FastQCFastQC Report
Fri 27 May 2016
SRR2139182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11587102
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117250.046.3099561
GTATCAA205250.033.3103681
ATCAACG253350.026.0351663
TCAACGC255450.025.8345134
CAACGCA258250.025.4722255
TATCAAC273750.024.604712
AACGCAG267200.024.448036
ACGCAGA308050.021.1381767
CGCAGAG311550.020.6461478
AGAGTAC333700.017.24694310-11
GTACATG253600.017.212151
GCAGAGT361900.017.1739529
TACATGG248700.016.7357232
GAGTACT235150.016.14501212-13
ACATGGG252250.016.0476953
CAGAGTA365350.015.6293569
AGTACTT264900.014.85126212-13
GTGGTAT56200.014.135321
CATGGGG166950.013.8941064
GTACTTT279650.013.84379414-15
Produced by FastQC (version 0.11.5)