FastQCFastQC Report
Fri 27 May 2016
SRR2139188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25757582
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA293600.048.357411
GTATCAA487300.030.6662881
ATCAACG666000.026.2188762
TCAACGC674500.025.858773
TATCAAC698450.025.2780741
CAACGCA689550.025.1811734
AACGCAG713950.024.4118045
ACGCAGA824700.020.9489656
CGCAGAG842300.020.6980927
CAGAGTA903200.018.4535669
GCAGAGT911600.018.0488598
AGAGTAC836650.017.67696610-11
TATACCG23050.015.9314375
GAGTACT621350.015.62395112-13
AGTACTT686600.015.61161412-13
GTACATG543600.014.6169731
TACATGG545550.014.2474442
GTACTTT702650.013.87350514-15
GTGGTAT136050.013.843551
TATACTG161700.013.4762235
Produced by FastQC (version 0.11.5)