##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13763308
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.867243834113136	31.0	31.0	34.0	30.0	34.0
2	32.009523800528186	33.0	31.0	34.0	30.0	34.0
3	32.06855394066601	33.0	31.0	34.0	30.0	34.0
4	35.76751759097449	37.0	35.0	37.0	35.0	37.0
5	35.62529139070345	37.0	35.0	37.0	33.0	37.0
6	35.628292122794896	37.0	35.0	37.0	33.0	37.0
7	35.5041654956788	37.0	35.0	37.0	33.0	37.0
8	35.4998033902896	37.0	35.0	37.0	33.0	37.0
9	37.107912283878264	39.0	37.0	39.0	33.0	39.0
10-11	36.97954568770822	39.0	37.0	39.0	33.0	39.0
12-13	36.93179201540792	39.0	37.0	39.0	33.0	39.0
14-15	38.08810781535951	40.0	37.0	41.0	33.0	41.0
16-17	38.08425779616354	40.0	37.0	41.0	33.0	41.0
18-19	38.11265162415896	40.0	37.0	41.0	33.0	41.0
20-21	38.0952955367669	40.0	37.0	41.0	33.0	41.0
22-23	37.99993995298566	40.0	37.0	41.0	33.0	41.0
24-25	37.89802590309252	40.0	37.0	41.0	32.5	41.0
26-27	37.7241141567807	40.0	37.0	41.0	32.0	41.0
28-29	37.58769017657771	39.5	37.0	41.0	32.0	41.0
30-31	37.444333358878986	39.0	36.0	41.0	32.0	41.0
32-33	37.59196662610884	39.5	37.0	41.0	32.0	41.0
34-35	37.54850911532675	40.0	36.0	41.0	32.0	41.0
36-37	37.41052932251007	39.5	36.0	41.0	31.5	41.0
38-39	37.24404078061314	39.0	36.0	41.0	31.0	41.0
40-41	37.17403864801871	39.0	36.0	40.5	31.0	41.0
42-43	37.05701051030675	39.0	35.5	40.0	31.0	41.0
44-45	37.11922028766428	39.0	35.5	40.5	31.0	41.0
46-47	37.268366242059244	39.0	36.0	41.0	31.0	41.0
48-49	37.1815468257503	39.0	36.0	41.0	31.0	41.0
50-51	37.02642902585773	39.0	35.0	41.0	31.0	41.0
52-53	36.87014982419845	39.0	35.0	40.0	30.5	41.0
54-55	36.726755730756466	39.0	35.0	40.0	30.0	41.0
56-57	36.56533846258087	38.0	35.0	40.0	30.0	41.0
58-59	36.409094071598034	38.0	35.0	40.0	30.0	41.0
60-61	36.150566751530754	38.0	34.0	40.0	29.5	41.0
62-63	35.87355597293944	37.5	34.0	40.0	29.0	41.0
64-65	35.62951137093425	37.0	34.0	40.0	29.0	41.0
66-67	35.349096849355234	36.5	34.0	39.0	29.0	41.0
68-69	35.0539470186645	36.0	34.0	39.0	28.5	40.5
70-71	34.74252712003114	35.5	33.0	39.0	28.0	40.0
72-73	34.42470413205619	35.0	33.0	38.0	28.0	40.0
74-75	34.09982115843649	35.0	33.0	37.0	28.0	39.5
76-77	33.00326016612449	34.0	31.5	36.0	26.0	39.0
78-79	33.41013485117996	35.0	32.0	36.0	27.0	39.0
80-81	33.31290670642433	35.0	32.0	36.0	27.0	38.0
82-83	33.09608008552714	35.0	32.0	35.5	27.0	37.0
84-85	32.90794574322605	34.0	32.0	35.0	27.0	37.0
86-87	32.71768867523506	34.0	32.0	35.0	27.0	36.0
88-89	32.507652525149496	34.0	32.0	35.0	26.5	36.0
90-91	32.38482379488084	34.0	31.0	35.0	26.0	36.0
92-93	32.31455818937165	34.0	31.0	35.0	26.5	35.0
94-95	32.292285823767244	34.0	31.0	35.0	27.0	35.0
96-97	32.211430151644635	34.0	31.0	35.0	26.5	35.0
98-99	32.379052901667094	34.0	31.0	35.0	27.0	35.0
100-101	31.616027142109417	33.0	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	1.0
17	1.0
18	5.0
19	23.0
20	153.0
21	801.0
22	3722.0
23	12329.0
24	31614.0
25	66190.0
26	115014.0
27	177341.0
28	248689.0
29	325211.0
30	404462.0
31	495647.0
32	604394.0
33	752468.0
34	978403.0
35	1316829.0
36	1902400.0
37	2691919.0
38	2504153.0
39	1092546.0
40	38991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.83596152901613	21.591429909146843	15.109928514278689	24.462680047558333
2	14.481213731608545	22.26523658444166	40.16772694889756	23.085822735052236
3	17.3678958575947	26.82413268670584	31.214392644559	24.593578811140464
4	12.44366543275788	18.83262366867035	38.20825632907437	30.51545456949739
5	13.07378284348501	37.9707916149228	35.775243858525876	13.180181683066309
6	30.148304462851517	38.40499682198495	18.28582198407534	13.160876731088194
7	26.19886149463487	32.62309468043584	23.53005541981622	17.647988405113075
8	24.696105035213918	36.912448664231015	20.73500789199806	17.656438408557012
9	25.548414668915353	16.04133977093298	21.0692153368943	37.34103022325738
10-11	24.47915137843315	26.675618245264875	28.825097861647798	20.02013251465418
12-13	25.097752662368666	24.35701504318584	28.858814319929483	21.686417974516008
14-15	22.206688973319498	25.563785246976963	26.363680155962506	25.865845623741034
16-17	21.9328739863992	28.672979635419043	26.943500792106086	22.450645586075673
18-19	22.06095227355837	27.815501711690654	28.527017480482325	21.596528534268653
20-21	22.93343931415806	26.889903371820772	28.424559290429364	21.752098023591802
22-23	22.67806002789536	26.89949984929528	28.222623657351804	22.199816465457552
24-25	22.466336563091026	27.068554993751633	28.365797564994526	22.09931087816282
26-27	22.35193339696256	27.07273606349879	28.330631000356572	22.24469953918207
28-29	22.450112870070473	27.09112796044187	28.277673984884515	22.181085184603145
30-31	22.7047031010094	26.833197699781298	28.171931836031245	22.29016736317806
32-33	22.566367057734478	27.017756657568277	28.0848280348786	22.33104824981865
34-35	22.668861771417863	27.10411322593626	28.076638136145938	22.150386866499943
36-37	22.78192293701598	26.99035026585325	27.89069482287322	22.33703197425755
38-39	22.43773577960436	27.243125697742133	28.00866333850673	22.31047518414678
40-41	22.60372931391804	26.99151663813951	28.019802924259235	22.38495112368321
42-43	22.4643304821082	27.232201415984758	27.698273947097558	22.605194154809485
44-45	22.469795390349574	27.23954000437226	27.596640711869952	22.694023893408215
46-47	22.670466544876685	27.17779606294457	27.41503263825336	22.73670475392538
48-49	22.71754162498711	27.443657352585028	27.17688226073964	22.66191876168823
50-51	22.668337788085775	27.39843242996925	27.18934190657119	22.743887875373783
52-53	22.907781159371492	27.29597304813891	27.109793054951275	22.68645273753832
54-55	22.864824342911767	27.2352359122564	27.085076461682313	22.81486328314952
56-57	22.871607009264306	27.457322976991165	26.9139993906606	22.75707062308393
58-59	22.734901113473644	27.4522937269049	27.12562013736867	22.68718502225278
60-61	22.889349382750325	27.447601398771827	26.95985683083005	22.7031923876478
62-63	22.86114830918285	27.46391217392618	27.055819902861238	22.619119614029728
64-65	22.89901727331157	27.343475734380213	26.98228567275189	22.775221319556326
66-67	22.950851007813185	27.39403551583532	26.81264233482667	22.84247114152483
68-69	22.877870599433827	27.303345179054162	26.907051131058868	22.911733090453144
70-71	22.900122386102517	27.145851075034294	26.94121844834386	23.012808090519325
72-73	22.8774340756856	27.226021836241394	26.805747528853097	23.09079655921991
74-75	22.71520107125759	27.249979349681574	26.892207779231526	23.14261179982931
76-77	22.854369699185238	27.247228295603005	26.86335406573478	23.035047939476982
78-79	22.702578562113988	27.25770511923496	26.90006764279953	23.13964867585152
80-81	22.842601433446003	27.163669538658556	26.949686781943	23.044042245952436
82-83	22.918288529645956	27.059380687517464	27.13684141525961	22.88548936757697
84-85	22.850776654130563	27.09289254876684	27.048290122498216	23.008040674604384
86-87	22.72070266862853	27.231280544190916	27.00453598219248	23.04348080498807
88-89	22.87049851344671	27.01900827031109	26.909793863936653	23.20069935230555
90-91	22.858932784159126	27.07262328288086	26.998491159892595	23.069952773067413
92-93	22.884110528331572	26.987767471105116	27.132405507583336	22.995716492979973
94-95	22.528144687339	27.12914162727731	27.249431502300492	23.093282183083193
96-97	22.473438323509427	27.110982818119577	27.313836281179366	23.10174257719163
98-99	22.73881934138948	27.812715265459843	27.305937137742113	22.142528255408564
100-101	23.11879899420733	30.570387146963874	24.55804275549526	21.752771103333536
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3679.0
1	3144.0
2	3856.5
3	5326.0
4	6836.0
5	10514.0
6	13930.0
7	17137.0
8	19713.0
9	20022.5
10	18671.0
11	17230.5
12	18860.5
13	24553.0
14	37958.5
15	56778.0
16	71756.5
17	79049.5
18	79903.5
19	76895.0
20	72064.5
21	67381.0
22	66094.5
23	71563.0
24	84046.0
25	104615.5
26	130717.5
27	157800.5
28	189328.0
29	224352.5
30	256461.0
31	285851.0
32	312561.0
33	337088.5
34	359572.5
35	378688.0
36	399533.0
37	416304.5
38	427455.0
39	439397.5
40	450785.0
41	461275.5
42	469433.5
43	478794.5
44	491580.5
45	512711.0
46	537427.5
47	561244.5
48	577996.5
49	581481.0
50	576382.5
51	572555.0
52	554283.5
53	522291.0
54	500926.0
55	478727.5
56	453015.5
57	421392.0
58	381590.5
59	332751.5
60	283359.5
61	241973.5
62	199825.5
63	162302.5
64	131535.0
65	104429.0
66	83038.0
67	60377.0
68	40890.5
69	30834.5
70	22534.0
71	15273.5
72	10667.0
73	7840.5
74	6106.0
75	4529.0
76	2973.5
77	1698.5
78	1248.5
79	1037.0
80	711.5
81	468.5
82	368.0
83	243.5
84	142.0
85	89.5
86	63.0
87	51.5
88	36.0
89	25.5
90	19.5
91	15.0
92	11.5
93	5.5
94	2.5
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0033640168482751386
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.089854270499505E-4
20-21	0.0017765690165273598
22-23	3.6336146417228707E-4
24-25	2.5443463207189246E-5
26-27	3.0911417695804737E-4
28-29	0.0034828264086167672
30-31	3.7901932685147115E-4
32-33	6.576616707763215E-5
34-35	0.0023681290303329103
36-37	0.05557585953805587
38-39	0.03225743989139896
40-41	0.041409695318233825
42-43	8.478427489096741E-4
44-45	0.002320758323380704
46-47	0.0014502260679328206
48-49	5.755128908665793E-4
50-51	7.856705631474466E-6
52-53	9.676332622347609E-4
54-55	0.002270177736098685
56-57	0.0012097627938383341
58-59	0.003726658020220086
60-61	0.001398537105361689
62-63	8.78886067990124E-5
64-65	7.535054619198023E-4
66-67	3.9429406768717683E-4
68-69	1.9972707729077515E-4
70-71	0.001126712311132098
72-73	0.0016817827110863698
74-75	6.284348599236783E-4
76-77	0.001003732463957411
78-79	1.7666129492357262E-4
80-81	0.0018888113426898752
82-83	0.0024472806561130645
84-85	0.003568846833918496
86-87	0.003689266930759997
88-89	0.06404098299625523
90-91	6.858785901038446E-4
92-93	5.278372386427108E-5
94-95	5.41968055102109E-5
96-97	0.007700356710178457
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	497.0
20-21	1714.0
22-23	3295.0
24-25	6862.0
26-27	8877.0
28-29	14823.0
30-31	32800.0
32-33	41739.0
34-35	58475.0
36-37	90013.0
38-39	101703.0
40-41	105219.0
42-43	120804.0
44-45	141999.0
46-47	143136.0
48-49	132525.0
50-51	121415.0
52-53	117991.0
54-55	128076.0
56-57	134664.0
58-59	137691.0
60-61	138987.0
62-63	133997.0
64-65	141547.0
66-67	150786.0
68-69	154282.0
70-71	154184.0
72-73	148727.0
74-75	150497.0
76-77	150228.0
78-79	164560.0
80-81	170248.0
82-83	164134.0
84-85	169116.0
86-87	181365.0
88-89	197389.0
90-91	219538.0
92-93	235557.0
94-95	306833.0
96-97	385693.0
98-99	1202085.0
100-101	7399237.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.86888285721083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.58443309078162	75.13346324720405
2	7.0262790155543655	11.785723433552155
3	1.7794451748014986	4.477202367487678
4	0.7171846042735572	2.405978862512563
5	0.3381011734946486	1.41780838568541
6	0.1820468214733026	0.9160838126803192
7	0.1042686728873057	0.6121427978443593
8	0.06811746296661629	0.4570348417662032
9	0.0416590630727963	0.31445091727141594
>10	0.15263909283765267	2.036519123240997
>50	0.0044305944593375605	0.2519376836764252
>100	0.001378965912012496	0.18289549114028988
>500	1.6267485262998857E-5	0.008759035938102747
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1797085409990097E-5	0.0	7.2656951366633664E-6	0.0
3	0.0	2.1797085409990097E-5	0.0	1.4531390273326733E-5	0.0
4	0.0	8.718834163996039E-5	0.0	2.9062780546653466E-5	0.0
5	0.0	9.445403677662375E-5	0.0	3.632847568331683E-5	0.0
6	0.0	9.445403677662375E-5	0.0	4.3594170819980194E-5	0.0
7	7.2656951366633664E-6	9.445403677662375E-5	0.0	4.3594170819980194E-5	0.0
8	7.2656951366633664E-6	1.089854270499505E-4	0.0	5.0859865956643566E-5	7.2656951366633664E-6
9	1.4531390273326733E-5	2.542993297832178E-4	0.0	7.992264650329702E-5	7.2656951366633664E-6
10-11	1.4531390273326733E-5	2.542993297832178E-4	0.0	1.089854270499505E-4	7.2656951366633664E-6
12-13	1.4531390273326733E-5	2.5793217735154946E-4	0.0	1.5621244543826237E-4	7.2656951366633664E-6
14-15	1.8164237841658414E-5	3.3422197628651484E-4	0.0	1.6711098814325742E-4	7.2656951366633664E-6
16-17	2.1797085409990097E-5	3.378548238548465E-4	0.0	1.707438357115891E-4	7.2656951366633664E-6
18-19	2.542993297832178E-5	3.814489946748267E-4	0.0	2.2886939680489605E-4	1.089854270499505E-5
20-21	2.9062780546653466E-5	4.286760130631386E-4	0.0	2.906278054665346E-4	1.4531390273326733E-5
22-23	2.9062780546653466E-5	4.650044887464554E-4	0.0	3.196905860131881E-4	1.4531390273326733E-5
24-25	2.9062780546653466E-5	5.485599828180842E-4	0.0	3.4875336655984155E-4	1.4531390273326733E-5
26-27	3.2695628114985145E-5	5.812556109330692E-4	0.0	3.9234753737982177E-4	1.4531390273326733E-5
28-29	3.632847568331683E-5	5.812556109330692E-4	0.0	7.846950747596435E-4	1.8164237841658414E-5
30-31	4.3594170819980194E-5	6.829753428463565E-4	0.0	0.0016420471008859207	2.1797085409990097E-5
32-33	4.3594170819980194E-5	6.902410379830198E-4	0.0	0.0034657365801884256	2.1797085409990097E-5
34-35	4.3594170819980194E-5	8.210235504429604E-4	0.0	0.006175840866163861	2.1797085409990097E-5
36-37	4.3594170819980194E-5	9.372746726295742E-4	0.0	0.009805055586927212	2.1797085409990097E-5
38-39	4.3594170819980194E-5	9.663374531762277E-4	0.0	0.015817418312516148	2.1797085409990097E-5
40-41	4.3594170819980194E-5	0.0010535257948161882	0.0	0.022894205375626267	2.1797085409990097E-5
42-43	5.812556109330693E-5	0.00107895572779451	0.0	0.029262587162911705	2.1797085409990097E-5
44-45	6.902410379830198E-5	0.0011915740024127921	0.0	0.03694605976993322	2.9062780546653466E-5
46-47	7.265695136663366E-5	0.001282395191621084	0.0	0.045755715123137544	2.9062780546653466E-5
48-49	7.265695136663366E-5	0.0013005594294627425	0.0	0.054398259488198625	2.9062780546653466E-5
50-51	7.265695136663366E-5	0.001387747771102703	0.0	0.0627901373710448	2.9062780546653466E-5
52-53	7.992264650329702E-5	0.0013950134662393662	0.0	0.07110572545495603	2.9062780546653466E-5
54-55	7.992264650329702E-5	0.0014059120089443614	0.0	0.08019874291849023	2.9062780546653466E-5
56-57	7.992264650329702E-5	0.0014531390273326732	0.0	0.08926996329661444	3.632847568331683E-5
58-59	7.992264650329702E-5	0.0014640375700376682	0.0	0.09857731876668022	4.3594170819980194E-5
60-61	7.992264650329702E-5	0.0014967331981526534	0.0	0.10698736088736807	4.3594170819980194E-5
62-63	8.718834163996039E-5	0.0015185302835626436	0.0	0.11481614739712284	4.3594170819980194E-5
64-65	8.718834163996039E-5	0.0016929069668425642	0.0	0.12308450846264575	4.3594170819980194E-5
66-67	9.445403677662377E-5	0.0017219697473892178	0.0	0.13168345865688685	5.0859865956643566E-5
68-69	1.0535257948161882E-4	0.0017873610036191882	0.0	0.1412741762372825	5.0859865956643566E-5
70-71	1.089854270499505E-4	0.0018055252414608464	0.0	0.15044348349975167	5.0859865956643566E-5
72-73	1.089854270499505E-4	0.00181279093659751	0.0	0.1581451203446148	5.0859865956643566E-5
74-75	1.1625112218661386E-4	0.0018563851074174902	0.0	0.16556339507914813	5.8125561093306925E-5
76-77	1.1625112218661386E-4	0.0019508391441941137	0.0	0.17259658797143826	6.539125622997029E-5
78-79	1.1625112218661386E-4	0.0019980661625824256	0.0	0.17883055439869544	6.539125622997029E-5
80-81	1.1625112218661386E-4	0.0019980661625824256	0.0	0.18318997148069344	6.902410379830198E-5
82-83	1.1625112218661386E-4	0.0020053318577190893	0.0	0.18687367891498177	7.265695136663366E-5
84-85	1.1625112218661386E-4	0.002019863247992416	0.0	0.1901105460983653	7.265695136663366E-5
86-87	1.2351681732327723E-4	0.0020343946382657425	0.0	0.19065547323361504	7.265695136663366E-5
88-89	1.2351681732327723E-4	0.0020997858944957126	0.0	0.19078262289850667	7.265695136663366E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15735	0.0	51.645893	1
GTATCAA	28640	0.0	34.770496	1
TCAACGC	36920	0.0	26.553995	4
ATCAACG	37320	0.0	26.291698	3
CAACGCA	37940	0.0	25.840103	5
TATCAAC	38645	0.0	25.58545	2
AACGCAG	38805	0.0	25.285559	6
ACGCAGA	45015	0.0	21.797316	7
GTACATG	42060	0.0	21.370079	1
TACATGG	42775	0.0	20.75982	2
ACATGGG	43145	0.0	20.339523	3
CGCAGAG	49700	0.0	19.742577	8
ATACAAC	12610	0.0	19.444683	6
AGAGTAC	48145	0.0	18.421755	10-11
GTGGTAT	7870	0.0	18.408875	1
TATACAA	15405	0.0	18.375868	5
TGGTATC	7075	0.0	18.35908	2
TATATAC	17485	0.0	18.237436	3
GCAGAGT	53260	0.0	18.196276	9
CATGGGG	33265	0.0	17.858177	4
>>END_MODULE