##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7235382
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.805531345822516	31.0	31.0	34.0	30.0	34.0
2	31.958743159656255	31.0	31.0	34.0	30.0	34.0
3	32.01900922439202	33.0	31.0	34.0	30.0	34.0
4	35.729498456335826	37.0	35.0	37.0	33.0	37.0
5	35.58292374887739	37.0	35.0	37.0	33.0	37.0
6	35.58258610257206	37.0	35.0	37.0	33.0	37.0
7	35.46299836000366	37.0	35.0	37.0	33.0	37.0
8	35.45466666445531	37.0	35.0	37.0	33.0	37.0
9	37.05311661499006	39.0	37.0	39.0	33.0	39.0
10-11	36.92157615174983	39.0	37.0	39.0	33.0	39.0
12-13	36.878284380838494	39.0	37.0	39.0	33.0	39.0
14-15	38.01847967391355	40.0	37.0	41.0	33.0	41.0
16-17	38.01165646817265	40.0	37.0	41.0	33.0	41.0
18-19	38.034597551311045	40.0	37.0	41.0	33.0	41.0
20-21	38.01819469859279	40.0	37.0	41.0	33.0	41.0
22-23	37.9212765219455	40.0	37.0	41.0	33.0	41.0
24-25	37.816922353986044	40.0	37.0	41.0	32.0	41.0
26-27	37.643840126787154	39.5	37.0	41.0	32.0	41.0
28-29	37.503381992906874	39.0	36.5	41.0	32.0	41.0
30-31	37.36276825939396	39.0	36.0	40.5	31.5	41.0
32-33	37.52188205010237	39.0	36.0	41.0	32.0	41.0
34-35	37.48309223141716	39.0	36.0	41.0	32.0	41.0
36-37	37.33961916843933	39.0	36.0	41.0	31.0	41.0
38-39	37.17089175458224	39.0	36.0	40.0	31.0	41.0
40-41	37.09638167992906	39.0	36.0	40.0	31.0	41.0
42-43	36.97504251668167	39.0	35.0	40.0	31.0	41.0
44-45	37.040947485476266	39.0	35.5	40.0	31.0	41.0
46-47	37.19928656658071	39.0	36.0	41.0	31.0	41.0
48-49	37.119539062150395	39.0	36.0	41.0	31.0	41.0
50-51	36.97023884577534	39.0	35.0	40.5	31.0	41.0
52-53	36.81620627899916	39.0	35.0	40.0	30.0	41.0
54-55	36.67189091394593	39.0	35.0	40.0	30.0	41.0
56-57	36.5018266349868	38.0	35.0	40.0	30.0	41.0
58-59	36.34639759324702	38.0	35.0	40.0	30.0	41.0
60-61	36.10918800539869	38.0	34.0	40.0	29.0	41.0
62-63	35.82547353291816	37.5	34.0	40.0	29.0	41.0
64-65	35.589000920882846	37.0	34.0	40.0	29.0	41.0
66-67	35.318171670291036	36.5	34.0	39.0	28.5	41.0
68-69	35.02530829406387	36.0	33.5	39.0	28.0	40.5
70-71	34.713454334035774	36.0	33.0	39.0	28.0	40.0
72-73	34.38397266824539	35.0	33.0	38.0	28.0	40.0
74-75	34.05160300450442	35.0	32.5	37.5	28.0	39.5
76-77	32.954905087430745	34.0	31.0	36.0	26.0	39.0
78-79	33.35635003233649	35.0	32.0	36.0	27.0	39.0
80-81	33.241279651392276	35.0	32.0	36.0	27.0	38.5
82-83	33.00966311611154	34.5	32.0	35.5	27.0	37.0
84-85	32.81376719267877	34.0	32.0	35.0	27.0	37.0
86-87	32.61877120079386	34.0	31.5	35.0	26.0	36.5
88-89	32.39985899338652	34.0	31.0	35.0	26.0	36.0
90-91	32.2679717675457	34.0	31.0	35.0	26.0	36.0
92-93	32.20227123417243	34.0	31.0	35.0	26.0	35.0
94-95	32.167591723139225	34.0	31.0	35.0	26.0	35.0
96-97	32.08589863996525	34.0	31.0	35.0	26.0	35.0
98-99	32.26473358728017	34.0	31.0	35.0	27.0	35.0
100-101	31.520466577124015	33.0	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	12.0
20	61.0
21	406.0
22	1967.0
23	6749.0
24	17029.0
25	35746.0
26	62265.0
27	97332.0
28	135512.0
29	176766.0
30	220445.0
31	267860.0
32	327523.0
33	407249.0
34	527759.0
35	708272.0
36	1014895.0
37	1418815.0
38	1285371.0
39	508072.0
40	15275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.65017520844097	22.34568955723416	15.451209072306066	24.552926162018814
2	14.325294733754848	22.944658967427735	39.84712738076514	22.88291891805228
3	17.016544530751798	27.327928228253878	31.431415784266814	24.22411145672751
4	12.361005956561796	19.491673003581568	38.61613664627521	29.531184393581434
5	12.8599568067035	38.1954815930935	36.04641745245794	12.898144147745066
6	29.453938990367064	39.08352040016685	18.631207032330842	12.83133357713525
7	25.64833480803087	32.69505328122275	24.193567112282395	17.463044798463994
8	24.269084894204617	37.05098086044386	21.26360432662712	17.4163299187244
9	25.360402532996872	16.042594572062676	21.344761064446907	37.25224183049354
10-11	24.05473546524565	26.90676870965486	29.31505896993414	19.723436855165353
12-13	24.760835018800666	24.554674514766464	29.212638669250634	21.471851797182236
14-15	21.92798528121943	25.734729693608436	26.66982890467981	25.667456120492325
16-17	21.717153289211268	28.720992201932116	27.281897486545976	22.27995702231064
18-19	21.846563967983133	28.056208846716757	28.699057484731092	21.398169700569017
20-21	22.670359719499192	27.21568829631538	28.516379513563905	21.59757247062152
22-23	22.385042318825434	27.185058022543824	28.366873420772354	22.063026237858388
24-25	22.203201800665457	27.331573744609543	28.489103902617163	21.976120552107837
26-27	22.163199607789622	27.338558885542618	28.399561834383107	22.098679672284653
28-29	22.22226451618038	27.390124045487845	28.37734577190953	22.010265666422246
30-31	22.47590566599237	27.178810635978166	28.282810785642106	22.062472912387356
32-33	22.28921071855139	27.340420042779705	28.20110447221594	22.16926476645297
34-35	22.389677111981854	27.388083794234593	28.22631868374889	21.995920410034664
36-37	22.483648081604006	27.244150060687605	28.1339573007307	22.13824455697769
38-39	22.206939857055357	27.514376035793127	28.165496795902012	22.11318731124951
40-41	22.364454052165804	27.281988197209195	28.15645120085018	22.197106549774823
42-43	22.213338853712337	27.53116277741689	27.875812887705496	22.379685481165275
44-45	22.229239893760884	27.50714976794398	27.8260845770958	22.437525761199336
46-47	22.388440470106787	27.456705178506663	27.657095516048933	22.49775883533761
48-49	22.41201413531361	27.688492879467958	27.467657979460906	22.431835005757524
50-51	22.38859580366193	27.680925618119524	27.423312042189448	22.5071665360291
52-53	22.557706083115182	27.594177801852872	27.337013572739277	22.511102542292676
54-55	22.56262446663616	27.4934175501047	27.350734997531017	22.593222985728126
56-57	22.549963329492023	27.623785301304586	27.27193852585699	22.554312843346402
58-59	22.422618465016573	27.628143345980604	27.407478203127848	22.54175998587497
60-61	22.442846766339656	27.491501508568177	27.429194946686753	22.63645677840542
62-63	22.65010915815913	27.529249709722293	27.47286115243001	22.347779979688564
64-65	22.443810108767455	27.468895966599323	27.437759581658288	22.649534342974935
66-67	22.47808759216142	27.588282199725583	27.218032149638937	22.71559805847406
68-69	22.44070051308885	27.712849618637513	27.24706849657744	22.599381371696197
70-71	22.43632232958786	27.548903458835284	27.293886652248784	22.720887559328077
72-73	22.423746355249932	27.612029416740473	26.98001565708334	22.984208570926256
74-75	22.337630808080384	27.571936568634285	27.035700094674937	23.054732528610394
76-77	22.488694989396617	27.359415277944123	27.207393604193925	22.944496128465335
78-79	22.351745854869783	27.40907052632996	27.164743359385923	23.074440259414335
80-81	22.694522664994636	27.352713415344727	26.9747371523732	22.97802676728743
82-83	22.70660253568429	27.48081602079543	27.151406157617426	22.661175285902857
84-85	22.474167511846453	27.314468986775957	27.09208100816607	23.119282493211514
86-87	22.3410056101692	27.640866088883175	27.080731458791956	22.937396842155668
88-89	22.721278147578328	27.22535894003912	27.037390001037664	23.015972911344893
90-91	22.71876985593719	27.483229898160864	27.095939111850708	22.702061134051238
92-93	22.461869476065345	27.40163930258852	27.19982588569701	22.93666533564912
94-95	22.426269766380383	27.24422108412568	27.36872620264712	22.960782946846813
96-97	22.32433956840888	27.54610725280065	27.459731521599508	22.669821657190965
98-99	22.594679417353344	27.99796335613136	27.59524016975575	21.812117056759547
100-101	22.775108561014303	30.917872356378247	24.895136694307357	21.41188238830009
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1874.0
1	1631.5
2	2057.5
3	2814.0
4	3579.0
5	5403.5
6	6951.0
7	8317.0
8	9443.5
9	9599.0
10	8988.0
11	8276.5
12	8987.0
13	11794.0
14	18481.5
15	27915.0
16	35293.5
17	38378.0
18	38528.5
19	37351.5
20	35381.0
21	33708.0
22	34041.0
23	37717.0
24	45204.0
25	56317.0
26	69887.5
27	84141.0
28	98559.0
29	113359.0
30	126695.0
31	140140.0
32	154924.0
33	169127.0
34	183307.0
35	197614.5
36	212246.0
37	224964.0
38	235591.0
39	245719.5
40	254346.5
41	262917.0
42	269344.0
43	275341.5
44	282106.5
45	289842.0
46	298267.5
47	305063.0
48	308702.5
49	307316.0
50	302036.0
51	295544.0
52	282154.5
53	263505.5
54	248570.5
55	233270.5
56	217205.0
57	199389.0
58	178165.5
59	153759.0
60	129578.5
61	108972.5
62	89127.0
63	71355.0
64	56662.0
65	45055.5
66	36109.5
67	26145.0
68	17794.5
69	13469.0
70	9751.5
71	6406.5
72	4434.0
73	3197.5
74	2416.5
75	1743.5
76	1133.5
77	689.0
78	511.5
79	414.0
80	286.0
81	182.0
82	132.5
83	84.5
84	50.5
85	32.5
86	22.5
87	19.0
88	20.0
89	19.5
90	16.0
91	11.0
92	7.5
93	4.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002874761830128665
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.1747824786583486E-4
20-21	0.0021216312538536635
22-23	3.870619832074541E-4
24-25	2.7654886376442462E-5
26-27	2.9050486910369355E-4
28-29	0.003667905103682335
30-31	3.810872627471957E-4
32-33	7.638979071280703E-5
34-35	0.0018597067848372187
36-37	0.055288849965339545
38-39	0.02821989414548063
40-41	0.040976535388938744
42-43	0.0010707517033875015
44-45	0.0025418510738816737
46-47	0.0014762706581534575
48-49	5.874991921886107E-4
50-51	0.0
52-53	0.001083965378594345
54-55	0.0024568650612837107
56-57	0.0010949705190593686
58-59	0.003922611406002102
60-61	0.0015373608427703984
62-63	1.7177321095272839E-4
64-65	8.28508193669866E-4
66-67	4.0707704236339035E-4
68-69	9.695852421369464E-5
70-71	9.903944020616573E-4
72-73	0.00152587890625
74-75	4.36830085361639E-4
76-77	7.405670905264104E-4
78-79	5.178065919885999E-5
80-81	0.002137888554673433
82-83	0.002527627907539727
84-85	0.00371536842714262
86-87	0.0038782490789847704
88-89	0.06896456845858809
90-91	6.878328764817424E-4
92-93	4.88865166978253E-5
94-95	6.02686777654785E-5
96-97	0.007719150430928605
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	220.0
20-21	840.0
22-23	1538.0
24-25	3076.0
26-27	3755.0
28-29	6229.0
30-31	15286.0
32-33	19757.0
34-35	27350.0
36-37	42271.0
38-39	46755.0
40-41	50292.0
42-43	57915.0
44-45	67215.0
46-47	68193.0
48-49	63980.0
50-51	58915.0
52-53	53238.0
54-55	57698.0
56-57	61197.0
58-59	40100.0
60-61	69181.0
62-63	66404.0
64-65	70865.0
66-67	75464.0
68-69	78543.0
70-71	80678.0
72-73	77057.0
74-75	78511.0
76-77	77955.0
78-79	86242.0
80-81	88925.0
82-83	86950.0
84-85	88846.0
86-87	95761.0
88-89	105517.0
90-91	117863.0
92-93	129530.0
94-95	168632.0
96-97	213089.0
98-99	673631.0
100-101	3959918.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.15928479397988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.48891585372719	79.7410847238747
2	6.0623348299122135	10.567775359135645
3	1.3505685714610538	3.531437722613177
4	0.4826747326206442	1.682783379333633
5	0.22506389555029968	0.9808204084555556
6	0.1224715642875768	0.6404720370543073
7	0.07069426210492459	0.4313162926872367
8	0.045172021184871035	0.3149728847337494
9	0.031340203290993046	0.24584307337267997
>10	0.11749787737150043	1.6331329993147081
>50	0.00283789804835164	0.1687602189864009
>100	4.282904403331439E-4	0.0616009004382762
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3820970337157043E-5	0.0	0.0	0.0
3	0.0	1.3820970337157043E-5	0.0	0.0	0.0
4	0.0	9.67467923600993E-5	0.0	1.3820970337157043E-5	1.3820970337157043E-5
5	0.0	1.1056776269725634E-4	0.0	1.3820970337157043E-5	1.3820970337157043E-5
6	0.0	1.1056776269725634E-4	0.0	1.3820970337157043E-5	2.7641940674314086E-5
7	0.0	1.1056776269725634E-4	0.0	1.3820970337157043E-5	2.7641940674314086E-5
8	0.0	1.3820970337157044E-4	0.0	1.3820970337157043E-5	4.1462911011471126E-5
9	0.0	2.3495649573166972E-4	0.0	1.3820970337157043E-5	4.1462911011471126E-5
10-11	0.0	2.3495649573166972E-4	0.0	4.837339618004965E-5	4.1462911011471126E-5
12-13	0.0	2.556879512374053E-4	0.0	6.910485168578522E-5	4.1462911011471126E-5
14-15	0.0	3.17882317754612E-4	0.0	8.983630719152077E-5	4.1462911011471126E-5
16-17	0.0	3.17882317754612E-4	0.0	1.1056776269725634E-4	4.1462911011471126E-5
18-19	0.0	3.3861377326034755E-4	0.0	1.4512018854014895E-4	4.1462911011471126E-5
20-21	0.0	3.593452287660831E-4	0.0	1.7967261438304154E-4	4.1462911011471126E-5
22-23	0.0	4.2845008045186834E-4	0.0	2.625984364059838E-4	4.837339618004965E-5
24-25	0.0	5.04465417306232E-4	0.0	6.49585605846381E-4	5.528388134862817E-5
26-27	0.0	5.390178431491247E-4	0.0	0.00214916088742792	5.528388134862817E-5
28-29	1.3820970337157043E-5	5.390178431491247E-4	0.0	0.014235599447271754	5.528388134862817E-5
30-31	1.3820970337157043E-5	6.35764635509224E-4	0.0	0.02158144518147072	5.528388134862817E-5
32-33	2.7641940674314086E-5	6.35764635509224E-4	0.0	0.02630821703677843	5.528388134862817E-5
34-35	5.528388134862817E-5	7.048694871950092E-4	0.0	0.033204881235019794	5.528388134862817E-5
36-37	6.910485168578522E-5	7.739743388807944E-4	0.0	0.04299703871889556	5.528388134862817E-5
38-39	6.910485168578522E-5	7.9470579438653E-4	0.0	0.06331386511451642	5.528388134862817E-5
40-41	6.910485168578522E-5	8.707211312408937E-4	0.0	0.10435523653070425	6.21943665172067E-5
42-43	8.292582202294225E-5	8.983630719152078E-4	0.0	0.1230964723078892	6.910485168578522E-5
44-45	8.292582202294225E-5	0.001008930834612464	0.0	0.15115995257748657	6.910485168578522E-5
46-47	8.292582202294225E-5	0.0010918566566354064	0.0	0.18386036839520015	6.910485168578522E-5
48-49	9.67467923600993E-5	0.0010918566566354064	0.0	0.2111636952962539	1.1056776269725634E-4
50-51	9.67467923600993E-5	0.0011886034489955058	0.0	0.23879872548539938	1.1056776269725634E-4
52-53	9.67467923600993E-5	0.0011886034489955058	0.0	0.2757836421076316	1.3820970337157044E-4
54-55	1.0365727752867783E-4	0.0012162453896698198	0.0	0.35219287661660437	1.5203067370872747E-4
56-57	1.1056776269725634E-4	0.0012162453896698198	0.0	0.43316994182200746	1.58941158877306E-4
58-59	1.1056776269725634E-4	0.0012162453896698198	0.0	0.5016528498426206	1.658516440458845E-4
60-61	1.2438873303441338E-4	0.0012507978155127124	0.0	0.8903538196048253	1.658516440458845E-4
62-63	1.2438873303441338E-4	0.0012646187858498695	0.0	0.9004984118322985	1.934935847201986E-4
64-65	1.3820970337157044E-4	0.0015064857667501177	0.0	0.9114446203393269	1.934935847201986E-4
66-67	1.5203067370872747E-4	0.0015341277074244317	0.0	0.9204489825139848	1.934935847201986E-4
68-69	1.5203067370872747E-4	0.0015617696480987459	0.0	0.9296883011843742	2.004040698887771E-4
70-71	1.5203067370872747E-4	0.001575590618435903	0.0	0.9384507963781319	2.211355253945127E-4
72-73	1.5203067370872747E-4	0.001575590618435903	0.0	0.9455271331907562	2.3495649573166972E-4
74-75	1.5203067370872747E-4	0.0016239640146159524	0.0	0.95197461585304	2.3495649573166972E-4
76-77	1.5203067370872747E-4	0.0016930688663017379	0.0	0.958055842801389	2.3495649573166972E-4
78-79	1.58941158877306E-4	0.0017276212921446305	0.0	0.9638260979171521	2.3495649573166972E-4
80-81	1.658516440458845E-4	0.001734531777313209	0.0	0.9677305220373991	2.4186698090024824E-4
82-83	1.658516440458845E-4	0.0017414422624817873	0.0	0.9709991815221366	2.4877746606882675E-4
84-85	1.658516440458845E-4	0.0017414422624817873	0.0	0.9739637796594568	2.4877746606882675E-4
86-87	1.658516440458845E-4	0.0017552632328189444	0.0	0.97433694585856	2.4877746606882675E-4
88-89	1.658516440458845E-4	0.0018174575993361512	0.0	0.9743922297399087	2.4877746606882675E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7475	0.0	48.535816	1
GTATCAA	13105	0.0	33.36912	1
TCAACGC	16900	0.0	25.20293	4
ATCAACG	17030	0.0	24.961115	3
CAACGCA	17305	0.0	24.613092	5
ACCGAGG	6090	0.0	24.380709	92-93
GACCGAG	6010	0.0	24.35946	90-91
GAGACCG	5945	0.0	24.179989	88-89
CGAGACC	6185	0.0	23.785675	88-89
TATCAAC	18075	0.0	23.70477	2
AACGCAG	17950	0.0	23.68177	6
AGACCGA	6210	0.0	23.47288	90-91
ACGAGAC	6570	0.0	22.615482	86-87
CACGAGA	6815	0.0	21.583992	86-87
GCCCACG	7095	0.0	20.761198	82-83
ACGCAGA	20910	0.0	20.369658	7
GTACATG	20110	0.0	20.322327	1
TACATGG	19840	0.0	20.23825	2
CCCACGA	7210	0.0	20.205563	84-85
CCACGAG	7420	0.0	20.025597	84-85
>>END_MODULE