FastQCFastQC Report
Fri 27 May 2016
SRR2139190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9719021
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109300.040.931731
GTATCAA187950.032.5879021
TCAACGC226050.026.3327564
ATCAACG228900.026.0048923
CAACGCA230350.025.7878425
AACGCAG232300.025.6066426
TATCAAC244200.024.62852
ACGCAGA272250.021.8190257
CGCAGAG280600.021.2135418
GTACATG280050.020.2907681
ACATGGG277150.019.7629493
TACATGG285600.019.6956312
GCAGAGT332300.017.8021349
AGAGTAC300300.017.78924410-11
CATGGGG197900.017.1817554
GAGTACT173150.016.73937212-13
CAGAGTA327650.015.78543210-11
TATACCG7350.015.0491395
AGTACTT209700.014.63250312-13
ATGGGGG115150.014.4799045
Produced by FastQC (version 0.11.5)