##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9719021
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.882038530424	31.0	31.0	34.0	30.0	34.0
2	32.02839071960025	33.0	31.0	34.0	30.0	34.0
3	32.09347968277875	33.0	31.0	34.0	30.0	34.0
4	35.78760978086167	37.0	35.0	37.0	35.0	37.0
5	35.65080258598063	37.0	35.0	37.0	33.0	37.0
6	35.65039369706064	37.0	35.0	37.0	33.0	37.0
7	35.53421419708837	37.0	35.0	37.0	33.0	37.0
8	35.528456415517574	37.0	35.0	37.0	33.0	37.0
9	37.134153018086906	39.0	37.0	39.0	33.0	39.0
10-11	37.01196884953741	39.0	37.0	39.0	33.0	39.0
12-13	36.97342288899263	39.0	37.0	39.0	33.0	39.0
14-15	38.141176462114856	40.0	37.0	41.0	33.0	41.0
16-17	38.14195308354617	40.0	37.0	41.0	33.0	41.0
18-19	38.17238901942902	40.0	37.5	41.0	33.0	41.0
20-21	38.160055607073375	40.0	37.0	41.0	33.0	41.0
22-23	38.0758326569277	40.0	37.0	41.0	33.0	41.0
24-25	37.98051723768113	40.0	37.0	41.0	33.0	41.0
26-27	37.81701804524049	40.0	37.0	41.0	32.5	41.0
28-29	37.6917607711337	40.0	37.0	41.0	32.0	41.0
30-31	37.56118679737394	39.0	37.0	41.0	32.0	41.0
32-33	37.70910220520861	40.0	37.0	41.0	33.0	41.0
34-35	37.67257237077192	40.0	37.0	41.0	32.0	41.0
36-37	37.54260385852407	40.0	36.5	41.0	32.0	41.0
38-39	37.388810931320705	39.0	36.0	41.0	31.0	41.0
40-41	37.329659954894254	39.0	36.0	41.0	31.0	41.0
42-43	37.22940906790632	39.0	36.0	40.5	31.0	41.0
44-45	37.29600417215546	39.0	36.0	40.5	31.5	41.0
46-47	37.45414536755359	40.0	36.0	41.0	31.5	41.0
48-49	37.389259535571355	39.5	36.0	41.0	31.0	41.0
50-51	37.26193286245737	39.0	36.0	41.0	31.0	41.0
52-53	37.13261610011665	39.0	36.0	41.0	31.0	41.0
54-55	37.01139761803321	39.0	35.0	40.5	31.0	41.0
56-57	36.869386075379154	39.0	35.0	40.0	31.0	41.0
58-59	36.7414704203327	39.0	35.0	40.0	31.0	41.0
60-61	36.51593441011923	38.0	35.0	40.0	30.0	41.0
62-63	36.260586242668495	38.0	34.5	40.0	30.0	41.0
64-65	36.0424025079508	38.0	34.0	40.0	29.0	41.0
66-67	35.79069802869762	37.0	34.0	40.0	29.0	41.0
68-69	35.513744090206075	37.0	34.0	39.5	29.0	41.0
70-71	35.217717795697176	36.0	34.0	39.0	29.0	40.5
72-73	34.8996089122904	36.0	33.5	39.0	29.0	40.0
74-75	34.566068318152645	35.0	33.0	38.5	28.5	40.0
76-77	33.44136461311203	34.5	31.5	37.0	26.5	39.0
78-79	33.82862460725747	35.0	32.5	37.0	28.0	39.0
80-81	33.69838627612605	35.0	33.0	36.5	28.0	39.0
82-83	33.446744942790815	35.0	32.0	36.0	28.0	38.5
84-85	33.21692871089641	35.0	32.0	36.0	28.0	37.0
86-87	32.98730628051021	34.5	32.0	35.0	27.0	37.0
88-89	32.74316097898256	34.0	32.0	35.0	27.0	36.0
90-91	32.596424736791306	34.0	32.0	35.0	27.0	36.0
92-93	32.51010638231949	34.0	32.0	35.0	27.0	36.0
94-95	32.46575042950003	34.0	32.0	35.0	27.0	35.5
96-97	32.36385534858202	34.0	31.0	35.0	27.0	35.0
98-99	32.5094205399122	34.0	31.5	35.0	27.5	35.0
100-101	31.728129040371016	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	13.0
20	86.0
21	495.0
22	2386.0
23	7899.0
24	19902.0
25	40752.0
26	72877.0
27	112083.0
28	159188.0
29	210062.0
30	263185.0
31	324322.0
32	398846.0
33	496652.0
34	645617.0
35	859773.0
36	1232032.0
37	1816642.0
38	1971516.0
39	1042503.0
40	42187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.52734971968884	23.251313069495374	15.777545907144352	23.44379130367143
2	13.430466331820853	22.962037523117797	41.87648720991754	21.73100893514381
3	16.277071528089092	28.608591338572065	32.39491920019517	22.719417933143678
4	11.620213599703098	19.58877339600357	40.70623985687447	28.08477314741886
5	11.87143231813163	39.218281347473166	37.02267954766226	11.887606786732944
6	27.916175919364715	40.6347203077347	19.27653001264222	12.172573760258363
7	24.361661529489442	33.82557769964691	25.25850083048488	16.554259940378767
8	23.19113211093998	38.43176179987676	21.868365136776635	16.508740952406626
9	24.79857796376816	16.739484357529427	22.35219987692176	36.10973780178065
10-11	23.211365630344865	27.563465497193597	30.396379429574232	18.828789442887302
12-13	23.792885106432017	25.247311431881876	30.504785409970818	20.45501805171529
14-15	21.108031354186803	26.75613109592005	27.657147772393948	24.478689777499195
16-17	20.707214234849374	29.873919399906633	28.293775679669793	21.125090685574197
18-19	20.756972043916193	29.22999422317794	29.801353769362244	20.21167996354362
20-21	21.513331078152312	28.34318538745596	29.638676268764414	20.504807265627313
22-23	21.329543123303964	28.25667232889577	29.460255767140627	20.95352878065964
24-25	21.084623867814848	28.455620730139596	29.421509815586756	21.0382455864588
26-27	21.04621827406724	28.516704862381626	29.339921815244104	21.09715504830703
28-29	21.158340612028784	28.56982008095455	29.302260389210527	20.969578917806135
30-31	21.35200926968227	28.452635427328275	29.177011249887446	21.01834405310201
32-33	21.178942754135132	28.667378703851554	29.12976479282088	21.023913749192438
34-35	21.28168101108173	28.623569152321686	29.17859813237954	20.916151704217043
36-37	21.264160260422667	28.55828761548278	29.161556947742334	21.015995176352217
38-39	21.225831578947368	28.63047894736842	29.14941052631579	20.99427894736842
40-41	21.329801884915373	28.5346809591984	29.088330974839387	21.047186181046843
42-43	21.265746450504107	28.687104806825165	28.82756793225453	21.219580810416193
44-45	21.33153239892444	28.737488921259168	28.67377110239894	21.257207577417446
46-47	21.433105060782342	28.679145887649526	28.46501877023952	21.422730281328615
48-49	21.3954406843827	28.720476704889776	28.416980878276345	21.467101732451184
50-51	21.37219317592396	28.82203270259358	28.291083905781562	21.514690215700895
52-53	21.46089630781865	28.741555930885347	28.237556141526017	21.55999161976999
54-55	21.49254219528528	28.61585462775246	28.2360755415107	21.65552763545156
56-57	21.46379277519985	28.704499902484077	28.279229374945864	21.552477947370217
58-59	21.476241838673175	28.675855716129302	28.25325754108806	21.594644904109465
60-61	21.603135436520457	28.69466506115482	28.203473634287473	21.49872586803726
62-63	21.595393890942926	28.74659589097351	28.10434708824748	21.553663129836092
64-65	21.546317290477504	28.624378395149552	28.145675011380938	21.683629302992006
66-67	21.55741326332682	28.64489643780146	28.04623076270481	21.751459536166912
68-69	21.477055162149263	28.607514280579103	28.116987582165365	21.798442975106266
70-71	21.553365661197944	28.42793543942797	28.13145172780644	21.887247171567644
72-73	21.635573723286075	28.494252785480317	28.04220767539567	21.82796581583794
74-75	21.437222052614764	28.61132098706752	28.100674711004597	21.850782249313124
76-77	21.572496793006664	28.513004062604363	28.10269367357932	21.81180547080965
78-79	21.496526193908704	28.48508750938531	28.005662952176717	22.01272334452927
80-81	21.574561396222254	28.513378100517944	28.040267207594248	21.871793295665544
82-83	21.57950979053836	28.455061384184592	28.12769410088412	21.83773472439293
84-85	21.68701598542225	28.461895025931135	28.001667659884884	21.849421328761732
86-87	21.518770358900948	28.539731810280607	28.12651367972071	21.81498415109774
88-89	21.587277047708316	28.468189413978685	28.011293660364565	21.93323987794844
90-91	21.636383268767233	28.47443528076393	28.08896521989559	21.80021623057325
92-93	21.614517361624472	28.38243595230005	28.106681604920887	21.89636508115459
94-95	21.367891382574506	28.40164813338352	28.221859052447567	22.008601431594407
96-97	21.347714960002268	28.55685277503622	28.202132297649545	21.893299967311965
98-99	21.515050730311778	29.136615269092044	28.293927861156053	21.054406139440125
100-101	24.82566643108776	36.398747737573615	29.305106676273958	9.470479155064664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2611.0
1	2322.5
2	2851.5
3	3796.5
4	4810.0
5	7174.0
6	9165.5
7	10568.0
8	11369.0
9	11263.5
10	10688.5
11	9923.5
12	10849.5
13	14278.5
14	22067.5
15	32945.0
16	41727.5
17	46441.0
18	47920.0
19	47648.0
20	46850.5
21	47244.5
22	50575.5
23	58599.5
24	72668.5
25	94810.0
26	123725.5
27	155988.0
28	194485.0
29	237419.0
30	278446.5
31	317631.5
32	351817.0
33	377587.0
34	397374.5
35	412142.0
36	425326.5
37	433060.5
38	430296.0
39	418827.0
40	403485.5
41	388178.5
42	373371.0
43	361471.5
44	352089.5
45	345928.5
46	339850.0
47	332947.5
48	328279.5
49	322181.0
50	315546.0
51	309584.5
52	300637.5
53	288343.5
54	273333.5
55	254884.5
56	235377.0
57	215458.0
58	193928.5
59	169149.5
60	141423.0
61	115757.5
62	92334.0
63	71335.5
64	54151.0
65	40906.0
66	30691.5
67	22995.0
68	17406.0
69	12696.0
70	9091.0
71	6609.0
72	4675.5
73	3252.0
74	2253.5
75	1538.5
76	1050.5
77	684.0
78	462.0
79	316.0
80	219.5
81	153.0
82	111.5
83	82.0
84	60.0
85	41.0
86	26.0
87	17.0
88	10.0
89	6.5
90	5.0
91	6.0
92	6.5
93	4.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003282223590215516
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	9.774647055500754E-5
20-21	0.0021402324549685573
22-23	3.6532590003180907E-4
24-25	2.0587402866703208E-5
26-27	3.192549454650762E-4
28-29	0.0036430797947322926
30-31	3.7668907639863366E-4
32-33	4.656766553446874E-5
34-35	0.0024924663905186994
36-37	0.057802294543351694
38-39	0.03313112491086507
40-41	0.04357716393129288
42-43	9.42912387420413E-4
44-45	0.0023498371492468744
46-47	0.001572908013160691
48-49	5.43598152720351E-4
50-51	5.612328939721623E-6
52-53	0.0010100662751014236
54-55	0.002180513212475519
56-57	0.0012548808911792273
58-59	0.003849305232777317
60-61	0.0014912021755804667
62-63	1.0878390771740237E-4
64-65	7.101556171350265E-4
66-67	4.158072799042079E-4
68-69	1.8262784720749742E-4
70-71	0.001202240362125029
72-73	0.0019222604119949567
74-75	6.921958152082408E-4
76-77	0.0011848480823936664
78-79	1.4284560831720594E-4
80-81	0.002083535658951378
82-83	0.0025088226342031596
84-85	0.0038871345385315084
86-87	0.004053356781941455
88-89	0.06938739238156973
90-91	7.234031503674165E-4
92-93	7.80200691023752E-5
94-95	4.00849480218479E-5
96-97	0.008697204497302827
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	271.0
20-21	972.0
22-23	2057.0
24-25	4278.0
26-27	5949.0
28-29	10084.0
30-31	24883.0
32-33	31704.0
34-35	44129.0
36-37	70688.0
38-39	83496.0
40-41	85406.0
42-43	93946.0
44-45	109743.0
46-47	110696.0
48-49	106508.0
50-51	99300.0
52-53	95911.0
54-55	105070.0
56-57	111146.0
58-59	111772.0
60-61	111408.0
62-63	105781.0
64-65	108407.0
66-67	115869.0
68-69	120192.0
70-71	121350.0
72-73	114974.0
74-75	115361.0
76-77	115656.0
78-79	126619.0
80-81	129333.0
82-83	123940.0
84-85	123959.0
86-87	130951.0
88-89	143059.0
90-91	155520.0
92-93	165367.0
94-95	211292.0
96-97	265119.0
98-99	789528.0
100-101	5017327.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.31426617666199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41598847381307	75.39043452630416
2	7.204531734611487	12.148896127004823
3	1.8516912998826855	4.683719794059539
4	0.6867058811978948	2.315964098095941
5	0.3246396825352443	1.3685878302391816
6	0.16319817178954182	0.8255960457484824
7	0.09890957739337278	0.5837641905036187
8	0.06961610585130823	0.4695704703143902
9	0.04586392965200399	0.3480285215328089
>10	0.13661806874018131	1.7249554165625327
>50	0.0019699418565351603	0.10271248938990278
>100	2.671326765821102E-4	0.037770490244663966
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.144551081842503E-5	0.0	0.0	0.0
3	0.0	6.173461298211003E-5	0.0	0.0	0.0
4	0.0	1.0289102163685005E-4	0.0	5.144551081842503E-5	0.0
5	0.0	1.0289102163685005E-4	0.0	5.144551081842503E-5	0.0
6	0.0	1.0289102163685005E-4	0.0	5.144551081842503E-5	0.0
7	0.0	1.0289102163685005E-4	0.0	5.144551081842503E-5	0.0
8	0.0	1.2346922596422007E-4	0.0	5.144551081842503E-5	0.0
9	0.0	1.749147367826451E-4	0.0	1.4404743029159008E-4	0.0
10-11	0.0	1.749147367826451E-4	0.0	1.852038389463301E-4	0.0
12-13	0.0	1.800592878644876E-4	0.0	2.8809486058318015E-4	0.0
14-15	0.0	2.9323941166502264E-4	0.0	3.241067181560777E-4	0.0
16-17	0.0	3.1896216707423514E-4	0.0	3.3954037140160515E-4	0.0
18-19	0.0	3.4468492248344764E-4	0.0	3.498294735652902E-4	0.0
20-21	0.0	3.704076778926602E-4	0.0	3.6011857572897517E-4	0.0
22-23	0.0	4.218531887110852E-4	0.0	3.704076778926602E-4	0.0
24-25	0.0	4.7329869952951025E-4	0.0	3.909858822200302E-4	0.0
26-27	0.0	4.938769038568803E-4	0.0	4.4757594412029775E-4	0.0
28-29	0.0	4.938769038568803E-4	0.0	6.430688852303128E-4	0.0
30-31	0.0	5.453224146753053E-4	0.0	0.0017748701232356632	0.0
32-33	0.0	5.453224146753053E-4	0.0	0.004285411051174805	0.0
34-35	0.0	6.122015787392578E-4	0.0	0.007675670214109013	0.0
36-37	0.0	6.945143960487379E-4	0.0	0.012897389562179153	0.0
38-39	0.0	7.202371514579503E-4	0.0	0.021365320642891913	0.0
40-41	0.0	7.408153557853204E-4	0.0	0.031073088534328715	0.0
42-43	0.0	7.922608666037454E-4	0.0	0.04047732791193681	0.0
44-45	1.0289102163685005E-5	9.002964393224379E-4	0.0	0.05085388744401314	0.0
46-47	1.0289102163685005E-5	9.671756033863905E-4	0.0	0.06367410873996465	0.0
48-49	1.0289102163685005E-5	9.82609256631918E-4	0.0	0.07580496019094927	0.0
50-51	1.0289102163685005E-5	0.0011112230336779805	0.0	0.08780719786488783	0.0
52-53	1.0289102163685005E-5	0.0011215121358416655	0.0	0.09937729324795161	0.0
54-55	1.0289102163685005E-5	0.0011318012380053507	0.0	0.11177051680411021	0.0
56-57	1.0289102163685005E-5	0.0011626685444964056	0.0	0.12434894419921513	0.0
58-59	1.0289102163685005E-5	0.0011678130955782482	0.0	0.13537886171868546	0.0
60-61	1.0289102163685005E-5	0.001208969504232988	0.0	0.14601264880485393	0.0
62-63	1.0289102163685005E-5	0.0012346922596422007	0.0	0.15497445678942356	0.0
64-65	1.0289102163685005E-5	0.0013067159747879957	0.0	0.16505777690983484	0.0
66-67	1.0289102163685005E-5	0.0013118605258698383	0.0	0.17562468483193935	0.0
68-69	1.0289102163685005E-5	0.0013324387301972083	0.0	0.18650541037003623	0.0
70-71	1.0289102163685005E-5	0.0013478723834427356	0.0	0.19728838943757815	0.0
72-73	1.0289102163685005E-5	0.0013478723834427356	0.0	0.2065228586294854	0.0
74-75	1.0289102163685005E-5	0.0013890287920974758	0.0	0.21493934419937977	0.0
76-77	1.0289102163685005E-5	0.001455907956161428	0.0	0.22264588171997982	0.0
78-79	1.0289102163685005E-5	0.0014713416094069556	0.0	0.229663049395613	0.0
80-81	1.0289102163685005E-5	0.0014713416094069556	0.0	0.23406678512167017	0.0
82-83	1.0289102163685005E-5	0.0014713416094069556	0.0	0.23788918657547914	0.0
84-85	1.0289102163685005E-5	0.0014816307115706408	0.0	0.2415366732925055	0.0
86-87	1.0289102163685005E-5	0.0014970643648161684	0.0	0.24212829666691738	0.0
88-89	1.0289102163685005E-5	0.0015433653245527507	0.0	0.24220546493314501	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10930	0.0	40.93173	1
GTATCAA	18795	0.0	32.587902	1
TCAACGC	22605	0.0	26.332756	4
ATCAACG	22890	0.0	26.004892	3
CAACGCA	23035	0.0	25.787842	5
AACGCAG	23230	0.0	25.606642	6
TATCAAC	24420	0.0	24.6285	2
ACGCAGA	27225	0.0	21.819025	7
CGCAGAG	28060	0.0	21.213541	8
GTACATG	28005	0.0	20.290768	1
ACATGGG	27715	0.0	19.762949	3
TACATGG	28560	0.0	19.695631	2
GCAGAGT	33230	0.0	17.802134	9
AGAGTAC	30030	0.0	17.789244	10-11
CATGGGG	19790	0.0	17.181755	4
GAGTACT	17315	0.0	16.739372	12-13
CAGAGTA	32765	0.0	15.785432	10-11
TATACCG	735	0.0	15.049139	5
AGTACTT	20970	0.0	14.632503	12-13
ATGGGGG	11515	0.0	14.479904	5
>>END_MODULE