##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139191_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21517715
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.571290910768173	31.0	31.0	34.0	30.0	34.0
2	31.692326531883147	31.0	31.0	34.0	30.0	34.0
3	31.7531442348781	31.0	31.0	34.0	30.0	34.0
4	35.532157898735996	37.0	35.0	37.0	33.0	37.0
5	35.37407020215669	37.0	35.0	37.0	33.0	37.0
6	35.39961348126416	37.0	35.0	37.0	33.0	37.0
7	35.29724461914288	37.0	35.0	37.0	32.0	37.0
8	35.280162972694825	37.0	35.0	37.0	32.0	37.0
9	36.86529536244903	39.0	37.0	39.0	33.0	39.0
10-11	36.735989973842486	39.0	35.0	39.0	32.0	39.0
12-13	36.697328410567756	39.0	35.0	39.0	32.0	39.0
14-15	37.81379879322688	40.0	37.0	41.0	32.0	41.0
16-17	37.77284846462554	40.0	37.0	41.0	32.0	41.0
18-19	37.80896096541849	40.0	37.0	41.0	32.0	41.0
20-21	37.791010032547646	40.0	37.0	41.0	32.0	41.0
22-23	37.69531239926298	40.0	37.0	41.0	32.0	41.0
24-25	37.62610184908539	39.0	36.0	41.0	32.0	41.0
26-27	37.42781267324359	39.0	36.0	41.0	31.5	41.0
28-29	37.27407258373597	39.0	36.0	41.0	31.0	41.0
30-31	37.114475182723325	39.0	36.0	41.0	31.0	41.0
32-33	36.88963578885114	39.0	35.5	40.0	30.0	41.0
34-35	36.76201755529742	39.0	35.0	40.0	30.0	41.0
36-37	36.61175412472257	38.0	35.0	40.0	30.0	41.0
38-39	36.48098861598037	38.0	35.0	40.0	30.0	41.0
40-41	36.35284392656759	38.0	35.0	40.0	30.0	41.0
42-43	36.11519166434017	38.0	35.0	40.0	29.5	41.0
44-45	35.95044119508228	38.0	34.0	40.0	29.0	41.0
46-47	35.960095754983605	38.0	34.0	40.0	29.0	41.0
48-49	36.240424573966564	38.0	34.5	40.0	29.5	41.0
50-51	36.53062632503516	39.0	35.0	40.0	30.0	41.0
52-53	36.50955658367862	39.0	35.0	40.0	30.0	41.0
54-55	36.41724146800256	38.0	35.0	40.0	29.5	41.0
56-57	36.28239672253972	38.0	34.5	40.0	29.0	41.0
58-59	36.1211955715955	38.0	34.0	40.0	29.0	41.0
60-61	35.93175756591613	38.0	34.0	40.0	28.5	41.0
62-63	35.73102186795295	37.5	34.0	40.0	28.0	41.0
64-65	35.48501954798328	37.0	34.0	40.0	28.0	41.0
66-67	35.23522404325162	36.5	33.5	39.5	28.0	41.0
68-69	34.966150240079585	36.0	33.0	39.0	28.0	41.0
70-71	34.68709525971849	36.0	33.0	39.0	28.0	40.5
72-73	34.41923225679837	35.0	33.0	39.0	28.0	40.0
74-75	34.117283378472976	35.0	32.0	38.0	27.0	40.0
76-77	32.402926431661754	34.0	30.5	36.0	25.5	39.0
78-79	33.41116131347407	35.0	32.0	37.0	26.5	39.0
80-81	33.46914693211686	35.0	32.0	36.0	27.0	39.0
82-83	33.26855833572356	35.0	32.0	36.0	27.0	38.0
84-85	33.041033033160176	35.0	32.0	36.0	27.0	37.0
86-87	32.84425845428772	34.0	32.0	35.0	27.0	37.0
88-89	32.696714221625456	34.0	32.0	35.0	27.0	36.0
90-91	32.582117204782875	34.0	32.0	35.0	27.0	36.0
92-93	32.50629009208819	34.0	32.0	35.0	27.0	36.0
94-95	32.490146932979115	34.0	32.0	35.0	27.0	35.5
96-97	32.59089093933715	34.0	32.0	35.0	27.0	35.0
98-99	32.72072162285528	34.0	32.0	35.0	28.5	35.0
100	31.486750684642697	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	65.0
20	295.0
21	1911.0
22	8468.0
23	28270.0
24	70463.0
25	140969.0
26	239718.0
27	360264.0
28	488669.0
29	621092.0
30	753621.0
31	897651.0
32	1065626.0
33	1283580.0
34	1583892.0
35	2014572.0
36	2689036.0
37	3609453.0
38	3727555.0
39	1863730.0
40	68806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.2337051122761	21.686800852228036	14.85117727416689	23.22831676132898
2	14.643279755516996	22.46361586474566	41.15270017287739	21.740404206859953
3	17.657655564264143	27.62071158577944	31.520977018238227	23.20065583171819
4	12.399996932759821	19.182366715053156	39.4690142517456	28.94862210044143
5	12.450852704387989	38.594209468802795	36.418086214079885	12.536851612729324
6	28.688524780628427	39.62007583054242	18.968566132602835	12.722833256226323
7	25.10584315564014	33.42736914858139	24.450995850483565	17.015791845294903
8	23.835834799373444	37.600107632246264	21.427651588470244	17.13640597991004
9	25.173114338581026	16.521912294126025	21.907753681094853	36.3972196861981
10-11	23.681227242431923	27.271326734689566	29.594162797430368	19.453283225448143
12-13	24.42813668695887	25.045435253732308	29.60297698999766	20.92345106931117
14-15	21.59969987226679	26.328326634523847	27.21536720916296	24.85660628404641
16-17	21.109725789510545	29.475999884188187	27.760100572241402	21.654173754059862
18-19	21.157456688553307	28.519797311003803	29.515982078935497	20.806763921507393
20-21	22.05111122993765	27.611879583415224	29.451177315774924	20.885831870872206
22-23	21.809544110366964	27.60243054550793	29.248409228553452	21.33961611557166
24-25	21.604086059367894	27.79176203288457	29.331911919793697	21.27223998795384
26-27	21.508898209087516	27.807894293600494	29.28496379972487	21.398243697587116
28-29	21.605798174795314	27.842324859418248	29.244863191162068	21.307013774624366
30-31	21.95527005421084	27.588819447962408	29.09825636883392	21.35765412899283
32-33	21.699855846282112	27.79492012965289	28.997608255007645	21.507615769057356
34-35	21.84999931707659	27.881531853863418	28.986285651981337	21.282183177078657
36-37	21.910736976233142	27.77868497453816	28.8493266002419	21.4612514489868
38-39	21.611615533534557	27.99774526220852	28.949274424454746	21.441364779802175
40-41	21.781735181552406	27.799801142485673	28.90960580836281	21.50885786759911
42-43	21.767540499616615	27.969148774039514	28.520553268435155	21.742757457908713
44-45	21.678047660217448	28.078079509953373	28.391995037599333	21.851877792229846
46-47	21.89010610873291	28.00417532969478	28.193296737313823	21.91242182425849
48-49	21.944259700147157	28.231844965409692	27.97727287681755	21.846622457625607
50-51	21.90083440740646	28.23135546586057	27.952558005723578	21.91525212100939
52-53	22.119583956125034	28.156389967853112	27.889577605220435	21.83444847080142
54-55	22.107399903143524	28.072900198552663	27.89124767121113	21.92845222709268
56-57	22.12933619198212	28.270292104386456	27.6585691731242	21.94180253050723
58-59	21.971205314816565	28.25669993683249	27.8963019245353	21.875792823815647
60-61	22.11097448842115	28.287834330489463	27.70953526259875	21.89165591849064
62-63	22.080797063101016	28.27934379114454	27.82511389187437	21.814745253880073
64-65	22.133223118752735	28.183480923584426	27.754627908848526	21.928668048814313
66-67	22.188458450376874	28.199322648731485	27.565675049319534	22.04654385157211
68-69	22.102112903948854	28.134807983615012	27.680371380102176	22.08270773233396
70-71	22.152455429198675	27.935175260092944	27.72679006663818	22.185579244070205
72-73	22.16142512165237	28.0345113504125	27.582575068817082	22.221488459118046
74-75	21.99622921667418	28.049705497283245	27.664814549327037	22.289250736715537
76-77	22.0942693636982	28.09631681054603	27.663729063504434	22.14568476225134
78-79	21.955432206046556	28.089339228938474	27.67580057890338	22.279427986111585
80-81	22.086882279712437	27.969817861605534	27.760925832044673	22.18237402663735
82-83	22.23800716476641	27.861705331570263	27.903024342849324	21.997263160813997
84-85	22.193158619942086	27.891780236111657	27.81316668137716	22.101894462569096
86-87	21.967939101578324	28.077268300596636	27.827419708135515	22.12737288968953
88-89	22.180381312375427	27.84244631671689	27.715531452021114	22.26164091888657
90-91	22.183927522181115	27.857496148209428	27.815224091598402	22.143352238011055
92-93	22.230705516723663	27.787348639993816	27.936846302986385	22.045099540296142
94-95	21.887249320111067	27.908816504290872	28.078147001206027	22.125787174392034
96-97	21.901194188151916	28.00940485283946	28.142407695583334	21.946993263425295
98-99	22.999099888812637	30.035603565424978	28.313103964594738	18.65219258116764
100	22.224469101552764	30.738635551330507	22.160039741056153	24.87685560606058
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5470.0
1	4792.0
2	6502.5
3	9351.0
4	12183.5
5	19764.5
6	26988.0
7	32951.5
8	37537.0
9	37459.5
10	33708.0
11	29645.0
12	31218.5
13	40389.0
14	63387.5
15	95973.0
16	123195.5
17	139097.5
18	144376.5
19	141789.0
20	134293.5
21	125510.5
22	121527.5
23	129468.5
24	151228.0
25	188369.5
26	239076.5
27	295828.0
28	361047.5
29	434965.0
30	503548.0
31	564791.5
32	622077.0
33	671289.0
34	713546.5
35	749129.5
36	784054.5
37	811095.0
38	814487.0
39	808023.0
40	802046.5
41	791378.5
42	778495.5
43	769982.5
44	767555.0
45	779855.0
46	798710.5
47	819947.5
48	826521.5
49	811522.5
50	797366.0
51	783549.5
52	750909.5
53	700556.0
54	657947.0
55	623182.0
56	587913.5
57	542349.5
58	491606.5
59	436176.5
60	373100.5
61	319345.0
62	267278.5
63	217832.5
64	179353.5
65	144853.0
66	116848.5
67	88226.5
68	61088.5
69	44996.5
70	33044.5
71	22645.0
72	16323.0
73	11964.5
74	9024.5
75	6666.5
76	4443.5
77	2677.5
78	2036.0
79	1676.0
80	1166.5
81	762.0
82	560.0
83	352.5
84	201.5
85	136.0
86	98.5
87	71.5
88	46.0
89	27.5
90	21.0
91	20.5
92	18.5
93	12.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11092720579299428
3	0.0
4	0.0
5	0.0
6	0.0
7	1.2547800730700263E-4
8	0.0
9	0.0
10-11	4.8797002841612125E-5
12-13	3.485500202972295E-5
14-15	9.759400568322426E-4
16-17	2.230720129902269E-4
18-19	9.294667207926121E-5
20-21	8.482005066057622E-4
22-23	3.4166660507370725E-4
24-25	3.534222574735089E-4
26-27	0.0021661364789760983
28-29	0.003982360240115362
30-31	0.011238847129933952
32-33	0.008318074945082679
34-35	0.0074093462838614125
36-37	0.003959307958353186
38-39	6.953433073028073E-4
40-41	2.2094696862079929E-4
42-43	3.34577217075929E-4
44-45	2.2063389785866468E-5
46-47	0.0
48-49	3.5160143907455284E-5
50-51	0.0
52-53	0.0014001798333534684
54-55	6.736174313122669E-4
56-57	8.120996136056236E-4
58-59	6.360475327861964E-5
60-61	4.8270018622573185E-4
62-63	4.0697548382398615E-4
64-65	9.168603648566215E-4
66-67	8.590532915794512E-4
68-69	3.212113341504311E-4
70-71	3.1140974448516195E-4
72-73	0.0
74-75	0.0
76-77	2.7983521159322746E-4
78-79	1.1474422184333814E-4
80-81	2.943860217666571E-4
82-83	5.920518472890133E-5
84-85	1.582907987489835E-5
86-87	0.0
88-89	1.6390913585595613E-5
90-91	0.0
92-93	0.0
94-95	9.136276453605303E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	945.0
20-21	3228.0
22-23	6325.0
24-25	13342.0
26-27	17838.0
28-29	29768.0
30-31	70104.0
32-33	80999.0
34-35	108819.0
36-37	145886.0
38-39	176340.0
40-41	189348.0
42-43	218237.0
44-45	246586.0
46-47	245491.0
48-49	216702.0
50-51	200345.0
52-53	197581.0
54-55	208311.0
56-57	219998.0
58-59	221120.0
60-61	219532.0
62-63	211999.0
64-65	223645.0
66-67	239281.0
68-69	243883.0
70-71	241325.0
72-73	232065.0
74-75	238305.0
76-77	229560.0
78-79	251043.0
80-81	260559.0
82-83	251634.0
84-85	256955.0
86-87	274539.0
88-89	303165.0
90-91	333390.0
92-93	361062.0
94-95	426220.0
96-97	894813.0
98-99	1396261.0
100-101	1.1611166E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.91183097250865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.91316894024209	73.71953634403458
2	7.428236303085218	12.317773455705085
3	1.946204227698213	4.84090067894688
4	0.7454995682502695	2.4724293677137807
5	0.35782596816493795	1.48340030950328
6	0.19241277701952045	0.9571977387116084
7	0.11462165844211161	0.6652444099378685
8	0.07815742458085158	0.5184140140875328
9	0.0489228350925266	0.36506536504987325
>10	0.16882092341309474	2.1787926304667034
>50	0.004680565422256387	0.26179000404453767
>100	0.00138238998382252	0.17677061537681865
>500	5.538073344703649E-5	0.030568213697992317
>1k	1.1037871610649158E-5	0.012116852723485947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3236668019815302E-5	0.0	1.394200081188918E-5	0.0
3	0.0	2.3236668019815302E-5	0.0	1.394200081188918E-5	0.0
4	0.0	8.365200487133508E-5	0.0	2.788400162377836E-5	0.0
5	0.0	1.0224133928718732E-4	0.0	2.788400162377836E-5	0.0
6	0.0	1.0224133928718732E-4	0.0	2.788400162377836E-5	4.64733360396306E-6
7	0.0	1.0224133928718732E-4	0.0	2.788400162377836E-5	9.29466720792612E-6
8	0.0	1.0224133928718732E-4	0.0	2.788400162377836E-5	9.29466720792612E-6
9	0.0	2.091300121783377E-4	0.0	3.717866883170448E-5	9.29466720792612E-6
10-11	0.0	2.091300121783377E-4	0.0	3.717866883170448E-5	9.29466720792612E-6
12-13	0.0	2.091300121783377E-4	0.0	6.506267045548285E-5	1.394200081188918E-5
14-15	0.0	2.811636830397651E-4	0.0	7.900467126737201E-5	2.091300121783377E-5
16-17	0.0	2.9742935065363584E-4	0.0	8.829933847529815E-5	2.788400162377836E-5
18-19	0.0	3.090476846635435E-4	0.0	9.527033888124273E-5	4.182600243566754E-5
20-21	0.0	3.229896854754327E-4	0.0	1.0456500608916885E-4	4.64733360396306E-5
22-23	0.0	3.7411035511902634E-4	0.0	1.2780167410898416E-4	6.041533685151978E-5
24-25	0.0	4.1128902395073084E-4	0.0	1.92864344564467E-4	6.97100040594459E-5
26-27	0.0	4.3220202516856457E-4	0.0	3.044003510595804E-4	6.97100040594459E-5
28-29	4.64733360396306E-6	4.3684935877252764E-4	0.0	9.619980560203534E-4	6.97100040594459E-5
30-31	4.64733360396306E-6	5.112066964359366E-4	0.0	0.003050974511001749	6.97100040594459E-5
32-33	4.64733360396306E-6	5.135303632379181E-4	0.0	0.008030592467648168	8.132833806935355E-5
34-35	4.64733360396306E-6	5.855640340993455E-4	0.0	0.01403262381716646	9.294667207926121E-5
36-37	4.64733360396306E-6	6.552740381587914E-4	0.0	0.02024610884566507	9.294667207926121E-5
38-39	4.64733360396306E-6	6.715397057726622E-4	0.0	0.0331261939290487	9.294667207926121E-5
40-41	4.64733360396306E-6	7.598390442479603E-4	0.0	0.041458863080954464	9.527033888124273E-5
42-43	4.64733360396306E-6	7.830757122677756E-4	0.0	0.05368832145978325	9.99176724852058E-5
44-45	4.64733360396306E-6	8.783460511490183E-4	0.0	0.06318282401267979	1.0224133928718732E-4
46-47	4.64733360396306E-6	9.573507224163904E-4	0.0	0.07791487153724269	1.0921233969313191E-4
48-49	4.64733360396306E-6	9.666453896243165E-4	0.0	0.0909088162939234	1.2083067370303957E-4
50-51	4.64733360396306E-6	0.0010502973944956516	0.0	0.10382375637933675	1.2083067370303957E-4
52-53	4.64733360396306E-6	0.0010549447280996146	0.0	0.1144615029988082	1.3477267451492874E-4
54-55	4.64733360396306E-6	0.0010665630621095224	0.0	0.12712548706960752	1.4406734172285488E-4
56-57	4.64733360396306E-6	0.0010874760633273561	0.0	0.13875543941352508	1.4639100852483638E-4
58-59	4.64733360396306E-6	0.0010897997301293377	0.0	0.148326622970887	1.4871467532681792E-4
60-61	4.64733360396306E-6	0.001126978398961042	0.0	0.1568196251321295	1.603330093367256E-4
62-63	4.64733360396306E-6	0.0011432440665749128	0.0	0.16426465356567832	1.7195134334663324E-4
64-65	4.64733360396306E-6	0.0012524564062680447	0.0	0.17063614793671167	1.7195134334663324E-4
66-67	4.64733360396306E-6	0.0012640747402779524	0.0	0.17745146266692352	1.7195134334663324E-4
68-69	4.64733360396306E-6	0.0012942824087037123	0.0	0.18382295703795687	1.7195134334663324E-4
70-71	4.64733360396306E-6	0.001315195409921546	0.0	0.18934631302626698	1.8589334415852242E-4
72-73	4.64733360396306E-6	0.0013175190767235276	0.0	0.1938216952868834	1.998353449704116E-4
74-75	4.64733360396306E-6	0.0013639924127631584	0.0	0.1973699344935092	1.998353449704116E-4
76-77	4.64733360396306E-6	0.0014499680844364748	0.0	0.20069045435354077	1.998353449704116E-4
78-79	4.64733360396306E-6	0.0014801757528622346	0.0	0.20290490881582918	1.998353449704116E-4
80-81	4.64733360396306E-6	0.001482499419664216	0.0	0.20361362719043358	2.021590117723931E-4
82-83	4.64733360396306E-6	0.0014964414204761054	0.0	0.20422010422575074	2.091300121783377E-4
84-85	4.64733360396306E-6	0.0015080597544860131	0.0	0.20449429690838455	2.1145367898031924E-4
86-87	4.64733360396306E-6	0.0015266490889018652	0.0	0.20464533525051334	2.1377734578230078E-4
88	4.64733360396306E-6	0.0015986827597632928	0.0	0.20464533525051334	2.1842467938626382E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28345	0.0	50.62398	1
GTATCAA	51220	0.0	34.535732	1
TCAACGC	63770	0.0	27.082127	4
ATCAACG	64865	0.0	26.669228	3
CAACGCA	65390	0.0	26.42373	5
AACGCAG	66310	0.0	26.09431	6
TATCAAC	67195	0.0	25.946133	2
ACGCAGA	75895	0.0	22.750126	7
CGCAGAG	81820	0.0	21.132668	8
GTACATG	65585	0.0	20.300592	1
TACATGG	66640	0.0	19.510704	2
ACATGGG	65705	0.0	19.44021	3
GCAGAGT	88810	0.0	19.26653	9
GAGTACT	53975	0.0	18.518955	12-13
GTGGTAT	13330	0.0	18.36751	1
AGAGTAC	81450	0.0	18.193987	10-11
CAGAGTA	87785	0.0	17.857933	10-11
CATGGGG	47395	0.0	17.228235	4
TATATAC	28870	0.0	17.197243	3
TGGTATC	12990	0.0	16.950787	2
>>END_MODULE