FastQCFastQC Report
Fri 27 May 2016
SRR2139181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6585981
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85100.053.7998121
GTATCAA165450.040.5558971
ATCAACG203700.032.037993
CAACGCA205100.031.7339365
TCAACGC207150.031.56784
TATCAAC214050.030.8978232
AACGCAG214600.030.4718996
ACGCAGA240250.027.01827
CGCAGAG244500.026.5306558
CTTATAC57700.024.35051
GTACATG221550.023.4705541
ATCTCCG82750.023.0620087
GCAGAGT286950.022.6821069
TACATGG227000.022.4635682
ACATGGG223600.021.8655323
TCTCCGA89650.021.2381958
TATACAC79150.020.6270243
CATGGGG155650.020.0220954
GAGTACT172850.019.87828612-13
CTCCGAG96100.019.8582849
Produced by FastQC (version 0.11.5)