##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139188_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25757582
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.269155777122247	31.0	30.0	31.0	26.0	31.0
2	29.569962040691554	31.0	30.0	31.0	26.0	31.0
3	28.95050517552463	31.0	28.0	31.0	25.0	33.0
4	32.582009600124735	35.0	32.0	35.0	26.0	37.0
5	32.21733177438783	35.0	32.0	35.0	25.0	37.0
6	31.60961498637566	35.0	30.0	35.0	23.0	37.0
7	32.05316923770251	35.0	31.0	35.0	25.0	37.0
8	31.620563568428125	35.0	30.0	35.0	23.0	37.0
9	32.82064644111392	35.0	30.0	37.0	25.0	39.0
10-11	32.841515306056294	35.0	30.5	37.0	25.0	39.0
12-13	32.82376637682839	35.0	30.5	37.0	24.0	39.0
14-15	33.58839653504743	36.0	31.5	38.0	25.0	40.0
16-17	33.54588483887967	36.0	31.0	38.0	25.0	40.0
18-19	33.68676623061901	36.0	31.5	38.5	25.0	40.0
20-21	33.77682953858802	36.0	31.5	39.0	25.0	40.0
22-23	33.705218608049016	36.0	31.5	39.0	25.0	40.0
24-25	33.590010213800156	36.0	31.0	38.5	25.0	40.0
26-27	33.17585264692188	36.0	31.0	38.0	24.0	40.0
28-29	32.938307034178344	36.0	30.0	38.0	23.0	40.0
30-31	32.769019992002725	35.0	30.0	38.0	23.0	40.0
32-33	32.51459258421144	35.0	30.0	38.0	21.0	40.0
34-35	32.40335962280497	35.0	30.0	38.0	21.0	40.0
36-37	32.290050496844245	35.0	30.0	38.0	21.0	40.0
38-39	32.05343963892092	35.0	30.0	38.0	19.0	40.0
40-41	31.933421535157553	35.0	30.0	38.0	19.0	40.0
42-43	31.888899852610237	34.0	30.0	38.0	19.0	40.0
44-45	31.778051139217794	34.0	29.0	38.0	19.0	40.0
46-47	31.77068620892103	34.0	29.0	38.0	19.0	40.0
48-49	31.88865591156619	34.0	29.0	38.0	20.5	40.0
50-51	31.75063889293277	34.0	29.0	38.0	19.5	40.0
52-53	31.647170158609832	34.0	28.5	38.0	19.5	40.0
54-55	31.917627597227543	34.0	29.0	38.0	21.0	40.0
56-57	31.879121621366437	34.0	28.5	38.0	21.0	40.0
58-59	31.811960494969064	34.0	28.0	38.0	21.5	40.0
60-61	31.672011608061148	34.0	28.0	38.0	21.0	40.0
62-63	31.546467312158157	34.0	28.0	37.0	21.5	39.5
64-65	31.233765795104176	33.5	28.0	37.0	20.0	39.0
66-67	31.07326105151187	33.0	28.0	37.0	20.0	39.0
68-69	30.94113460256183	33.0	28.0	36.5	20.0	39.0
70-71	30.77415151786841	32.5	28.0	36.0	20.0	39.0
72-73	30.551806301425387	32.0	28.0	36.0	20.0	39.0
74-75	30.281458429958377	32.0	27.5	35.5	20.0	38.0
76-77	28.845347800380864	30.5	26.0	34.0	19.5	36.0
78-79	29.990879060841237	31.5	27.5	35.0	20.5	37.0
80-81	30.255155770969132	32.0	28.0	35.0	21.0	37.0
82-83	30.19332442970046	32.0	28.0	34.5	21.0	36.0
84-85	30.14289320301046	32.0	28.0	34.0	21.0	36.0
86-87	30.028336538335132	32.0	28.0	34.0	21.0	36.0
88-89	29.865099898566292	32.0	27.5	34.0	20.5	35.0
90-91	29.842645282596813	32.0	28.0	34.0	21.0	35.0
92-93	29.899102809620874	32.0	28.5	34.0	21.5	35.0
94-95	30.088774783486443	32.0	29.0	34.0	23.0	35.0
96-97	30.40454601973215	32.0	29.0	34.0	24.0	35.0
98-99	30.791643417320294	32.0	29.0	34.0	25.0	35.0
100	29.96183794409614	31.0	27.0	34.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	10.0
18	139.0
19	1548.0
20	9333.0
21	42678.0
22	134177.0
23	321632.0
24	613349.0
25	979969.0
26	1355330.0
27	1687895.0
28	1944327.0
29	2107964.0
30	2206422.0
31	2260034.0
32	2274003.0
33	2249561.0
34	2162084.0
35	1987705.0
36	1670104.0
37	1149735.0
38	516126.0
39	83088.0
40	367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.3231092111053	26.086027795621497	17.279222871153046	22.311640122120156
2	15.559382650059868	26.016696148559376	37.86212152417146	20.561799677209297
3	17.815261116600954	29.257157663708956	32.64790875643472	20.279672463255373
4	13.547888478359981	22.057505701612797	38.18008880122727	26.21451701879995
5	11.612922812899239	39.902640702168426	36.849954970538576	11.634481514393762
6	26.99157087027812	41.84442080005802	19.190528055001437	11.973480274662428
7	23.418366677431134	34.24897569966001	25.311669395054242	17.020988227854616
8	22.837574582893687	38.8831257530307	21.93330491969316	16.34599474438245
9	24.12023296369102	16.43372317552719	22.333953469726048	37.112090391055744
10-11	22.87804801508432	28.215008888646796	30.235713542891695	18.67122955337718
12-13	23.57638899673561	25.8346579067521	30.207743843160156	20.381209253352132
14-15	20.789531653873926	27.19480491446904	27.699613713620703	24.316049718036332
16-17	20.31831404154677	30.16040675141062	28.41317927339403	21.10809993364858
18-19	20.380854911103384	29.392909319480914	30.08201558037341	20.14422018904229
20-21	21.157438287854777	28.541275463535715	29.923276170461417	20.37801007814809
22-23	20.910612683599755	28.54008495965603	29.79593087062976	20.753371486114457
24-25	20.899181335666974	28.61969787662521	29.798654162370745	20.682466625337067
26-27	20.975324290875797	28.679640213555164	29.646575355614107	20.69846013995493
28-29	21.031511878285198	28.67308907379237	29.64308922436948	20.652309823552958
30-31	21.197772123710383	28.574386342441354	29.570221553720643	20.657619980127624
32-33	21.154926739474902	28.71452432534294	29.419662429244852	20.71088650593731
34-35	21.210399746213728	28.697765322308605	29.49513376532753	20.596701166150137
36-37	21.194600612200908	28.713976358850168	29.324862027762844	20.766561001186084
38-39	21.15585382339338	28.739529078270515	29.317479420224103	20.787137678112007
40-41	21.283150191833304	28.658320854627828	29.231709512884123	20.826819440654738
42-43	21.180109979226444	28.82379499269692	28.99861038334374	20.997484644732896
44-45	21.20593209017789	28.929873543823355	28.803668606109344	21.06052575988941
46-47	21.271156280385302	28.87604958949511	28.68947505813278	21.163319071986813
48-49	21.223355380586995	29.00577631984565	28.613607854758538	21.157260444808816
50-51	21.190778983984814	29.05564429554109	28.499471000681027	21.25410571979307
52-53	21.16025537935061	29.035658200748536	28.501106682250484	21.30297973765037
54-55	21.160783848346437	28.9494282411581	28.608290438383303	21.28149747211216
56-57	21.161349061970252	28.88786732204109	28.59910224356162	21.351681372427038
58-59	21.087086951640284	28.962777912600746	28.568895072755918	21.381240063003055
60-61	21.167231877226666	29.01248796286672	28.571774767125458	21.248505392781155
62-63	21.100808867299982	29.11611075922981	28.555197292906207	21.227883080564002
64-65	21.045625248092673	29.144707570809388	28.431027123632408	21.37864005746553
66-67	21.061999381726	29.225845125520916	28.25774835855317	21.45440713419991
68-69	21.054563946131	29.237381023086893	28.29151358251903	21.416541448263075
70-71	21.093478578637903	29.043561743400133	28.348483189533937	21.514476488428034
72-73	21.167421961832687	29.02079845620684	28.34202800258721	21.469751579373263
74-75	21.16920367590796	29.16624519786824	28.229175582352788	21.435375543871018
76-77	21.18246330205988	29.062864156541995	28.430084024006153	21.32458851739197
78-79	21.125011738245387	29.018908561735014	28.417497337065313	21.438582362954286
80-81	21.120380886567823	29.039552414185366	28.417092488134532	21.422974211112276
82-83	21.198800282714352	29.044003415248802	28.53811709204443	21.219079209992415
84-85	21.253387837505993	29.145165812339386	28.497425212722987	21.10402113743163
86-87	21.09127547570184	29.187661531459707	28.601665739902344	21.11939725293611
88-89	21.141635015843825	29.113309185567665	28.63998185926106	21.10507393932745
90-91	21.16642542584484	29.16858786482222	28.779785116447083	20.885201592885856
92-93	21.137759396232433	29.06765599173316	28.831949861201245	20.962634750833164
94-95	20.7537564220324	29.12124224870154	29.107189767834836	21.01781156143123
96-97	20.720824578155607	29.321111844530602	29.27296560196569	20.685097975348107
98-99	21.74046316236652	31.312716770987443	29.514153954413757	17.432666112232276
100	21.370158752596215	32.07881533550044	23.359817978967563	23.191207932935786
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6657.0
1	6316.0
2	9649.5
3	13742.5
4	16244.5
5	22850.5
6	30005.5
7	37222.0
8	43866.5
9	46387.0
10	45100.5
11	42474.0
12	44989.5
13	56279.0
14	84464.5
15	126321.0
16	160448.0
17	175880.0
18	181720.5
19	187533.0
20	191609.0
21	195397.5
22	203080.5
23	222508.0
24	258250.0
25	308892.0
26	370473.0
27	433982.5
28	497244.5
29	563385.5
30	626041.5
31	688322.0
32	752420.5
33	805193.5
34	855445.0
35	908468.5
36	960017.0
37	1005229.0
38	1035044.5
39	1053286.5
40	1069190.5
41	1078284.0
42	1078628.5
43	1078633.0
44	1074624.0
45	1069753.0
46	1060837.5
47	1044760.5
48	1021112.0
49	984066.5
50	946707.5
51	908261.0
52	859789.0
53	802068.0
54	737239.0
55	671092.0
56	607048.0
57	544558.0
58	480671.0
59	414975.0
60	348992.5
61	287365.5
62	234878.0
63	188886.5
64	147723.5
65	114744.5
66	88658.5
67	69427.5
68	55188.0
69	42900.5
70	33260.5
71	25760.5
72	19680.5
73	14832.5
74	11121.0
75	8482.5
76	6516.5
77	4933.5
78	3732.5
79	2830.5
80	2125.0
81	1595.5
82	1249.0
83	879.5
84	602.5
85	448.0
86	349.5
87	280.0
88	198.0
89	129.5
90	85.5
91	61.0
92	45.0
93	21.0
94	5.5
95	3.5
96	3.0
97	2.5
98	1.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0349761091704959
3	1.8305406151866273
4	0.8186443898344186
5	3.882352000277045E-6
6	0.0
7	0.0
8	0.0
9	0.05768398602011633
10-11	0.2861099306604168
12-13	0.0033465874242388123
14-15	3.105881600221636E-5
16-17	1.222940880087269E-4
18-19	0.12203591160070848
20-21	0.0
22-23	0.0013081071192398532
24-25	1.1483674827329324E-4
26-27	0.0030432709825633448
28-29	6.257710917613129E-4
30-31	8.120074642707398E-4
32-33	0.0026638514763998116
34-35	1.2461431621879656E-4
36-37	0.0
38-39	0.0
40-41	1.8156954525140766E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0063963379766433315
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	4.604166987519554E-6
66-67	0.0
68-69	8.5649171950943E-5
70-71	2.422897936151309E-6
72-73	0.0
74-75	0.0
76-77	2.311187356326001E-5
78-79	0.0
80-81	8.008009076811355E-6
82-83	2.7308007977761448E-6
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.06798551591766E-6
92-93	1.0543122569390919E-4
94-95	8.386529650005294E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	6723.0
20-21	19441.0
22-23	31386.0
24-25	54789.0
26-27	60232.0
28-29	70440.0
30-31	93098.0
32-33	108640.0
34-35	148555.0
36-37	157014.0
38-39	173622.0
40-41	201553.0
42-43	229772.0
44-45	260822.0
46-47	268223.0
48-49	244894.0
50-51	223777.0
52-53	210840.0
54-55	223781.0
56-57	263251.0
58-59	286862.0
60-61	297159.0
62-63	321073.0
64-65	336583.0
66-67	356632.0
68-69	374759.0
70-71	390174.0
72-73	389043.0
74-75	404422.0
76-77	332261.0
78-79	383591.0
80-81	418163.0
82-83	431008.0
84-85	455996.0
86-87	505077.0
88-89	572880.0
90-91	628782.0
92-93	711453.0
94-95	862479.0
96-97	1367511.0
98-99	1675083.0
100-101	1.1205738E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.44344518210697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.17696185134452	82.40891517734661
2	4.407499566320235	7.79628892568008
3	1.2076889994212272	3.204365274520348
4	0.5094878439678516	1.8024344079568222
5	0.24872795822075505	1.0999178769077371
6	0.15311383279065063	0.8125148926225323
7	0.09060570976222705	0.5609436787178996
8	0.05359068880991644	0.3791796118424958
9	0.031854152369373986	0.25355618810128905
>10	0.11874511403933283	1.5490240445194878
>50	0.0014695846799692865	0.08502469441724103
>100	2.2662755975758723E-4	0.03074802167866578
>500	2.8070714169672216E-5	0.017087205688759724
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.552940800110818E-5	0.0	0.0	0.0
3	0.0	1.552940800110818E-5	0.0	0.0	0.0
4	0.0	6.211763200443272E-5	0.0	0.0	0.0
5	3.882352000277045E-6	6.211763200443272E-5	0.0	0.0	0.0
6	3.882352000277045E-6	6.211763200443272E-5	0.0	0.0	3.882352000277045E-6
7	3.882352000277045E-6	6.599998400470976E-5	0.0	0.0	3.882352000277045E-6
8	3.882352000277045E-6	8.152939200581794E-5	0.0	3.882352000277045E-6	3.882352000277045E-6
9	3.882352000277045E-6	1.902352480135752E-4	0.0	3.882352000277045E-6	3.882352000277045E-6
10-11	3.882352000277045E-6	1.902352480135752E-4	0.0	7.76470400055409E-6	3.882352000277045E-6
12-13	3.882352000277045E-6	1.9605877601399076E-4	0.0	7.76470400055409E-6	5.823528000415567E-6
14-15	3.882352000277045E-6	2.5429405601814646E-4	0.0	7.76470400055409E-6	1.1647056000831134E-5
16-17	3.882352000277045E-6	2.7176464001939314E-4	0.0	1.9411760001385224E-5	2.329411200166227E-5
18-19	3.882352000277045E-6	3.2029404002285617E-4	0.0	2.7176464001939314E-5	2.329411200166227E-5
20-21	3.882352000277045E-6	3.785293200270119E-4	0.0	4.658822400332454E-5	2.329411200166227E-5
22-23	3.882352000277045E-6	4.1347048802950525E-4	0.0	1.4170584801011214E-4	3.299999200235488E-5
24-25	3.882352000277045E-6	4.484116560319987E-4	0.0	4.0958813602922826E-4	5.2411752003740105E-5
26-27	7.76470400055409E-6	4.658822400332454E-4	0.0	0.0010928820880779881	5.435292800387863E-5
28-29	7.76470400055409E-6	4.658822400332454E-4	0.0	0.005827410352415845	5.629410400401715E-5
30-31	7.76470400055409E-6	4.891763520349076E-4	0.0	0.014780114065054709	5.823528000415567E-5
32-33	7.76470400055409E-6	4.949998800353231E-4	0.0	0.03277093323433854	6.211763200443272E-5
34-35	7.76470400055409E-6	5.668233920404485E-4	0.0	0.05501875137192613	6.599998400470976E-5
36-37	7.76470400055409E-6	6.250586720446042E-4	0.0	0.09072086036647384	6.98823360049868E-5
38-39	7.76470400055409E-6	6.54176312046682E-4	0.0	0.16669654783589546	7.76470400055409E-5
40-41	7.76470400055409E-6	7.43470408053054E-4	0.0	0.24868211620174596	8.152939200581794E-5
42-43	7.76470400055409E-6	7.687056960548549E-4	0.0	0.31662909973459463	8.152939200581794E-5
44-45	7.76470400055409E-6	8.638233200616424E-4	0.0	0.4056553134529476	8.735292000623351E-5
46-47	7.76470400055409E-6	9.356468320667677E-4	0.0	0.49352652745121806	9.705880000692612E-5
48-49	7.76470400055409E-6	9.395291840670447E-4	0.0	0.5728449199928782	1.0094115200720317E-4
50-51	7.76470400055409E-6	9.783527040698153E-4	0.0	0.6556865469747898	1.0482350400748021E-4
52-53	7.76470400055409E-6	9.861174080703693E-4	0.0	0.8069856867775864	1.222940880087269E-4
54-55	7.76470400055409E-6	9.899997600706463E-4	0.0	0.9761844104776605	1.4558820001038918E-4
56-57	7.76470400055409E-6	0.0010094115200720317	0.0	1.1500943683300706	1.5141172801080475E-4
58-59	7.76470400055409E-6	0.0010094115200720317	0.0	1.256488283721663	1.5141172801080475E-4
60-61	7.76470400055409E-6	0.0010307644560735552	0.0	1.3372101465114232	1.5141172801080475E-4
62-63	7.76470400055409E-6	0.0010559997440753562	0.0	1.3991530726758437	1.552940800110818E-4
64-65	7.76470400055409E-6	0.0011569408960825592	0.0	1.412642304700806	1.5917643201135884E-4
66-67	7.76470400055409E-6	0.0011647056000831134	0.0	1.4218784201094654	1.6694113601191293E-4
68-69	7.76470400055409E-6	0.0011821761840843602	0.0	1.4307709473660999	1.727646640123285E-4
70-71	7.76470400055409E-6	0.0012015879440857454	0.0	1.438706474854666	1.8635289601329815E-4
72-73	7.76470400055409E-6	0.0012074114720861608	0.0	1.445446237927147	1.902352480135752E-4
74-75	7.76470400055409E-6	0.0012307055840878232	0.0	1.4506699425435197	1.902352480135752E-4
76-77	7.76470400055409E-6	0.0012695291040905935	0.0	1.454946353271825	1.9799995201412929E-4
78-79	7.76470400055409E-6	0.0013005879200928099	0.0	1.4584967641760782	2.0576465601468338E-4
80-81	7.76470400055409E-6	0.0013025290960929485	0.0	1.4598206462081729	2.077058320148219E-4
82-83	7.76470400055409E-6	0.001304470272093087	0.0	1.4612338223362737	2.0964700801496042E-4
84-85	7.76470400055409E-6	0.001304470272093087	0.0	1.4620122339123292	2.0964700801496042E-4
86-87	7.76470400055409E-6	0.001308352624093364	0.0	1.4624179396963581	2.0964700801496042E-4
88	7.76470400055409E-6	0.0013238820320944722	0.0	1.4624470573363602	2.0964700801496042E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	29360	0.0	48.35741	1
GTATCAA	48730	0.0	30.666288	1
ATCAACG	66600	0.0	26.218876	2
TCAACGC	67450	0.0	25.85877	3
TATCAAC	69845	0.0	25.278074	1
CAACGCA	68955	0.0	25.181173	4
AACGCAG	71395	0.0	24.411804	5
ACGCAGA	82470	0.0	20.948965	6
CGCAGAG	84230	0.0	20.698092	7
CAGAGTA	90320	0.0	18.453566	9
GCAGAGT	91160	0.0	18.048859	8
AGAGTAC	83665	0.0	17.676966	10-11
TATACCG	2305	0.0	15.931437	5
GAGTACT	62135	0.0	15.623951	12-13
AGTACTT	68660	0.0	15.611614	12-13
GTACATG	54360	0.0	14.616973	1
TACATGG	54555	0.0	14.247444	2
GTACTTT	70265	0.0	13.873505	14-15
GTGGTAT	13605	0.0	13.84355	1
TATACTG	16170	0.0	13.476223	5
>>END_MODULE