##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11587102
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.863019588504528	31.0	30.0	31.0	26.0	34.0
2	30.37118565107997	31.0	30.0	31.0	27.0	34.0
3	30.125689926609777	31.0	30.0	32.0	26.0	34.0
4	33.98658586072687	35.0	35.0	37.0	30.0	37.0
5	33.715050406909334	35.0	33.0	37.0	28.0	37.0
6	33.71127664190753	35.0	33.0	37.0	28.0	37.0
7	33.66884195892985	35.0	33.0	37.0	28.0	37.0
8	33.54937170657512	35.0	33.0	37.0	28.0	37.0
9	34.83305221616242	37.0	33.0	39.0	28.0	39.0
10-11	34.86345960361788	37.0	33.0	39.0	28.0	39.0
12-13	34.7587086054822	37.0	33.0	39.0	27.0	39.0
14-15	35.54402813576682	38.0	33.0	40.0	27.0	41.0
16-17	35.4367415597101	38.0	33.0	40.0	27.0	41.0
18-19	35.63497102208991	38.0	33.5	40.0	27.0	41.0
20-21	35.61541774751193	38.0	34.0	40.0	27.0	41.0
22-23	35.54688621128123	38.0	34.0	40.0	27.0	41.0
24-25	35.53435330345201	38.0	34.0	40.0	27.0	41.0
26-27	35.25294887125921	38.0	33.0	40.0	27.0	41.0
28-29	34.98199445011264	37.5	33.0	40.0	26.0	41.0
30-31	34.97411495198517	37.0	33.0	40.0	26.0	41.0
32-33	34.93726081509328	37.0	33.0	40.0	25.5	41.0
34-35	34.71830497127553	37.0	33.0	40.0	25.0	41.0
36-37	34.57562851329076	37.0	33.0	40.0	25.0	41.0
38-39	34.5655423369917	37.0	33.0	40.0	25.0	41.0
40-41	34.51308184209036	37.0	33.0	40.0	25.0	41.0
42-43	34.51129409692777	37.0	33.0	40.0	25.0	41.0
44-45	34.60459053565901	37.0	33.0	40.0	25.0	41.0
46-47	34.412675251909064	37.0	32.5	40.0	24.0	41.0
48-49	34.36770994131375	37.0	32.0	40.0	24.0	41.0
50-51	34.30670792788341	37.0	32.0	40.0	24.0	41.0
52-53	34.36823677135834	37.0	32.0	40.0	24.0	41.0
54-55	34.4736821716494	37.0	32.0	40.0	25.0	41.0
56-57	34.43192131084409	37.0	32.0	40.0	25.5	41.0
58-59	34.115531949825666	36.0	31.5	39.0	24.5	41.0
60-61	33.75336006378197	36.0	31.0	39.0	23.5	40.5
62-63	33.686994371375334	36.0	31.0	39.0	23.5	40.0
64-65	33.550745353149544	35.5	31.0	39.0	24.0	40.0
66-67	33.30291839964498	35.0	31.0	39.0	23.0	40.0
68-69	33.13349151269962	35.0	31.0	38.0	23.5	40.0
70-71	33.120624289804766	35.0	31.0	38.0	24.5	40.0
72-73	32.91469119010145	35.0	31.0	37.5	24.5	40.0
74-75	32.52739501012086	34.0	30.0	37.0	23.5	39.5
76-77	31.780731171285424	34.0	29.5	36.0	22.5	39.0
78-79	32.05562575082266	34.0	30.0	36.0	24.0	39.0
80-81	32.039251066996805	34.0	30.0	36.0	24.5	38.5
82-83	32.08823977738891	34.0	30.0	35.5	25.0	37.0
84-85	31.78220333058193	34.0	30.0	35.0	25.0	37.0
86-87	31.61436600243711	34.0	30.0	35.0	25.0	36.5
88-89	31.59989600393031	34.0	30.0	35.0	25.0	36.0
90-91	31.452528034498293	34.0	30.0	35.0	25.0	36.0
92-93	31.366292593094308	34.0	30.0	35.0	25.0	36.0
94-95	31.56263090636201	34.0	30.0	35.0	25.0	35.0
96-97	31.784739989956226	34.0	30.5	35.0	25.5	35.0
98-99	32.262807760776134	34.0	31.0	35.0	26.5	35.0
100	31.895168794744766	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	29.0
19	185.0
20	1323.0
21	6252.0
22	21978.0
23	58169.0
24	119533.0
25	206278.0
26	309917.0
27	416437.0
28	522141.0
29	619466.0
30	707392.0
31	788648.0
32	871668.0
33	950146.0
34	1029319.0
35	1100746.0
36	1169504.0
37	1214748.0
38	1050543.0
39	411374.0
40	11300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.0324436602008	23.39721355693598	15.392347456680714	25.177995326182508
2	14.24188939970449	24.041720117589442	39.36882077553857	22.34756970716749
3	16.77306122156918	28.313883876397146	31.788363101556822	23.124691800476853
4	12.172977330156224	21.140423324730946	39.242469347231044	27.444129997881795
5	11.893226087631934	39.64020332956478	36.46562125121903	12.000949331584263
6	27.239867787213672	41.18830121033955	19.327027976593584	12.244803025853194
7	23.742539225298888	34.100634835236185	25.11624780003212	17.040578139432803
8	22.84030122458575	38.1308372015712	21.93087624498343	17.097985328859625
9	24.065257003073732	17.09414627367338	22.594320201428193	36.2462765218247
10-11	22.671204702068547	28.05163761620286	30.354371546134495	18.922786135594095
12-13	23.437918712266	25.507878964672496	30.2593712794617	20.7948310435998
14-15	20.67990339603466	27.077646334691796	27.649769545482556	24.592680723790988
16-17	20.235448051516258	29.87563885163841	28.49665699340892	21.392256103436413
18-19	20.30996115232531	29.3318636842322	29.9251124793568	20.43306268408568
20-21	21.048354695872774	28.366482657165292	29.79908376141224	20.786078885549696
22-23	20.71415461878678	28.41874471844113	29.723578217196568	21.143522445575524
24-25	20.635039536720406	28.4946696426018	29.748296574348227	21.121994246329567
26-27	20.80368648927282	28.62330936446461	29.5213505710841	21.05165357517847
28-29	20.758962609750064	28.5630598992689	29.646915515885592	21.03106197509545
30-31	21.11504831506476	28.57405214395633	29.462022387265357	20.848877153713552
32-33	20.94184021265676	28.61207697696043	29.479931959786636	20.96615085059617
34-35	20.916625782853554	28.62903033982643	29.536701181901176	20.917642695418845
36-37	20.976532986673966	28.558929316311538	29.447645767803564	21.01689192921093
38-39	20.952667992480364	28.62230785669167	29.408149411476366	21.0168747393516
40-41	21.117588112066493	28.582127415850017	29.310975333428345	20.98930913865514
42-43	21.007910165199295	28.69091795019658	29.18027085442748	21.120901030176643
44-45	21.107276107375736	28.861487160571592	28.89129031190659	21.13994642014608
46-47	21.088427632893893	28.741794876965315	28.724083595971766	21.44569389416902
48-49	21.122525666670004	28.844060088487794	28.74097349069193	21.29244075415027
50-51	21.06395602051737	28.930600446485577	28.65113330927272	21.35431022372434
52-53	21.13716026214207	28.926675158740373	28.584080829288478	21.352083749829077
54-55	21.147199812600494	28.860004416331375	28.63508259175965	21.357713179308472
56-57	21.257344045953957	28.90768502962352	28.561749917024088	21.273221007398433
58-59	21.08647248046071	28.96106515824718	28.702112212270702	21.250350149021404
60-61	21.19941969592047	28.904832606739618	28.57773800343648	21.318009693903434
62-63	21.1511155539903	29.03164145310926	28.519520600691216	21.297722392209224
64-65	21.04142712857913	28.995986045964052	28.678637064943274	21.283949760513547
66-67	21.11721673397685	28.995554882052836	28.550832617736692	21.336395766233622
68-69	21.046626415120365	28.93280458425009	28.652480953135846	21.3680880474937
70-71	21.10530254045223	28.74846083260888	28.752193703442767	21.39404292349612
72-73	21.13394528135458	28.80987297169491	28.70126341988804	21.354918327062464
74-75	20.982378342287024	28.910689637689437	28.728409516704968	21.378522503318575
76-77	21.03569437230587	28.89462336144565	28.71510827878217	21.35457398746631
78-79	20.990238598354864	28.856492658852908	28.700198732923653	21.45307000986858
80-81	21.045037525150057	28.85814990085783	28.71518937870358	21.381623195288533
82-83	21.087564304657093	28.84476122822925	28.806020935007354	21.261653532106305
84-85	21.098982303386666	28.93959970599876	28.76663651269294	21.194781477921637
86-87	20.967464395362637	28.94301699976733	28.78229688165026	21.307221723219776
88-89	21.04110961959873	28.904262128438546	28.71772934873399	21.336898903228732
90-91	20.9506806318541	28.961451359643238	28.826889150201485	21.260978858301176
92-93	20.924353928375606	28.945738460122943	28.871413469328406	21.258494142173046
94-95	20.725985589054357	29.088420045692626	28.889204683251425	21.296389682001596
96-97	20.68697555385866	29.24846992730866	28.99781261547757	21.066741903355112
98-99	21.715691756102114	31.21275534946889	29.112287424844386	17.959265469584608
100	20.95055212641244	32.14703488842462	22.94025359266856	23.96215939249438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1709.0
1	1594.0
2	2926.5
3	4583.0
4	5678.5
5	8789.0
6	12036.5
7	14491.5
8	16149.5
9	16116.0
10	14662.5
11	12814.5
12	13184.5
13	16860.5
14	26885.5
15	41760.5
16	54210.5
17	61251.0
18	64133.5
19	65201.0
20	66094.0
21	67838.0
22	72285.5
23	82315.0
24	99507.5
25	124397.0
26	154254.5
27	183867.0
28	213427.5
29	245542.5
30	275566.5
31	304374.0
32	335179.0
33	362891.0
34	388367.0
35	411138.5
36	432378.0
37	452671.5
38	465162.0
39	471837.0
40	478303.5
41	481500.0
42	481669.0
43	481921.0
44	480949.0
45	479157.0
46	473232.0
47	464052.5
48	452974.5
49	434718.5
50	415174.5
51	395067.0
52	369394.5
53	340101.5
54	310552.5
55	282165.0
56	253859.0
57	225680.0
58	196972.0
59	168468.0
60	139316.5
61	111142.0
62	87622.0
63	67769.5
64	50795.5
65	37719.5
66	27847.5
67	21122.0
68	16365.0
69	12215.5
70	9015.5
71	6755.0
72	5073.0
73	3739.5
74	2700.5
75	1909.5
76	1326.5
77	970.5
78	728.5
79	562.0
80	427.5
81	314.0
82	237.5
83	157.5
84	112.0
85	89.0
86	69.0
87	50.5
88	29.0
89	16.0
90	10.5
91	10.0
92	9.0
93	5.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014740527873147228
3	1.099489760252391
4	0.3010502539806761
5	1.7260571280031884E-5
6	1.9849656972036666E-4
7	8.630285640015942E-6
8	0.0
9	0.015810683292509203
10-11	0.09130842207136866
12-13	0.0020453776966837785
14-15	0.0
16-17	2.1575714100039855E-5
18-19	0.04486885504244288
20-21	0.02416863457615789
22-23	5.655793583394245E-4
24-25	1.425585290240741E-4
26-27	0.0014659599557686584
28-29	8.399527963847046E-4
30-31	6.462844736921664E-4
32-33	0.0014003427186592753
34-35	0.019147458953725768
36-37	4.383506111418468E-6
38-39	1.7635579358101065E-5
40-41	0.0015445968382546572
42-43	1.1622721454864323E-4
44-45	5.499870617797762E-4
46-47	0.0013018145519652735
48-49	0.004956989274480992
50-51	0.006386300060910843
52-53	0.005640190869292665
54-55	0.015447703233315504
56-57	0.007733356162429015
58-59	0.00919394423618234
60-61	0.010178800311159664
62-63	0.008641939341433215
64-65	0.02373357655334747
66-67	0.0035778551810499953
68-69	0.002636981824197087
70-71	0.0019819251506969114
72-73	6.603150940439475E-4
74-75	1.210917250864042E-4
76-77	4.7988682349154774E-5
78-79	0.0
80-81	5.48140463624878E-6
82-83	3.895732608934841E-5
84-85	6.784639485742418E-5
86-87	5.1743169901573E-5
88-89	3.514953314390078E-5
90-91	1.7971977373999365E-5
92-93	0.002301695434995272
94-95	6.30747330251544E-5
96-97	1.2437604140386774E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1117.0
20-21	3578.0
22-23	6001.0
24-25	10923.0
26-27	13011.0
28-29	18159.0
30-31	28946.0
32-33	35528.0
34-35	49571.0
36-37	61286.0
38-39	74857.0
40-41	77936.0
42-43	89730.0
44-45	104803.0
46-47	103469.0
48-49	96730.0
50-51	89516.0
52-53	88536.0
54-55	95157.0
56-57	103115.0
58-59	107063.0
60-61	107866.0
62-63	104256.0
64-65	109011.0
66-67	117310.0
68-69	120830.0
70-71	122746.0
72-73	119212.0
74-75	121278.0
76-77	117557.0
78-79	132805.0
80-81	136151.0
82-83	140378.0
84-85	144980.0
86-87	154510.0
88-89	185795.0
90-91	195964.0
92-93	211002.0
94-95	256193.0
96-97	508470.0
98-99	917946.0
100-101	6303810.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.67881583571362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.92892970424631	83.33756372757082
2	4.97026913324094	8.914557005076924
3	1.2105111082319637	3.2567160822665966
4	0.4324508124338335	1.5512670706503398
5	0.1974692806246942	0.8854405625176403
6	0.0981373274540361	0.5280503589215775
7	0.052185554067359585	0.3275957084743991
8	0.034775378170971705	0.24948917876894938
9	0.019045034971316734	0.1537142565399695
>10	0.05560734317577971	0.7452247581757891
>50	5.486342842682406E-4	0.031509297053958406
>100	5.7431584179459993E-5	0.012416168248671134
>500	1.325751429742916E-5	0.0064558257343334
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5890856920047827E-5	0.0	8.630285640015942E-6	0.0
3	0.0	2.5890856920047827E-5	0.0	8.630285640015942E-6	0.0
4	0.0	6.041199948011159E-5	0.0	8.630285640015942E-6	0.0
5	0.0	6.904228512012753E-5	0.0	1.7260571280031884E-5	0.0
6	0.0	6.904228512012753E-5	0.0	1.7260571280031884E-5	0.0
7	0.0	7.767257076014348E-5	0.0	2.5890856920047827E-5	0.0
8	0.0	8.630285640015942E-5	0.0	2.5890856920047827E-5	8.630285640015942E-6
9	0.0	1.46714855880271E-4	0.0	4.315142820007971E-5	1.7260571280031884E-5
10-11	0.0	1.5102999870027898E-4	0.0	6.041199948011159E-5	1.7260571280031884E-5
12-13	0.0	1.5534514152028696E-4	0.0	8.630285640015942E-5	1.7260571280031884E-5
14-15	0.0	1.8123599844033477E-4	0.0	9.493314204017536E-5	3.0205999740055795E-5
16-17	0.0	1.8986628408035073E-4	0.0	9.493314204017536E-5	3.452114256006377E-5
18-19	0.0	2.243874266404145E-4	0.0	9.924828486018334E-5	3.452114256006377E-5
20-21	0.0	2.4164799792044636E-4	0.0	1.1219371332020724E-4	3.452114256006377E-5
22-23	0.0	2.8048428330051814E-4	0.0	1.1219371332020724E-4	3.452114256006377E-5
24-25	0.0	3.02059997400558E-4	0.0	1.4239971306026305E-4	4.315142820007971E-5
26-27	0.0	3.236357115005978E-4	0.0	1.7692085562032682E-4	4.315142820007971E-5
28-29	0.0	3.2795085432060577E-4	0.0	4.056234250807493E-4	4.746657102008768E-5
30-31	0.0	3.5384171124065364E-4	0.0	0.0011909794183222	5.1781713840095654E-5
32-33	0.0	3.6247199688066954E-4	0.0	0.0028221034042852132	5.1781713840095654E-5
34-35	0.0	4.3582942482080504E-4	0.0	0.005018511099669271	5.1781713840095654E-5
36-37	0.0	5.091868527609405E-4	0.0	0.008949606208696531	5.1781713840095654E-5
38-39	4.315142820007971E-6	5.135019955809486E-4	0.0	0.01567259872226895	5.1781713840095654E-5
40-41	8.630285640015942E-6	5.350777096809884E-4	0.0	0.02324998951420295	5.1781713840095654E-5
42-43	8.630285640015942E-6	5.566534237810283E-4	0.0	0.030279357167995933	5.1781713840095654E-5
44-45	8.630285640015942E-6	6.213805660811478E-4	0.0	0.038327098527310796	5.1781713840095654E-5
46-47	8.630285640015942E-6	6.645319942812276E-4	0.0	0.046586281884806055	5.1781713840095654E-5
48-49	8.630285640015942E-6	6.645319942812276E-4	0.0	0.05450456895952068	5.1781713840095654E-5
50-51	8.630285640015942E-6	7.076834224813073E-4	0.0	0.06181873603943419	5.1781713840095654E-5
52-53	8.630285640015942E-6	7.076834224813073E-4	0.0	0.07104882653143124	5.1781713840095654E-5
54-55	8.630285640015942E-6	7.163137081213232E-4	0.0	0.07992939045500765	5.1781713840095654E-5
56-57	8.630285640015942E-6	7.249439937613391E-4	0.0	0.0888919420921642	6.041199948011159E-5
58-59	8.630285640015942E-6	7.46519707861379E-4	0.0	0.096905162308919	6.904228512012753E-5
60-61	8.630285640015942E-6	7.55149993501395E-4	4.315142820007971E-6	0.10474146167005348	6.904228512012753E-5
62-63	8.630285640015942E-6	7.594651363214029E-4	8.630285640015942E-6	0.11296612388498867	6.904228512012753E-5
64-65	8.630285640015942E-6	7.853559932414507E-4	8.630285640015942E-6	0.12031481210746225	6.904228512012753E-5
66-67	8.630285640015942E-6	7.896711360614587E-4	8.630285640015942E-6	0.12633012119855336	6.904228512012753E-5
68-69	8.630285640015942E-6	8.155619929815065E-4	8.630285640015942E-6	0.13271653257216515	6.904228512012753E-5
70-71	8.630285640015942E-6	8.371377070815464E-4	8.630285640015942E-6	0.13767894681517434	6.904228512012753E-5
72-73	8.630285640015942E-6	8.457679927215623E-4	8.630285640015942E-6	0.14201998049210235	6.904228512012753E-5
74-75	8.630285640015942E-6	8.500831355415703E-4	8.630285640015942E-6	0.14585182731626942	6.904228512012753E-5
76-77	8.630285640015942E-6	8.630285640015942E-4	8.630285640015942E-6	0.14923489928715566	6.904228512012753E-5
78-79	8.630285640015942E-6	8.716588496416101E-4	8.630285640015942E-6	0.15099547755771892	6.904228512012753E-5
80-81	8.630285640015942E-6	8.75973992461618E-4	8.630285640015942E-6	0.15178083355096036	7.33574279401355E-5
82-83	8.630285640015942E-6	8.802891352816261E-4	8.630285640015942E-6	0.15263091668650192	7.767257076014348E-5
84-85	8.630285640015942E-6	8.84604278101634E-4	8.630285640015942E-6	0.15299770382620262	7.767257076014348E-5
86-87	8.630285640015942E-6	8.88919420921642E-4	8.630285640015942E-6	0.15312715811080285	8.198771358015146E-5
88	8.630285640015942E-6	9.407011347617376E-4	8.630285640015942E-6	0.15313578839644287	8.630285640015942E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11725	0.0	46.309956	1
GTATCAA	20525	0.0	33.310368	1
ATCAACG	25335	0.0	26.035166	3
TCAACGC	25545	0.0	25.834513	4
CAACGCA	25825	0.0	25.472225	5
TATCAAC	27375	0.0	24.60471	2
AACGCAG	26720	0.0	24.44803	6
ACGCAGA	30805	0.0	21.138176	7
CGCAGAG	31155	0.0	20.646147	8
AGAGTAC	33370	0.0	17.246943	10-11
GTACATG	25360	0.0	17.21215	1
GCAGAGT	36190	0.0	17.173952	9
TACATGG	24870	0.0	16.735723	2
GAGTACT	23515	0.0	16.145012	12-13
ACATGGG	25225	0.0	16.047695	3
CAGAGTA	36535	0.0	15.629356	9
AGTACTT	26490	0.0	14.851262	12-13
GTGGTAT	5620	0.0	14.13532	1
CATGGGG	16695	0.0	13.894106	4
GTACTTT	27965	0.0	13.843794	14-15
>>END_MODULE