FastQCFastQC Report
Fri 27 May 2016
SRR2139187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31839222
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA361450.052.339481
GTATCAA650450.035.0293771
TCAACGC850700.026.1018124
ATCAACG855900.025.9481353
CAACGCA869200.025.546265
TATCAAC888250.025.2023722
AACGCAG893350.024.935566
ACGCAGA1037050.021.4519987
GTACATG966300.019.595261
ATACAAC317050.019.4918736
CGCAGAG1149300.019.3970038
TATATAC430550.019.3167673
TACATGG966200.019.1236522
ACATGGG974450.018.5648063
AGAGTAC1112450.018.56015410-11
TATACAA380000.018.4504265
GCAGAGT1230350.017.9213039
CATATAT417700.017.4590991
GAGTACT685200.017.41635912-13
CAGAGTA1190350.017.02104210-11
Produced by FastQC (version 0.11.5)