FastQCFastQC Report
Fri 27 May 2016
SRR2139189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17591759
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207800.043.1838341
GTATCAA354300.030.9201181
TCAACGC411850.025.644784
ATCAACG415300.025.4514313
CAACGCA419950.025.1306695
AACGCAG423550.024.9556836
TATCAAC448600.023.8089922
ACGCAGA492050.021.4483997
CGCAGAG504000.020.9560768
GTACATG469450.019.2633841
TACATGG473250.018.4426272
ACATGGG469400.018.1364083
GCAGAGT592900.017.7316469
AGAGTAC539500.017.03104610-11
GAGTACT345000.016.72987612-13
CATGGGG327600.016.450734
CAGAGTA585550.016.38999410-11
AGTACTT390750.014.50424612-13
GTACTTT402000.014.35394814-15
GTGGTAT116700.013.7863051
Produced by FastQC (version 0.11.5)