##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10000332
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.87755026533119	31.0	30.0	31.0	26.0	34.0
2	30.38427394210512	31.0	30.0	31.0	27.0	34.0
3	30.007987934800564	31.0	30.0	31.0	26.0	34.0
4	33.87241223591377	35.0	35.0	37.0	30.0	37.0
5	33.635599898083385	35.0	33.0	37.0	28.0	37.0
6	33.629826389763856	35.0	33.0	37.0	28.0	37.0
7	33.59235353386268	35.0	33.0	37.0	28.0	37.0
8	33.49689460309918	35.0	33.0	37.0	28.0	37.0
9	34.762505684811266	37.0	33.0	39.0	28.0	39.0
10-11	34.759204344415764	37.0	33.0	39.0	28.0	39.0
12-13	34.64089717221388	37.0	33.0	39.0	27.0	39.0
14-15	35.459137256643075	38.0	33.0	40.0	27.0	41.0
16-17	35.35260774342292	37.0	33.0	40.0	27.0	41.0
18-19	35.520616965516744	37.5	33.5	40.0	27.0	41.0
20-21	35.52515419837499	38.0	34.0	40.0	27.0	41.0
22-23	35.47470686794653	38.0	33.5	40.0	27.0	41.0
24-25	35.46652508746861	38.0	33.5	40.0	27.0	41.0
26-27	35.15794039869917	38.0	33.0	40.0	27.0	41.0
28-29	34.898155421640354	37.0	33.0	40.0	26.0	41.0
30-31	34.8500605161755	37.0	33.0	40.0	25.5	41.0
32-33	34.810318038673316	37.0	33.0	40.0	25.5	41.0
34-35	34.57786598184228	37.0	33.0	40.0	25.0	41.0
36-37	34.47578459659843	37.0	32.5	40.0	25.0	41.0
38-39	34.43214109245134	37.0	32.5	40.0	25.0	41.0
40-41	34.35181789268956	37.0	32.0	40.0	24.0	41.0
42-43	34.33930827599143	37.0	32.0	40.0	24.5	41.0
44-45	34.41282826017423	37.0	32.0	40.0	24.5	41.0
46-47	34.2330457561273	37.0	32.0	40.0	24.0	41.0
48-49	34.20830384395144	37.0	32.0	40.0	24.0	41.0
50-51	34.14790719756422	37.0	32.0	40.0	24.0	41.0
52-53	34.2102215730415	37.0	32.0	40.0	24.0	41.0
54-55	34.30230712850664	37.0	32.0	40.0	24.5	41.0
56-57	34.25510883296704	36.5	32.0	39.5	25.0	41.0
58-59	33.97015617923773	36.0	31.0	39.0	24.0	40.0
60-61	33.63133443216344	36.0	31.0	39.0	23.5	40.0
62-63	33.541164196167244	36.0	31.0	39.0	23.5	40.0
64-65	33.38211242258045	35.0	31.0	39.0	23.5	40.0
66-67	33.13145383778553	35.0	31.0	38.5	23.0	40.0
68-69	32.95964241110076	35.0	30.5	38.0	23.0	40.0
70-71	32.95269568356333	35.0	31.0	38.0	24.0	40.0
72-73	32.74214912773547	34.0	30.0	37.5	24.0	40.0
74-75	32.35805694917204	34.0	30.0	37.0	23.0	39.0
76-77	31.444344548499238	33.5	28.5	36.0	22.5	39.0
78-79	31.832761282995264	34.0	30.0	36.0	23.5	39.0
80-81	31.83232543406239	34.0	30.0	36.0	24.0	38.0
82-83	31.853066239428475	34.0	30.0	35.0	25.0	37.0
84-85	31.604286097460367	34.0	30.0	35.0	24.5	37.0
86-87	31.419059709426143	34.0	30.0	35.0	24.0	36.0
88-89	31.385778675919084	34.0	30.0	35.0	25.0	36.0
90-91	31.278454923742032	33.0	30.0	35.0	24.5	36.0
92-93	31.21736483278578	33.0	30.0	35.0	25.0	35.5
94-95	31.406888390559736	33.0	30.0	35.0	25.0	35.0
96-97	31.644963697276367	34.0	30.0	35.0	25.5	35.0
98-99	32.12870679517383	34.0	31.0	35.0	26.5	35.0
100	31.708945417630364	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	11.0
19	125.0
20	1022.0
21	5328.0
22	19115.0
23	51850.0
24	108392.0
25	187535.0
26	281337.0
27	379380.0
28	473510.0
29	557031.0
30	630296.0
31	699519.0
32	764868.0
33	829461.0
34	895665.0
35	950480.0
36	999607.0
37	1019864.0
38	835134.0
39	302468.0
40	8332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.47215912431707	22.949318082639657	15.234154226079694	25.344368566963578
2	14.17745001493256	23.595374247145962	39.45849318573204	22.768682552189446
3	16.622452134852104	28.150682961839475	31.901092090637235	23.32577281267119
4	12.077348825271041	20.609422655392354	39.24308902039844	28.07013949893816
5	12.011836011778813	39.612090723424274	36.23994132992438	12.136131934872536
6	27.57454140194584	40.798454848672264	19.17788589659426	12.449117852787637
7	24.040051870277907	34.045739681442576	24.80772638348407	17.106482064795447
8	23.364944283849777	37.658819727185055	21.751387853923248	17.224848135041917
9	24.47576584409403	16.758310148667572	22.323920143999803	36.44200386323859
10-11	22.967574325753816	27.74934775885065	30.108950329547362	19.174127585848172
12-13	23.64507110788866	25.261184259309307	30.060788618537803	21.03295601426423
14-15	20.965974977929484	26.738213628218226	27.378897389551536	24.916914004300757
16-17	20.488489001985187	29.612170201425904	28.202621364150325	21.696719432438588
18-19	20.6017848428544	29.029907579893617	29.716646916129474	20.65166066112251
20-21	21.32610693147356	28.10149141450039	29.496194309546166	21.076207344479887
22-23	20.996679592342208	28.158770845346986	29.435961926445763	21.408587635865047
24-25	20.939161077763018	28.260783376937926	29.43008300558136	21.36997253971769
26-27	21.088056208647764	28.330400620633476	29.237398970766442	21.344144199952318
28-29	21.012601079665245	28.27363517327695	29.353205067692745	21.360558679365056
30-31	21.280899847008268	28.23247261175494	29.26843886739156	21.21818867384523
32-33	21.155560373023246	28.405188244650613	29.17434513598913	21.26490624633701
34-35	21.207204244219728	28.269741718888753	29.280628031178924	21.242426005712595
36-37	21.151665022941614	28.277895231872947	29.275028590959185	21.295411154226258
38-39	21.17934681716043	28.24997227102618	29.199683882042045	21.370997029771345
40-41	21.384303399578634	28.136774622207604	29.18027808525217	21.29864389296159
42-43	21.104056833408197	28.259090586558788	29.09855296129039	21.538299618742624
44-45	21.218767437978343	28.44623350591909	28.830175295460187	21.504823760642385
46-47	21.163972285889525	28.241891625061715	28.781790724011096	21.812345365037658
48-49	21.151292703372373	28.39906812177882	28.718381853231822	21.731257321616983
50-51	21.01442374181638	28.460729418341806	28.625189226840646	21.899657613001168
52-53	21.00789754090357	28.42700064927786	28.55185354517216	22.013248264646407
54-55	21.051073603186747	28.24670554077923	28.854631862783663	21.847588993250355
56-57	21.116176926341907	28.013463055233423	28.80898227384488	22.061377744579787
58-59	20.85287841875853	27.934511700048443	28.9486023074443	22.26400757374873
60-61	21.047947495621084	28.09673587811031	29.00217936475394	21.85313726151467
62-63	20.72400478799768	28.366605627720677	28.870349081036185	22.03904050324546
64-65	20.47362767285142	28.57001829208709	28.79355791400457	22.162796121056914
66-67	20.612992741336917	29.021406991658587	28.35469081706296	22.010909449941536
68-69	20.63966107825594	29.01382583804018	28.188646932993073	22.157866150710806
70-71	20.742341593992347	28.533178850007378	28.120608476260443	22.60387107973983
72-73	20.93646703212616	28.296317218652494	28.06145789328799	22.705757855933356
74-75	20.976257749567736	28.232504797166936	28.298521730981413	22.492715722283915
76-77	21.23767230320756	28.191251986767725	27.88404346338073	22.687032246643987
78-79	21.392029313630566	28.245747081583144	27.5223306594217	22.839892945364586
80-81	21.353502291994207	28.490572756278077	27.670990026627628	22.484934925100085
82-83	21.29283726114272	28.350398709404306	27.741077600973195	22.615686428479776
84-85	21.336066559359082	28.713408136259076	27.73782948627317	22.212695818108674
86-87	21.534104091617905	28.695406803862806	27.81709264634849	21.953396458170793
88-89	21.448906142341386	28.703597260287395	28.042305139816655	21.805191457554567
90-91	21.249412852453535	28.44026523118488	28.202965493874967	22.107356422486625
92-93	21.24153662047634	28.086969854584332	28.388846156669867	22.282647368269465
94-95	20.624131938132184	28.49984365796726	28.86127007904362	22.014754324856927
96-97	20.594725564038058	28.537461590965375	28.995574283494786	21.872238561501785
98-99	21.79955237592257	30.419069970005154	29.220026896584127	18.561350757488146
100	21.073039745600255	31.449687834921413	22.85260238983039	24.624670029647937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1569.0
1	1504.5
2	2729.0
3	4200.0
4	5170.0
5	8058.0
6	11069.0
7	13172.0
8	14525.0
9	14383.0
10	13195.5
11	11763.0
12	12081.5
13	14914.5
14	22742.0
15	34828.0
16	45131.5
17	50766.5
18	52899.5
19	53457.0
20	53249.5
21	53538.0
22	55882.0
23	62569.0
24	74557.5
25	91971.5
26	114437.5
27	138719.5
28	162786.0
29	189536.5
30	214389.0
31	235792.0
32	259238.0
33	280388.0
34	301619.5
35	323977.5
36	345312.0
37	364870.5
38	377331.0
39	384213.0
40	391856.5
41	398482.0
42	402428.0
43	406635.0
44	409698.0
45	412756.5
46	414222.0
47	411199.5
48	404553.5
49	391672.0
50	376437.0
51	360138.0
52	339396.0
53	316406.0
54	291836.0
55	265656.0
56	239626.5
57	212875.5
58	186373.0
59	160353.5
60	131684.5
61	104444.5
62	82241.5
63	63502.0
64	47113.0
65	34854.5
66	26206.5
67	20134.0
68	15827.0
69	12066.0
70	9070.0
71	6898.0
72	5156.0
73	3701.5
74	2640.5
75	1900.0
76	1355.5
77	962.5
78	705.5
79	531.0
80	400.0
81	296.5
82	233.0
83	171.0
84	119.0
85	88.0
86	67.0
87	50.0
88	31.0
89	19.5
90	15.0
91	11.0
92	8.0
93	4.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02045932075055108
3	1.5929371144877988
4	0.4211860166242481
5	3.9998672044088137E-5
6	1.6999435618737457E-4
7	0.0
8	0.0
9	0.019849341001878736
10-11	0.12844573560157804
12-13	0.0035148833058742447
14-15	4.999834005511017E-6
16-17	4.499850604959915E-5
18-19	0.06510783841976446
20-21	0.023918333019706296
22-23	6.553426131181381E-4
24-25	1.1513522055978645E-4
26-27	0.003808898157430481
28-29	0.0011341841755512347
30-31	8.24610935738824E-4
32-33	0.0020871523221409707
34-35	0.02573293983737814
36-37	0.0
38-39	3.0625640490608466E-5
40-41	0.0013752130874719166
42-43	8.77925084999932E-5
44-45	5.618095886851549E-4
46-47	0.0013009573997302822
48-49	0.004659174016764882
50-51	0.00703963554618406
52-53	0.004702829840915947
54-55	0.017132255702108867
56-57	0.006415283012470513
58-59	0.007349625236615
60-61	0.008444114209967856
62-63	0.007023181345813407
64-65	0.021126645635080727
66-67	0.0031350279663101776
68-69	0.002485623382916282
70-71	0.001813577869297471
72-73	6.641040868554113E-4
74-75	1.7288132299280462E-4
76-77	7.862093556978044E-5
78-79	0.0
80-81	0.0
82-83	1.2685730994634252E-5
84-85	3.87252049773506E-5
86-87	4.602115553073043E-5
88-89	1.3421935106151734E-5
90-91	2.7501048477473205E-5
92-93	0.0022236260479367186
94-95	7.998888299960638E-5
96-97	1.5889063466821137E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1050.0
20-21	3279.0
22-23	5649.0
24-25	10722.0
26-27	12661.0
28-29	16795.0
30-31	25234.0
32-33	30918.0
34-35	42447.0
36-37	49053.0
38-39	42731.0
40-41	61964.0
42-43	67242.0
44-45	78314.0
46-47	79216.0
48-49	73231.0
50-51	47626.0
52-53	26688.0
54-55	27659.0
56-57	30748.0
58-59	63377.0
60-61	102229.0
62-63	98590.0
64-65	107035.0
66-67	115852.0
68-69	119793.0
70-71	122762.0
72-73	120253.0
74-75	122055.0
76-77	114349.0
78-79	129768.0
80-81	134438.0
82-83	135818.0
84-85	139959.0
86-87	148470.0
88-89	174974.0
90-91	185197.0
92-93	200852.0
94-95	238377.0
96-97	469847.0
98-99	832853.0
100-101	5390257.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.56044308343743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.53309906667272	81.94772253228147
2	5.093621345810365	9.021867265684417
3	1.3033805321346925	3.4628387229652455
4	0.5017892690575956	1.7775472000901948
5	0.22751030537551312	1.0074206725051797
6	0.11694569116199463	0.6214057335603046
7	0.06943125741562452	0.4304204044397536
8	0.04360043536307311	0.3089019099507624
9	0.027521874696520756	0.2193614475849651
>10	0.08163898787921572	1.0730705244353553
>50	0.0011749606178272853	0.071004509061077
>100	2.537408409696396E-4	0.042592975404678365
>500	3.2532973899389234E-5	0.015846102036570185
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.999668011022034E-6
2	0.0	1.9999336022044068E-5	0.0	9.999668011022034E-6	9.999668011022034E-6
3	0.0	1.9999336022044068E-5	0.0	1.9999336022044068E-5	9.999668011022034E-6
4	0.0	7.999734408817627E-5	0.0	4.999834005511017E-5	9.999668011022034E-6
5	0.0	7.999734408817627E-5	0.0	4.999834005511017E-5	9.999668011022034E-6
6	0.0	7.999734408817627E-5	0.0	4.999834005511017E-5	2.9999004033066102E-5
7	0.0	7.999734408817627E-5	0.0	5.9998008066132205E-5	2.9999004033066102E-5
8	0.0	8.999701209919831E-5	0.0	6.999767607715424E-5	2.9999004033066102E-5
9	0.0	1.6999435618737457E-4	0.0	9.999668011022033E-5	4.999834005511017E-5
10-11	0.0	1.6999435618737457E-4	0.0	1.2999568414328645E-4	4.999834005511017E-5
12-13	0.0	1.6999435618737457E-4	0.0	1.449951861598195E-4	4.999834005511017E-5
14-15	0.0	2.299923642535068E-4	0.0	1.799940241983966E-4	9.499684610470932E-5
16-17	0.0	2.299923642535068E-4	0.0	1.9999336022044067E-4	1.0999634812124237E-4
18-19	9.999668011022034E-6	2.649912022920839E-4	0.0	1.9999336022044067E-4	1.2999568414328645E-4
20-21	9.999668011022034E-6	3.04989874336172E-4	0.0	1.9999336022044067E-4	1.2999568414328645E-4
22-23	9.999668011022034E-6	3.4498854638026017E-4	0.0	2.9499020632515E-4	1.499950201653305E-4
24-25	9.999668011022034E-6	4.049865544463924E-4	0.0	4.4998506049599154E-4	1.6999435618737457E-4
26-27	9.999668011022034E-6	4.3498555847945846E-4	9.999668011022034E-6	8.199727769038068E-4	1.6999435618737457E-4
28-29	9.999668011022034E-6	4.4998506049599154E-4	9.999668011022034E-6	0.0037948740101828617	1.7999402419839662E-4
30-31	9.999668011022034E-6	4.699843965180356E-4	9.999668011022034E-6	0.014899505336422831	1.7999402419839662E-4
32-33	9.999668011022034E-6	4.699843965180356E-4	9.999668011022034E-6	0.038088735453982926	1.7999402419839662E-4
34-35	9.999668011022034E-6	5.499817406062118E-4	9.999668011022034E-6	0.06638779592517528	2.049931942259517E-4
36-37	1.9999336022044068E-5	6.299790846943881E-4	9.999668011022034E-6	0.11905104750522283	2.0999302823146272E-4
38-39	1.9999336022044068E-5	6.599780887274543E-4	9.999668011022034E-6	0.28260061765949374	2.1999269624248474E-4
40-41	1.9999336022044068E-5	6.899770927605203E-4	9.999668011022034E-6	0.4655595434231583	2.299923642535068E-4
42-43	1.9999336022044068E-5	7.049765947770534E-4	9.999668011022034E-6	0.5776958204987594	2.299923642535068E-4
44-45	2.9999004033066102E-5	7.599747688376746E-4	9.999668011022034E-6	0.7253259191794832	2.299923642535068E-4
46-47	2.9999004033066102E-5	8.149729428982958E-4	9.999668011022034E-6	0.8789208198287817	2.3499219825901782E-4
48-49	2.9999004033066102E-5	8.199727769038068E-4	9.999668011022034E-6	1.0231010330457029	2.599913682865729E-4
50-51	3.9998672044088137E-5	8.79970784969939E-4	9.999668011022034E-6	1.171721098859518	2.599913682865729E-4
52-53	3.9998672044088137E-5	8.89970452980961E-4	9.999668011022034E-6	1.61887625330839	2.79990704308617E-4
54-55	3.9998672044088137E-5	9.049699549974941E-4	9.999668011022034E-6	2.180527606483465	3.04989874336172E-4
56-57	3.9998672044088137E-5	9.099697890030051E-4	9.999668011022034E-6	2.7854925216482815	3.0998970834168304E-4
58-59	3.9998672044088137E-5	9.099697890030051E-4	9.999668011022034E-6	3.4228013629947487	3.199893763527051E-4
60-61	3.9998672044088137E-5	9.099697890030051E-4	9.999668011022034E-6	3.6299894843491196	3.2998904436372714E-4
62-63	3.9998672044088137E-5	9.349689590305601E-4	9.999668011022034E-6	3.6566685986025265	3.3998871237474914E-4
64-65	3.9998672044088137E-5	0.0010449653071518026	9.999668011022034E-6	3.675237982098994	3.3998871237474914E-4
66-67	3.9998672044088137E-5	0.0010749643111848685	9.999668011022034E-6	3.6823927445608806	3.5498821439128216E-4
68-69	3.9998672044088137E-5	0.0011149629832289567	9.999668011022034E-6	3.689667503038899	3.6998771640781524E-4
70-71	3.9998672044088137E-5	0.0011249626512399788	9.999668011022034E-6	3.6955623073313966	3.7498755041332626E-4
72-73	3.9998672044088137E-5	0.0011299624852454898	9.999668011022034E-6	3.7012071199236187	3.9998672044088134E-4
74-75	3.9998672044088137E-5	0.001144961987262023	9.999668011022034E-6	3.7054669784963137	3.9998672044088134E-4
76-77	3.9998672044088137E-5	0.0011899604933116221	9.999668011022034E-6	3.709381848522629	4.0498655444639236E-4
78-79	3.9998672044088137E-5	0.0012099598293336662	9.999668011022034E-6	3.7116867719991697	4.099863884519034E-4
80-81	3.9998672044088137E-5	0.0012099598293336662	9.999668011022034E-6	3.7126717392982553	4.2998572447394744E-4
82-83	3.9998672044088137E-5	0.001219959497344688	9.999668011022034E-6	3.7134467135691094	4.4998506049599154E-4
84-85	3.9998672044088137E-5	0.001219959497344688	9.999668011022034E-6	3.7137967019494953	4.4998506049599154E-4
86-87	3.9998672044088137E-5	0.0012299591653557101	9.999668011022034E-6	3.7139966953097154	4.4998506049599154E-4
88	3.9998672044088137E-5	0.0012299591653557101	9.999668011022034E-6	3.714016694645738	4.4998506049599154E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9745	0.0	48.289173	1
GTATCAA	17720	0.0	34.085045	1
ATCTCGT	10080	0.0	27.061266	94
ATCAACG	22180	0.0	26.36111	3
TCAACGC	22705	0.0	25.84807	4
CAACGCA	23115	0.0	25.481056	5
TATCAAC	24020	0.0	24.841574	2
AACGCAG	23650	0.0	24.621456	6
TATCTCG	11500	0.0	21.273046	94
ACGCAGA	27865	0.0	20.836607	7
CGCAGAG	27875	0.0	20.541939	8
GTACATG	24410	0.0	20.332489	1
TACATGG	24150	0.0	19.89417	2
ACATGGG	24710	0.0	18.974783	3
GCCGTCT	5990	0.0	18.311768	90-91
AGAGTAC	30035	0.0	17.601757	10-11
GCAGAGT	32180	0.0	17.243942	9
CATGGGG	17535	0.0	16.228895	4
CAGAGTA	32465	0.0	16.171097	9
ATGCCGT	6625	0.0	16.142628	88-89
>>END_MODULE