FastQCFastQC Report
Fri 27 May 2016
SRR2139193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19266144
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA238550.047.558131
ATCTCGT155450.045.3797294
TATCTCG194800.034.07613494
GTATCAA411800.033.9437751
CCGTCTT87950.030.70728990-91
TATGCCG85800.030.39415486-87
GCCGTCT94600.029.68414790-91
ATGCCGT93550.029.27620988-89
TGCCGTC94850.027.8738788-89
TCAACGC507350.026.7989354
TCGTATG101750.026.6491684-85
CTCGTAT96150.026.61695782-83
ATCAACG511600.026.5845763
CAACGCA517800.026.2421655
TCTCGTA101550.025.80357682-83
AACGCAG529900.025.6750626
TATCAAC539750.025.4502142
CGCTATC299500.024.89455294
CTATCTC307000.024.47667594
CGTATGC110450.023.83175584-85
Produced by FastQC (version 0.11.5)