FastQCFastQC Report
Fri 27 May 2016
SRR2139183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12527743
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135250.046.3468931
GTATCAA232900.034.2566341
ATCAACG299300.025.794763
TCAACGC305050.025.267114
CAACGCA308400.025.005825
TATCAAC323900.024.4753932
AACGCAG318400.023.9055756
ACGCAGA366700.020.6883017
CGCAGAG370550.020.224648
AGAGTAC397800.017.61513310-11
GTACATG292550.017.3324341
GCAGAGT428400.016.9850839
CAGAGTA428100.016.6153589
TACATGG292250.016.4957492
ACATGGG290500.016.1584173
GAGTACT276100.016.06649412-13
AGTACTT314100.015.44177312-13
GTGGTAT65200.014.41146851
CATGGGG204900.013.9170794
GTACTTT325700.013.780642514-15
Produced by FastQC (version 0.11.5)