FastQCFastQC Report
Fri 27 May 2016
SRR2139186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7235382
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74750.048.5358161
GTATCAA131050.033.369121
TCAACGC169000.025.202934
ATCAACG170300.024.9611153
CAACGCA173050.024.6130925
ACCGAGG60900.024.38070992-93
GACCGAG60100.024.3594690-91
GAGACCG59450.024.17998988-89
CGAGACC61850.023.78567588-89
TATCAAC180750.023.704772
AACGCAG179500.023.681776
AGACCGA62100.023.4728890-91
ACGAGAC65700.022.61548286-87
CACGAGA68150.021.58399286-87
GCCCACG70950.020.76119882-83
ACGCAGA209100.020.3696587
GTACATG201100.020.3223271
TACATGG198400.020.238252
CCCACGA72100.020.20556384-85
CCACGAG74200.020.02559784-85
Produced by FastQC (version 0.11.5)