##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12527743
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.830176991976927	31.0	30.0	31.0	26.0	34.0
2	30.350295899269327	31.0	30.0	31.0	27.0	34.0
3	30.161860200995502	31.0	30.0	32.0	27.0	34.0
4	34.01136645283991	35.0	35.0	37.0	30.0	37.0
5	33.730390222724075	35.0	33.0	37.0	28.0	37.0
6	33.728362163879	35.0	33.0	37.0	28.0	37.0
7	33.68043126363624	35.0	33.0	37.0	28.0	37.0
8	33.569596055730074	35.0	33.0	37.0	28.0	37.0
9	34.857113368305846	37.0	33.0	39.0	28.0	39.0
10-11	34.869243526148324	37.0	33.0	39.0	28.0	39.0
12-13	34.75113749539722	37.0	33.0	39.0	27.0	39.0
14-15	35.54854669352652	38.0	33.0	40.0	27.0	41.0
16-17	35.43175007660997	38.0	33.0	40.0	27.0	41.0
18-19	35.623619034969025	38.0	33.5	40.0	27.0	41.0
20-21	35.613431378845235	38.0	34.0	40.0	27.0	41.0
22-23	35.52191306977387	38.0	34.0	40.0	27.0	41.0
24-25	35.526964473023455	38.0	34.0	40.0	27.0	41.0
26-27	35.23799258942013	38.0	33.0	40.0	27.0	41.0
28-29	34.96173722889521	37.5	33.0	40.0	26.0	41.0
30-31	34.9274909072763	37.0	33.0	40.0	26.0	41.0
32-33	34.911806951594144	37.0	33.0	40.0	25.5	41.0
34-35	34.668902341274006	37.0	33.0	40.0	25.0	41.0
36-37	34.544770751702664	37.0	33.0	40.0	25.0	41.0
38-39	34.54164378480597	37.0	33.0	40.0	25.0	41.0
40-41	34.487267207951575	37.0	33.0	40.0	25.0	41.0
42-43	34.47787582048865	37.0	33.0	40.0	24.5	41.0
44-45	34.5699929208772	37.0	33.0	40.0	25.0	41.0
46-47	34.374417826642	37.0	32.5	40.0	24.0	41.0
48-49	34.313179944172326	37.0	32.0	40.0	24.0	41.0
50-51	34.24326158083957	37.0	32.0	40.0	24.0	41.0
52-53	34.32041282148447	37.0	32.0	40.0	24.0	41.0
54-55	34.40539630719871	37.0	32.0	40.0	24.5	41.0
56-57	34.35003799994166	37.0	32.0	40.0	25.5	41.0
58-59	34.01306982646777	36.0	31.0	39.0	24.0	41.0
60-61	33.631363838757416	36.0	31.0	39.0	23.0	40.0
62-63	33.54379288112206	36.0	31.0	39.0	23.5	40.0
64-65	33.41678256252301	35.5	31.0	39.0	23.5	40.0
66-67	33.14410667635372	35.0	31.0	38.5	23.0	40.0
68-69	32.980168179982556	35.0	30.5	38.0	23.0	40.0
70-71	33.01040460381175	35.0	31.0	38.0	24.0	40.0
72-73	32.81453883106667	34.5	30.5	37.5	24.0	40.0
74-75	32.42435301981096	34.0	30.0	37.0	23.0	39.0
76-77	31.69702156259632	34.0	29.0	36.0	22.5	39.0
78-79	31.95413055585805	34.0	30.0	36.0	23.5	39.0
80-81	31.936300945002024	34.0	30.0	36.0	24.5	38.5
82-83	31.976066132095962	34.0	30.0	35.5	25.0	37.0
84-85	31.685135526062446	34.0	30.0	35.0	25.0	37.0
86-87	31.492759071885445	34.0	30.0	35.0	24.5	36.5
88-89	31.49809834177435	34.0	30.0	35.0	25.0	36.0
90-91	31.359861099937312	34.0	30.0	35.0	25.0	36.0
92-93	31.268344885639657	34.0	30.0	35.0	25.0	35.5
94-95	31.496904621337475	34.0	30.0	35.0	25.0	35.0
96-97	31.736247285421562	34.0	30.5	35.0	25.5	35.0
98-99	32.24842285813459	34.0	31.0	35.0	26.5	35.0
100	31.89448634308921	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	16.0
19	223.0
20	1409.0
21	7155.0
22	25150.0
23	65793.0
24	135300.0
25	231443.0
26	345010.0
27	463947.0
28	575464.0
29	675531.0
30	768688.0
31	854034.0
32	941151.0
33	1025758.0
34	1109000.0
35	1185990.0
36	1257452.0
37	1307111.0
38	1115111.0
39	426162.0
40	10845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.90107970765364	23.403864526914386	15.43972445794905	25.25533130748292
2	14.3079649069725	24.041126812061343	39.29532134213785	22.3555869388283
3	16.902773711242112	28.201943633062033	31.77478620406939	23.120496451626472
4	12.220728573616983	21.325217581720302	38.966540652737955	27.487513191924755
5	11.966252151626486	39.58160982370951	36.36346147890137	12.088676545762633
6	27.191582051561227	41.10508482477401	19.321555068173684	12.381778055491075
7	23.748980462720258	34.057861344845705	24.934357683930592	17.258800508503448
8	22.888105223742215	38.025636381589244	21.86903099784215	17.217227396826388
9	24.051547772804806	17.045132123480773	22.569694204040992	36.33362589967343
10-11	22.74930956764967	27.92447459757766	30.2769374465215	19.049278388251174
12-13	23.532895627251023	25.39975925779655	30.11890592610654	20.948439188845892
14-15	20.80137742294751	26.951839088327368	27.52127927278199	24.725504215943136
16-17	20.32407044878569	29.76442117767619	28.346088627804267	21.565419745733845
18-19	20.345708056986936	29.157569131749604	29.943106652428426	20.553616158835034
20-21	21.068609738662918	28.227031454252575	29.735253725295436	20.969105081789074
22-23	20.686577842894764	28.305217289070967	29.765301701898316	21.24290316613596
24-25	20.6860038026471	28.38137899365761	29.74920184092589	21.18341536276941
26-27	20.877881215090106	28.45453965728626	29.537638974468777	21.12994015315486
28-29	20.682531747714535	28.38354699438091	29.69264111109059	21.241280146813963
30-31	21.00688360844877	28.359493143141272	29.584493942823503	21.049129305586455
32-33	20.887023444845227	28.485745296520076	29.542548419087012	21.084682839547686
34-35	20.939111981137135	28.53387942938412	29.609914699684865	20.917093889793883
36-37	20.987319842040893	28.441642728614312	29.428769514214025	21.142267915130766
38-39	20.99163269926059	28.41134719038231	29.456603322455933	21.140416787901167
40-41	21.159965984446515	28.343117782792444	29.418735762920388	21.078180469840653
42-43	21.06178267541999	28.430665174276037	29.226169464541112	21.281382685762857
44-45	21.068895329109765	28.723025214422304	28.947158501181764	21.26092095528617
46-47	21.168994265317174	28.49370431742117	28.757291022340343	21.58001039492131
48-49	21.161507949961013	28.593988371698813	28.76701444214666	21.477489236193513
50-51	21.049121030006393	28.66114310318324	28.702025527825043	21.587710338985325
52-53	21.060177165461898	28.686364520543062	28.68299130075012	21.570467013244922
54-55	21.133358884125137	28.667208447447084	28.68260391889061	21.516828749537172
56-57	21.254264823926544	28.648444033743548	28.59367178609265	21.50361935623726
58-59	21.018208561183474	28.69210367837377	28.797869447511804	21.491818312930953
60-61	21.150479208058904	28.744481424363094	28.601822354852334	21.503217012725667
62-63	21.024019711909357	28.910279314039784	28.549763973316043	21.515937000734812
64-65	20.915076473364856	28.881695465328615	28.65034922559704	21.55287883570949
66-67	20.986533853162623	28.949045990900522	28.470546100314024	21.593874055622823
68-69	20.9583222970367	28.905803465629486	28.516205930324958	21.61966830700886
70-71	21.02541358502919	28.65948499283298	28.590520819283466	21.724580602854367
72-73	21.067998224915215	28.6830328365425	28.53222402767234	21.716744910869952
74-75	20.993762947603354	28.738181842754074	28.600307363795558	21.667747845847014
76-77	21.06350696674136	28.69744021968561	28.542258167260947	21.696794646312078
78-79	21.012552237420497	28.682743717417093	28.501582663937803	21.803121381224617
80-81	21.04420478695286	28.663244793251046	28.522284682916165	21.770265736879924
82-83	21.116619640936662	28.665062354509764	28.59158938458283	21.626728619970752
84-85	21.202159669796686	28.80486518199139	28.538185413336258	21.45478973487566
86-87	21.011631244326313	28.82464417812732	28.573763234730958	21.589961342815407
88-89	21.019310500588833	28.778125520942567	28.597356434951415	21.60520754351719
90-91	20.89169851330408	28.668183937193252	28.801377369933018	21.638740179569655
92-93	20.86838800510892	28.715731310993885	28.878821443620833	21.537059240276356
94-95	20.623649007908824	28.85321023302774	28.996931686330175	21.526209072733266
96-97	20.62419498161797	29.03206382350556	29.05554816322867	21.288193031647793
98-99	21.652911747405383	31.05430155907139	29.1193519797168	18.173434713806426
100	21.03200822471128	31.857652827683232	22.85437259521522	24.25596635239027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2713.0
1	2492.0
2	3787.5
3	5505.5
4	6723.0
5	10427.0
6	14237.5
7	17134.5
8	19244.0
9	19102.5
10	17250.0
11	15065.0
12	15405.5
13	19649.0
14	31116.5
15	48175.0
16	62224.0
17	69920.0
18	73481.0
19	74921.0
20	75413.5
21	76723.5
22	80347.0
23	89827.0
24	107105.0
25	131429.0
26	161014.5
27	191309.5
28	221323.5
29	254153.0
30	285131.0
31	313794.0
32	344712.0
33	372513.0
34	399272.0
35	425641.0
36	451328.5
37	475705.0
38	490394.0
39	497386.0
40	505270.5
41	511472.0
42	512919.5
43	514838.0
44	516381.5
45	517246.0
46	513104.5
47	505143.0
48	494479.0
49	474049.0
50	453350.0
51	433722.0
52	408472.5
53	379096.0
54	347433.0
55	314328.5
56	281884.0
57	249896.5
58	217770.0
59	186155.5
60	152506.0
61	120948.5
62	95085.5
63	73334.5
64	55281.0
65	41909.5
66	31874.0
67	24391.0
68	18796.0
69	14117.5
70	10697.0
71	8068.0
72	5962.5
73	4485.0
74	3321.5
75	2399.5
76	1727.5
77	1221.0
78	881.0
79	671.5
80	528.5
81	399.5
82	294.5
83	198.0
84	131.5
85	93.5
86	71.5
87	55.0
88	36.5
89	24.5
90	15.5
91	12.0
92	12.5
93	9.0
94	4.5
95	2.0
96	1.0
97	1.5
98	2.0
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012835512350468876
3	0.9270704228207747
4	0.26626504071802876
5	8.780512180047116E-5
6	2.7937993300149915E-4
7	7.982283800042832E-6
8	0.0
9	0.014439951394277483
10-11	0.07872128283602242
12-13	0.0018718455511100443
14-15	3.991141900021416E-6
16-17	3.192913520017133E-5
18-19	0.03721340707579969
20-21	0.027591750354058205
22-23	4.7122978434208855E-4
24-25	1.1189180062879195E-4
26-27	0.0014842072547010465
28-29	7.651175613147588E-4
30-31	4.976667656247599E-4
32-33	0.0013681726975182634
34-35	0.016987229455557074
36-37	4.0558074234497784E-6
38-39	5.300608734139203E-5
40-41	0.001554864714257096
42-43	7.43496143568114E-5
44-45	6.203334737895993E-4
46-47	0.0015627426031863817
48-49	0.005046868714587964
50-51	0.006301366550663138
52-53	0.006139399386903314
54-55	0.015536049375003275
56-57	0.008504365450570057
58-59	0.00969179732245715
60-61	0.010914415901957056
62-63	0.009185433510464724
64-65	0.025909765667068905
66-67	0.003823703140357355
68-69	0.0027973325157563704
70-71	0.001976551138507574
72-73	6.148226008025484E-4
74-75	9.170707851704249E-5
76-77	3.422197704076894E-5
78-79	0.0
80-81	0.0
82-83	4.084382734640258E-5
84-85	5.1882187174536554E-5
86-87	4.2215398689349074E-5
88-89	1.61324122586329E-5
90-91	5.500675620483086E-5
92-93	0.0023960882759513414
94-95	6.373966772742994E-5
96-97	2.1655594241536873E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1381.0
20-21	4332.0
22-23	7275.0
24-25	12834.0
26-27	15339.0
28-29	20818.0
30-31	31450.0
32-33	39087.0
34-35	53100.0
36-37	63030.0
38-39	71663.0
40-41	80840.0
42-43	91584.0
44-45	105285.0
46-47	105645.0
48-49	97249.0
50-51	85463.0
52-53	79598.0
54-55	85456.0
56-57	96260.0
58-59	108944.0
60-61	111443.0
62-63	112697.0
64-65	122123.0
66-67	131140.0
68-69	134994.0
70-71	135392.0
72-73	131482.0
74-75	133363.0
76-77	128790.0
78-79	146643.0
80-81	151040.0
82-83	155876.0
84-85	160426.0
86-87	169339.0
88-89	204272.0
90-91	216880.0
92-93	231076.0
94-95	281526.0
96-97	556901.0
98-99	1017777.0
100-101	6837930.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.94451106092613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.43721582795659	81.29345749822944
2	4.941055139930325	8.690773568124968
3	1.3764268599152416	3.6314756161911537
4	0.5453786831590305	1.9185224653389072
5	0.2855037082640519	1.255424201468167
6	0.14691869043448885	0.7752415437583612
7	0.08758973005040832	0.5392125188269168
8	0.0463782588878483	0.32629706413990966
9	0.034603680502599064	0.273888338643862
>10	0.0980394359256603	1.231643136255499
>50	7.424860295958915E-4	0.039252868132985744
>100	1.4749894418011222E-4	0.024811180889813773
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	7.982283800042832E-6	0.0	0.0	7.982283800042832E-6
2	0.0	1.5964567600085664E-5	0.0	1.5964567600085664E-5	7.982283800042832E-6
3	0.0	1.5964567600085664E-5	0.0	3.991141900021417E-5	7.982283800042832E-6
4	0.0	2.39468514001285E-5	0.0	4.7893702800257E-5	7.982283800042832E-6
5	0.0	4.7893702800257E-5	0.0	5.587598660029983E-5	7.982283800042832E-6
6	0.0	4.7893702800257E-5	0.0	5.587598660029983E-5	7.982283800042832E-6
7	0.0	5.587598660029983E-5	0.0	5.587598660029983E-5	7.982283800042832E-6
8	0.0	7.18405542003855E-5	0.0	5.587598660029983E-5	7.982283800042832E-6
9	0.0	1.1973425700064249E-4	0.0	6.385827040034266E-5	7.982283800042832E-6
10-11	0.0	1.1973425700064249E-4	0.0	8.780512180047117E-5	7.982283800042832E-6
12-13	0.0	1.1973425700064249E-4	0.0	9.97785475005354E-5	7.982283800042832E-6
14-15	0.0	1.7161910170092093E-4	0.0	1.1973425700064249E-4	7.982283800042832E-6
16-17	0.0	1.8359252740098516E-4	0.0	1.3968996650074957E-4	1.5964567600085664E-5
18-19	3.991141900021416E-6	2.2749508830122075E-4	0.0	1.5964567600085667E-4	1.9955709500107084E-5
20-21	7.982283800042832E-6	2.8337107490152057E-4	0.0	1.7561024360094232E-4	2.39468514001285E-5
22-23	7.982283800042832E-6	3.1929135200171334E-4	0.0	2.514419397013492E-4	2.39468514001285E-5
24-25	7.982283800042832E-6	3.5122048720188465E-4	0.0	3.153002101016919E-4	2.39468514001285E-5
26-27	7.982283800042832E-6	3.592027710019275E-4	0.0	5.148573051027628E-4	2.39468514001285E-5
28-29	7.982283800042832E-6	3.592027710019275E-4	0.0	0.0015525541991083312	2.39468514001285E-5
30-31	7.982283800042832E-6	3.951230481021202E-4	0.0	0.005427952984029126	2.39468514001285E-5
32-33	7.982283800042832E-6	4.0709647380218446E-4	0.0	0.01406079291377545	2.39468514001285E-5
34-35	7.982283800042832E-6	4.509990347024201E-4	0.0	0.024609380955532056	2.39468514001285E-5
36-37	7.982283800042832E-6	4.949015956026557E-4	0.0	0.04515977059874233	2.39468514001285E-5
38-39	7.982283800042832E-6	4.988927375026771E-4	0.0	0.09344460530520142	2.39468514001285E-5
40-41	7.982283800042832E-6	5.268307308028269E-4	0.0	0.14757247175329186	2.39468514001285E-5
42-43	7.982283800042832E-6	5.348130146028698E-4	0.0	0.1868453080495026	2.39468514001285E-5
44-45	7.982283800042832E-6	5.667421498030411E-4	0.0	0.23819933087707817	2.39468514001285E-5
46-47	7.982283800042832E-6	5.906890012031697E-4	0.0	0.2913693232691635	2.39468514001285E-5
48-49	7.982283800042832E-6	5.906890012031697E-4	0.0	0.33903553098111927	2.39468514001285E-5
50-51	7.982283800042832E-6	6.026624269032339E-4	0.0	0.38431104469496224	2.39468514001285E-5
52-53	7.982283800042832E-6	6.186269945033196E-4	0.0	0.47511750520424945	2.39468514001285E-5
54-55	1.1973425700064248E-5	6.824852649036622E-4	0.0	0.5804277753782145	2.39468514001285E-5
56-57	1.5964567600085664E-5	7.024409744037693E-4	0.0	0.6846883752324741	2.39468514001285E-5
58-59	1.5964567600085664E-5	7.104232582038121E-4	0.0	0.7708730934215364	2.39468514001285E-5
60-61	1.5964567600085664E-5	7.223966839038764E-4	0.0	0.8097308509601451	2.39468514001285E-5
62-63	1.5964567600085664E-5	7.423523934039835E-4	0.0	0.8594085941897116	2.39468514001285E-5
64-65	1.5964567600085664E-5	8.461220828045403E-4	0.0	0.8785501107422143	2.39468514001285E-5
66-67	1.5964567600085664E-5	8.700689342046688E-4	0.0	0.8861133246427548	2.39468514001285E-5
68-69	1.5964567600085664E-5	8.980069275048187E-4	0.0	0.8938721044963965	2.39468514001285E-5
70-71	1.5964567600085664E-5	9.2993606270499E-4	0.0	0.9001302149956301	2.39468514001285E-5
72-73	1.5964567600085664E-5	9.339272046050114E-4	0.0	0.9052029563505573	2.39468514001285E-5
74-75	1.5964567600085664E-5	9.419094884050542E-4	0.0	0.9095972035824809	2.39468514001285E-5
76-77	1.5964567600085664E-5	9.698474817052042E-4	0.0	0.9136881240300029	2.39468514001285E-5
78-79	1.5964567600085664E-5	9.898031912053114E-4	0.0	0.9160947825957158	2.39468514001285E-5
80-81	1.5964567600085664E-5	0.0010017766169053755	0.0	0.91688103755002	2.39468514001285E-5
82-83	1.5964567600085664E-5	0.0010057677588053968	0.0	0.9178189558965251	2.39468514001285E-5
84-85	1.5964567600085664E-5	0.0010097589007054182	0.0	0.9183058752083276	2.39468514001285E-5
86-87	1.5964567600085664E-5	0.0010217323264054825	0.0	0.9184735031681286	2.39468514001285E-5
88	1.5964567600085664E-5	0.001045679177805611	0.0	0.9184814854519285	2.39468514001285E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13525	0.0	46.346893	1
GTATCAA	23290	0.0	34.256634	1
ATCAACG	29930	0.0	25.79476	3
TCAACGC	30505	0.0	25.26711	4
CAACGCA	30840	0.0	25.00582	5
TATCAAC	32390	0.0	24.475393	2
AACGCAG	31840	0.0	23.905575	6
ACGCAGA	36670	0.0	20.688301	7
CGCAGAG	37055	0.0	20.22464	8
AGAGTAC	39780	0.0	17.615133	10-11
GTACATG	29255	0.0	17.332434	1
GCAGAGT	42840	0.0	16.985083	9
CAGAGTA	42810	0.0	16.615358	9
TACATGG	29225	0.0	16.495749	2
ACATGGG	29050	0.0	16.158417	3
GAGTACT	27610	0.0	16.066494	12-13
AGTACTT	31410	0.0	15.441773	12-13
GTGGTAT	6520	0.0	14.4114685	1
CATGGGG	20490	0.0	13.917079	4
GTACTTT	32570	0.0	13.7806425	14-15
>>END_MODULE