##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139184_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14697226
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.515698200463135	31.0	31.0	34.0	30.0	34.0
2	31.507001661401954	31.0	31.0	34.0	28.0	34.0
3	31.75060184826715	33.0	31.0	34.0	30.0	34.0
4	35.28076876547996	37.0	35.0	37.0	33.0	37.0
5	35.36691522604334	37.0	35.0	37.0	33.0	37.0
6	35.307040185678574	37.0	35.0	37.0	32.0	37.0
7	35.30065435477416	37.0	35.0	37.0	32.0	37.0
8	35.29858226307468	37.0	35.0	37.0	32.0	37.0
9	36.983880427503806	39.0	37.0	39.0	33.0	39.0
10-11	37.001827147517496	39.0	37.0	39.0	33.0	39.0
12-13	36.95291638707876	39.0	37.0	39.0	33.0	39.0
14-15	38.30536214793186	40.0	38.0	41.0	33.0	41.0
16-17	38.29445355878721	40.0	38.0	41.0	33.0	41.0
18-19	38.28105664293385	40.0	38.0	41.0	33.0	41.0
20-21	38.346974752168876	40.0	38.0	41.0	33.0	41.0
22-23	38.308216501865	40.0	38.0	41.0	33.0	41.0
24-25	38.33849731273922	40.0	38.0	41.0	33.0	41.0
26-27	38.33508753431759	40.0	38.0	41.0	33.0	41.0
28-29	38.267427745513885	40.0	38.0	41.0	33.0	41.0
30-31	38.19639721656662	40.0	38.0	41.0	33.0	41.0
32-33	38.16990913548655	40.0	38.0	41.0	33.0	41.0
34-35	38.08870220375969	40.0	38.0	41.0	32.5	41.0
36-37	37.98927898150032	40.0	38.0	41.0	32.0	41.0
38-39	37.89274895319266	40.0	37.0	41.0	32.0	41.0
40-41	37.82815932411867	40.0	37.0	41.0	32.0	41.0
42-43	37.63449383883661	40.0	37.0	41.0	31.5	41.0
44-45	37.377683380130755	39.5	36.0	41.0	31.0	41.0
46-47	37.212109185760795	39.0	36.0	40.5	31.0	41.0
48-49	37.05835393744452	39.0	36.0	40.0	30.5	41.0
50-51	36.90046217186651	39.0	35.0	40.0	30.0	41.0
52-53	36.73876380320121	39.0	35.0	40.0	30.0	41.0
54-55	36.542910226753946	38.0	35.0	40.0	30.0	41.0
56-57	36.504008930402705	38.0	35.0	40.0	30.0	41.0
58-59	36.54577642636309	38.0	35.0	40.0	30.0	41.0
60-61	36.85289856812859	39.0	35.0	40.0	31.0	41.0
62-63	36.80586041267371	38.5	35.0	40.0	31.0	41.0
64-65	36.57312992164208	38.0	35.0	40.0	31.0	41.0
66-67	36.3320007774056	37.5	35.0	40.0	31.0	41.0
68-69	36.03994916312813	37.0	34.5	40.0	30.0	41.0
70-71	35.78641699000993	36.5	34.0	39.0	30.0	41.0
72-73	35.4882506089404	36.0	34.0	39.0	30.0	41.0
74-75	35.18703076085751	35.5	34.0	38.5	30.0	40.0
76-77	34.8978015612424	35.0	34.0	37.5	30.0	39.5
78-79	34.61448851016942	35.0	34.0	37.0	30.0	39.0
80-81	34.341442942661324	35.0	34.0	36.5	30.0	39.0
82-83	34.11733430582367	35.0	34.0	36.0	29.5	38.0
84-85	33.91218671218821	35.0	34.0	36.0	29.0	37.0
86-87	33.74393712456734	35.0	33.0	35.5	29.0	37.0
88-89	33.615109221421505	35.0	33.0	35.0	29.0	36.5
90-91	33.507477022527844	35.0	33.0	35.0	29.0	36.0
92-93	33.44012085806068	35.0	33.0	35.0	29.0	36.0
94-95	33.426576988389094	35.0	33.0	35.0	29.0	36.0
96-97	33.44764264770011	35.0	33.0	35.0	30.0	35.5
98-99	33.49525752435835	35.0	33.0	35.0	30.0	35.0
100	32.75862916608953	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	4.0
16	96.0
17	528.0
18	2255.0
19	7206.0
20	18904.0
21	37554.0
22	60117.0
23	83914.0
24	108802.0
25	134288.0
26	155852.0
27	167813.0
28	191217.0
29	228840.0
30	269114.0
31	321677.0
32	404302.0
33	530009.0
34	726512.0
35	1050839.0
36	1697882.0
37	2903336.0
38	3531717.0
39	1981056.0
40	83390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80046629207443	19.983798303162786	13.349872962421616	24.86586244234116
2	14.66757043699305	21.089255099903546	41.11782307940526	23.12535138369814
3	17.91947399432666	26.28442003415547	30.049518217861376	25.746587753656495
4	12.384205313099823	17.84334681803009	38.91740648755804	30.85504138131204
5	13.47070417999998	37.78561350756544	35.6441356848144	13.099546627620182
6	30.052861503758404	37.73541456719895	19.14232769858824	13.069396230454409
7	26.590508040759325	32.163261878593204	23.84074575859192	17.405484322055553
8	24.59502487318459	36.504711997426405	21.36074200963574	17.539521119753264
9	25.735144479432936	16.627138764335797	21.172660422081382	36.46505633414988
10-11	24.288978509027302	26.69872715702819	29.07391484136368	19.938379492580825
12-13	24.84186249609396	24.389998287040036	28.723361729004292	22.044777487861715
14-15	22.27257764612451	25.559304117412363	25.948905750159625	26.219212486303505
16-17	22.393028580371023	28.49551443647238	26.544564762556007	22.566892220600593
18-19	22.533634415154484	28.20083426644612	27.360325144416738	21.905206173982663
20-21	23.458866835028942	27.386597569849574	27.017819031236908	22.136716563884573
22-23	23.251882600023503	27.292684048531136	26.840383111872683	22.61505023957267
24-25	22.94789934430365	27.438946944217957	26.979342681992197	22.6338110294862
26-27	22.815799747194923	27.46039240937587	26.964472971119115	22.759334872310088
28-29	22.925721615605184	27.428405154426233	26.955092215766907	22.690781014201676
30-31	23.187554847796026	27.175688460237758	26.882289031296956	22.75446766066926
32-33	22.926579735690563	27.337690550706927	26.901739874109058	22.833989839493444
34-35	23.10175817552394	27.366620528422747	26.895955302018255	22.635665994035055
36-37	23.13616113621348	27.165130774585638	26.82998424526046	22.868723843940423
38-39	22.85585202705071	27.422026849325643	26.990210984471325	22.73191013915232
40-41	23.003089045553335	27.138126470190972	27.05051054327558	22.808273940980108
42-43	22.776202535133013	27.324299613564673	26.904538411911695	22.99495943939062
44-45	22.750830096479138	27.261685423334963	26.99787706029092	22.989607419894977
46-47	22.824969299697035	27.227304601543047	26.98052812886649	22.967197969893434
48-49	22.898192619575934	27.36056597661477	26.89676863780475	22.844472766004547
50-51	22.812698373252534	27.36421447937905	26.95263965933796	22.87044748803045
52-53	23.036591372301704	27.23101492659567	26.866113834705054	22.86627986639757
54-55	23.030775275568264	27.174330259179825	26.893546598069086	22.901347867182825
56-57	23.00688467447663	27.36086227160099	26.780009857235253	22.852243196687127
58-59	22.892293312592958	27.277897550422008	26.943864532591043	22.885944604393988
60-61	22.941440504220694	27.267474277804116	26.85182679127988	22.939258426695307
62-63	22.93252031316468	27.338573828194757	26.975787586869533	22.75311827177103
64-65	23.053790208220953	27.188003827040774	26.907710217571886	22.850495747166388
66-67	23.05564136280053	27.26717930084651	26.74649304803509	22.930686288317865
68-69	22.96931317944505	27.250859046218977	26.8313794821615	22.948448292174476
70-71	23.000258074031866	27.07666589974665	26.88216433300692	23.040911693214568
72-73	22.95223379730976	27.170130338675563	26.7635895841023	23.11404627991238
74-75	22.821432319304304	27.203788055788756	26.875300301489496	23.099479323417444
76-77	22.928282240139325	27.268418391606048	26.7965270835286	23.006772284726026
78-79	22.763910070827336	27.27763079675532	26.871292105631344	23.087167026786
80-81	22.836207703000447	27.259551912096114	26.902941096907107	23.001299287996332
82-83	22.982966428324765	27.16814247743664	27.03294990623057	22.81594118800802
84-85	22.888999492387743	27.206874064867172	26.9758036405853	22.928322802159787
86-87	22.740173354184115	27.39154192164927	26.97362343084957	22.894661293317046
88-89	22.92590653643615	27.18967878716661	26.854029406802997	23.03038526959424
90-91	22.918963390375875	27.283729687429414	26.889673833051837	22.907633089142877
92-93	22.937704314790917	27.330149587955297	26.97280918723423	22.759336910019552
94-95	22.63901732334609	27.470645251215135	27.04288156944696	22.847455855991818
96-97	22.734238426807483	27.5352692088499	27.093580275759077	22.636912088583543
98-99	23.89814726711705	29.540962659888265	27.183430907043398	19.37745916595129
100	22.803266438281838	30.508320930384386	20.90688348227165	25.781529149062127
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4863.0
1	3728.0
2	2823.5
3	2982.0
4	2900.0
5	3036.0
6	3499.5
7	4085.0
8	4532.5
9	5047.0
10	5675.5
11	6243.0
12	6859.0
13	7703.5
14	8861.0
15	10340.5
16	12342.5
17	14840.5
18	18001.0
19	22163.5
20	27655.5
21	35085.0
22	45306.0
23	59118.5
24	76545.5
25	98759.5
26	124806.0
27	151374.0
28	178694.0
29	208813.0
30	236963.0
31	262850.5
32	291469.5
33	321153.5
34	349996.0
35	378785.0
36	411267.0
37	440564.5
38	464620.0
39	488498.0
40	510721.5
41	529540.0
42	542660.5
43	556127.5
44	572360.5
45	596165.5
46	618538.0
47	640646.5
48	653602.5
49	650982.5
50	648609.0
51	644559.0
52	619368.5
53	579918.5
54	551980.5
55	526877.0
56	498156.5
57	460039.0
58	418281.0
59	372491.5
60	316224.0
61	267911.5
62	223526.0
63	181829.0
64	149891.0
65	118109.0
66	91546.0
67	68564.0
68	48676.5
69	37565.5
70	29539.5
71	21973.5
72	16830.0
73	12729.5
74	9527.0
75	7071.0
76	5171.0
77	3947.0
78	3101.0
79	2449.0
80	1945.0
81	1526.0
82	1202.0
83	813.0
84	527.0
85	397.0
86	310.0
87	232.5
88	140.5
89	74.5
90	43.5
91	29.5
92	24.0
93	19.5
94	13.5
95	10.0
96	9.5
97	10.0
98	5.5
99	10.5
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0119206168565415
3	0.022044976378535648
4	0.018989978108794135
5	0.010750327987063681
6	0.01383254227702561
7	0.015533543540801508
8	0.001864297385098385
9	8.845206571634674E-5
10-11	5.7834042968380565E-5
12-13	0.0030175762419384447
14-15	2.3133617187352226E-4
16-17	5.171043841878732E-4
18-19	2.0412015165310787E-5
20-21	7.82910866687095E-5
22-23	1.0219949320633979E-5
24-25	2.386792391069523E-5
26-27	0.0025292838849800474
28-29	0.0035888379756205792
30-31	0.0026916968446635105
32-33	0.004031457455658604
34-35	0.005937368924741454
36-37	0.0011666707575151685
38-39	0.0015225530699460531
40-41	6.174544695739838E-4
42-43	6.004658484695468E-5
44-45	8.898968057866074E-5
46-47	0.00439082208324295
48-49	7.497253451824225E-4
50-51	2.777446230285522E-4
52-53	0.004323862219706461
54-55	2.2311814901778557E-4
56-57	1.801776236057912E-4
58-59	5.912791779218174E-4
60-61	4.976665563633407E-5
62-63	0.0
64-65	0.0
66-67	0.0018384110826047807
68-69	0.0
70-71	3.7115402234339146E-4
72-73	0.0015504902037988804
74-75	1.2787547074157666E-4
76-77	2.0856148221475273E-5
78-79	8.441120462512625E-6
80-81	0.0030469496913991235
82-83	9.519071004438093E-5
84-85	3.284230007676778E-4
86-87	0.013456979154625123
88-89	6.061777323887856E-4
90-91	8.424791273519228E-4
92-93	4.62283128538055E-5
94-95	2.161343442218527E-4
96-97	7.205439126600863E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	5765.0
20-21	11018.0
22-23	12781.0
24-25	14873.0
26-27	18498.0
28-29	24346.0
30-31	38589.0
32-33	47326.0
34-35	61069.0
36-37	87015.0
38-39	99960.0
40-41	95829.0
42-43	104260.0
44-45	118700.0
46-47	121732.0
48-49	123848.0
50-51	118323.0
52-53	116679.0
54-55	124353.0
56-57	128200.0
58-59	130460.0
60-61	130369.0
62-63	123832.0
64-65	130941.0
66-67	137939.0
68-69	141000.0
70-71	141844.0
72-73	133700.0
74-75	133004.0
76-77	137931.0
78-79	146297.0
80-81	145704.0
82-83	139078.0
84-85	136578.0
86-87	142477.0
88-89	152959.0
90-91	162554.0
92-93	169291.0
94-95	200604.0
96-97	502276.0
98-99	917488.0
100-101	9067736.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.66238952492188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.06724521008974	76.25308197413273
2	6.8825391545208765	11.653844216411459
3	1.6096983852291011	4.088427351237117
4	0.6292030300756469	2.130793280901022
5	0.29963010531529066	1.2683700344798254
6	0.1548144652594883	0.7864177537134772
7	0.08845213910941763	0.5241998618915864
8	0.06531778459742311	0.4423967777993582
9	0.04457750671520986	0.33966344138156257
>10	0.1523827374089691	2.0474623120586704
>50	0.004706185120558231	0.26216546939466406
>100	0.0014030890611511696	0.18046748722951123
>500	3.020749719154573E-5	0.02271003936904511
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.804005055103595E-6
2	0.0	2.0412015165310787E-5	0.0	0.0	6.804005055103595E-6
3	0.0	2.0412015165310787E-5	0.0	6.804005055103595E-6	6.804005055103595E-6
4	0.0	6.123604549593236E-5	0.0	6.804005055103595E-6	6.804005055103595E-6
5	0.0	7.484405560613956E-5	0.0	6.804005055103595E-6	6.804005055103595E-6
6	6.804005055103595E-6	7.484405560613956E-5	0.0	6.804005055103595E-6	6.804005055103595E-6
7	6.804005055103595E-6	7.484405560613956E-5	0.0	1.360801011020719E-5	1.360801011020719E-5
8	6.804005055103595E-6	7.484405560613956E-5	0.0	1.360801011020719E-5	2.721602022041438E-5
9	6.804005055103595E-6	1.360801011020719E-4	0.0	1.360801011020719E-5	3.4020025275517976E-5
10-11	6.804005055103595E-6	1.360801011020719E-4	0.0	1.360801011020719E-5	3.4020025275517976E-5
12-13	6.804005055103595E-6	1.4288410615717552E-4	0.0	1.360801011020719E-5	3.4020025275517976E-5
14-15	1.360801011020719E-5	1.9391414407045248E-4	0.0	2.0412015165310787E-5	5.7834042968380565E-5
16-17	2.0412015165310787E-5	2.0752215418065966E-4	0.0	3.061802274796618E-5	7.824605813369135E-5
18-19	2.0412015165310787E-5	2.2793416934597046E-4	0.0	3.4020025275517976E-5	9.865807329900212E-5
20-21	2.0412015165310787E-5	2.4494418198372944E-4	0.0	5.7834042968380565E-5	1.0886408088165753E-4
22-23	2.0412015165310787E-5	2.993762224245582E-4	0.0	1.496881112122791E-4	1.2247209099186472E-4
24-25	2.0412015165310787E-5	3.742202780306977E-4	0.0	3.9803429572356035E-4	1.530901137398309E-4
26-27	2.3814017692862582E-5	4.1504430836131933E-4	0.0	9.389526976042962E-4	1.632961213224863E-4
28-29	2.721602022041438E-5	4.218483134164229E-4	0.0	0.005235681889902217	1.632961213224863E-4
30-31	2.721602022041438E-5	5.273103917705286E-4	0.0	0.018404833674055227	1.666981238500381E-4
32-33	2.721602022041438E-5	5.37516399353184E-4	0.0	0.04774370347166193	1.7690413143269348E-4
34-35	2.721602022041438E-5	6.701944979277042E-4	0.0	0.0752216778866978	1.7690413143269348E-4
36-37	2.721602022041438E-5	7.858625838644653E-4	0.0	0.11079641831730695	1.7690413143269348E-4
38-39	2.721602022041438E-5	8.300886167226387E-4	0.0	0.14516684985316275	1.871101390153489E-4
40-41	2.721602022041438E-5	8.879226596910192E-4	0.0	0.17289657245523748	2.0071814912555607E-4
42-43	2.721602022041438E-5	9.287466900216408E-4	0.0	0.20057866702192645	2.2113016429086687E-4
44-45	2.721602022041438E-5	0.0010376107709032982	0.0	0.22182417280648742	2.4154217945617765E-4
46-47	2.721602022041438E-5	0.0011158568290369898	0.0	0.23899407956304136	2.653561971490402E-4
48-49	2.721602022041438E-5	0.0011158568290369898	0.0	0.25088748039936243	2.7896420725924745E-4
50-51	6.804005055103597E-5	0.0012383289200288544	0.0	0.2608315337873963	2.7896420725924745E-4
52-53	7.484405560613956E-5	0.0012383289200288544	0.0	0.2691188119445125	3.0277822495211E-4
54-55	7.484405560613956E-5	0.0012655449402492688	0.0	0.27514375842080674	3.231902401174208E-4
56-57	7.484405560613956E-5	0.0013233789832176493	0.0	0.2806924245432437	3.4020025275517977E-4
58-59	7.484405560613956E-5	0.0013471930009105121	0.0	0.2854110020489581	3.5380826286538695E-4
60-61	7.484405560613956E-5	0.001377811023658478	0.0	0.28992886140554686	3.606122679204906E-4
62-63	7.484405560613956E-5	0.001411831048933996	0.0	0.2940282744512468	3.742202780306978E-4
64-65	7.484405560613956E-5	0.0015513131525636199	0.0	0.29749151302429455	3.742202780306978E-4
66-67	7.484405560613956E-5	0.0015581171576187236	0.0	0.300675787390083	3.776222805582496E-4
68-69	7.484405560613956E-5	0.0015955391854217934	0.0	0.30372398165476944	3.8102428308580137E-4
70-71	7.824605813369135E-5	0.001615951200587104	0.0	0.3063095035757088	3.9463229319600855E-4
72-73	9.525607077145034E-5	0.0016363632157524147	0.0	0.3086024532792787	4.218483134164229E-4
74-75	9.525607077145034E-5	0.001666981238500381	0.0	0.3105483987250383	4.218483134164229E-4
76-77	9.865807329900214E-5	0.0017452272966340721	0.0	0.3121711539306805	4.252503159439747E-4
78-79	1.0206007582655393E-4	0.0017826493244371422	0.0	0.31343329686840227	4.286523184715265E-4
80-81	1.0206007582655393E-4	0.0017826493244371422	0.0	0.3141545214042432	4.388583260541819E-4
82-83	1.0546207835410573E-4	0.0017826493244371422	0.0	0.3145219376772188	4.5246633616438913E-4
84-85	1.0886408088165753E-4	0.0017962573345473493	0.0	0.3146750277909587	4.592703412194927E-4
86-87	1.1226608340920933E-4	0.0018234733547677637	0.0	0.31470224381117906	4.626723437470445E-4
88	1.1566808593676113E-4	0.001884709400263696	0.0	0.31471244981876173	4.694763488021481E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9935	0.0	37.504128	1
GTATCAA	17030	0.0	31.795202	1
TCAACGC	17815	0.0	28.52983	4
ATCAACG	18495	0.0	27.527058	3
CAACGCA	18535	0.0	27.351263	5
AACGCAG	19290	0.0	26.27834	6
TATCAAC	20750	0.0	25.103075	2
GTACATG	44875	0.0	22.896828	1
TACATGG	45425	0.0	22.06474	2
ACGCAGA	23155	0.0	21.836615	7
ACATGGG	45575	0.0	21.503025	3
CATGGGG	34570	0.0	19.283117	4
CGCAGAG	27765	0.0	18.209269	8
TATATAC	17430	0.0	17.159084	3
ATACAAC	12770	0.0	16.378016	6
TATACAA	15550	0.0	16.260311	5
GCAGAGT	32265	0.0	15.643381	9
AGAGTAC	26665	0.0	15.393344	10-11
ATGGGGG	18645	0.0	14.935297	5
CATATAT	17200	0.0	14.311742	1
>>END_MODULE