##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31839222
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.749081306069602	31.0	31.0	34.0	30.0	34.0
2	31.910664745514197	31.0	31.0	34.0	30.0	34.0
3	31.96530917118515	31.0	31.0	34.0	30.0	34.0
4	35.69082130838498	37.0	35.0	37.0	33.0	37.0
5	35.54304065595573	37.0	35.0	37.0	33.0	37.0
6	35.54282940707534	37.0	35.0	37.0	33.0	37.0
7	35.42110557852199	37.0	35.0	37.0	33.0	37.0
8	35.414806178367044	37.0	35.0	37.0	33.0	37.0
9	37.004623071505954	39.0	37.0	39.0	33.0	39.0
10-11	36.8658410371962	39.0	37.0	39.0	33.0	39.0
12-13	36.818796985680116	39.0	37.0	39.0	32.0	39.0
14-15	37.94064363444559	40.0	37.0	41.0	33.0	41.0
16-17	37.9353956261871	40.0	37.0	41.0	33.0	41.0
18-19	37.95843898132938	40.0	37.0	41.0	33.0	41.0
20-21	37.93882989058162	40.0	37.0	41.0	33.0	41.0
22-23	37.83569077597928	40.0	37.0	41.0	32.0	41.0
24-25	37.73230835100379	39.5	37.0	41.0	32.0	41.0
26-27	37.5510697598818	39.0	36.5	41.0	32.0	41.0
28-29	37.40498743355312	39.0	36.0	41.0	32.0	41.0
30-31	37.25690912630221	39.0	36.0	40.0	31.0	41.0
32-33	37.417806622213334	39.0	36.0	40.5	32.0	41.0
34-35	37.37137120059862	39.0	36.0	41.0	31.0	41.0
36-37	37.224508464410974	39.0	36.0	40.5	31.0	41.0
38-39	37.04339049140399	39.0	36.0	40.0	31.0	41.0
40-41	36.962050226097446	39.0	35.0	40.0	31.0	41.0
42-43	36.83462108365997	39.0	35.0	40.0	30.0	41.0
44-45	36.8994708576788	39.0	35.0	40.0	30.5	41.0
46-47	37.066855426359666	39.0	36.0	40.0	31.0	41.0
48-49	36.98085179713914	39.0	35.0	40.0	31.0	41.0
50-51	36.82531791623305	39.0	35.0	40.0	30.0	41.0
52-53	36.66543174579836	39.0	35.0	40.0	30.0	41.0
54-55	36.51448988421504	38.0	35.0	40.0	30.0	41.0
56-57	36.342312878335086	38.0	35.0	40.0	30.0	41.0
58-59	36.182599392337394	38.0	34.0	40.0	29.5	41.0
60-61	35.93394266222123	38.0	34.0	40.0	29.0	41.0
62-63	35.651364790288504	37.0	34.0	40.0	28.5	41.0
64-65	35.40733147251076	37.0	34.0	39.5	28.0	41.0
66-67	35.128180803948204	36.0	33.5	39.0	28.0	41.0
68-69	34.8370834266439	36.0	33.0	39.0	28.0	40.0
70-71	34.521607547374316	35.5	33.0	38.5	28.0	40.0
72-73	34.196429993568046	35.0	33.0	38.0	28.0	40.0
74-75	33.8659536618042	35.0	32.0	37.0	27.0	39.5
76-77	32.777546712113484	34.0	31.0	36.0	26.0	39.0
78-79	33.18497115381961	34.5	32.0	36.0	26.0	39.0
80-81	33.08407692034521	34.5	32.0	36.0	26.0	38.0
82-83	32.85824038570077	34.0	32.0	35.5	26.0	37.0
84-85	32.65706049259809	34.0	31.0	35.0	26.0	37.0
86-87	32.45951513133171	34.0	31.0	35.0	26.0	36.0
88-89	32.24312070106396	34.0	31.0	35.0	26.0	36.0
90-91	32.11626588895213	34.0	31.0	35.0	26.0	36.0
92-93	32.04189585144198	34.0	31.0	35.0	26.0	35.0
94-95	32.02092900108812	34.0	31.0	35.0	26.0	35.0
96-97	31.942355422116723	34.0	31.0	35.0	26.0	35.0
98-99	32.135805299125096	34.0	31.0	35.0	26.5	35.0
100-101	31.38819193709703	33.0	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.0
16	0.0
17	2.0
18	7.0
19	61.0
20	314.0
21	2101.0
22	9533.0
23	31729.0
24	80595.0
25	166554.0
26	292170.0
27	448495.0
28	624808.0
29	811213.0
30	1006550.0
31	1227962.0
32	1497223.0
33	1864299.0
34	2410610.0
35	3240897.0
36	4604696.0
37	6183425.0
38	5255461.0
39	2029480.0
40	51033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.50027560346795	22.469006937418257	15.6081106504424	24.422606808671393
2	14.483521696644235	23.213064370677085	39.572894704243346	22.730519228435334
3	17.123750699687324	27.456616245208505	31.519005081217124	23.90062797388705
4	12.500252047615987	19.704850200171347	38.33915288507992	29.455744867132744
5	12.904467326494348	38.189997230459966	35.98444396662707	12.921091476418614
6	29.495953136040825	39.16189283770816	18.460714900634194	12.881439125616826
7	25.71974277512183	32.70349068202734	24.072968868397602	17.503797674453224
8	24.40680868395591	37.11668896934731	21.042307503619277	17.43419484307751
9	25.44693774238579	15.851850902638262	21.21549640880044	37.485714946175506
10-11	24.180250070180733	26.954069417902232	29.12060005737577	19.74508045454126
12-13	24.884023548062824	24.580729390938007	29.070762156185854	21.464484904813315
14-15	22.019545578092327	25.704651325965187	26.586273056546418	25.689530039396065
16-17	21.7371187650251	28.761216275950463	27.179781591396925	22.321883367627514
18-19	21.905422949862093	27.97041639889528	28.67905243893819	21.445108212304437
20-21	22.797916150162905	27.108726040311215	28.51188713285201	21.581470676673874
22-23	22.521143435961257	27.11225923776004	28.351843022023704	22.014754304255
24-25	22.320826157809293	27.2591662746793	28.48025629610696	21.93975127140445
26-27	22.239388323150205	27.268621694905537	28.42133662020397	22.07065336174029
28-29	22.307007281261466	27.324711250301025	28.37950035484379	21.98878111359372
30-31	22.60768222278721	27.014332728943995	28.336059242311624	22.041925805957167
32-33	22.462203606634855	27.1698228505367	28.21065736274483	22.15731618008362
34-35	22.537316683760793	27.24711436334779	28.226408707641465	21.98916024524995
36-37	22.62704111774291	27.12467926991132	28.10045235997253	22.147827252373244
38-39	22.298257977374696	27.385915349432675	28.185545094053822	22.130281579138806
40-41	22.43654939766738	27.16651642423925	28.18288566374063	22.214048514352744
42-43	22.28354324702514	27.39786349363042	27.871031229099746	22.44756203024469
44-45	22.325559767283558	27.36542902161696	27.77994657518446	22.52906463591502
46-47	22.474881824903708	27.318683217173113	27.66578260080686	22.54065235711632
48-49	22.582506724067457	27.57695368025664	27.416876354545238	22.42366324113066
50-51	22.547835079461596	27.532334804589087	27.403878966579136	22.51595114937018
52-53	22.701728442716938	27.434152479163142	27.333308270285663	22.53081080783426
54-55	22.681220714200823	27.361532952601348	27.345429976614106	22.611816356583724
56-57	22.683553315042612	27.565929093305392	27.165935218711745	22.58458237294025
58-59	22.54058186783459	27.514440963255478	27.387579279828326	22.557397889081606
60-61	22.65600947237214	27.512638006035207	27.234555951971164	22.596796569621496
62-63	22.59515588811687	27.533482985925527	27.394391590949578	22.476969535008017
64-65	22.667355011886237	27.45201692009738	27.309266495495084	22.571361572521305
66-67	22.713950341848932	27.608126773513526	27.016934193682363	22.66098869095518
68-69	22.659463026989616	27.51249239024549	27.090384879349788	22.737659703415105
70-71	22.661030981693273	27.372823860834718	27.13526821073448	22.830876946737526
72-73	22.61037812527157	27.434894139729458	27.023223619278276	22.931504115720696
74-75	22.528790248463302	27.419807123127484	27.055843289123672	22.995559339285546
76-77	22.693283433505687	27.486576531477102	26.96488007809495	22.855259956922264
78-79	22.55437588529645	27.502165596718047	26.99723687561256	22.946221642372937
80-81	22.618225904349277	27.40851312708784	27.053800332510843	22.919460636052044
82-83	22.751150923705605	27.271244830268536	27.25412434880081	22.72347989722504
84-85	22.667195180712437	27.34493508463544	27.194889313266195	22.79298042138593
86-87	22.49757571945019	27.540928394412607	27.1381647913019	22.8233310948353
88-89	22.707113776449123	27.35198507581755	27.067145771533426	22.87375537619991
90-91	22.680139665445925	27.386055982092376	27.148589731112306	22.785214621349393
92-93	22.7088469634953	27.324815037126115	27.273278466001333	22.69305953337725
94-95	22.34953140513653	27.447883804311168	27.406822496369887	22.795762294182413
96-97	22.228637262647663	27.48947317097311	27.51396572771506	22.767923838664164
98-99	22.44645040238982	28.11307689810057	27.59907272269613	21.841399976813484
100-101	22.826047446150625	30.929192504936303	24.853872490749396	21.390887558163676
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8754.0
1	7619.0
2	9548.0
3	13196.5
4	16791.0
5	24609.0
6	31306.5
7	38000.0
8	43769.5
9	44662.5
10	41516.5
11	37694.0
12	41128.0
13	54925.0
14	88104.0
15	134318.0
16	168861.0
17	182357.5
18	181898.5
19	173836.0
20	161495.5
21	150761.5
22	148515.5
23	162354.0
24	193020.5
25	239539.5
26	298185.5
27	359779.5
28	423181.5
29	486624.5
30	545010.5
31	604184.0
32	667750.0
33	731249.5
34	792294.0
35	854477.0
36	923552.5
37	979468.0
38	1021662.5
39	1065117.5
40	1107955.0
41	1147476.5
42	1172514.0
43	1197111.5
44	1227121.5
45	1265714.5
46	1312010.0
47	1355211.0
48	1377567.0
49	1371699.0
50	1344533.0
51	1321792.5
52	1263984.0
53	1170445.5
54	1103038.5
55	1040319.5
56	968589.0
57	882752.0
58	788336.5
59	683476.0
60	579404.0
61	491050.0
62	404234.5
63	327111.0
64	265597.0
65	211978.5
66	168696.5
67	121651.5
68	80752.5
69	60565.5
70	43868.0
71	29048.5
72	19980.5
73	14372.5
74	11155.5
75	8359.0
76	5428.5
77	3067.5
78	2288.5
79	1894.5
80	1324.0
81	865.5
82	659.5
83	423.0
84	261.5
85	179.5
86	126.5
87	96.5
88	65.0
89	43.0
90	28.0
91	19.0
92	14.0
93	8.0
94	4.5
95	3.0
96	3.0
97	3.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0023555851961458104
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	8.951223745354079E-5
20-21	0.0019411212689128582
22-23	3.518422238941709E-4
24-25	3.9279900131011036E-5
26-27	2.687981595720117E-4
28-29	0.003518351213488298
30-31	3.369779652313064E-4
32-33	4.7345931485986626E-5
34-35	0.0019089841924711944
36-37	0.05314667807508204
38-39	0.028552793472020006
40-41	0.04125211846464436
42-43	8.177103338370581E-4
44-45	0.002293047623636676
46-47	0.0014167998271140505
48-49	4.808457515783553E-4
50-51	1.011118188189452E-5
52-53	8.735732797795523E-4
54-55	0.002096992157711902
56-57	9.78257923820048E-4
58-59	0.003599315540338827
60-61	0.001304464913692635
62-63	8.729726923986829E-5
64-65	6.338180955390388E-4
66-67	3.151384993629284E-4
68-69	8.483194340189777E-5
70-71	8.146471159829167E-4
72-73	0.0015240723440223185
74-75	3.4529187810199196E-4
76-77	5.832473966898261E-4
78-79	8.476681074198933E-5
80-81	0.0020077641822316434
82-83	0.002420444317505587
84-85	0.003571062268889705
86-87	0.0036759310919079035
88-89	0.06816665425774032
90-91	5.795666875128421E-4
92-93	5.37749246495673E-5
94-95	4.609225909546615E-5
96-97	0.007578344912943762
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1230.0
20-21	3873.0
22-23	7551.0
24-25	14213.0
26-27	16902.0
28-29	27237.0
30-31	66724.0
32-33	86491.0
34-35	121567.0
36-37	186841.0
38-39	204666.0
40-41	223711.0
42-43	256500.0
44-45	300174.0
46-47	303237.0
48-49	282202.0
50-51	258549.0
52-53	230802.0
54-55	251309.0
56-57	265620.0
58-59	290635.0
60-61	302767.0
62-63	291430.0
64-65	313635.0
66-67	332940.0
68-69	349062.0
70-71	354532.0
72-73	343640.0
74-75	348917.0
76-77	344760.0
78-79	380749.0
80-81	397003.0
82-83	387801.0
84-85	397192.0
86-87	429843.0
88-89	473608.0
90-91	535169.0
92-93	587120.0
94-95	779084.0
96-97	968039.0
98-99	2878085.0
100-101	1.7243812E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.36676648263273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.50906215700547	72.0169619212589
2	7.580640802544021	12.336244599386339
3	1.9750585780410663	4.821123903269642
4	0.8094590625246587	2.6345226607077885
5	0.40204470264948255	1.6356538718029976
6	0.20928044784288224	1.0217083997407557
7	0.12853647533219512	0.732101816099878
8	0.0886225334884131	0.5768743189960805
9	0.0585350119397009	0.42865241828001854
>10	0.22917780539552113	2.9598850228161226
>50	0.0068824244766767	0.3799515580622871
>100	0.0025978115804558186	0.37338435429691386
>500	5.6169990618219626E-5	0.024780541369158204
>1k	4.601718885935234E-5	0.05815461391314698
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.140780261527747E-6	6.281560523055494E-6
2	0.0	4.397092366138846E-5	0.0	9.422340784583242E-6	6.281560523055494E-6
3	0.0	4.711170392291621E-5	0.0	9.422340784583242E-6	6.281560523055494E-6
4	0.0	1.193496499380544E-4	0.0	1.5703901307638734E-5	6.281560523055494E-6
5	3.140780261527747E-6	1.2563121046110987E-4	0.0	1.5703901307638734E-5	9.422340784583242E-6
6	2.198546183069423E-5	1.2563121046110987E-4	0.0	1.5703901307638734E-5	2.5126242092221978E-5
7	2.198546183069423E-5	1.2877199072263763E-4	0.0	1.8844681569166483E-5	2.8267022353749723E-5
8	3.454858287680522E-5	1.4447589203027636E-4	0.0	3.140780261527747E-5	3.7689363138332966E-5
9	3.454858287680522E-5	2.7638866301444177E-4	0.0	3.7689363138332966E-5	3.7689363138332966E-5
10-11	5.0252484184443955E-5	2.7638866301444177E-4	0.0	4.71117039229162E-5	4.083014339986071E-5
12-13	5.0252484184443955E-5	2.8109983340673337E-4	0.0	6.281560523055494E-5	4.240053353062459E-5
14-15	5.0252484184443955E-5	3.5961933994492707E-4	0.0	7.223794601513818E-5	5.6534044707499446E-5
16-17	5.0252484184443955E-5	3.721824609910381E-4	0.0	7.69491164074298E-5	6.281560523055494E-5
18-19	5.810443483826332E-5	4.1772377478319035E-4	0.0	8.32306769304853E-5	6.281560523055494E-5
20-21	7.22379460151382E-5	4.7582820962145374E-4	0.0	1.1777925980729052E-4	6.281560523055494E-5
22-23	8.480106706124918E-5	5.559181062904112E-4	0.0	2.60684761706803E-4	6.595638549208269E-5
24-25	1.0364574863041566E-4	6.140225411286745E-4	0.0	8.32306769304853E-4	7.223794601513818E-5
26-27	1.2563121046110987E-4	6.344376128286049E-4	0.0	0.0024906387473915036	7.223794601513818E-5
28-29	1.2877199072263763E-4	6.360080029593688E-4	0.0	0.013968620213144656	7.223794601513818E-5
30-31	1.3348316111492926E-4	6.972532180591598E-4	0.0	0.02135259460799639	7.223794601513818E-5
32-33	1.49187062422568E-4	7.098163391052708E-4	0.0	0.027488108848890844	8.166028679972142E-5
34-35	1.6960213412249835E-4	8.118916976049226E-4	0.0	0.035731086645270416	8.794184732277691E-5
36-37	1.6960213412249835E-4	9.123966659738106E-4	0.0	0.048181139601966405	8.794184732277691E-5
38-39	1.7588369464555386E-4	9.485156389813797E-4	0.0	0.07293205845293582	9.736418810736016E-5
40-41	1.9158759595319256E-4	0.0010113312442119346	0.0	0.1205572799486118	1.0050496836888791E-4
42-43	1.9158759595319256E-4	0.0010537317777425592	0.0	0.14288351643768182	1.0207535849965179E-4
44-45	1.962987663454842E-4	0.001202918840165127	0.0	0.1767426980470817	1.0521613876117953E-4
46-47	2.072914972608313E-4	0.0013097053690570705	0.0	0.21584070113270984	1.1463847954576277E-4
48-49	2.072914972608313E-4	0.001314416539449362	0.0	0.24897593289182757	1.3191277098416538E-4
50-51	2.1043227752235905E-4	0.001451040480825819	0.0	0.2838370234046548	1.3191277098416538E-4
52-53	2.1043227752235905E-4	0.0014604628216104024	0.0	0.32644955960293254	1.4290550189951248E-4
54-55	2.1043227752235905E-4	0.0014730259426565134	0.0	0.4130832719467831	1.554686229456235E-4
56-57	2.2142500843770618E-4	0.0014918706242256798	0.0	0.5026598953956852	1.5860940320715122E-4
58-59	2.386992998761088E-4	0.0014934410143564439	0.0	0.5782945952636657	1.6175018346867897E-4
60-61	2.4498086039916424E-4	0.0015358415478870682	0.0	0.6207343885475594	1.6332057359944285E-4
62-63	2.528328110529836E-4	0.001565678960371582	0.0	0.6439431842901187	1.7588369464555383E-4
64-65	2.544032011837475E-4	0.0017604073365863023	0.0	0.6606222978689618	1.7902447490708158E-4
66-67	2.559735913145114E-4	0.0017886743589400518	0.0	0.669791805842492	1.8687642556090096E-4
68-69	2.5754398144527525E-4	0.0018295045023399127	0.0	0.6787681558299383	1.9158759595319256E-4
70-71	2.60684761706803E-4	0.0018577715246936625	0.0	0.6872985150202477	1.9472837621472032E-4
72-73	2.73247882752914E-4	0.0018703346457397733	0.0	0.6948253949169989	2.0886188739159517E-4
74-75	2.73247882752914E-4	0.001912735179270398	0.0	0.7016691551068679	2.1043227752235905E-4
76-77	2.7638866301444177E-4	0.0019849731252855364	0.0	0.7078297955898545	2.1043227752235905E-4
78-79	2.795294432759695E-4	0.0020038178068547026	0.0	0.7135444452757043	2.1043227752235905E-4
80-81	2.826702235374972E-4	0.0020069585871162303	0.0	0.7179650934938047	2.1671383804541455E-4
82-83	2.842406136682611E-4	0.0020132401476392857	0.0	0.721493760117631	2.2927695909152554E-4
84-85	2.889517840605527E-4	0.002024232878554633	0.0	0.7246188364778512	2.2927695909152554E-4
86-87	2.920925643220805E-4	0.002041507169993036	0.0	0.7251496283420493	2.324177393530533E-4
88-89	2.952333445836082E-4	0.002123167456792757	0.0	0.7252250070683259	2.4184008013763652E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	36145	0.0	52.33948	1
GTATCAA	65045	0.0	35.029377	1
TCAACGC	85070	0.0	26.101812	4
ATCAACG	85590	0.0	25.948135	3
CAACGCA	86920	0.0	25.54626	5
TATCAAC	88825	0.0	25.202372	2
AACGCAG	89335	0.0	24.93556	6
ACGCAGA	103705	0.0	21.451998	7
GTACATG	96630	0.0	19.59526	1
ATACAAC	31705	0.0	19.491873	6
CGCAGAG	114930	0.0	19.397003	8
TATATAC	43055	0.0	19.316767	3
TACATGG	96620	0.0	19.123652	2
ACATGGG	97445	0.0	18.564806	3
AGAGTAC	111245	0.0	18.560154	10-11
TATACAA	38000	0.0	18.450426	5
GCAGAGT	123035	0.0	17.921303	9
CATATAT	41770	0.0	17.459099	1
GAGTACT	68520	0.0	17.416359	12-13
CAGAGTA	119035	0.0	17.021042	10-11
>>END_MODULE