.07202623616898	21.410584092299835
14-15	22.015457298250073	25.82241938313002	26.761056526724747	25.401066791895154
16-17	21.559504762197296	28.926503913071063	27.327650060285226	22.186341264446416
18-19	21.652207376018705	28.040153691320786	29.018965201242036	21.288673731418474
20-21	22.553832887750637	27.128394946102123	28.945188665027434	21.372583501119806
22-23	22.270493292457555	27.111063554838786	28.75036654594578	21.868076606757878
24-25	22.066701621638472	27.269988217627084	28.868221682236584	21.795088478497863
26-27	21.98637414517005	27.305761029973684	28.80933658765451	21.898528237201756
28-29	22.03201212465861	27.35544285068926	28.75682240253447	21.855722622117653
30-31	22.34425471141395	27.13566380184997	28.672568949697876	21.84751253703821
32-33	22.189281657530223	27.281090352495035	28.565232717080008	21.96439527289473
34-35	22.232907074054857	27.391175962650884	28.59186256844024	21.784054394854017
36-37	22.35064781535905	27.30286322035872	28.395869690250862	21.950619274031364
38-39	22.051885429826694	27.519590977257273	28.493314674658848	21.935208918257185
40-41	22.274627787996632	27.301201640713195	28.432304057539554	21.99186651375062
42-43	22.12795135702396	27.4995704082266	28.120764759513634	22.251713475235807
44-45	22.143278196178155	27.56033620189357	27.981602893299613	22.314782708628663
46-47	22.301771374028323	27.518873806914428	27.7732818062691	22.406073012788145
48-49	22.29874850357576	27.769536435024516	27.595563883837098	22.33615117756262
50-51	22.298627804900846	27.77014035626654	27.534771208631014	22.39646063020159
52-53	22.431576120128764	27.682108645306432	27.48373731895027	22.40257791561454
54-55	22.439086704583648	27.576650289622574	27.526970732855794	22.457292272937988
56-57	22.462221912603592	27.739809602405025	27.34986428879559	22.4481041961958
58-59	22.311898152067837	27.66657092868141	27.467041624688466	22.554489294562284
60-61	22.523389650085797	27.65670946387565	27.535807155561145	22.284093730477412
62-63	22.297982378891245	27.70141617768307	27.565699161191493	22.434902282234194
64-65	22.28505820570009	27.647217918540175	27.508537537065358	22.55918633869438
66-67	22.385676002206733	27.759347687753817	27.411830020325944	22.44314628971351
68-69	22.326988595871182	27.82996486121735	27.367786694293216	22.475259848618254
70-71	22.297438848156904	27.642032600465395	27.244704328876413	22.815824222501295
72-73	22.331094624907966	27.65223298146059	27.129850797127453	22.886821596503992
74-75	22.30502296814057	27.728000911101542	27.33042986802997	22.636546252727918
76-77	22.385381155209696	27.486193356891718	27.31687911718791	22.811546370710673
78-79	22.280886614072536	27.509095929103033	27.095324438321562	23.114693018502873
80-81	22.55874221891105	27.677648956672186	27.139172972606872	22.624435851809892
82-83	22.653176094344392	27.388755802505887	27.332297721716937	22.62577038143278
84-85	22.454547847450623	27.401061948249765	27.24213124127689	22.902258963022724
86-87	22.29952588440348	27.723372997773453	27.25706057995531	22.72004053786776
88-89	22.656043244700705	27.559080623894953	27.23591778796206	22.54895834344228
90-91	22.710945663641063	27.545341827506288	27.26293653684031	22.48077597201234
92-93	22.51117834506718	27.273391109895744	27.40984942852182	22.805581116515256
94-95	22.413080971912237	27.626369131834334	27.54980117477383	22.4107487214796
96-97	22.633968447502234	27.45756713714012	27.682345576798006	22.22611883855964
98-99	23.41890133207668	29.62895594278644	27.930143085691206	19.021999639445678
100	22.59966895860254	30.432428227545582	21.663919512859188	25.303983300992694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5829.0
1	4926.5
2	6213.0
3	8817.5
4	11244.0
5	17986.0
6	24646.0
7	30311.5
8	34774.0
9	35142.0
10	32006.0
11	27921.0
12	29044.0
13	37355.5
14	59113.0
15	90857.5
16	116248.5
17	130017.0
18	134754.0
19	132332.0
20	124352.0
21	113949.0
22	106966.0
23	109546.0
24	123775.0
25	150396.5
26	187361.5
27	227518.0
28	270949.5
29	320999.5
30	369989.5
31	417987.5
32	466524.0
33	510509.0
34	552535.0
35	592201.5
36	634155.5
37	668713.0
38	689146.0
39	708137.5
40	729216.0
41	744694.0
42	751402.0
43	759974.5
44	771870.5
45	791979.0
46	814008.0
47	836308.0
48	852051.0
49	851044.0
50	843321.0
51	827916.5
52	791311.0
53	740872.5
54	698603.5
55	660169.0
56	619419.5
57	570132.5
58	513727.0
59	452190.5
60	384887.5
61	323774.0
62	264873.5
63	213159.5
64	172681.0
65	136961.5
66	109068.5
67	82381.0
68	57952.0
69	42644.0
70	31524.5
71	21979.5
72	15545.5
73	11180.5
74	8239.5
75	5976.5
76	4063.0
77	2531.0
78	1765.5
79	1324.5
80	959.5
81	694.0
82	554.5
83	381.5
84	233.0
85	160.0
86	120.5
87	97.0
88	70.5
89	51.0
90	37.5
91	29.5
92	22.0
93	12.5
94	4.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.10191999796130935
3	9.689594330114499E-5
4	8.236155180597324E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.008778772463083735
10-11	0.0010561657819824805
12-13	8.987098741181197E-4
14-15	1.380767192041316E-4
16-17	3.367134029714788E-4
18-19	5.135484994960684E-4
20-21	1.2355186913386022E-4
22-23	0.0
24-25	0.14897160963174566
26-27	0.0014699389123951096
28-29	0.001527017886768929
30-31	0.0022225106676864604
32-33	0.0019334676471615135
34-35	0.00140936595121607
36-37	2.483345053000853E-4
38-39	3.3155764420375995E-4
40-41	7.551209160419183E-4
42-43	8.41467815262699E-4
44-45	9.12891164354643E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.5934826559372168E-4
56-57	0.0
58-59	7.02168087103195E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0035411313661166317
70-71	0.005736263236542837
72-73	5.844835287281253E-6
74-75	2.456511242016524E-4
76-77	0.0014540276626214517
78-79	0.009180248161779772
80-81	7.080708968607615E-5
82-83	6.561618897612405E-5
84-85	1.585657537296251E-4
86-87	3.8661481517637125E-5
88-89	3.606434048829084E-5
90-91	0.0
92-93	2.0511818414067907E-5
94-95	2.1042856618288787E-5
96-97	7.262278834106494E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	960.0
20-21	3187.0
22-23	6549.0
24-25	13122.0
26-27	15360.0
28-29	26884.0
30-31	52336.0
32-33	66500.0
34-35	92405.0
36-37	122160.0
38-39	140733.0
40-41	152930.0
42-43	179018.0
44-45	216679.0
46-47	200192.0
48-49	182477.0
50-51	155797.0
52-53	147017.0
54-55	160976.0
56-57	179709.0
58-59	120727.0
60-61	193778.0
62-63	189348.0
64-65	200305.0
66-67	215661.0
68-69	221719.0
70-71	221431.0
72-73	214670.0
74-75	221009.0
76-77	201520.0
78-79	224952.0
80-81	239337.0
82-83	235477.0
84-85	242230.0
86-87	262949.0
88-89	288736.0
90-91	321263.0
92-93	354884.0
94-95	427333.0
96-97	969393.0
98-99	1389436.0
100-101	1.156955E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.68563081070984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.91988463804469	73.52396752912273
2	7.390739122559823	12.222158530125023
3	1.9200829397857222	4.762898072551939
4	0.7664884797039233	2.5351033381384345
5	0.3635676366389904	1.5030909688926932
6	0.1930411813675333	0.9577039112291482
7	0.1257999322024326	0.7281292725071868
8	0.07161316010281892	0.4737103453959953
9	0.054388300862403455	0.40474178689774537
>10	0.1885641693762786	2.401968248213611
>50	0.0044853923439467785	0.24821193714868794
>100	0.0012869779155073988	0.1756480142716812
>500	2.3229072277086088E-5	0.018208288857137073
>1k	3.4840023600450165E-5	0.044459756647987646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.3603174485515245E-5	0.0	0.0	0.0
3	0.0	4.3603174485515245E-5	0.0	0.0	0.0
4	0.0	9.689594330114499E-5	0.0	4.84479716505725E-6	0.0
5	0.0	1.0658553763125948E-4	0.0	4.84479716505725E-6	0.0
6	4.84479716505725E-6	1.0658553763125948E-4	0.0	4.84479716505725E-6	4.84479716505725E-6
7	4.84479716505725E-6	1.1143033479631674E-4	0.0	4.84479716505725E-6	4.84479716505725E-6
8	4.84479716505725E-6	1.2111992912643123E-4	0.0	4.84479716505725E-6	4.84479716505725E-6
9	4.84479716505725E-6	1.9379188660228997E-4	0.0	2.4223985825286247E-5	9.6895943301145E-6
10-11	1.2111992912643123E-5	1.986366837673472E-4	0.0	3.633597873792937E-5	9.6895943301145E-6
12-13	1.9379188660229E-5	1.986366837673472E-4	0.0	3.8758377320458E-5	9.6895943301145E-6
14-15	1.9379188660229E-5	3.052222213986067E-4	0.0	3.8758377320458E-5	2.664638440781487E-5
16-17	2.4223985825286247E-5	3.2460141005883573E-4	0.0	5.0870370233101114E-5	3.391358015540074E-5
18-19	2.9068782990343494E-5	3.5609259163170784E-4	0.0	6.298236314574424E-5	4.602557306804387E-5
20-21	2.9068782990343494E-5	3.9242857036963716E-4	0.0	1.1627513196137398E-4	4.844797165057249E-5
22-23	3.391358015540074E-5	4.311869476900952E-4	0.0	3.730493817094082E-4	6.055996456321561E-5
24-25	5.813756598068699E-5	4.675229264280245E-4	0.0	0.00135654320621603	7.267195747585874E-5
26-27	6.540476172827286E-5	4.8447971650572493E-4	0.0	0.003728071418511553	7.7516754640916E-5
28-29	7.267195747585874E-5	4.893245136707822E-4	0.0	0.013492760104684438	7.7516754640916E-5
30-31	7.267195747585874E-5	5.644188697291696E-4	0.0	0.016351190432068216	7.993915322344461E-5
32-33	7.267195747585874E-5	5.668412683116982E-4	0.0	0.02274147789277873	8.236155180597324E-5
34-35	7.509435605838736E-5	6.298236314574424E-4	0.0	0.03033085265184091	8.720634897103049E-5
36-37	9.931834188367362E-5	6.928059946031866E-4	0.0	0.0435401921223695	8.962874755355911E-5
38-39	1.0174074046620224E-4	7.29141973341116E-4	0.0	0.07884665146272421	9.931834188367362E-5
40-41	1.0174074046620224E-4	7.897019379043316E-4	0.0	0.11624121838121858	1.0416313904873086E-4
42-43	1.1143033479631674E-4	8.018139308169747E-4	0.0	0.1385151733475693	1.090079362137881E-4
44-45	1.1143033479631674E-4	9.083994684482343E-4	0.0	0.16920938578678948	1.1143033479631674E-4
46-47	1.1143033479631674E-4	0.001000450614584322	0.0	0.2038206167339585	1.1385273337884536E-4
48-49	1.1627513196137398E-4	0.0010052954117493792	0.0	0.23426532211917822	1.3080952345654573E-4
50-51	1.2354232770895985E-4	0.0011070361522155815	0.0	0.26395666154523156	1.3080952345654573E-4
52-53	1.3080952345654573E-4	0.0011215705437107532	0.0	0.33774292236905346	1.5261111069930335E-4
54-55	1.4049911778666024E-4	0.0011288377394583391	0.0	0.42899709937148933	1.7925749510711822E-4
56-57	1.5261111069930335E-4	0.001148216928118568	0.0	0.5185555973661551	1.865246908547041E-4
58-59	1.5987830644688923E-4	0.0011579065224486826	0.0	0.7349242387576118	1.9136948801976137E-4
60-61	1.6472310361194647E-4	0.0011966648997691405	0.0	0.9177135909980567	1.9379188660228997E-4
62-63	1.695679007770037E-4	0.001225733682759484	0.0	0.9268799472343452	1.986366837673472E-4
64-65	1.7199029935953235E-4	0.001400146380701545	0.0	0.9344596323990773	2.0105908234987585E-4
66-67	1.7925749510711822E-4	0.0014171031707792454	0.0	0.9414773210926626	2.0832627809746172E-4
68-69	1.8410229227217546E-4	0.0014534391495171746	0.0	0.9484223378287722	2.1317107526251896E-4
70-71	1.8410229227217546E-4	0.0014800855339249896	0.0	0.9537152787315972	2.180158724275762E-4
72-73	1.8410229227217546E-4	0.0014994647225852185	0.0	0.9578309339233133	2.4223985825286247E-4
74-75	1.8410229227217546E-4	0.001543067897070734	0.0	0.9612004903516107	2.4223985825286247E-4
76-77	1.8410229227217546E-4	0.001613317455964064	0.0	0.9642066869925288	2.4223985825286247E-4
78-79	1.865246908547041E-4	0.001652075833284522	0.0	0.9662463465990179	2.4223985825286247E-4
80-81	1.8894708943723273E-4	0.0016544982318670506	0.0	0.9668810150276403	2.470846554179197E-4
82-83	1.8894708943723273E-4	0.0016617654276146366	0.0	0.9674696578831947	2.567742497480342E-4
84-85	1.8894708943723273E-4	0.001671455021944751	0.0	0.9677361217272729	2.567742497480342E-4
86-87	1.9379188660228997E-4	0.001710213399265209	0.0	0.9678863104393897	2.591966483305628E-4
88	1.9379188660228997E-4	0.0017925749510711822	0.0	0.9678984224323023	2.664638440781487E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	26940	0.0	51.609837	1
GTATCAA	48705	0.0	35.231358	1
TCAACGC	61355	0.0	27.451738	4
ATCAACG	62055	0.0	27.148926	3
CAACGCA	62910	0.0	26.793465	5
TATCAAC	63645	0.0	26.574224	2
AACGCAG	63915	0.0	26.398327	6
ACGCAGA	74410	0.0	22.652943	7
CGCAGAG	79410	0.0	21.242422	8
GTACATG	66025	0.0	20.780056	1
GACCGAG	19180	0.0	20.28762	90-91
TACATGG	66080	0.0	20.25743	2
ACCGAGG	19900	0.0	20.197529	90-91
CGAGACC	19655	0.0	19.950922	86-87
GTGGTAT	13005	0.0	19.899254	1
AGACCGA	19925	0.0	19.796555	88-89
ACATGGG	66700	0.0	19.63768	3
GAGACCG	19715	0.0	19.486956	88-89
TGGTATC	12125	0.0	19.312412	2
GCAGAGT	87065	0.0	19.149652	9
>>END_MODULE