##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139189_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17591759
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.558545055102222	31.0	31.0	34.0	30.0	34.0
2	31.67074554625265	31.0	31.0	34.0	30.0	34.0
3	31.735292815232405	31.0	31.0	34.0	30.0	34.0
4	35.51601502726362	37.0	35.0	37.0	33.0	37.0
5	35.35504317675112	37.0	35.0	37.0	33.0	37.0
6	35.37987429227515	37.0	35.0	37.0	33.0	37.0
7	35.28225056971279	37.0	35.0	37.0	32.0	37.0
8	35.26347604011628	37.0	35.0	37.0	32.0	37.0
9	36.843923566710984	39.0	37.0	39.0	32.0	39.0
10-11	36.714533947401165	39.0	35.0	39.0	32.0	39.0
12-13	36.68031582288047	39.0	35.0	39.0	32.0	39.0
14-15	37.80354986104573	40.0	37.0	41.0	32.0	41.0
16-17	37.763144407560375	40.0	37.0	41.0	32.0	41.0
18-19	37.79134161626475	40.0	37.0	41.0	32.0	41.0
20-21	37.77008171771202	40.0	37.0	41.0	32.0	41.0
22-23	37.676118374206304	39.5	36.5	41.0	32.0	41.0
24-25	37.6104475767355	39.0	36.0	41.0	32.0	41.0
26-27	37.41502399785656	39.0	36.0	41.0	31.5	41.0
28-29	37.26503574405422	39.0	36.0	41.0	31.0	41.0
30-31	37.116937459642266	39.0	36.0	40.5	31.0	41.0
32-33	36.90470834419442	39.0	35.5	40.0	30.0	41.0
34-35	36.782682774380156	39.0	35.0	40.0	30.0	41.0
36-37	36.63697954859852	38.0	35.0	40.0	30.0	41.0
38-39	36.52106834124899	38.0	35.0	40.0	30.0	41.0
40-41	36.39795697161951	38.0	35.0	40.0	30.0	41.0
42-43	36.16389585272537	38.0	35.0	40.0	29.5	41.0
44-45	36.007767569488756	38.0	34.0	40.0	29.0	41.0
46-47	36.01912743516117	38.0	34.5	40.0	29.0	41.0
48-49	36.311736992282334	38.0	35.0	40.0	29.5	41.0
50-51	36.61626334998233	39.0	35.0	40.0	30.0	41.0
52-53	36.60152070831006	39.0	35.0	40.0	30.0	41.0
54-55	36.51759004005663	38.5	35.0	40.0	30.0	41.0
56-57	36.389342832531824	38.0	35.0	40.0	29.5	41.0
58-59	36.243093446037804	38.0	34.0	40.0	29.0	41.0
60-61	36.06398568276141	38.0	34.0	40.0	29.0	41.0
62-63	35.86728117792407	38.0	34.0	40.0	28.0	41.0
64-65	35.62540544467684	37.0	34.0	40.0	28.0	41.0
66-67	35.390250396919654	37.0	34.0	40.0	28.0	41.0
68-69	35.12218370713481	36.5	33.0	39.0	28.0	41.0
70-71	34.839062088526354	36.0	33.0	39.0	28.0	41.0
72-73	34.56372243185576	35.5	33.0	39.0	28.0	40.0
74-75	34.25232973666181	35.0	32.5	38.0	27.5	40.0
76-77	32.5150123465991	34.0	30.5	36.0	25.5	39.0
78-79	33.52449630411472	35.0	32.0	37.0	26.5	39.0
80-81	33.566506541557686	35.0	32.0	36.5	27.0	39.0
82-83	33.35249782850083	35.0	32.0	36.0	27.0	38.5
84-85	33.112603980362216	35.0	32.0	36.0	27.0	37.0
86-87	32.8998441853857	34.5	32.0	35.0	27.0	37.0
88-89	32.739335863808705	34.0	32.0	35.0	27.0	36.5
90-91	32.616023917665814	34.0	32.0	35.0	27.0	36.0
92-93	32.543231511926294	34.0	32.0	35.0	27.0	36.0
94-95	32.51995472147986	34.0	32.0	35.0	27.0	35.5
96-97	32.60382838071526	34.0	32.0	35.0	27.5	35.0
98-99	32.73086005445067	34.0	32.0	35.0	29.0	35.0
100	31.500337391849133	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	34.0
20	216.0
21	1548.0
22	6584.0
23	22460.0
24	55786.0
25	111903.0
26	189707.0
27	284675.0
28	388373.0
29	495634.0
30	606691.0
31	725279.0
32	860769.0
33	1036173.0
34	1278938.0
35	1612221.0
36	2155405.0
37	2932327.0
38	3152232.0
39	1615627.0
40	59173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.4572651887739	22.46483140202182	15.079731367397656	22.998172041806622
2	14.162201449606412	22.676377069754583	41.96695130469244	21.194470175946567
3	17.396031857871634	28.291235685982286	31.82143411582662	22.491298340319467
4	12.198404946316057	19.26373025005629	40.5109801697488	28.02688463387885
5	12.010697736366216	39.115258457099145	36.92900749720366	11.945036309330977
6	27.967345391668907	40.45160577745523	19.32920977373553	12.251839057140334
7	24.388760804655735	33.762385512913404	25.28161825203923	16.567235430391637
8	22.982897844382702	38.544053496867484	21.977347461388028	16.495701197361786
9	24.7160957582468	17.005320502628532	22.460505512837006	35.81807822628766
10-11	23.201577443972315	27.549661062714055	30.4045788508818	18.84418264243182
12-13	23.863479426707453	25.245215065708493	30.467838861866596	20.423466645717454
14-15	21.140706684072708	26.730722825303786	27.682683122252417	24.44588736837109
16-17	20.69477150792367	29.87613040357723	28.356006681777558	21.07309140672155
18-19	20.726352047508648	29.16789096249406	29.902937428890652	20.202819561106644
20-21	21.443892744252636	28.322719456686247	29.754062865208052	20.479324933853064
22-23	21.330905335223083	28.212019898364655	29.532392410624876	20.92468235578739
24-25	21.090827852257917	28.405839708488916	29.500328625648635	21.003003813604533
26-27	21.05926720446268	28.47220048651809	29.439992592851418	21.02853971616781
28-29	21.159374665907457	28.456642114288254	29.438088882157754	20.945894337646532
30-31	21.352123355188123	28.375605899233246	29.262797837719646	21.00947290785898
32-33	21.171581190262927	28.626079590719257	29.205933673131586	20.996405545886233
34-35	21.317889517316186	28.53260738370525	29.24626390252661	20.90323919645195
36-37	21.300246050861567	28.511763717204786	29.144488634930436	21.043501597003207
38-39	21.21191503544324	28.59657766458828	29.21340296938722	20.97810433058126
40-41	21.338198913878514	28.475478480193484	29.10923876960973	21.077083836318273
42-43	21.337726443382525	28.636416848563073	28.835860431183143	21.189996276871256
44-45	21.348800386234675	28.708191470696864	28.682523027782743	21.26048511528572
46-47	21.441997765809976	28.653664588998396	28.49495001140156	21.409387633790068
48-49	21.40387910124227	28.72755101414211	28.430652698545263	21.43791718607036
50-51	21.38125932104223	28.84634191062218	28.301119732739927	21.471279035595664
52-53	21.476588974639117	28.749842677332072	28.278345464571874	21.49522288345694
54-55	21.48018277277294	28.66578445904707	28.287680067369735	21.56635270081025
56-57	21.454956821554756	28.73165135213947	28.30418220771542	21.509209618590347
58-59	21.486809673727336	28.710467040910398	28.2901369184859	21.51258636687637
60-61	21.546866924244732	28.731582277899665	28.243346775209098	21.478204022646512
62-63	21.577291407022827	28.83921986779215	28.14948005008629	21.434008675098738
64-65	21.515459836619076	28.68739789535778	28.17235347973296	21.62478878829019
66-67	21.509253387115674	28.658317247762717	28.13614948689432	21.696279878227298
68-69	21.46604375262385	28.67009576102101	28.17261925212941	21.691241234225732
70-71	21.529580532736748	28.512339602195574	28.224413909521452	21.733665955546225
72-73	21.615289262702372	28.541089163631234	28.1153809350912	21.72824063857519
74-75	21.458801942857214	28.691305780230852	28.13905636218681	21.710835914725127
76-77	21.56423573584811	28.601974580394042	28.176994228942675	21.65679545481517
78-79	21.499696489978312	28.546134959434326	28.101420877513068	21.85274767307429
80-81	21.553264076323458	28.61520332719994	28.123227397286865	21.708305199189738
82-83	21.59016886068865	28.561564794506637	28.195560058736902	21.652706286067808
84-85	21.65366312256475	28.54435611264854	28.126796454575477	21.675184310211236
86-87	21.492361922457267	28.605772126531576	28.25936715066221	21.642498800348946
88-89	21.581112553265232	28.56623860483294	28.148871001409137	21.703777840492698
90-91	21.586252263994325	28.633029934469977	28.169902311993887	21.610815489541807
92-93	21.647684904878748	28.486349489606237	28.196586044634227	21.669379560880788
94-95	21.419804182633264	28.509261280807745	28.336981719326115	21.733952817232876
96-97	21.441017361312255	28.656346843309176	28.403834369290642	21.498801426087926
98-99	22.484595299109884	30.57788158419378	28.65778912041465	18.27973399628169
100	21.781371152338497	31.253865446439054	22.469462103288905	24.495301297933544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4803.0
1	4094.0
2	5123.5
3	7045.5
4	8790.5
5	13949.5
6	18798.0
7	22165.5
8	24244.0
9	23654.5
10	21249.5
11	18565.5
12	19482.5
13	24958.0
14	38733.5
15	58725.0
16	75469.0
17	85362.5
18	89457.0
19	90298.0
20	89051.5
21	87169.0
22	88681.0
23	98581.0
24	119673.0
25	153321.5
26	198770.5
27	252942.0
28	319308.0
29	396712.5
30	470609.5
31	540952.5
32	605862.5
33	658261.0
34	703761.5
35	742566.5
36	777454.5
37	801798.0
38	803143.5
39	786197.5
40	764340.0
41	738300.0
42	709778.5
43	687008.5
44	665300.5
45	648937.5
46	632798.5
47	613820.0
48	601090.5
49	586978.0
50	573633.5
51	559981.0
52	540877.5
53	516020.0
54	485336.0
55	453077.0
56	420535.5
57	384950.0
58	345758.5
59	303002.5
60	254769.0
61	208516.5
62	168190.0
63	131323.5
64	99522.5
65	75494.0
66	57480.5
67	44126.0
68	33779.0
69	24898.5
70	18125.5
71	13149.5
72	9452.5
73	6635.0
74	4514.5
75	3119.0
76	2200.5
77	1533.5
78	1100.5
79	799.5
80	561.5
81	387.0
82	288.5
83	197.5
84	126.5
85	81.0
86	56.5
87	44.5
88	35.5
89	30.0
90	23.0
91	19.5
92	15.0
93	8.5
94	3.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.10951150479039647
3	0.0
4	0.0
5	0.0
6	0.0
7	2.1032575537216033E-4
8	0.0
9	0.0
10-11	5.116031887430927E-5
12-13	4.263359906192439E-5
14-15	9.322546994874134E-4
16-17	1.8758783587246732E-4
18-19	7.958271824892553E-5
20-21	7.304995974861945E-4
22-23	3.6957136970398896E-4
24-25	3.3271956526690976E-4
26-27	0.002008812941216841
28-29	0.004066614795495125
30-31	0.0111226842052293
32-33	0.008277628452578935
34-35	0.007379344272847232
36-37	0.003796500083427656
38-39	7.336554022322514E-4
40-41	1.9676394382371783E-4
42-43	3.6472058385419927E-4
44-45	2.9975984140785927E-5
46-47	0.0
48-49	2.149256202300521E-5
50-51	0.0
52-53	0.0013621368147792724
54-55	6.631585052902279E-4
56-57	7.930599866399896E-4
58-59	4.551312143290911E-5
60-61	4.6091110075085383E-4
62-63	3.933392858678595E-4
64-65	8.335347709029682E-4
66-67	8.88933776245727E-4
68-69	3.328286703054317E-4
70-71	2.532507191968688E-4
72-73	3.56910202677883E-6
74-75	3.6207614461321213E-6
76-77	3.453708928833278E-4
78-79	1.3055781835050566E-4
80-81	3.147070848641021E-4
82-83	7.326490391307851E-5
84-85	3.920783240753185E-5
86-87	0.0
88-89	1.2192559035659618E-5
90-91	0.0
92-93	0.0
94-95	1.1780232691009602E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	632.0
20-21	2229.0
22-23	4618.0
24-25	8811.0
26-27	13181.0
28-29	22107.0
30-31	56487.0
32-33	64448.0
34-35	85689.0
36-37	121984.0
38-39	148349.0
40-41	158443.0
42-43	176526.0
44-45	198223.0
46-47	198890.0
48-49	181860.0
50-51	175668.0
52-53	171880.0
54-55	181654.0
56-57	192118.0
58-59	192362.0
60-61	190611.0
62-63	182394.0
64-65	188273.0
66-67	201259.0
68-69	206010.0
70-71	207686.0
72-73	200392.0
74-75	203020.0
76-77	198210.0
78-79	216031.0
80-81	221133.0
82-83	214579.0
84-85	215577.0
86-87	227261.0
88-89	249329.0
90-91	273825.0
92-93	297254.0
94-95	355896.0
96-97	729813.0
98-99	1116276.0
100-101	9340771.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.28658854700403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.4956723972613	72.82006982743901
2	7.549534466870328	12.424508727935681
3	2.0325609403256686	5.017575173798697
4	0.8151393758303227	2.683001537096458
5	0.410906958359733	1.690606590682413
6	0.23150885602812532	1.1430044388584364
7	0.13692296746957727	0.7886846720762746
8	0.0865729704693612	0.5699035520243404
9	0.05536096812362763	0.4099918684997477
>10	0.1830740192628196	2.2556071785116267
>50	0.002372262967925122	0.1303368300242972
>100	3.347370395836408E-4	0.04492849535447683
>500	3.9079991592558284E-5	0.021781107698534294
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.684479874923252E-6
2	0.0	5.684479874923252E-6	0.0	0.0	5.684479874923252E-6
3	0.0	5.684479874923252E-6	0.0	5.684479874923252E-6	5.684479874923252E-6
4	0.0	1.1368959749846505E-5	0.0	1.1368959749846505E-5	5.684479874923252E-6
5	0.0	1.1368959749846505E-5	0.0	1.1368959749846505E-5	5.684479874923252E-6
6	9.095167799877204E-5	1.1368959749846505E-5	0.0	1.1368959749846505E-5	1.1368959749846505E-5
7	9.095167799877204E-5	1.1368959749846505E-5	0.0	2.273791949969301E-5	1.1368959749846505E-5
8	1.0232063774861855E-4	1.1368959749846505E-5	0.0	2.273791949969301E-5	1.1368959749846505E-5
9	1.0232063774861855E-4	6.821375849907904E-5	0.0	5.684479874923253E-5	2.273791949969301E-5
10-11	1.0232063774861855E-4	6.821375849907904E-5	0.0	6.252927862415578E-5	2.273791949969301E-5
12-13	1.0232063774861855E-4	6.821375849907904E-5	0.0	9.095167799877204E-5	2.273791949969301E-5
14-15	1.080051176235418E-4	1.1368959749846505E-4	0.0	9.095167799877204E-5	2.5580159437154636E-5
16-17	1.8190335599754408E-4	1.1368959749846505E-4	0.0	9.095167799877204E-5	2.8422399374616264E-5
18-19	1.847455959350057E-4	1.279007971857732E-4	0.0	9.663615787369529E-5	2.8422399374616264E-5
20-21	1.9043007580992898E-4	1.4779647674800455E-4	0.0	1.0516287768608018E-4	2.8422399374616264E-5
22-23	1.9327231574739058E-4	1.847455959350057E-4	0.0	1.3926975693561968E-4	3.126463931207789E-5
24-25	1.9327231574739058E-4	2.1032575537216033E-4	0.0	1.847455959350057E-4	3.410687924953952E-5
26-27	1.9327231574739058E-4	2.2169471512200684E-4	0.0	2.9275071355854747E-4	3.410687924953952E-5
28-29	1.9327231574739058E-4	2.2169471512200684E-4	0.0	9.237279796750284E-4	3.410687924953952E-5
30-31	1.9895679562231382E-4	2.387481547467766E-4	0.0	0.003416372404828875	3.410687924953952E-5
32-33	2.046412754972371E-4	2.4159039468423822E-4	0.0	0.009063903160565125	3.410687924953952E-5
34-35	2.0748351543469873E-4	2.9275071355854747E-4	0.0	0.016081393566157884	4.547583899938602E-5
36-37	2.1316799530962197E-4	3.4391103243285677E-4	0.0	0.02666021061339005	4.547583899938602E-5
38-39	2.160102352470836E-4	3.666489519325498E-4	0.0	0.04028022439370617	4.547583899938602E-5
40-41	2.2169471512200684E-4	4.1496703086939745E-4	0.0	0.05552884165818779	4.547583899938602E-5
42-43	2.2169471512200684E-4	4.234937506817823E-4	0.0	0.06526635568393133	4.8318078936847646E-5
44-45	2.3022143493439171E-4	4.6896958968116833E-4	0.0	0.08048086606916341	5.40025588117709E-5
46-47	2.387481547467766E-4	5.059187088681695E-4	0.0	0.09350969394248751	6.537151856161741E-5
48-49	2.387481547467766E-4	5.087609488056311E-4	0.0	0.10640777877868836	7.389823837400228E-5
50-51	2.4727487455916144E-4	5.684479874923252E-4	0.0	0.1204996043886231	7.389823837400228E-5
52-53	2.5580159437154634E-4	5.684479874923252E-4	0.0	0.13143370142803798	8.526719812384878E-5
54-55	2.5580159437154634E-4	5.684479874923252E-4	0.0	0.14481212481366984	9.095167799877204E-5
56-57	2.614860742464696E-4	5.798169472421718E-4	0.0	0.15799443364361687	9.095167799877204E-5
58-59	2.614860742464696E-4	5.85501427117095E-4	0.0	0.16903937804059277	9.379391793623367E-5
60-61	2.728550339963161E-4	5.911859069920183E-4	0.0	0.17883089462514806	9.947839781115691E-5
62-63	2.955929534960091E-4	6.025548667418647E-4	0.0	0.18724108260009703	1.0232063774861855E-4
64-65	3.0980415318331727E-4	6.76453105115867E-4	0.0	0.1947389115551208	1.0232063774861855E-4
66-67	3.126463931207789E-4	6.76453105115867E-4	0.0	0.2022338982702071	1.0232063774861855E-4
68-69	3.126463931207789E-4	6.906643048031752E-4	0.0	0.2097004625859188	1.0232063774861855E-4
70-71	3.353843126204719E-4	7.048755044904833E-4	0.0	0.2161949808430186	1.2505855724831156E-4
72-73	3.751756717449347E-4	7.048755044904833E-4	0.0	0.22132238169019938	1.2505855724831156E-4
74-75	3.751756717449347E-4	7.190867041777915E-4	0.0	0.2253867848007695	1.2505855724831156E-4
76-77	3.808601516198579E-4	7.446668636149461E-4	0.0	0.22906180103990736	1.279007971857732E-4
78-79	3.808601516198579E-4	7.53193583427331E-4	0.0	0.2314578093071875	1.307430371232348E-4
80-81	3.808601516198579E-4	7.588780633022542E-4	0.0	0.2322678476893641	1.307430371232348E-4
82-83	3.808601516198579E-4	7.617203032397158E-4	0.0	0.23283629567685643	1.307430371232348E-4
84-85	3.8654463149478117E-4	7.702470230521008E-4	0.0	0.23308641279135303	1.307430371232348E-4
86-87	3.922291113697044E-4	7.730892629895623E-4	0.0	0.2332228403083512	1.307430371232348E-4
88	4.092825509944742E-4	8.128806221140251E-4	0.0	0.23322852478822612	1.307430371232348E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20780	0.0	43.183834	1
GTATCAA	35430	0.0	30.920118	1
TCAACGC	41185	0.0	25.64478	4
ATCAACG	41530	0.0	25.451431	3
CAACGCA	41995	0.0	25.130669	5
AACGCAG	42355	0.0	24.955683	6
TATCAAC	44860	0.0	23.808992	2
ACGCAGA	49205	0.0	21.448399	7
CGCAGAG	50400	0.0	20.956076	8
GTACATG	46945	0.0	19.263384	1
TACATGG	47325	0.0	18.442627	2
ACATGGG	46940	0.0	18.136408	3
GCAGAGT	59290	0.0	17.731646	9
AGAGTAC	53950	0.0	17.031046	10-11
GAGTACT	34500	0.0	16.729876	12-13
CATGGGG	32760	0.0	16.45073	4
CAGAGTA	58555	0.0	16.389994	10-11
AGTACTT	39075	0.0	14.504246	12-13
GTACTTT	40200	0.0	14.353948	14-15
GTGGTAT	11670	0.0	13.786305	1
>>END_MODULE