FastQCFastQC Report
Fri 27 May 2016
SRR2139191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21517715
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA283450.050.623981
GTATCAA512200.034.5357321
TCAACGC637700.027.0821274
ATCAACG648650.026.6692283
CAACGCA653900.026.423735
AACGCAG663100.026.094316
TATCAAC671950.025.9461332
ACGCAGA758950.022.7501267
CGCAGAG818200.021.1326688
GTACATG655850.020.3005921
TACATGG666400.019.5107042
ACATGGG657050.019.440213
GCAGAGT888100.019.266539
GAGTACT539750.018.51895512-13
GTGGTAT133300.018.367511
AGAGTAC814500.018.19398710-11
CAGAGTA877850.017.85793310-11
CATGGGG473950.017.2282354
TATATAC288700.017.1972433
TGGTATC129900.016.9507872
Produced by FastQC (version 0.11.5)