##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19266144
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.484946909978458	31.0	31.0	34.0	30.0	34.0
2	31.598135205467166	31.0	31.0	34.0	30.0	34.0
3	31.681790139220386	31.0	31.0	34.0	30.0	34.0
4	35.47845801422433	37.0	35.0	37.0	33.0	37.0
5	35.27733037809745	37.0	35.0	37.0	33.0	37.0
6	35.28677087641409	37.0	35.0	37.0	32.0	37.0
7	35.196453374375274	37.0	35.0	37.0	32.0	37.0
8	35.121683612455094	37.0	35.0	37.0	32.0	37.0
9	36.6653536379672	39.0	35.0	39.0	32.0	39.0
10-11	36.59981405723948	39.0	35.0	39.0	32.0	39.0
12-13	36.56528864831489	39.0	35.0	39.0	32.0	39.0
14-15	37.67311912544618	39.0	36.0	41.0	32.0	41.0
16-17	37.63700369933911	39.0	36.0	41.0	32.0	41.0
18-19	37.65548303282691	39.5	36.5	41.0	32.0	41.0
20-21	37.66092962268459	39.5	36.5	41.0	32.0	41.0
22-23	37.56441507946833	39.0	36.0	41.0	32.0	41.0
24-25	37.38538254976869	39.0	36.0	41.0	31.5	41.0
26-27	37.26075077982621	39.0	36.0	41.0	31.0	41.0
28-29	37.1028207312913	39.0	36.0	41.0	31.0	41.0
30-31	36.971381103115235	39.0	36.0	40.0	30.5	41.0
32-33	36.81803621345752	39.0	35.0	40.0	30.0	41.0
34-35	36.67244328651595	38.5	35.0	40.0	30.0	41.0
36-37	36.52720517858651	38.0	35.0	40.0	30.0	41.0
38-39	36.41390453627666	38.0	35.0	40.0	30.0	41.0
40-41	36.289613350278046	38.0	35.0	40.0	30.0	41.0
42-43	36.13157574196932	38.0	35.0	40.0	29.5	41.0
44-45	35.981819472126034	38.0	34.0	40.0	29.0	41.0
46-47	36.14104076770603	38.0	35.0	40.0	29.0	41.0
48-49	36.28055981377574	38.0	35.0	40.0	29.5	41.0
50-51	36.48651234814017	38.5	35.0	40.0	30.0	41.0
52-53	36.46272651692495	38.0	35.0	40.0	30.0	41.0
54-55	36.3641587083763	38.0	35.0	40.0	29.5	41.0
56-57	36.212733403798666	38.0	34.0	40.0	29.0	41.0
58-59	36.029542665090744	38.0	34.0	40.0	28.5	41.0
60-61	35.853054341038344	38.0	34.0	40.0	28.0	41.0
62-63	35.654546892741415	37.0	34.0	40.0	28.0	41.0
64-65	35.44355215182924	37.0	34.0	40.0	28.0	41.0
66-67	35.19713979392158	36.5	33.0	39.5	28.0	41.0
68-69	34.91940914971753	36.0	33.0	39.0	28.0	41.0
70-71	34.60345174139987	36.0	33.0	39.0	28.0	40.5
72-73	34.3433205708059	35.0	32.5	38.5	28.0	40.0
74-75	33.96170421116648	35.0	32.0	37.5	27.0	40.0
76-77	32.25003903090821	33.5	30.0	36.0	25.5	38.5
78-79	33.24478995188742	34.5	31.5	36.0	26.0	39.0
80-81	33.308602750735176	35.0	32.0	36.0	27.0	39.0
82-83	33.12872246592136	35.0	32.0	36.0	27.0	37.0
84-85	32.94850813669103	34.5	32.0	35.0	27.0	37.0
86-87	32.7603059747442	34.0	32.0	35.0	27.0	36.5
88-89	32.60300685331696	34.0	32.0	35.0	26.0	36.0
90-91	32.5224526404136	34.0	32.0	35.0	27.0	36.0
92-93	32.483965121797254	34.0	32.0	35.0	27.0	36.0
94-95	32.480558338027734	34.0	32.0	35.0	27.0	35.0
96-97	32.55097625430996	34.0	32.0	35.0	27.0	35.0
98-99	32.68382154158026	34.0	32.0	35.0	28.0	35.0
100	31.688242572881563	34.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	37.0
20	276.0
21	1729.0
22	8202.0
23	27677.0
24	68661.0
25	136712.0
26	231167.0
27	343575.0
28	462896.0
29	583238.0
30	703278.0
31	833350.0
32	982971.0
33	1177120.0
34	1442842.0
35	1813213.0
36	2413513.0
37	3274620.0
38	3312268.0
39	1398425.0
40	50366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38252283383743	21.209729357363884	14.726423720283623	23.681324088515066
2	14.704065215884409	21.95411226735503	40.91487804393916	22.426944472821397
3	17.830653543460347	27.083017472377037	31.239234667064604	23.847094317098016
4	12.693899225942065	18.604318490756498	38.995295837581075	29.706486445720365
5	12.702941491561571	38.5162282603099	36.07389210835339	12.706938139775142
6	29.562282935287932	38.874566701048224	18.662125643823693	12.901024719840152
7	25.737153215505913	33.154117398894144	23.91983055872519	17.18889882687475
8	24.23520762639374	37.23563469680285	21.21991302463015	17.309244652173263
9	25.490435170354754	16.40305408022658	21.35160984973836	36.75490089968031
10-11	24.061072237382252	26.798268176806744	29.46781623257501	19.67284335323599
12-13	24.758562106847464	24.515226438396244	29.483157085265248	21.24305436949105
14-15	21.974513341931676	25.874214057480543	26.825137257142	25.32613534344578
16-17	21.55458641396411	28.93195791400387	27.52525475348483	21.98820091854719
18-19	21.614679282892464	28.21830790157592	29.11636495653893	21.05064785899269
20-21	22.430185752431242	27.34912322346347	28.967308649706357	21.253382374398928
22-23	22.22248209018011	27.264495297690743	28.78826611201096	21.724756500118186
24-25	21.988805544245977	27.452543375810766	28.77405788824252	21.784593191700736
26-27	21.890506301949213	27.558366075174444	28.72441192251618	21.826715700360168
28-29	21.984862915625282	27.557157836102782	28.726652537930853	21.731326710341087
30-31	22.162943930026564	27.464180899901503	28.581250566515852	21.791624603556077
32-33	21.968604967257978	27.65367634330225	28.541236227547166	21.836482461892604
34-35	22.094585610559644	27.63235154978171	28.54872460133674	21.724338238321902
36-37	22.117698799066606	27.576438871639663	28.455880897824237	21.84998143146949
38-39	21.982512737007305	27.659759826975005	28.45209812271414	21.90562931330355
40-41	22.19569951770976	27.54263684365451	28.430649977846755	21.831013660788976
42-43	22.014410763415825	27.624754638530476	28.237976731861075	22.12285786619263
44-45	22.059078631348715	27.699654099586095	28.065388848129125	22.17587842093606
46-47	22.127734235142686	27.664390099032783	28.00674526822297	22.201130397601563
48-49	22.085413802505695	27.906843035742963	27.79579153643742	22.21195162531392
50-51	22.06088761171593	28.041972857966535	27.55327504675387	22.34386448356366
52-53	22.042113770411486	27.80427586466811	27.54475922874473	22.608851136175677
54-55	22.20388878012644	27.545544077084706	27.849608078969762	22.400959063819094
56-57	22.003235740695633	27.259366311690066	28.00116771446102	22.73623023315328
58-59	21.879920451984816	27.051839186897443	28.024369234115802	23.04387112700194
60-61	22.04994145657757	27.296546949252942	28.311651769708547	22.341859824460936
62-63	21.639590260147433	27.581815346064232	28.26396613309381	22.51462826069453
64-65	21.481457955513765	27.833712212294166	27.896477372970114	22.788352459221954
66-67	21.537820941183092	28.40120552189363	27.452809870165158	22.60816366675812
68-69	21.58177420507508	28.397261493944193	27.274154267342183	22.746810033638546
70-71	21.611852738036543	27.88422110323628	27.234610614240484	23.26931554448669
72-73	21.900333396515055	27.40124827554313	27.178613228259234	23.519805099682586
74-75	22.019728757363566	27.47111536246811	27.387441778824183	23.121714101344143
76-77	22.317174031582912	27.304178771470962	26.86615122200599	23.51249597494013
78-79	22.475140925816014	27.357403992456664	26.41805333866304	23.749401743064286
80-81	22.38524839185177	27.6902097189893	26.538140393189508	23.386401495969423
82-83	22.32518091495115	27.447409302842747	26.627860565882266	23.599549216323837
84-85	22.370601069182037	27.864445536243736	26.60673170520112	23.15822168937311
86-87	22.83619867126938	27.70971320822997	26.735481096643042	22.71860702385761
88-89	22.555455465843245	27.850283120159986	27.06866911589672	22.52559229810005
90-91	22.535391649031713	27.4686327379884	26.97730969205961	23.018665920920274
92-93	22.52976778790455	26.933293585374628	27.24600311600682	23.290935510714
94-95	21.75370189061408	27.313108288228932	27.970469251153666	22.962720570003317
96-97	21.884566760680283	27.229957736443062	28.11544077387072	22.770034729005932
98-99	23.096463788705346	29.107773970098876	28.47807320268277	19.317689038513006
100	22.346954364143702	30.05172795281817	21.996536886267357	25.604780796770772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3763.0
1	3331.5
2	4778.0
3	6987.0
4	9128.5
5	15078.5
6	20814.0
7	25136.0
8	28104.5
9	27895.5
10	25355.0
11	22403.0
12	23696.5
13	30548.5
14	47371.5
15	71349.0
16	90914.0
17	102384.0
18	107148.0
19	106131.0
20	100918.0
21	94672.5
22	91509.0
23	96518.5
24	110940.5
25	133792.0
26	164965.0
27	202833.0
28	248314.5
29	301180.0
30	351631.5
31	401085.5
32	450792.0
33	492951.0
34	529716.0
35	562327.5
36	596085.0
37	629025.5
38	652480.5
39	668464.0
40	685323.0
41	699978.5
42	709666.0
43	724148.0
44	740834.5
45	759342.0
46	770725.0
47	771034.5
48	772668.0
49	768706.5
50	760560.5
51	748229.5
52	727597.0
53	696775.0
54	656155.0
55	610082.5
56	559812.0
57	507408.0
58	453837.5
59	398118.0
60	335310.5
61	272950.5
62	218430.5
63	169884.5
64	128232.5
65	96742.0
66	73775.5
67	56779.5
68	43470.0
69	32289.5
70	23604.0
71	17291.0
72	12513.5
73	8915.0
74	6259.0
75	4396.0
76	3117.0
77	2148.0
78	1503.0
79	1108.0
80	810.0
81	585.5
82	447.0
83	305.5
84	205.0
85	148.0
86	111.5
87	91.0
88	65.0
89	44.0
90	30.0
91	21.5
92	18.0
93	13.0
94	6.0
95	2.5
96	2.0
97	2.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11035939521681143
3	9.861859228291867E-5
4	7.785678338125159E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.01079095017664147
10-11	0.0010640427062104385
12-13	8.538293910810591E-4
14-15	1.2716607952271093E-4
16-17	3.529507513283405E-4
18-19	5.579736142323031E-4
20-21	1.3755536960491943E-4
22-23	2.5957952944409305E-6
24-25	0.1759882627059306
26-27	0.0016861239919466148
28-29	0.0016925700516655057
30-31	0.0023408713905468687
32-33	0.0020362271436439993
34-35	0.0014275845429318284
36-37	3.657902918993371E-4
38-39	4.1787169535228026E-4
40-41	7.581658451873228E-4
42-43	9.139244265975165E-4
44-45	9.462936960915069E-5
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.0978567959315677E-4
56-57	0.0
58-59	9.269619437459381E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.004356451688757322
70-71	0.006905230688249022
72-73	6.077112972679367E-6
74-75	2.523805099089667E-4
76-77	0.001484330860841425
78-79	0.010578050504142495
80-81	7.36848141685005E-5
82-83	5.2033987429824445E-5
84-85	1.8816501477590536E-4
86-87	4.359218518201699E-5
88-89	3.0702356313740007E-5
90-91	3.478294624146092E-6
92-93	3.9094007048649474E-5
94-95	2.1859079758973042E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	712.0
20-21	2451.0
22-23	5236.0
24-25	9560.0
26-27	12124.0
28-29	22816.0
30-31	46641.0
32-33	59723.0
34-35	82577.0
36-37	109008.0
38-39	85318.0
40-41	137659.0
42-43	155257.0
44-45	187425.0
46-47	175764.0
48-49	163149.0
50-51	91898.0
52-53	34160.0
54-55	36930.0
56-57	37898.0
58-59	110201.0
60-61	186230.0
62-63	180882.0
64-65	190712.0
66-67	205509.0
68-69	213710.0
70-71	215467.0
72-73	209202.0
74-75	215452.0
76-77	198351.0
78-79	219931.0
80-81	232402.0
82-83	228625.0
84-85	231430.0
86-87	248518.0
88-89	273757.0
90-91	300812.0
92-93	332232.0
94-95	397114.0
96-97	882594.0
98-99	1382583.0
100-101	1.1154124E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.57075935149388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.77068630266143	70.71750844207193
2	7.838056116790723	12.630362663388997
3	2.2437357085821477	5.423384694735774
4	0.8936568346494856	2.8801043906944583
5	0.45147965499154064	1.818802931720945
6	0.24581099588962757	1.188310715746454
7	0.1578492568652171	0.8902624142089686
8	0.09408013113331604	0.6064086084239515
9	0.06392353755806161	0.46353311653380863
>10	0.23395799582909696	2.890406245666234
>50	0.0053207692408576744	0.28935301688741016
>100	0.0014016405000209882	0.17894408316996785
>500	4.105530840911923E-5	0.022618676750980983
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.114271335250063E-5	0.0	0.0	0.0
3	5.190452225416773E-6	3.114271335250063E-5	0.0	0.0	0.0
4	5.190452225416773E-6	7.785678338125159E-5	0.0	1.0380904450833545E-5	0.0
5	1.0380904450833545E-5	7.785678338125159E-5	0.0	2.076180890166709E-5	1.0380904450833545E-5
6	1.0380904450833545E-5	7.785678338125159E-5	0.0	2.076180890166709E-5	1.0380904450833545E-5
7	1.0380904450833545E-5	7.785678338125159E-5	0.0	2.076180890166709E-5	1.0380904450833545E-5
8	1.0380904450833545E-5	8.823768783208513E-5	0.0	2.5952261127083862E-5	1.5571356676250317E-5
9	1.0380904450833545E-5	1.868562801150038E-4	0.0	7.266633115583482E-5	1.5571356676250317E-5
10-11	1.5571356676250317E-5	1.868562801150038E-4	0.0	7.785678338125159E-5	1.5571356676250317E-5
12-13	3.633316557791741E-5	1.868562801150038E-4	0.0	1.0121381839562707E-4	2.076180890166709E-5
14-15	4.152361780333418E-5	2.413560284818799E-4	0.0	1.0640427062104384E-4	3.114271335250063E-5
16-17	4.9309296141459335E-5	2.4914170682000506E-4	0.0	1.14189948959169E-4	3.114271335250063E-5
18-19	5.1904522254167724E-5	2.673082896089638E-4	0.0	1.6090401898791995E-4	4.4118843916042564E-5
20-21	5.1904522254167724E-5	2.9585577684875604E-4	0.0	2.387608023691715E-4	6.228542670500127E-5
22-23	6.228542670500127E-5	3.218080379758399E-4	0.0	5.553783881195947E-4	6.228542670500127E-5
24-25	8.564246171937675E-5	3.3218894242667346E-4	0.0	0.0016765160688096176	7.007110504312643E-5
26-27	1.0121381839562707E-4	3.373793946520902E-4	0.0	0.0040277909269234155	8.045200949395997E-5
28-29	1.0380904450833545E-4	3.399746207647986E-4	0.0	0.014899193113058845	8.304723560666836E-5
30-31	1.1418994895916899E-4	3.840934646808412E-4	0.0	0.020066288303461244	8.304723560666836E-5
32-33	1.1938040118458577E-4	3.9187914301896633E-4	0.0	0.03169809174062023	8.304723560666836E-5
34-35	1.4792788842437802E-4	4.723311525129263E-4	0.0	0.04567078913144218	8.823768783208513E-5
36-37	1.7907060177687863E-4	5.501879358941779E-4	0.0	0.07154519347514479	9.083291394479352E-5
38-39	1.8166582788958703E-4	5.657592925704282E-4	0.0	0.23501848631464606	9.34281400575019E-5
40-41	2.0242763679125412E-4	6.643778848533469E-4	0.0	0.40448934670061637	9.34281400575019E-5
42-43	2.0242763679125412E-4	7.007110504312643E-4	0.0	0.4574423403043183	9.60233661702103E-5
44-45	2.1280854124208767E-4	7.915439643760578E-4	0.0	0.5321952332547706	9.861859228291868E-5
46-47	2.2318944569292123E-4	8.460437127429339E-4	0.0	0.6156499193611342	9.861859228291868E-5
48-49	2.2318944569292123E-4	8.486389388556423E-4	0.0	0.6904495263816153	9.861859228291868E-5
50-51	2.2318944569292123E-4	9.239004961241854E-4	0.0	0.7621867665891006	9.861859228291868E-5
52-53	2.3357035014375476E-4	9.34281400575019E-4	0.0	1.2641268538219168	9.861859228291868E-5
54-55	2.3876080236917154E-4	9.420670789131442E-4	0.0	1.9309053228295188	9.861859228291868E-5
56-57	2.413560284818799E-4	9.498527572512693E-4	0.0	2.6579345612697587	1.0640427062104384E-4
58-59	2.4395125459458831E-4	9.550432094766861E-4	0.0	3.4167760813995782	1.1418994895916899E-4
60-61	2.4914170682000506E-4	9.835906967164782E-4	0.0	3.628214343254156	1.1418994895916899E-4
62-63	2.4914170682000506E-4	9.913763750546035E-4	0.0	3.6379723934379395	1.1418994895916899E-4
64-65	2.5433215904542187E-4	0.0010899949673375223	0.0	3.645649072279331	1.1418994895916899E-4
66-67	2.595226112708386E-4	0.001095185419562939	0.0	3.651620687564673	1.1678517507187738E-4
68-69	2.595226112708386E-4	0.0011341138112535647	0.0	3.6571485191847417	1.2197562729729416E-4
70-71	2.62117837383547E-4	0.0011444947157043983	0.0	3.66219623397396	1.271660795227109E-4
72-73	2.647130634962554E-4	0.0011496851679298151	0.0	3.6662006678658687	1.297613056354193E-4
74-75	2.647130634962554E-4	0.0011652565246060656	0.0	3.669579652264615	1.323565317481277E-4
76-77	2.647130634962554E-4	0.0012171610468602332	0.0	3.6728574228449657	1.349517578608361E-4
78-79	2.647130634962554E-4	0.0012353276296491918	0.0	3.6749621512223722	1.349517578608361E-4
80-81	2.647130634962554E-4	0.0012379228557619002	0.0	3.675670647951142	1.4533266231166962E-4
82-83	2.724987418343806E-4	0.0012483037602127338	0.0	3.676226026339261	1.5571356676250317E-4
84-85	2.8028442017250573E-4	0.0012508989863254422	0.0	3.6765348582466735	1.5571356676250317E-4
86-87	2.8028442017250573E-4	0.0012794464735652344	0.0	3.6766594291000834	1.6090401898791995E-4
88	2.854748723979225E-4	0.0013391366741575272	0.0	3.676682786135098	1.6609447121333673E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23855	0.0	47.55813	1
ATCTCGT	15545	0.0	45.37972	94
TATCTCG	19480	0.0	34.076134	94
GTATCAA	41180	0.0	33.943775	1
CCGTCTT	8795	0.0	30.707289	90-91
TATGCCG	8580	0.0	30.394154	86-87
GCCGTCT	9460	0.0	29.684147	90-91
ATGCCGT	9355	0.0	29.276209	88-89
TGCCGTC	9485	0.0	27.87387	88-89
TCAACGC	50735	0.0	26.798935	4
TCGTATG	10175	0.0	26.64916	84-85
CTCGTAT	9615	0.0	26.616957	82-83
ATCAACG	51160	0.0	26.584576	3
CAACGCA	51780	0.0	26.242165	5
TCTCGTA	10155	0.0	25.803576	82-83
AACGCAG	52990	0.0	25.675062	6
TATCAAC	53975	0.0	25.450214	2
CGCTATC	29950	0.0	24.894552	94
CTATCTC	30700	0.0	24.476675	94
CGTATGC	11045	0.0	23.831755	84-85
>>END_MODULE