FastQCFastQC Report
Fri 27 May 2016
SRR2139185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000332
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97450.048.2891731
GTATCAA177200.034.0850451
ATCTCGT100800.027.06126694
ATCAACG221800.026.361113
TCAACGC227050.025.848074
CAACGCA231150.025.4810565
TATCAAC240200.024.8415742
AACGCAG236500.024.6214566
TATCTCG115000.021.27304694
ACGCAGA278650.020.8366077
CGCAGAG278750.020.5419398
GTACATG244100.020.3324891
TACATGG241500.019.894172
ACATGGG247100.018.9747833
GCCGTCT59900.018.31176890-91
AGAGTAC300350.017.60175710-11
GCAGAGT321800.017.2439429
CATGGGG175350.016.2288954
CAGAGTA324650.016.1710979
ATGCCGT66250.016.14262888-89
Produced by FastQC (version 0.11.5)