FastQCFastQC Report
Fri 27 May 2016
SRR2139184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14697226
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99350.037.5041281
GTATCAA170300.031.7952021
TCAACGC178150.028.529834
ATCAACG184950.027.5270583
CAACGCA185350.027.3512635
AACGCAG192900.026.278346
TATCAAC207500.025.1030752
GTACATG448750.022.8968281
TACATGG454250.022.064742
ACGCAGA231550.021.8366157
ACATGGG455750.021.5030253
CATGGGG345700.019.2831174
CGCAGAG277650.018.2092698
TATATAC174300.017.1590843
ATACAAC127700.016.3780166
TATACAA155500.016.2603115
GCAGAGT322650.015.6433819
AGAGTAC266650.015.39334410-11
ATGGGGG186450.014.9352975
CATATAT172000.014.3117421
Produced by FastQC (version 0.11.5)