FastQCFastQC Report
Fri 27 May 2016
SRR2139192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13763308
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157350.051.6458931
GTATCAA286400.034.7704961
TCAACGC369200.026.5539954
ATCAACG373200.026.2916983
CAACGCA379400.025.8401035
TATCAAC386450.025.585452
AACGCAG388050.025.2855596
ACGCAGA450150.021.7973167
GTACATG420600.021.3700791
TACATGG427750.020.759822
ACATGGG431450.020.3395233
CGCAGAG497000.019.7425778
ATACAAC126100.019.4446836
AGAGTAC481450.018.42175510-11
GTGGTAT78700.018.4088751
TATACAA154050.018.3758685
TGGTATC70750.018.359082
TATATAC174850.018.2374363
GCAGAGT532600.018.1962769
CATGGGG332650.017.8581774
Produced by FastQC (version 0.11.5)